Fungal Genomics

Agaricus bisporus var bisporus H39 Agaricus bisporus var bisporus H97 Agaricus bisporus var burnettii H119p4 Aspergillus niger CBS 112.32 Aspergillus niger CBS 113.50 Aspergillus niger CBS 115988 Aspergillus niger CBS 115989 Aspergillus niger CBS 118.52 Aspergillus niger CBS 124.48 Aspergillus niger CBS 131.52 Aspergillus niger CBS 133816 Aspergillus niger CBS 147320 Aspergillus niger CBS 147321 Aspergillus niger CBS 147322 Aspergillus niger CBS 147323 Aspergillus niger CBS 147324 Aspergillus niger CBS 147343 Aspergillus niger CBS 147344 Aspergillus niger CBS 147345 Aspergillus niger CBS 147346 Aspergillus niger CBS 147347 Aspergillus niger CBS 147352 Aspergillus niger CBS 147353 Aspergillus niger CBS 147371 Aspergillus niger CBS 147482 Aspergillus niger CBS 630.78 Aspergillus niger CBS 769.97 Ophiocordyceps australis 1348a (Ghana) Ophiocordyceps australis map64 (Brazil) Ophiocordyceps camponoti-floridani Ophiocordyceps camponoti-rufipedis Ophiocordyceps kimflemingae Ophiocordyceps subramaniannii Penicillium roqueforti DTO002I6 Penicillium roqueforti DTO003C3 Penicillium roqueforti DTO003H1 (CBS 147308) Penicillium roqueforti DTO006G1 Penicillium roqueforti DTO006G7 Penicillium roqueforti DTO012A1 Penicillium roqueforti DTO012A2 (CBS 147309) Penicillium roqueforti DTO012A6 (CBS 147310) Penicillium roqueforti DTO012A7 Penicillium roqueforti DTO012A8 Penicillium roqueforti DTO012A9 Penicillium roqueforti DTO013E5 Penicillium roqueforti DTO013F2 Penicillium roqueforti DTO013F5 (CBS 147311) Penicillium roqueforti DTO027I6 Penicillium roqueforti DTO032C6 Penicillium roqueforti DTO039G3 Penicillium roqueforti DTO046C5 Penicillium roqueforti DTO070G2 (CBS 147317) Penicillium roqueforti DTO081F9 (CBS 147318) Penicillium roqueforti DTO101D6 (CBS 147325) Penicillium roqueforti DTO102I9 (CBS 147326) Penicillium roqueforti DTO126G2 (CBS 147330) Penicillium roqueforti DTO127F7 (CBS 147331) Penicillium roqueforti DTO127F9 (CBS 147332) Penicillium roqueforti DTO130C1 (CBS 147333) Penicillium roqueforti DTO163C3 (CBS 147337) Penicillium roqueforti DTO163F5 (CBS 147338) Penicillium roqueforti DTO163G4 (CBS 147339) Penicillium roqueforti DTO265D5 (CBS 147372) Penicillium roqueforti DTO369A1 (CBS 147354) Penicillium roqueforti DTO375B1 (CBS 147355) Penicillium roqueforti DTO377G2 (LCP 96.3914a) Penicillium roqueforti LCP96 04111 Rhodonia placenta FPRL280

General Properties

Protein ID	Pro_DTO377G3_2|g5468.t1
Gene name
Location	scaffold_13:198478..201168
Strand	-
Gene length (bp)	2690
Transcript length (bp)	2628
Coding sequence length (bp)	2628
Protein length (aa)	876

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PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF17681	GCP_N_terminal	Gamma tubulin complex component N-terminal	1.8E-35	236	517
PF04130	GCP_C_terminal	Gamma tubulin complex component C-terminal	1.0E-21	609	852

GO

GO Term	Description	Terminal node
GO:0043015	gamma-tubulin binding	Yes
GO:0005515	protein binding	No
GO:0008092	cytoskeletal protein binding	No
GO:0005488	binding	No
GO:0015631	tubulin binding	No
GO:0003674	molecular_function	No

SignalP

[Help with interpreting these statistics]

SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
No	1 - 14	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Genome-wide gene expression in conidia of Penicillium roqueforti during growth at various temperatures and for various amounts of time. Published in Punt et al., 2020.

