© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Pro_DTO377G3_2|g6512.t1 |
| Gene name | |
| Location | scaffold_17:416928..418047 |
| Strand | - |
| Gene length (bp) | 1119 |
| Transcript length (bp) | 945 |
| Coding sequence length (bp) | 945 |
| Protein length (aa) | 315 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF04321 | RmlD_sub_bind | RmlD substrate binding domain | 4.1E-56 | 4 | 294 |
| PF01370 | Epimerase | NAD dependent epimerase/dehydratase family | 2.6E-21 | 5 | 188 |
| PF07993 | NAD_binding_4 | Male sterility protein | 1.2E-08 | 50 | 180 |
| PF01073 | 3Beta_HSD | 3-beta hydroxysteroid dehydrogenase/isomerase family | 4.4E-08 | 42 | 182 |
| PF02719 | Polysacc_synt_2 | Polysaccharide biosynthesis protein | 9.8E-07 | 45 | 113 |
| PF16363 | GDP_Man_Dehyd | GDP-mannose 4,6 dehydratase | 2.7E-07 | 41 | 159 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0006694 | steroid biosynthetic process | Yes |
| GO:0003854 | 3-beta-hydroxy-delta5-steroid dehydrogenase activity | Yes |
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | Yes |
| GO:0009058 | biosynthetic process | No |
| GO:0003824 | catalytic activity | No |
| GO:0008202 | steroid metabolic process | No |
| GO:0033764 | steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | No |
| GO:0008150 | biological_process | No |
| GO:1901360 | organic cyclic compound metabolic process | No |
| GO:0071704 | organic substance metabolic process | No |
| GO:0008152 | metabolic process | No |
| GO:0006629 | lipid metabolic process | No |
| GO:0003674 | molecular_function | No |
| GO:1901362 | organic cyclic compound biosynthetic process | No |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors | No |
| GO:0016491 | oxidoreductase activity | No |
| GO:1901576 | organic substance biosynthetic process | No |
| GO:0016229 | steroid dehydrogenase activity | No |
| GO:0044238 | primary metabolic process | No |
| GO:0008610 | lipid biosynthetic process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 16 | 0.45 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| 3d_25C | Three days - 25 degrees Celsius | 359.74 | 215.50 | 503.98 |
| 5d_25C | Five days - 25 degrees Celsius | 403.32 | 239.54 | 567.10 |
| 7d_25C | Seven days - 25 degrees Celsius | 360.62 | 215.45 | 505.80 |
| 10d_25C | Ten days - 25 degrees Celsius | 428.57 | 254.04 | 603.09 |
| 7d_15C | Seven days - 15 degrees Celsius | 583.92 | 326.23 | 841.62 |
| 7d_30C | Seven days - 30 degrees Celsius | 298.64 | 175.87 | 421.41 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| 3d_25C | 5d_25C | 0.690273 | no |
| 3d_25C | 7d_25C | 0.995147 | no |
| 3d_25C | 7d_15C | 0.022435 | yes |
| 3d_25C | 7d_30C | 0.465617 | no |
| 3d_25C | 10d_25C | 0.499334 | no |
| 5d_25C | 7d_25C | 0.706697 | no |
| 5d_25C | 7d_15C | 0.117342 | no |
| 5d_25C | 7d_30C | 0.193405 | no |
| 5d_25C | 10d_25C | 0.851044 | no |
| 7d_25C | 7d_15C | 0.027506 | yes |
| 7d_25C | 7d_30C | 0.465330 | no |
| 7d_25C | 10d_25C | 0.512781 | no |
| 7d_15C | 7d_30C | 0.002761 | yes |
| 7d_15C | 10d_25C | 0.209139 | no |
| 7d_30C | 10d_25C | 0.104340 | no |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Pro_DTO377G3_2|g6512.t1 MTSNVLVTGASGLLGRSVFNTFQHSGVLVVGQGFTRPAPPTILKADLEKEEDIRMLFDEVKPQIVIHCAANRSPD LCDQHPDQARKVNVDATRALAKECQARGAFLIYISTDYVFPGTEGEAPYEANAETKPPNLYGQLKRDGEVAVLEE TSASGMGVVLRVPVLYGTAKNNSESAINTLIDAVTKATDENAGVKMDDWGIRYPTNTEDVARVCRDIVIKYLREK EKRKDFPHILQFTSEDRMTKYEICQKLAKVMGVAIPGMIPNKQGNDPNSSVVRPYDTHLSTKALTELGIDVRTVD FIAWWRREMGAYRK* |
