© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Pro_DTO377G3_2|g2749.t1 |
| Gene name | |
| Location | scaffold_03:855834..856794 |
| Strand | - |
| Gene length (bp) | 960 |
| Transcript length (bp) | 960 |
| Coding sequence length (bp) | 960 |
| Protein length (aa) | 320 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF01370 | Epimerase | NAD dependent epimerase/dehydratase family | 6.4E-21 | 3 | 203 |
| PF16363 | GDP_Man_Dehyd | GDP-mannose 4,6 dehydratase | 3.5E-13 | 4 | 159 |
| PF01073 | 3Beta_HSD | 3-beta hydroxysteroid dehydrogenase/isomerase family | 8.2E-12 | 4 | 177 |
| PF04321 | RmlD_sub_bind | RmlD substrate binding domain | 1.5E-06 | 1 | 161 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0006694 | steroid biosynthetic process | Yes |
| GO:0003854 | 3-beta-hydroxy-delta5-steroid dehydrogenase activity | Yes |
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | Yes |
| GO:0009058 | biosynthetic process | No |
| GO:0003824 | catalytic activity | No |
| GO:0008202 | steroid metabolic process | No |
| GO:0033764 | steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | No |
| GO:0008150 | biological_process | No |
| GO:1901360 | organic cyclic compound metabolic process | No |
| GO:0071704 | organic substance metabolic process | No |
| GO:0008152 | metabolic process | No |
| GO:0006629 | lipid metabolic process | No |
| GO:0003674 | molecular_function | No |
| GO:1901362 | organic cyclic compound biosynthetic process | No |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors | No |
| GO:0016491 | oxidoreductase activity | No |
| GO:1901576 | organic substance biosynthetic process | No |
| GO:0016229 | steroid dehydrogenase activity | No |
| GO:0044238 | primary metabolic process | No |
| GO:0008610 | lipid biosynthetic process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 18 | 0.45 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| 3d_25C | Three days - 25 degrees Celsius | 341.59 | 205.67 | 477.52 |
| 5d_25C | Five days - 25 degrees Celsius | 259.92 | 158.07 | 361.77 |
| 7d_25C | Seven days - 25 degrees Celsius | 176.34 | 106.11 | 246.58 |
| 10d_25C | Ten days - 25 degrees Celsius | 139.88 | 85.67 | 194.08 |
| 7d_15C | Seven days - 15 degrees Celsius | 319.77 | 186.80 | 452.74 |
| 7d_30C | Seven days - 30 degrees Celsius | 149.96 | 88.79 | 211.13 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| 3d_25C | 5d_25C | 0.235261 | no |
| 3d_25C | 7d_25C | 0.001278 | yes |
| 3d_25C | 7d_15C | 0.836593 | no |
| 3d_25C | 7d_30C | 0.000700 | yes |
| 3d_25C | 10d_25C | 0.000700 | yes |
| 5d_25C | 7d_25C | 0.072335 | no |
| 5d_25C | 7d_15C | 0.409805 | no |
| 5d_25C | 7d_30C | 0.009446 | yes |
| 5d_25C | 10d_25C | 0.000700 | yes |
| 7d_25C | 7d_15C | 0.003197 | yes |
| 7d_25C | 7d_30C | 0.545116 | no |
| 7d_25C | 10d_25C | 0.323588 | no |
| 7d_15C | 7d_30C | 0.000700 | yes |
| 7d_15C | 10d_25C | 0.000700 | yes |
| 7d_30C | 10d_25C | 0.819516 | no |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Pro_DTO377G3_2|g2749.t1 MHVLITGAAGFIGQLLAKELLNDPAYRVTLTDIHQPPIPAGVRYPQSATAIKVDLLTGAKDIVDSSLDAVYAFHG IMSSGSEDNFDLGMSVNVDATRNLLEALRHTCPGVRFIYSSSQAVYGRPLPEIVTDSVIPTPESSYGAEKIVCET LVNEYTRRKFITGFILRFPTISVRPGAPTAAASSFLSGMIREPLDGQQCVIPIKDRQFKSWLCSPKTLVENLLLT LRLPADSVPPHIRQINVPGICVTVQGMMDALEAVGGADKLALLTEKEDPALVPILKSWPTQFDNTQAISLGFKRD ESFEQTVRDYKQSLTQYRS* |