Expression values

Label	Description	Expression (RPKM)	Confidence interval (low)	Confidence interval (high)
3d_25C	Three days - 25 degrees Celsius	35.71	21.70	49.72
5d_25C	Five days - 25 degrees Celsius	31.38	18.95	43.81
7d_25C	Seven days - 25 degrees Celsius	29.02	17.32	40.73
10d_25C	Ten days - 25 degrees Celsius	31.14	18.72	43.57
7d_15C	Seven days - 15 degrees Celsius	43.51	25.81	61.21
7d_30C	Seven days - 30 degrees Celsius	25.83	15.23	36.43

Differential expression

Label1	Label2	Q-value	Significant difference
3d_25C	5d_25C	0.640604	no
3d_25C	7d_25C	0.411957	no
3d_25C	7d_15C	0.441793	no
3d_25C	7d_30C	0.153031	no
3d_25C	10d_25C	0.621837	no
5d_25C	7d_25C	0.800616	no
5d_25C	7d_15C	0.150713	no
5d_25C	7d_30C	0.442143	no
5d_25C	10d_25C	0.984308	no
7d_25C	7d_15C	0.067273	no
7d_25C	7d_30C	0.687849	no
7d_25C	10d_25C	0.826229	no
7d_15C	7d_30C	0.014096	yes
7d_15C	10d_25C	0.148853	no
7d_30C	10d_25C	0.473609	no

Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>Pro_DTO377G3_2|g5468.t1 MALAADLSTLIDALITSVAKISPNHKGSARVQSLKRRVQGALRTGSHTRTDQFAVAKQLEGLQEKFQILNRDELA EALHARLAELESDRMPWLPEILSLLLQLSDRPALLSTVKRPKERAKAPEAKETLSWSDLDAAGTAFSDEEIWEQV DFAGDSSDDDFSSVASDVSLPKDRPQSATTVDEGYIIPDDVFVSEEDGDLVASIEKAQFWRAENHHSLPQAEATT SRLITESQLARETIFMLQGLPTSIYVRLDDDFGVDRRYTLAHSSNEALASLLQCFIEIGAKLNAVRYFTKLPQTI PYMQTFCRSLEERLLEFDRVLSQVQCKYLSPGSTVSLIQLLVDVRQHSHELALLADMIANLERNSNDQPMCCLDS LYNLVCMLEALGDESSSQRLAELFFMCFKTYSSSIRLWMETGQVDASDSTFFVRTNRENGDLRTLWHDWFVLDTG HRQQRIPQFLEAGVQKVFTAGKSMVFLRHLNALPDIEAMKFEDFYCQDSSLLLPFPALVESAFEKLVDVDHSLSA SLLRTELDQQCGLWTSLDALQHVYLAKDMSAISIVDAKIFELIDRGRSWDDRFLLTEISRTAFSSISVIDVSRLL VRPASTSLRVSQNDHRTVGILEAISIDYGLPWPIANIITEDAIQAYQRISTFLMQIRRAKYAIVKQRIRDARIIA PDEKDTLVHALHHNMLWVLDLIYGHLTYLVITTTTQSLHKDLSGAQDVDAMIAAHQSYMSSLEAQCLLSEDLSHI HDAIIHFLDLCVHFADLQTAHLLGDEDQYLENPKALRTKKKPEEEFESDDDEDDDDNEAYDHEHTLTVSFRDSTY SQQLKTVNYQFNHLTTFVTDGLKSLVRADGLQSWDILADRLEWRRNWRRL*
Coding	>Pro_DTO377G3_2|g5468.t1 ATGGCTCTAGCGGCTGATCTGAGTACGCTTATCGACGCCTTAATCACTTCTGTCGCTAAGATATCACCAAACCAC AAGGGGTCTGCACGTGTCCAGAGTCTGAAGCGCCGTGTACAAGGAGCGCTCCGAACTGGTTCTCATACGCGCACC GACCAGTTTGCAGTCGCCAAGCAACTCGAGGGACTACAGGAAAAGTTTCAAATCTTGAATAGAGATGAACTTGCT GAAGCTCTTCACGCCCGCCTTGCGGAGCTGGAGAGCGACCGGATGCCCTGGCTTCCAGAGATTCTGAGTCTCTTA TTGCAACTCTCAGATCGCCCCGCTCTTTTGTCAACGGTCAAACGTCCGAAGGAACGGGCCAAGGCACCAGAAGCA AAAGAGACTCTTTCTTGGTCGGACCTTGATGCAGCTGGGACGGCATTCAGCGACGAAGAAATTTGGGAACAAGTA GACTTTGCTGGCGACTCATCAGATGACGACTTTTCATCCGTTGCCAGTGATGTATCTCTTCCCAAGGACAGACCT CAATCCGCCACAACAGTGGATGAAGGGTATATCATTCCAGATGACGTTTTTGTTTCGGAAGAGGATGGCGATCTT GTTGCCTCTATAGAAAAGGCCCAATTTTGGAGAGCCGAGAATCACCATTCGTTGCCCCAAGCAGAAGCAACCACC TCGCGACTTATCACAGAATCCCAGCTTGCTAGGGAGACAATCTTCATGCTTCAGGGCCTTCCCACGTCTATTTAC GTGCGCTTGGACGATGATTTTGGAGTGGATCGAAGATACACACTCGCACATTCATCCAACGAAGCCCTGGCATCG CTTTTGCAGTGTTTCATTGAGATAGGCGCCAAGCTCAACGCTGTACGGTATTTCACCAAGCTTCCACAGACCATC CCATATATGCAAACGTTTTGCCGGAGCTTAGAAGAGCGTCTCCTTGAATTTGATAGAGTCTTATCTCAAGTTCAG TGCAAATACCTTTCACCCGGTTCAACTGTCAGTCTCATCCAGCTTCTTGTTGATGTTCGTCAGCACTCTCATGAG CTAGCATTGCTCGCAGACATGATCGCCAATTTAGAAAGAAACTCAAATGATCAACCAATGTGCTGCCTTGATTCC TTATACAACTTAGTATGCATGCTGGAAGCCCTTGGCGACGAAAGTTCGTCTCAGCGCCTGGCGGAGCTATTTTTT ATGTGTTTCAAAACATATTCAAGCTCGATCCGACTCTGGATGGAAACAGGTCAGGTCGATGCTTCGGACAGCACT