© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Pro_DTO377G3_2|g3780.t1 |
| Gene name | |
| Location | scaffold_06:750794..751918 |
| Strand | + |
| Gene length (bp) | 1124 |
| Transcript length (bp) | 939 |
| Coding sequence length (bp) | 939 |
| Protein length (aa) | 313 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF02544 | Steroid_dh | 3-oxo-5-alpha-steroid 4-dehydrogenase | 6.1E-31 | 160 | 310 |
| PF06966 | DUF1295 | Protein of unknown function (DUF1295) | 4.4E-06 | 136 | 278 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0006629 | lipid metabolic process | Yes |
| GO:0016627 | oxidoreductase activity, acting on the CH-CH group of donors | Yes |
| GO:0008150 | biological_process | No |
| GO:0016491 | oxidoreductase activity | No |
| GO:0044238 | primary metabolic process | No |
| GO:0008152 | metabolic process | No |
| GO:0003674 | molecular_function | No |
| GO:0003824 | catalytic activity | No |
| GO:0071704 | organic substance metabolic process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 69 | 0.45 |
| Domain # | Start | End | Length |
|---|---|---|---|
| 1 | 89 | 111 | 22 |
| 2 | 169 | 186 | 17 |
| 3 | 201 | 223 | 22 |
| 4 | 236 | 258 | 22 |
| 5 | 268 | 286 | 18 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| 3d_25C | Three days - 25 degrees Celsius | 125.45 | 76.43 | 174.48 |
| 5d_25C | Five days - 25 degrees Celsius | 132.57 | 80.68 | 184.45 |
| 7d_25C | Seven days - 25 degrees Celsius | 128.27 | 78.11 | 178.44 |
| 10d_25C | Ten days - 25 degrees Celsius | 159.13 | 97.74 | 220.52 |
| 7d_15C | Seven days - 15 degrees Celsius | 151.52 | 93.05 | 209.99 |
| 7d_30C | Seven days - 30 degrees Celsius | 131.79 | 80.06 | 183.52 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| 3d_25C | 5d_25C | 0.864793 | no |
| 3d_25C | 7d_25C | 0.948836 | no |
| 3d_25C | 7d_15C | 0.447496 | no |
| 3d_25C | 7d_30C | 0.881474 | no |
| 3d_25C | 10d_25C | 0.315640 | no |
| 5d_25C | 7d_25C | 0.922095 | no |
| 5d_25C | 7d_15C | 0.615978 | no |
| 5d_25C | 7d_30C | 0.986540 | no |
| 5d_25C | 10d_25C | 0.464072 | no |
| 7d_25C | 7d_15C | 0.520211 | no |
| 7d_25C | 7d_30C | 0.938589 | no |
| 7d_25C | 10d_25C | 0.375153 | no |
| 7d_15C | 7d_30C | 0.599494 | no |
| 7d_15C | 10d_25C | 0.876141 | no |
| 7d_30C | 10d_25C | 0.456032 | no |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Pro_DTO377G3_2|g3780.t1 MAADKITLVVQPRGKPIRKLPKEIDISLDASSEELHVALSAASGCSIHRMRITKGSDHSVVPNSNNITIEDTGLL NSSVIYVKDLGPQIAWRTVFIIEYLGPLLIPALFLFPLRPLLYFTFDKPLPSPSNLQLLVCALLSVHFLKREFET IFVHRFSSATMPARNIVKNSAHYWVLAGFNIAYWVFRPDATAASSTPNQALVYAGLALFVFGELANLNAHYILRN LRRPGTTERGIPSGFGFSVVTCPNYFFEILAWLGIFLVSQLNWSVLIFVAVGGLQMWSWAWKKEYRYRKEFGDKY KKKRSVIFPGLA* |
