Fungal Genomics

Agaricus bisporus var bisporus H39 Agaricus bisporus var bisporus H97 Agaricus bisporus var burnettii H119p4 Aspergillus niger CBS 112.32 Aspergillus niger CBS 113.50 Aspergillus niger CBS 115988 Aspergillus niger CBS 115989 Aspergillus niger CBS 118.52 Aspergillus niger CBS 124.48 Aspergillus niger CBS 131.52 Aspergillus niger CBS 133816 Aspergillus niger CBS 147320 Aspergillus niger CBS 147321 Aspergillus niger CBS 147322 Aspergillus niger CBS 147323 Aspergillus niger CBS 147324 Aspergillus niger CBS 147343 Aspergillus niger CBS 147344 Aspergillus niger CBS 147345 Aspergillus niger CBS 147346 Aspergillus niger CBS 147347 Aspergillus niger CBS 147352 Aspergillus niger CBS 147353 Aspergillus niger CBS 147371 Aspergillus niger CBS 147482 Aspergillus niger CBS 630.78 Aspergillus niger CBS 769.97 Niveomyces coronatus CBS 149186 Ophiocordyceps australis 1348a (Ghana) Ophiocordyceps australis map64 (Brazil) Ophiocordyceps camponoti-floridani Ophiocordyceps camponoti-rufipedis Ophiocordyceps kimflemingae Ophiocordyceps subramaniannii Penicillium roqueforti DTO002I6 Penicillium roqueforti DTO003C3 Penicillium roqueforti DTO003H1 (CBS 147308) Penicillium roqueforti DTO006G1 Penicillium roqueforti DTO006G7 Penicillium roqueforti DTO012A1 Penicillium roqueforti DTO012A2 (CBS 147309) Penicillium roqueforti DTO012A6 (CBS 147310) Penicillium roqueforti DTO012A7 Penicillium roqueforti DTO012A8 Penicillium roqueforti DTO012A9 Penicillium roqueforti DTO013E5 Penicillium roqueforti DTO013F2 Penicillium roqueforti DTO013F5 (CBS 147311) Penicillium roqueforti DTO027I6 Penicillium roqueforti DTO032C6 Penicillium roqueforti DTO039G3 Penicillium roqueforti DTO046C5 Penicillium roqueforti DTO070G2 (CBS 147317) Penicillium roqueforti DTO081F9 (CBS 147318) Penicillium roqueforti DTO101D6 (CBS 147325) Penicillium roqueforti DTO102I9 (CBS 147326) Penicillium roqueforti DTO126G2 (CBS 147330) Penicillium roqueforti DTO127F7 (CBS 147331) Penicillium roqueforti DTO127F9 (CBS 147332) Penicillium roqueforti DTO130C1 (CBS 147333) Penicillium roqueforti DTO163C3 (CBS 147337) Penicillium roqueforti DTO163F5 (CBS 147338) Penicillium roqueforti DTO163G4 (CBS 147339) Penicillium roqueforti DTO265D5 (CBS 147372) Penicillium roqueforti DTO369A1 (CBS 147354) Penicillium roqueforti DTO375B1 (CBS 147355) Penicillium roqueforti DTO377G2 (LCP 96.3914a) Penicillium roqueforti LCP96 04111 Rhodonia placenta FPRL280 Torrubiellomyces zombiae CBS 149187

General Properties

Protein ID	Pro_DTO377G3_2|g8186.t1
Gene name
Location	scaffold_30:64965..68373
Strand	-
Gene length (bp)	3408
Transcript length (bp)	3306
Coding sequence length (bp)	3306
Protein length (aa)	1102

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PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF03659	Glyco_hydro_71	Glycosyl hydrolase family 71	2.4E-135	26	407

GO

GO Term	Description	Terminal node
GO:0051118	glucan endo-1,3-alpha-glucosidase activity	Yes
GO:0016798	hydrolase activity, acting on glycosyl bonds	No
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	No
GO:0015926	glucosidase activity	No
GO:0003674	molecular_function	No
GO:0003824	catalytic activity	No
GO:0016787	hydrolase activity	No

SignalP

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SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
Yes	1 - 24	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Genome-wide gene expression in conidia of Penicillium roqueforti during growth at various temperatures and for various amounts of time. Published in Punt et al., 2020.