TTCTTCGTGCGAACAAATCGTGAGAATGGGGATCTGCGGACTCTTTGGCATGACTGGTTTGTGCTTGATACGGGC CACAGGCAGCAGAGGATCCCCCAGTTTCTCGAGGCCGGTGTGCAGAAAGTCTTCACTGCAGGAAAGAGCATGGTA TTTCTCCGCCATCTAAATGCGCTACCAGACATTGAAGCCATGAAATTTGAGGACTTCTATTGCCAGGATTCTTCA TTATTACTTCCTTTCCCCGCATTAGTAGAGTCTGCTTTTGAAAAACTCGTGGATGTGGACCATTCACTCAGTGCT AGCTTATTACGAACTGAACTCGACCAACAATGCGGACTATGGACCTCCCTTGATGCTCTTCAACATGTCTACCTT GCCAAAGACATGAGCGCCATCAGCATTGTTGACGCAAAAATCTTTGAGTTGATCGATCGGGGTCGTTCCTGGGAC GACAGATTCCTGCTCACTGAGATATCAAGGACAGCTTTCAGTTCAATATCCGTCATTGACGTATCAAGACTCCTT GTGCGGCCTGCTAGTACTTCATTGCGAGTTTCTCAAAATGATCATCGGACCGTGGGAATTCTAGAAGCAATCTCA ATTGATTATGGCCTCCCGTGGCCTATTGCGAACATAATCACCGAGGACGCAATACAGGCATACCAACGAATCTCG ACCTTCCTGATGCAGATCAGGCGCGCTAAATATGCCATTGTGAAACAGCGTATTCGCGACGCCCGTATCATCGCA CCTGACGAAAAGGACACATTGGTACACGCTCTACATCATAACATGCTCTGGGTCCTCGATCTCATCTACGGCCAT CTGACCTATCTCGTAATAACCACCACAACCCAATCTCTACACAAAGATCTATCTGGCGCACAAGATGTCGATGCC ATGATAGCTGCCCACCAATCCTACATGTCCTCTTTGGAAGCCCAGTGTCTTCTTTCTGAAGATTTATCACATATC CACGATGCAATCATCCATTTCTTAGATCTGTGTGTTCATTTCGCAGATCTACAAACCGCGCACTTGCTCGGCGAT GAAGACCAATATCTGGAGAATCCGAAGGCGCTGCGCACAAAGAAAAAACCCGAAGAAGAATTCGAGTCTGATGAT GATGAAGATGATGATGATAACGAGGCCTACGATCACGAACACACACTCACAGTCTCATTTCGTGACTCGACCTAT AGCCAGCAGCTGAAAACGGTCAATTACCAGTTCAACCATCTAACGACCTTTGTCACGGATGGCTTGAAAAGTTTA GTTCGTGCAGATGGGCTGCAAAGTTGGGATATTCTTGCCGACAGGCTCGAATGGCGGCGAAATTGGCGTAGGCTT TGA
Transcript	>Pro_DTO377G3_2|g5468.t1 ATGGCTCTAGCGGCTGATCTGAGTACGCTTATCGACGCCTTAATCACTTCTGTCGCTAAGATATCACCAAACCAC AAGGGGTCTGCACGTGTCCAGAGTCTGAAGCGCCGTGTACAAGGAGCGCTCCGAACTGGTTCTCATACGCGCACC GACCAGTTTGCAGTCGCCAAGCAACTCGAGGGACTACAGGAAAAGTTTCAAATCTTGAATAGAGATGAACTTGCT GAAGCTCTTCACGCCCGCCTTGCGGAGCTGGAGAGCGACCGGATGCCCTGGCTTCCAGAGATTCTGAGTCTCTTA TTGCAACTCTCAGATCGCCCCGCTCTTTTGTCAACGGTCAAACGTCCGAAGGAACGGGCCAAGGCACCAGAAGCA AAAGAGACTCTTTCTTGGTCGGACCTTGATGCAGCTGGGACGGCATTCAGCGACGAAGAAATTTGGGAACAAGTA GACTTTGCTGGCGACTCATCAGATGACGACTTTTCATCCGTTGCCAGTGATGTATCTCTTCCCAAGGACAGACCT CAATCCGCCACAACAGTGGATGAAGGGTATATCATTCCAGATGACGTTTTTGTTTCGGAAGAGGATGGCGATCTT GTTGCCTCTATAGAAAAGGCCCAATTTTGGAGAGCCGAGAATCACCATTCGTTGCCCCAAGCAGAAGCAACCACC TCGCGACTTATCACAGAATCCCAGCTTGCTAGGGAGACAATCTTCATGCTTCAGGGCCTTCCCACGTCTATTTAC GTGCGCTTGGACGATGATTTTGGAGTGGATCGAAGATACACACTCGCACATTCATCCAACGAAGCCCTGGCATCG