| Coding | >Pro_DTO377G3_2|g6512.t1 ATGACATCTAACGTTCTTGTTACTGGAGCCAGTGGCTTGCTCGGCCGTTCTGTTTTCAACACCTTCCAGCACTCG GGCGTGTTGGTTGTTGGCCAGGGATTCACCCGCCCTGCCCCGCCTACTATCTTGAAGGCCGACCTGGAGAAGGAG GAGGATATCCGAATGCTGTTCGATGAAGTCAAGCCTCAGATTGTCATCCATTGCGCCGCAAACAGGTCCCCGGAC CTATGTGACCAGCACCCAGATCAAGCCCGCAAGGTCAATGTCGATGCCACTCGAGCACTGGCTAAGGAATGCCAA GCTCGCGGCGCCTTCCTCATCTACATCTCGACCGACTACGTCTTCCCCGGCACCGAAGGCGAGGCACCATACGAG GCCAATGCCGAGACCAAGCCACCGAATCTGTACGGGCAATTGAAGCGTGATGGCGAGGTGGCCGTATTAGAAGAG ACCAGTGCCTCAGGCATGGGCGTGGTGCTGCGCGTGCCGGTGCTGTACGGCACCGCCAAGAATAACTCGGAGAGC GCGATCAACACCCTCATCGACGCAGTAACAAAGGCTACGGATGAAAACGCCGGCGTGAAAATGGACGACTGGGGC ATACGGTACCCAACCAACACCGAGGACGTGGCGCGCGTGTGTCGGGACATTGTCATCAAGTACCTGCGCGAGAAG GAGAAGCGGAAGGACTTCCCTCACATCCTGCAATTTACGTCCGAGGACCGTATGACCAAGTATGAGATTTGTCAG AAGTTGGCCAAGGTGATGGGTGTGGCCATTCCCGGAATGATCCCCAATAAGCAGGGCAATGATCCCAATTCGAGT GTCGTACGGCCTTATGATACACATCTGTCTACCAAGGCATTGACTGAGCTTGGGATTGATGTGCGAACAGTGGAC TTCATTGCGTGGTGGCGCCGGGAAATGGGTGCTTATAGGAAATAA |
| Transcript | >Pro_DTO377G3_2|g6512.t1 ATGACATCTAACGTTCTTGTTACTGGAGCCAGTGGCTTGCTCGGCCGTTCTGTTTTCAACACCTTCCAGCACTCG GGCGTGTTGGTTGTTGGCCAGGGATTCACCCGCCCTGCCCCGCCTACTATCTTGAAGGCCGACCTGGAGAAGGAG GAGGATATCCGAATGCTGTTCGATGAAGTCAAGCCTCAGATTGTCATCCATTGCGCCGCAAACAGGTCCCCGGAC CTATGTGACCAGCACCCAGATCAAGCCCGCAAGGTCAATGTCGATGCCACTCGAGCACTGGCTAAGGAATGCCAA GCTCGCGGCGCCTTCCTCATCTACATCTCGACCGACTACGTCTTCCCCGGCACCGAAGGCGAGGCACCATACGAG GCCAATGCCGAGACCAAGCCACCGAATCTGTACGGGCAATTGAAGCGTGATGGCGAGGTGGCCGTATTAGAAGAG ACCAGTGCCTCAGGCATGGGCGTGGTGCTGCGCGTGCCGGTGCTGTACGGCACCGCCAAGAATAACTCGGAGAGC GCGATCAACACCCTCATCGACGCAGTAACAAAGGCTACGGATGAAAACGCCGGCGTGAAAATGGACGACTGGGGC ATACGGTACCCAACCAACACCGAGGACGTGGCGCGCGTGTGTCGGGACATTGTCATCAAGTACCTGCGCGAGAAG GAGAAGCGGAAGGACTTCCCTCACATCCTGCAATTTACGTCCGAGGACCGTATGACCAAGTATGAGATTTGTCAG AAGTTGGCCAAGGTGATGGGTGTGGCCATTCCCGGAATGATCCCCAATAAGCAGGGCAATGATCCCAATTCGAGT GTCGTACGGCCTTATGATACACATCTGTCTACCAAGGCATTGACTGAGCTTGGGATTGATGTGCGAACAGTGGAC TTCATTGCGTGGTGGCGCCGGGAAATGGGTGCTTATAGGAAATAA |
| Gene | >Pro_DTO377G3_2|g6512.t1 ATGACATCTAACGTTCTTGTTACTGGAGCCAGTGGCTTGCTCGGCCGTTCTGTTTTCAACACCTTCCAGCACTCG GGCGTGTTGGTTGTTGGCCAGGGATTCACCCGCCCTGCCCCGCCTACTATCTTGAAGGCCGACCTGGAGAAGGAG GAGGATATCCGAATGCTGTTCGATGAAGTCAAGTATGTATATTCCATTTAGATCTTGATTATAGACATTAATCAC CTTTGAACTATGCCTAGGCCTCAGATTGTCATCCATTGTAAGCTAGTTAATTGGCTGACTTGATACCCCATCATA GACTGACAGCTCACAGGCGCCGCAAACAGGTCCCCGGACCTATGTGACCAGCACCCAGATCAAGCCCGCAAGGTC AATGTCGATGCCACTCGAGCACTGGCTAAGGAATGCCAAGCTCGCGGCGCCTTCCTCATCTACATCTCGACCGAC TACGTCTTCCCCGGCACCGAAGGCGAGGCACCATACGAGGCCAATGCCGAGACCAAGCCACCGAATCTGTACGGG CAATTGAAGCGTGATGGCGAGGTGGCCGTATTAGAAGAGACCAGTGCCTCAGGCATGGGCGTGGTGCTGCGCGTG CCGGTGCTGTACGGCACCGCCAAGAATAACTCGGAGAGCGCGATCAACACCCTCATCGACGCAGTAACAAAGGCT ACGGATGAAAACGCCGGCGTGAAAATGGACGACTGGGGCATACGGTACCCAACCAACACCGAGGACGTGGCGCGC GTGTGTCGGGACATTGTCATCAAGTACCTGCGCGAGAAGGAGAAGCGGAAGGACTTCCCTCACATCCTGCAATTT ACGTCCGAGGACCGTATGACCAAGTATGAGATTTGTCAGAAGTTGGCCAAGGTGATGGGTGTGGCCATTCCCGGA ATGATCCCCAATAAGCAGGGCAATGATCCCAATTCGAGTGTCGTACGGCCTTATGATACACATCTGTCTACCAAG GCATTGACTGAGCTTGGGATTGATGTGCGAACAGTGGACTTCATTGCGTGGTGGTAAGTTCTCCCCGGGGTGTAC CTATACCTTGTTTGTATGTCTATGCTAACAACCCATAGGCGCCGGGAAATGGGTGCTTATAGGAAATAA |