Expression values

Label	Description	Expression (RPKM)	Confidence interval (low)	Confidence interval (high)
3d_25C	Three days - 25 degrees Celsius	5.47	2.50	8.43
5d_25C	Five days - 25 degrees Celsius	8.15	4.13	12.18
7d_25C	Seven days - 25 degrees Celsius	6.78	3.29	10.26
10d_25C	Ten days - 25 degrees Celsius	7.53	3.76	11.30
7d_15C	Seven days - 15 degrees Celsius	6.96	3.38	10.53
7d_30C	Seven days - 30 degrees Celsius	9.46	5.00	13.92

Differential expression

Label1	Label2	Q-value	Significant difference
3d_25C	5d_25C	0.170472	no
3d_25C	7d_25C	0.539536	no
3d_25C	7d_15C	0.488223	no
3d_25C	7d_30C	0.046354	yes
3d_25C	10d_25C	0.305517	no
5d_25C	7d_25C	0.587058	no
5d_25C	7d_15C	0.656088	no
5d_25C	7d_30C	0.660896	no
5d_25C	10d_25C	0.843920	no
7d_25C	7d_15C	0.955797	no
7d_25C	7d_30C	0.256929	no
7d_25C	10d_25C	0.791990	no
7d_15C	7d_30C	0.304669	no
7d_15C	10d_25C	0.851769	no
7d_30C	10d_25C	0.469855	no

Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>Pro_DTO377G3_2|g8186.t1 MVSLLAWATVLLLACRVQLQVQAAAVFAHFMVTNTANYTSNDWENDILLAQDAHIDAFALNMAYADSTNTGALAA AFSAADSLGFKLFFSFDYAGNGDWPKADVITLIQQYSAHSSYYFYKGQAFVSTFEGPGRADDWPEIKAVTGCFFI PSWSSLGAKPAVATGVVDGLFSWAGWPWGPQDADTYVDASYLQYLESLPYMMPVSPWFFTNLPGYKKNWMWRGDH LWHDRWQQVLYIQPEFVEIISWNDYGESHYIGPLYDQAMEAFEVGEAPFNYATEMPHDGWRATIPFWADMYKDGT AEVTEETIIAWYRLTPGAACDSGGTSGNTASQLQVEFPPAEIAQDRIFFSAVLGSFSGAVVSIGDWTQTVGWFDV PDDDVGVYYGDVAIGAHRGPVTVSLMRDNVIIATIEGKAISSTCEHGVQNWNAWVGSTSTGETVSARPTRLLSEQ VCMNGTGANNFAGLCGFACRYGYCPLGACTCTRMGIGYEPPNSTGVLGYPIVGEGASYSGLCSFDCNLGFCPESA CGTVEVPLSTPTVSPFLPPACVSGSGEGNLAGLCDFGCAHGFCPMNACTCTGQGALNVMDPTSETVGTAAPGVDA TVYGPLCEYTCQRGYCPEGACIVAEDSDSGSGSGDGPGDGEVYIAPSIWNMPSPVVQCEPPCSLIMPPLQLASPS VISIPPWESPVEQSYLTTRTTTYDDGVVETYNGYAVATTTITISFPPSVVTEIPVWGVTINASQAQPTELEMTRS