CTTTTGCAGTGTTTCATTGAGATAGGCGCCAAGCTCAACGCTGTACGGTATTTCACCAAGCTTCCACAGACCATC CCATATATGCAAACGTTTTGCCGGAGCTTAGAAGAGCGTCTCCTTGAATTTGATAGAGTCTTATCTCAAGTTCAG TGCAAATACCTTTCACCCGGTTCAACTGTCAGTCTCATCCAGCTTCTTGTTGATGTTCGTCAGCACTCTCATGAG CTAGCATTGCTCGCAGACATGATCGCCAATTTAGAAAGAAACTCAAATGATCAACCAATGTGCTGCCTTGATTCC TTATACAACTTAGTATGCATGCTGGAAGCCCTTGGCGACGAAAGTTCGTCTCAGCGCCTGGCGGAGCTATTTTTT ATGTGTTTCAAAACATATTCAAGCTCGATCCGACTCTGGATGGAAACAGGTCAGGTCGATGCTTCGGACAGCACT TTCTTCGTGCGAACAAATCGTGAGAATGGGGATCTGCGGACTCTTTGGCATGACTGGTTTGTGCTTGATACGGGC CACAGGCAGCAGAGGATCCCCCAGTTTCTCGAGGCCGGTGTGCAGAAAGTCTTCACTGCAGGAAAGAGCATGGTA TTTCTCCGCCATCTAAATGCGCTACCAGACATTGAAGCCATGAAATTTGAGGACTTCTATTGCCAGGATTCTTCA TTATTACTTCCTTTCCCCGCATTAGTAGAGTCTGCTTTTGAAAAACTCGTGGATGTGGACCATTCACTCAGTGCT AGCTTATTACGAACTGAACTCGACCAACAATGCGGACTATGGACCTCCCTTGATGCTCTTCAACATGTCTACCTT GCCAAAGACATGAGCGCCATCAGCATTGTTGACGCAAAAATCTTTGAGTTGATCGATCGGGGTCGTTCCTGGGAC GACAGATTCCTGCTCACTGAGATATCAAGGACAGCTTTCAGTTCAATATCCGTCATTGACGTATCAAGACTCCTT GTGCGGCCTGCTAGTACTTCATTGCGAGTTTCTCAAAATGATCATCGGACCGTGGGAATTCTAGAAGCAATCTCA ATTGATTATGGCCTCCCGTGGCCTATTGCGAACATAATCACCGAGGACGCAATACAGGCATACCAACGAATCTCG ACCTTCCTGATGCAGATCAGGCGCGCTAAATATGCCATTGTGAAACAGCGTATTCGCGACGCCCGTATCATCGCA CCTGACGAAAAGGACACATTGGTACACGCTCTACATCATAACATGCTCTGGGTCCTCGATCTCATCTACGGCCAT CTGACCTATCTCGTAATAACCACCACAACCCAATCTCTACACAAAGATCTATCTGGCGCACAAGATGTCGATGCC ATGATAGCTGCCCACCAATCCTACATGTCCTCTTTGGAAGCCCAGTGTCTTCTTTCTGAAGATTTATCACATATC CACGATGCAATCATCCATTTCTTAGATCTGTGTGTTCATTTCGCAGATCTACAAACCGCGCACTTGCTCGGCGAT GAAGACCAATATCTGGAGAATCCGAAGGCGCTGCGCACAAAGAAAAAACCCGAAGAAGAATTCGAGTCTGATGAT GATGAAGATGATGATGATAACGAGGCCTACGATCACGAACACACACTCACAGTCTCATTTCGTGACTCGACCTAT AGCCAGCAGCTGAAAACGGTCAATTACCAGTTCAACCATCTAACGACCTTTGTCACGGATGGCTTGAAAAGTTTA GTTCGTGCAGATGGGCTGCAAAGTTGGGATATTCTTGCCGACAGGCTCGAATGGCGGCGAAATTGGCGTAGGCTT TGA
Gene	>Pro_DTO377G3_2|g5468.t1 ATGGCTCTAGCGGCTGATCTGAGTACGCTTATCGACGCCTTAATCACTTCTGTCGCTAAGATATCACCAAACCAC AAGGTAATATATTGAATTTCCATCAATCTCATATCGTCACACCCGGTGTACTCATATCTAAGTAGGGGTCTGCAC GTGTCCAGAGTCTGAAGCGCCGTGTACAAGGAGCGCTCCGAACTGGTTCTCATACGCGCACCGACCAGTTTGCAG TCGCCAAGCAACTCGAGGGACTACAGGAAAAGTTTCAAATCTTGAATAGAGATGAACTTGCTGAAGCTCTTCACG CCCGCCTTGCGGAGCTGGAGAGCGACCGGATGCCCTGGCTTCCAGAGATTCTGAGTCTCTTATTGCAACTCTCAG ATCGCCCCGCTCTTTTGTCAACGGTCAAACGTCCGAAGGAACGGGCCAAGGCACCAGAAGCAAAAGAGACTCTTT