| Coding | >Pro_DTO377G3_2|g2749.t1 ATGCATGTCCTCATCACTGGCGCTGCCGGCTTCATCGGCCAATTGCTTGCGAAAGAGCTGTTGAATGACCCAGCC TATCGCGTCACACTGACCGATATACATCAACCTCCGATCCCCGCGGGCGTTCGCTATCCTCAATCCGCAACGGCA ATCAAGGTCGATCTCTTGACAGGCGCCAAGGACATTGTCGATTCTTCCTTGGACGCCGTCTATGCCTTTCACGGC ATCATGTCGTCCGGCTCCGAGGACAACTTCGATCTAGGCATGAGCGTCAACGTCGATGCAACCCGCAACCTACTC GAGGCGCTGCGACACACCTGTCCCGGAGTCCGATTCATCTATTCGTCCAGTCAGGCCGTATATGGCCGACCGTTG CCAGAGATTGTGACTGACAGCGTAATTCCGACCCCAGAGTCCTCTTACGGTGCCGAGAAGATCGTTTGCGAAACA TTGGTGAACGAATACACTCGCCGTAAATTCATCACCGGGTTTATCCTGCGGTTTCCTACCATCTCCGTCCGACCG GGTGCACCCACTGCTGCAGCTTCGTCCTTCCTTTCCGGCATGATCCGGGAACCTCTTGACGGCCAGCAATGTGTG ATCCCCATTAAAGACCGACAGTTCAAGTCGTGGCTATGCTCGCCCAAAACCCTTGTGGAAAATCTCCTTCTCACT CTCCGTCTTCCTGCGGACTCCGTTCCCCCGCACATTCGCCAGATTAATGTGCCTGGAATCTGCGTCACAGTCCAG GGCATGATGGATGCATTGGAAGCCGTGGGCGGTGCAGATAAACTCGCCCTTCTAACAGAGAAGGAGGACCCTGCG CTGGTTCCAATCTTGAAGTCATGGCCGACGCAATTTGATAACACTCAAGCCATCTCCTTGGGGTTCAAACGCGAC GAGTCTTTCGAGCAAACCGTGCGGGATTATAAACAGTCATTGACACAATATAGATCTTGA |
| Transcript | >Pro_DTO377G3_2|g2749.t1 ATGCATGTCCTCATCACTGGCGCTGCCGGCTTCATCGGCCAATTGCTTGCGAAAGAGCTGTTGAATGACCCAGCC TATCGCGTCACACTGACCGATATACATCAACCTCCGATCCCCGCGGGCGTTCGCTATCCTCAATCCGCAACGGCA ATCAAGGTCGATCTCTTGACAGGCGCCAAGGACATTGTCGATTCTTCCTTGGACGCCGTCTATGCCTTTCACGGC ATCATGTCGTCCGGCTCCGAGGACAACTTCGATCTAGGCATGAGCGTCAACGTCGATGCAACCCGCAACCTACTC GAGGCGCTGCGACACACCTGTCCCGGAGTCCGATTCATCTATTCGTCCAGTCAGGCCGTATATGGCCGACCGTTG CCAGAGATTGTGACTGACAGCGTAATTCCGACCCCAGAGTCCTCTTACGGTGCCGAGAAGATCGTTTGCGAAACA TTGGTGAACGAATACACTCGCCGTAAATTCATCACCGGGTTTATCCTGCGGTTTCCTACCATCTCCGTCCGACCG GGTGCACCCACTGCTGCAGCTTCGTCCTTCCTTTCCGGCATGATCCGGGAACCTCTTGACGGCCAGCAATGTGTG ATCCCCATTAAAGACCGACAGTTCAAGTCGTGGCTATGCTCGCCCAAAACCCTTGTGGAAAATCTCCTTCTCACT CTCCGTCTTCCTGCGGACTCCGTTCCCCCGCACATTCGCCAGATTAATGTGCCTGGAATCTGCGTCACAGTCCAG GGCATGATGGATGCATTGGAAGCCGTGGGCGGTGCAGATAAACTCGCCCTTCTAACAGAGAAGGAGGACCCTGCG CTGGTTCCAATCTTGAAGTCATGGCCGACGCAATTTGATAACACTCAAGCCATCTCCTTGGGGTTCAAACGCGAC GAGTCTTTCGAGCAAACCGTGCGGGATTATAAACAGTCATTGACACAATATAGATCTTGA |
| Gene | >Pro_DTO377G3_2|g2749.t1 ATGCATGTCCTCATCACTGGCGCTGCCGGCTTCATCGGCCAATTGCTTGCGAAAGAGCTGTTGAATGACCCAGCC TATCGCGTCACACTGACCGATATACATCAACCTCCGATCCCCGCGGGCGTTCGCTATCCTCAATCCGCAACGGCA ATCAAGGTCGATCTCTTGACAGGCGCCAAGGACATTGTCGATTCTTCCTTGGACGCCGTCTATGCCTTTCACGGC ATCATGTCGTCCGGCTCCGAGGACAACTTCGATCTAGGCATGAGCGTCAACGTCGATGCAACCCGCAACCTACTC GAGGCGCTGCGACACACCTGTCCCGGAGTCCGATTCATCTATTCGTCCAGTCAGGCCGTATATGGCCGACCGTTG CCAGAGATTGTGACTGACAGCGTAATTCCGACCCCAGAGTCCTCTTACGGTGCCGAGAAGATCGTTTGCGAAACA TTGGTGAACGAATACACTCGCCGTAAATTCATCACCGGGTTTATCCTGCGGTTTCCTACCATCTCCGTCCGACCG GGTGCACCCACTGCTGCAGCTTCGTCCTTCCTTTCCGGCATGATCCGGGAACCTCTTGACGGCCAGCAATGTGTG ATCCCCATTAAAGACCGACAGTTCAAGTCGTGGCTATGCTCGCCCAAAACCCTTGTGGAAAATCTCCTTCTCACT CTCCGTCTTCCTGCGGACTCCGTTCCCCCGCACATTCGCCAGATTAATGTGCCTGGAATCTGCGTCACAGTCCAG GGCATGATGGATGCATTGGAAGCCGTGGGCGGTGCAGATAAACTCGCCCTTCTAACAGAGAAGGAGGACCCTGCG CTGGTTCCAATCTTGAAGTCATGGCCGACGCAATTTGATAACACTCAAGCCATCTCCTTGGGGTTCAAACGCGAC GAGTCTTTCGAGCAAACCGTGCGGGATTATAAACAGTCATTGACACAATATAGATCTTGA |