ITLPYMVWSLPPFDEDPNATSMTGTVLPPPYPWSQASKDSELNTATTTWSSGSATPTADRGNPDVGVSCKPFCHL CLLCRPDWSGGGGGGSDDGSNSNSNSNSNEDECSTREAEICSTVCVAGSGCEFDCSTTTGCSVTASATSTVGTPA PAVAITLEQWPEATEEPDAEVTSIARSLDSRLSALYGDLTVLTGYEDGAATATATSTSRATTTTSAPPDLPTSLS DLQFVIFLRKPADSLDSWTWSGYLLPTYYDEEYVCNNAYEVVTSTDNIPNQTDEDSDYGYPMYLGPFAAQDLNCG YVSQGLTPGDVHCGTQVLTCLELDALHALGYPGYTCGNTEFYMQAWCPMAD*
Coding	>Pro_DTO377G3_2|g8186.t1 ATGGTCTCCTTGCTGGCTTGGGCCACGGTGCTGCTGCTCGCCTGCCGAGTGCAGCTGCAAGTACAGGCAGCGGCC GTCTTCGCGCACTTTATGGTCACAAACACGGCCAACTATACTTCTAATGACTGGGAGAACGATATTCTTCTGGCG CAGGATGCGCACATCGACGCATTCGCCCTCAACATGGCGTATGCCGACTCGACCAACACGGGTGCCCTCGCTGCC GCGTTTTCGGCTGCCGATAGTCTCGGCTTCAAGCTCTTTTTCTCCTTTGACTACGCAGGTAACGGCGACTGGCCT AAAGCCGACGTGATCACCCTCATTCAGCAGTACAGTGCTCACTCGTCCTACTACTTCTATAAGGGACAGGCCTTT GTCTCGACGTTCGAAGGCCCTGGACGCGCCGACGACTGGCCCGAGATCAAGGCGGTCACCGGCTGCTTCTTCATC CCCAGTTGGTCCTCGCTCGGAGCCAAGCCTGCCGTGGCGACGGGGGTCGTGGATGGTCTGTTCAGCTGGGCGGGC TGGCCCTGGGGCCCCCAGGACGCGGACACCTACGTGGACGCCTCGTATCTCCAGTATCTGGAGTCGCTGCCGTAC ATGATGCCGGTCTCGCCGTGGTTCTTCACCAACCTTCCCGGGTACAAAAAAAACTGGATGTGGCGCGGGGACCAC CTGTGGCATGATCGATGGCAGCAGGTCCTATACATTCAACCGGAGTTTGTCGAGATCATCTCGTGGAACGACTAC GGTGAGTCGCACTACATCGGCCCGCTGTACGACCAGGCCATGGAAGCGTTTGAGGTCGGCGAGGCCCCCTTCAAC TACGCGACAGAAATGCCCCACGACGGCTGGCGGGCAACGATCCCCTTCTGGGCCGATATGTATAAGGATGGAACG GCCGAGGTGACTGAGGAGACGATTATCGCCTGGTATCGGCTTACCCCCGGCGCGGCCTGTGACAGCGGCGGCACA AGCGGCAATACGGCCTCCCAGCTGCAGGTTGAGTTCCCCCCGGCGGAGATAGCCCAGGACCGTATCTTCTTCTCC GCAGTGTTGGGCTCGTTCTCGGGCGCCGTAGTGTCGATTGGAGACTGGACCCAGACGGTTGGGTGGTTCGATGTG CCGGATGATGATGTCGGGGTATACTACGGCGATGTAGCCATTGGCGCCCATCGGGGTCCGGTGACTGTGTCTTTG ATGCGTGATAATGTGATTATTGCGACGATTGAGGGCAAGGCCATCTCGTCGACCTGTGAACATGGAGTTCAGAAT TGGAATGCCTGGGTTGGAAGTACGTCTACCGGGGAGACCGTGTCGGCACGACCGACCCGTCTTCTGTCAGAGCAG