CTTGGTCGGACCTTGATGCAGCTGGGACGGCATTCAGCGACGAAGAAATTTGGGAACAAGTAGACTTTGCTGGCG ACTCATCAGATGACGACTTTTCATCCGTTGCCAGTGATGTATCTCTTCCCAAGGACAGACCTCAATCCGCCACAA CAGTGGATGAAGGGTATATCATTCCAGATGACGTTTTTGTTTCGGAAGAGGATGGCGATCTTGTTGCCTCTATAG AAAAGGCCCAATTTTGGAGAGCCGAGAATCACCATTCGTTGCCCCAAGCAGAAGCAACCACCTCGCGACTTATCA CAGAATCCCAGCTTGCTAGGGAGACAATCTTCATGCTTCAGGGCCTTCCCACGTCTATTTACGTGCGCTTGGACG ATGATTTTGGAGTGGATCGAAGATACACACTCGCACATTCATCCAACGAAGCCCTGGCATCGCTTTTGCAGTGTT TCATTGAGATAGGCGCCAAGCTCAACGCTGTACGGTATTTCACCAAGCTTCCACAGACCATCCCATATATGCAAA CGTTTTGCCGGAGCTTAGAAGAGCGTCTCCTTGAATTTGATAGAGTCTTATCTCAAGTTCAGTGCAAATACCTTT CACCCGGTTCAACTGTCAGTCTCATCCAGCTTCTTGTTGATGTTCGTCAGCACTCTCATGAGCTAGCATTGCTCG CAGACATGATCGCCAATTTAGAAAGAAACTCAAATGATCAACCAATGTGCTGCCTTGATTCCTTATACAACTTAG TATGCATGCTGGAAGCCCTTGGCGACGAAAGTTCGTCTCAGCGCCTGGCGGAGCTATTTTTTATGTGTTTCAAAA CATATTCAAGCTCGATCCGACTCTGGATGGAAACAGGTCAGGTCGATGCTTCGGACAGCACTTTCTTCGTGCGAA CAAATCGTGAGAATGGGGATCTGCGGACTCTTTGGCATGACTGGTTTGTGCTTGATACGGGCCACAGGCAGCAGA GGATCCCCCAGTTTCTCGAGGCCGGTGTGCAGAAAGTCTTCACTGCAGGAAAGAGCATGGTATTTCTCCGCCATC TAAATGCGCTACCAGACATTGAAGCCATGAAATTTGAGGACTTCTATTGCCAGGATTCTTCATTATTACTTCCTT TCCCCGCATTAGTAGAGTCTGCTTTTGAAAAACTCGTGGATGTGGACCATTCACTCAGTGCTAGCTTATTACGAA CTGAACTCGACCAACAATGCGGACTATGGACCTCCCTTGATGCTCTTCAACATGTCTACCTTGCCAAAGACATGA GCGCCATCAGCATTGTTGACGCAAAAATCTTTGAGTTGATCGATCGGGGTCGTTCCTGGGACGACAGATTCCTGC TCACTGAGATATCAAGGACAGCTTTCAGTTCAATATCCGTCATTGACGTATCAAGACTCCTTGTGCGGCCTGCTA GTACTTCATTGCGAGTTTCTCAAAATGATCATCGGACCGTGGGAATTCTAGAAGCAATCTCAATTGATTATGGCC TCCCGTGGCCTATTGCGAACATAATCACCGAGGACGCAATACAGGCATACCAACGAATCTCGACCTTCCTGATGC AGATCAGGCGCGCTAAATATGCCATTGTGAAACAGCGTATTCGCGACGCCCGTATCATCGCACCTGACGAAAAGG ACACATTGGTACACGCTCTACATCATAACATGCTCTGGGTCCTCGATCTCATCTACGGCCATCTGACCTATCTCG TAATAACCACCACAACCCAATCTCTACACAAAGATCTATCTGGCGCACAAGATGTCGATGCCATGATAGCTGCCC ACCAATCCTACATGTCCTCTTTGGAAGCCCAGTGTCTTCTTTCTGAAGATTTATCACATATCCACGATGCAATCA TCCATTTCTTAGATCTGTGTGTTCATTTCGCAGATCTACAAACCGCGCACTTGCTCGGCGATGAAGACCAATATC TGGAGAATCCGAAGGCGCTGCGCACAAAGAAAAAACCCGAAGAAGAATTCGAGTCTGATGATGATGAAGATGATG ATGATAACGAGGCCTACGATCACGAACACACACTCACAGTCTCATTTCGTGACTCGACCTATAGCCAGCAGCTGA AAACGGTCAATTACCAGTTCAACCATCTAACGACCTTTGTCACGGATGGCTTGAAAAGTTTAGTTCGTGCAGATG GGCTGCAAAGTTGGGATATTCTTGCCGACAGGCTCGAATGGCGGCGAAATTGGCGTAGGCTTTGA