| Coding | >Pro_DTO377G3_2|g3780.t1 ATGGCGGCTGATAAAATCACACTCGTTGTTCAGCCAAGGGGCAAGCCCATCCGCAAGCTCCCCAAGGAAATCGAT ATCTCTCTCGATGCCTCTAGTGAAGAATTGCACGTTGCGCTCTCCGCAGCATCCGGTTGCTCAATTCACCGGATG AGAATCACCAAGGGTAGCGACCACAGCGTGGTCCCGAACTCGAACAACATCACCATTGAGGACACCGGCCTGCTA AACTCAAGCGTGATCTACGTCAAGGATCTCGGACCCCAAATTGCCTGGCGCACCGTTTTCATCATCGAGTACCTC GGCCCACTCCTGATCCCCGCGCTCTTCCTCTTCCCACTCCGACCCCTCCTGTACTTCACCTTCGACAAGCCCCTC CCCTCGCCCTCAAACCTCCAGCTCCTAGTCTGCGCACTGCTGAGCGTGCACTTCCTGAAGCGCGAGTTCGAGACA ATCTTTGTGCACCGTTTCAGCAGTGCCACCATGCCCGCCCGCAACATCGTCAAGAACAGCGCCCACTACTGGGTT CTGGCCGGTTTCAACATCGCCTACTGGGTGTTCCGTCCGGACGCGACCGCCGCCAGCAGTACCCCCAATCAGGCT CTTGTGTACGCCGGACTGGCCCTGTTTGTCTTCGGTGAGCTGGCGAATCTGAATGCGCACTACATCCTGCGTAAT CTCCGCCGGCCCGGTACCACTGAGCGAGGCATTCCGTCTGGGTTCGGCTTCAGTGTCGTTACCTGTCCCAACTAC TTCTTTGAGATTCTGGCTTGGTTGGGTATTTTCCTTGTCAGCCAGTTGAACTGGAGTGTGCTGATCTTCGTTGCT GTGGGTGGGTTGCAGATGTGGAGCTGGGCTTGGAAGAAGGAGTATCGCTACCGCAAGGAGTTTGGTGACAAGTAT AAGAAGAAGCGCTCTGTCATCTTCCCCGGCCTTGCTTAA |
| Transcript | >Pro_DTO377G3_2|g3780.t1 ATGGCGGCTGATAAAATCACACTCGTTGTTCAGCCAAGGGGCAAGCCCATCCGCAAGCTCCCCAAGGAAATCGAT ATCTCTCTCGATGCCTCTAGTGAAGAATTGCACGTTGCGCTCTCCGCAGCATCCGGTTGCTCAATTCACCGGATG AGAATCACCAAGGGTAGCGACCACAGCGTGGTCCCGAACTCGAACAACATCACCATTGAGGACACCGGCCTGCTA AACTCAAGCGTGATCTACGTCAAGGATCTCGGACCCCAAATTGCCTGGCGCACCGTTTTCATCATCGAGTACCTC GGCCCACTCCTGATCCCCGCGCTCTTCCTCTTCCCACTCCGACCCCTCCTGTACTTCACCTTCGACAAGCCCCTC CCCTCGCCCTCAAACCTCCAGCTCCTAGTCTGCGCACTGCTGAGCGTGCACTTCCTGAAGCGCGAGTTCGAGACA ATCTTTGTGCACCGTTTCAGCAGTGCCACCATGCCCGCCCGCAACATCGTCAAGAACAGCGCCCACTACTGGGTT CTGGCCGGTTTCAACATCGCCTACTGGGTGTTCCGTCCGGACGCGACCGCCGCCAGCAGTACCCCCAATCAGGCT CTTGTGTACGCCGGACTGGCCCTGTTTGTCTTCGGTGAGCTGGCGAATCTGAATGCGCACTACATCCTGCGTAAT CTCCGCCGGCCCGGTACCACTGAGCGAGGCATTCCGTCTGGGTTCGGCTTCAGTGTCGTTACCTGTCCCAACTAC TTCTTTGAGATTCTGGCTTGGTTGGGTATTTTCCTTGTCAGCCAGTTGAACTGGAGTGTGCTGATCTTCGTTGCT GTGGGTGGGTTGCAGATGTGGAGCTGGGCTTGGAAGAAGGAGTATCGCTACCGCAAGGAGTTTGGTGACAAGTAT AAGAAGAAGCGCTCTGTCATCTTCCCCGGCCTTGCTTAA |
| Gene | >Pro_DTO377G3_2|g3780.t1 ATGGCGGCTGATAAAATCACACTCGTTGTTCAGCCAAGGGGTATGCATACATTACTCTTTTACTCGAAGCTCTCG GTCACAGAGAGAGTAGCTTAAAAGCATCGCTAATCTAAAATCAGGCAAGCCCATCCGCAAGCTCCCCAAGGAAAT CGATATCTCTCTCGATGCCTCTAGTGAAGAATTGCACGTTGCGCTCTCCGCAGCATCCGGTTGCTCAATTCACCG GATGAGAATCACCAAGGGTAGCGACCACAGCGTGGTCCCGAACTCGAACAACATCACCATTGAGGACACCGGCCT GCTAAACTCAAGCGTGATCTACGTCAAGGATCTCGGTGCGTGTCTATACCCCATATCATGGACAATGCGAATTCA GCCCTAGGACCAGCGCACTCTTCCCCACGCACATAGAAGCTAACATATAGTTCTTAAATCAAACAGGACCCCAAA TTGCCTGGCGCACCGTTTTCATCATCGAGTACCTCGGCCCACTCCTGATCCCCGCGCTCTTCCTCTTCCCACTCC GACCCCTCCTGTACTTCACCTTCGACAAGCCCCTCCCCTCGCCCTCAAACCTCCAGCTCCTAGTCTGCGCACTGC TGAGCGTGCACTTCCTGAAGCGCGAGTTCGAGACAATCTTTGTGCACCGTTTCAGCAGTGCCACCATGCCCGCCC GCAACATCGTCAAGAACAGCGCCCACTACTGGGTTCTGGCCGGTTTCAACATCGCCTACTGGGTGTTCCGTCCGG ACGCGACCGCCGCCAGCAGTACCCCCAATCAGGCTCTTGTGTACGCCGGACTGGCCCTGTTTGTCTTCGGTGAGC TGGCGAATCTGAATGCGCACTACATCCTGCGTAATCTCCGCCGGCCCGGTACCACTGAGCGAGGCATTCCGTCTG GGTTCGGCTTCAGTGTCGTTACCTGTCCCAACTACTTCTTTGAGATTCTGGCTTGGTTGGGTATTTTCCTTGTCA GCCAGTTGAACTGGAGTGTGCTGATCTTCGTTGCTGTGGGTGGGTTGCAGATGTGGAGCTGGGCTTGGAAGAAGG AGTATCGCTACCGCAAGGAGTTTGGTGACAAGTATAAGAAGAAGCGCTCTGTCATCTTCCCCGGCCTTGCTTAA |