GTCTGCATGAACGGTACCGGCGCGAATAATTTTGCCGGACTGTGTGGGTTTGCCTGCCGATACGGATACTGTCCT CTGGGCGCCTGTACCTGCACGCGAATGGGCATCGGGTACGAGCCGCCCAACTCCACGGGTGTCCTCGGATACCCG ATTGTGGGCGAAGGCGCGAGTTACAGCGGACTGTGCTCGTTTGACTGCAACCTGGGCTTCTGCCCCGAGAGTGCC TGCGGCACTGTGGAGGTCCCGCTCAGCACGCCGACAGTCTCGCCGTTCCTGCCCCCCGCCTGTGTGTCCGGTTCG GGTGAGGGCAACCTAGCTGGGCTGTGCGACTTTGGCTGTGCCCATGGCTTCTGCCCCATGAACGCGTGCACCTGC ACGGGGCAGGGGGCGCTAAATGTCATGGATCCGACAAGCGAGACCGTTGGGACCGCCGCACCGGGAGTAGACGCT ACCGTCTACGGTCCTCTGTGCGAGTACACTTGTCAACGCGGATACTGTCCCGAAGGCGCCTGTATCGTCGCCGAG GACTCGGACTCGGGCTCGGGGTCCGGGGATGGACCAGGCGATGGCGAGGTCTACATTGCCCCGTCGATCTGGAAT ATGCCCTCGCCGGTCGTGCAATGTGAGCCTCCGTGCTCGCTGATCATGCCTCCGCTGCAGCTGGCCAGCCCTTCT GTAATATCCATCCCTCCCTGGGAGAGCCCCGTCGAGCAGAGTTACTTGACGACCAGAACGACTACCTATGACGAC GGAGTCGTCGAAACATACAATGGCTATGCGGTTGCCACCACCACCATTACCATATCCTTTCCCCCTTCGGTCGTC ACCGAGATCCCCGTATGGGGAGTGACCATCAATGCCAGCCAGGCTCAGCCAACCGAGTTGGAAATGACGAGGAGC ATCACCCTGCCCTATATGGTATGGTCGCTTCCCCCGTTCGATGAAGACCCCAACGCGACAAGCATGACGGGGACG GTCCTTCCGCCCCCATATCCATGGTCCCAGGCCAGCAAGGACTCCGAGCTCAATACCGCGACCACCACCTGGTCG TCCGGCAGTGCGACTCCCACGGCAGATAGGGGTAACCCGGACGTCGGCGTCTCGTGTAAGCCATTCTGCCACCTG TGTCTCTTGTGCCGCCCAGACTGGAGCGGGGGCGGCGGCGGGGGCTCCGACGATGGCAGCAACAGCAACAGCAAC AGCAACAGCAACGAGGACGAGTGCAGCACGCGAGAGGCGGAGATTTGCTCTACCGTGTGTGTGGCCGGGTCCGGG TGCGAGTTCGACTGCTCCACGACCACCGGCTGTTCCGTCACGGCCTCGGCCACCAGCACCGTGGGCACGCCCGCG CCCGCCGTGGCCATCACGCTGGAGCAATGGCCTGAAGCCACCGAGGAGCCCGATGCAGAGGTGACCAGCATCGCT CGGTCGCTCGACTCCCGGCTCTCCGCCCTGTATGGAGACCTCACCGTCCTAACGGGATACGAGGATGGAGCCGCC ACGGCCACGGCCACCAGCACCAGTCGCGCGACCACGACGACCAGCGCCCCCCCGGACCTCCCAACCTCACTTTCC GACCTCCAGTTCGTGATCTTCCTGCGCAAACCCGCCGACTCCCTGGACTCCTGGACCTGGTCCGGCTACCTCCTC CCGACGTACTACGACGAGGAGTACGTGTGCAACAACGCGTACGAGGTTGTCACGAGCACCGATAATATCCCAAAT CAAACGGATGAGGACTCCGACTACGGATACCCGATGTATCTGGGTCCGTTTGCCGCGCAGGATTTGAACTGTGGG TATGTGTCCCAAGGCCTGACGCCGGGCGACGTGCACTGCGGCACTCAGGTACTGACGTGCCTCGAGCTTGATGCG CTGCATGCTCTGGGGTATCCGGGGTATACTTGTGGGAATACGGAGTTTTACATGCAGGCTTGGTGCCCGATGGCA GACTAA
Transcript	>Pro_DTO377G3_2|g8186.t1 ATGGTCTCCTTGCTGGCTTGGGCCACGGTGCTGCTGCTCGCCTGCCGAGTGCAGCTGCAAGTACAGGCAGCGGCC GTCTTCGCGCACTTTATGGTCACAAACACGGCCAACTATACTTCTAATGACTGGGAGAACGATATTCTTCTGGCG CAGGATGCGCACATCGACGCATTCGCCCTCAACATGGCGTATGCCGACTCGACCAACACGGGTGCCCTCGCTGCC GCGTTTTCGGCTGCCGATAGTCTCGGCTTCAAGCTCTTTTTCTCCTTTGACTACGCAGGTAACGGCGACTGGCCT AAAGCCGACGTGATCACCCTCATTCAGCAGTACAGTGCTCACTCGTCCTACTACTTCTATAAGGGACAGGCCTTT GTCTCGACGTTCGAAGGCCCTGGACGCGCCGACGACTGGCCCGAGATCAAGGCGGTCACCGGCTGCTTCTTCATC CCCAGTTGGTCCTCGCTCGGAGCCAAGCCTGCCGTGGCGACGGGGGTCGTGGATGGTCTGTTCAGCTGGGCGGGC TGGCCCTGGGGCCCCCAGGACGCGGACACCTACGTGGACGCCTCGTATCTCCAGTATCTGGAGTCGCTGCCGTAC ATGATGCCGGTCTCGCCGTGGTTCTTCACCAACCTTCCCGGGTACAAAAAAAACTGGATGTGGCGCGGGGACCAC CTGTGGCATGATCGATGGCAGCAGGTCCTATACATTCAACCGGAGTTTGTCGAGATCATCTCGTGGAACGACTAC GGTGAGTCGCACTACATCGGCCCGCTGTACGACCAGGCCATGGAAGCGTTTGAGGTCGGCGAGGCCCCCTTCAAC TACGCGACAGAAATGCCCCACGACGGCTGGCGGGCAACGATCCCCTTCTGGGCCGATATGTATAAGGATGGAACG GCCGAGGTGACTGAGGAGACGATTATCGCCTGGTATCGGCTTACCCCCGGCGCGGCCTGTGACAGCGGCGGCACA AGCGGCAATACGGCCTCCCAGCTGCAGGTTGAGTTCCCCCCGGCGGAGATAGCCCAGGACCGTATCTTCTTCTCC GCAGTGTTGGGCTCGTTCTCGGGCGCCGTAGTGTCGATTGGAGACTGGACCCAGACGGTTGGGTGGTTCGATGTG CCGGATGATGATGTCGGGGTATACTACGGCGATGTAGCCATTGGCGCCCATCGGGGTCCGGTGACTGTGTCTTTG ATGCGTGATAATGTGATTATTGCGACGATTGAGGGCAAGGCCATCTCGTCGACCTGTGAACATGGAGTTCAGAAT TGGAATGCCTGGGTTGGAAGTACGTCTACCGGGGAGACCGTGTCGGCACGACCGACCCGTCTTCTGTCAGAGCAG GTCTGCATGAACGGTACCGGCGCGAATAATTTTGCCGGACTGTGTGGGTTTGCCTGCCGATACGGATACTGTCCT CTGGGCGCCTGTACCTGCACGCGAATGGGCATCGGGTACGAGCCGCCCAACTCCACGGGTGTCCTCGGATACCCG ATTGTGGGCGAAGGCGCGAGTTACAGCGGACTGTGCTCGTTTGACTGCAACCTGGGCTTCTGCCCCGAGAGTGCC TGCGGCACTGTGGAGGTCCCGCTCAGCACGCCGACAGTCTCGCCGTTCCTGCCCCCCGCCTGTGTGTCCGGTTCG GGTGAGGGCAACCTAGCTGGGCTGTGCGACTTTGGCTGTGCCCATGGCTTCTGCCCCATGAACGCGTGCACCTGC ACGGGGCAGGGGGCGCTAAATGTCATGGATCCGACAAGCGAGACCGTTGGGACCGCCGCACCGGGAGTAGACGCT ACCGTCTACGGTCCTCTGTGCGAGTACACTTGTCAACGCGGATACTGTCCCGAAGGCGCCTGTATCGTCGCCGAG GACTCGGACTCGGGCTCGGGGTCCGGGGATGGACCAGGCGATGGCGAGGTCTACATTGCCCCGTCGATCTGGAAT ATGCCCTCGCCGGTCGTGCAATGTGAGCCTCCGTGCTCGCTGATCATGCCTCCGCTGCAGCTGGCCAGCCCTTCT GTAATATCCATCCCTCCCTGGGAGAGCCCCGTCGAGCAGAGTTACTTGACGACCAGAACGACTACCTATGACGAC GGAGTCGTCGAAACATACAATGGCTATGCGGTTGCCACCACCACCATTACCATATCCTTTCCCCCTTCGGTCGTC ACCGAGATCCCCGTATGGGGAGTGACCATCAATGCCAGCCAGGCTCAGCCAACCGAGTTGGAAATGACGAGGAGC ATCACCCTGCCCTATATGGTATGGTCGCTTCCCCCGTTCGATGAAGACCCCAACGCGACAAGCATGACGGGGACG GTCCTTCCGCCCCCATATCCATGGTCCCAGGCCAGCAAGGACTCCGAGCTCAATACCGCGACCACCACCTGGTCG TCCGGCAGTGCGACTCCCACGGCAGATAGGGGTAACCCGGACGTCGGCGTCTCGTGTAAGCCATTCTGCCACCTG TGTCTCTTGTGCCGCCCAGACTGGAGCGGGGGCGGCGGCGGGGGCTCCGACGATGGCAGCAACAGCAACAGCAAC AGCAACAGCAACGAGGACGAGTGCAGCACGCGAGAGGCGGAGATTTGCTCTACCGTGTGTGTGGCCGGGTCCGGG TGCGAGTTCGACTGCTCCACGACCACCGGCTGTTCCGTCACGGCCTCGGCCACCAGCACCGTGGGCACGCCCGCG CCCGCCGTGGCCATCACGCTGGAGCAATGGCCTGAAGCCACCGAGGAGCCCGATGCAGAGGTGACCAGCATCGCT CGGTCGCTCGACTCCCGGCTCTCCGCCCTGTATGGAGACCTCACCGTCCTAACGGGATACGAGGATGGAGCCGCC ACGGCCACGGCCACCAGCACCAGTCGCGCGACCACGACGACCAGCGCCCCCCCGGACCTCCCAACCTCACTTTCC GACCTCCAGTTCGTGATCTTCCTGCGCAAACCCGCCGACTCCCTGGACTCCTGGACCTGGTCCGGCTACCTCCTC CCGACGTACTACGACGAGGAGTACGTGTGCAACAACGCGTACGAGGTTGTCACGAGCACCGATAATATCCCAAAT CAAACGGATGAGGACTCCGACTACGGATACCCGATGTATCTGGGTCCGTTTGCCGCGCAGGATTTGAACTGTGGG TATGTGTCCCAAGGCCTGACGCCGGGCGACGTGCACTGCGGCACTCAGGTACTGACGTGCCTCGAGCTTGATGCG CTGCATGCTCTGGGGTATCCGGGGTATACTTGTGGGAATACGGAGTTTTACATGCAGGCTTGGTGCCCGATGGCA GACTAA
Gene	>Pro_DTO377G3_2|g8186.t1 ATGGTCTCCTTGCTGGCTTGGGCCACGGTGCTGCTGCTCGCCTGCCGAGTGCAGCTGCAAGTACAGGCAGCGGCC GTCTTCGCGCACTTTATGGTTGGCACTCCGCCGTATTCTCTGTCGTCTGTTCTCGCTGACCGTGCAGGTCACAAA CACGGCCAACTATACTTCTAATGACTGGGAGAACGATATTCTTCTGGCGCAGGATGCGCACATCGACGCATTCGC CCTCAACATGGCGTATGCCGACTCGACCAACACGGGTGCCCTCGCTGCCGCGTTTTCGGCTGCCGATAGTCTCGG CTTCAAGCTCTTTTTCTCCTTTGACTACGCAGGTAACGGCGACTGGCCTAAAGCCGACGTGATCACCCTCATTCA GCAGTACAGTGCTCACTCGTCCTACTACTTCTATAAGGGACAGGCCTTTGTCTCGACGTTCGAAGGCCCTGGACG CGCCGACGACTGGCCCGAGATCAAGGCGGTCACCGGCTGCTTCTTCATCCCCAGTTGGTCCTCGCTCGGAGCCAA GCCTGCCGTGGCGACGGGGGTCGTGGATGGTCTGTTCAGCTGGGCGGGCTGGCCCTGGGGCCCCCAGGACGCGGA CACCTACGTGGACGCCTCGTATCTCCAGTATCTGGAGTCGCTGCCGTACATGATGCCGGTCTCGCCGTGGTTCTT CACCAACCTTCCCGGGTACAAAAAAAACTGGATGTGGCGCGGGGACCACCTGTGGCATGATCGATGGCAGCAGGT CCTATACATTCAACCGGAGTTTGTCGAGATCATCTCGTGGAACGACTACGGTGAGTCGCACTACATCGGCCCGCT GTACGACCAGGCCATGGAAGCGTTTGAGGTCGGCGAGGCCCCCTTCAACTACGCGACAGAAATGCCCCACGACGG CTGGCGGGCAACGATCCCCTTCTGGGCCGATATGTATAAGGATGGAACGGCCGAGGTGACTGAGGAGACGATTAT CGCCTGGTATCGGCTTACCCCCGGCGCGGCCTGTGACAGCGGCGGCACAAGCGGCAATACGGCCTCCCAGCTGCA GGTTGAGTTCCCCCCGGCGGAGATAGCCCAGGACCGTATCTTCTTCTCCGCAGTGTTGGGCTCGTTCTCGGGCGC CGTAGTGTCGATTGGAGACTGGACCCAGACGGTTGGGTGGTTCGATGTGCCGGATGATGATGTCGGGGTATACTA CGGCGATGTAGCCATTGGCGCCCATCGGGGTCCGGTGACTGTGTCTTTGATGCGTGATAATGTGATTATTGCGAC GATTGAGGGCAAGGCCATCTCGTCGACCTGTGAACATGGAGTTCAGAATTGGAATGCCTGGGTTGGAAGTACGTC TACCGGGGAGACCGTGTCGGCACGACCGACCCGTCTTCTGTCAGAGCAGGTCTGCATGAACGGTACCGGCGCGAA TAATTTTGCCGGACTGTGTGGGTTTGCCTGCCGATACGGATACTGTCCTCTGGGCGCCTGTACCTGCACGCGAAT GGGCATCGGGTACGAGCCGCCCAACTCCACGGGTGTCCTCGGATACCCGATTGTGGGCGAAGGCGCGAGTTACAG CGGACTGTGCTCGTTTGACTGCAACCTGGGCTTCTGCCCCGAGAGTGCCTGCGGCACTGTGGAGGTCCCGCTCAG CACGCCGACAGTCTCGCCGTTCCTGCCCCCCGCCTGTGTGTCCGGTTCGGGTGAGGGCAACCTAGCTGGGCTGTG CGACTTTGGCTGTGCCCATGGCTTCTGCCCCATGAACGCGTGCACCTGCACGGGGCAGGGGGCGCTAAATGTCAT GGATCCGACAAGCGAGACCGTTGGGACCGCCGCACCGGGAGTAGACGCTACCGTCTACGGTCCTCTGTGCGAGTA CACTTGTCAACGCGGATACTGTCCCGAAGGCGCCTGTATCGTCGCCGAGGACTCGGACTCGGGCTCGGGGTCCGG GGATGGACCAGGCGATGGCGAGGTCTACATTGCCCCGTCGATCTGGAATATGCCCTCGCCGGTCGTGCAATGTGA GCCTCCGTGCTCGCTGATCATGCCTCCGCTGCAGCTGGCCAGCCCTTCTGTAATATCCATCCCTCCCTGGGAGAG CCCCGTCGAGCAGAGTTACTTGACGACCAGAACGACTACCTATGACGACGGAGTCGTCGAAACATACAATGGCTA TGCGGTTGCCACCACCACCATTACCATATCCTTTCCCCCTTGTACGTGTTGAGCCCTCCTCCCCAATCCACCCTC ACTGACAGTCCATCATCAGCGGTCGTCACCGAGATCCCCGTATGGGGAGTGACCATCAATGCCAGCCAGGCTCAG CCAACCGAGTTGGAAATGACGAGGAGCATCACCCTGCCCTATATGGTATGGTCGCTTCCCCCGTTCGATGAAGAC CCCAACGCGACAAGCATGACGGGGACGGTCCTTCCGCCCCCATATCCATGGTCCCAGGCCAGCAAGGACTCCGAG CTCAATACCGCGACCACCACCTGGTCGTCCGGCAGTGCGACTCCCACGGCAGATAGGGGTAACCCGGACGTCGGC GTCTCGTGTAAGCCATTCTGCCACCTGTGTCTCTTGTGCCGCCCAGACTGGAGCGGGGGCGGCGGCGGGGGCTCC GACGATGGCAGCAACAGCAACAGCAACAGCAACAGCAACGAGGACGAGTGCAGCACGCGAGAGGCGGAGATTTGC TCTACCGTGTGTGTGGCCGGGTCCGGGTGCGAGTTCGACTGCTCCACGACCACCGGCTGTTCCGTCACGGCCTCG GCCACCAGCACCGTGGGCACGCCCGCGCCCGCCGTGGCCATCACGCTGGAGCAATGGCCTGAAGCCACCGAGGAG CCCGATGCAGAGGTGACCAGCATCGCTCGGTCGCTCGACTCCCGGCTCTCCGCCCTGTATGGAGACCTCACCGTC CTAACGGGATACGAGGATGGAGCCGCCACGGCCACGGCCACCAGCACCAGTCGCGCGACCACGACGACCAGCGCC CCCCCGGACCTCCCAACCTCACTTTCCGACCTCCAGTTCGTGATCTTCCTGCGCAAACCCGCCGACTCCCTGGAC TCCTGGACCTGGTCCGGCTACCTCCTCCCGACGTACTACGACGAGGAGTACGTGTGCAACAACGCGTACGAGGTT GTCACGAGCACCGATAATATCCCAAATCAAACGGATGAGGACTCCGACTACGGATACCCGATGTATCTGGGTCCG TTTGCCGCGCAGGATTTGAACTGTGGGTATGTGTCCCAAGGCCTGACGCCGGGCGACGTGCACTGCGGCACTCAG GTACTGACGTGCCTCGAGCTTGATGCGCTGCATGCTCTGGGGTATCCGGGGTATACTTGTGGGAATACGGAGTTT TACATGCAGGCTTGGTGCCCGATGGCAGACTAA