© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Ophun1|6239 |
| Gene name | |
| Location | Contig_67:7401..10015 |
| Strand | - |
| Gene length (bp) | 2614 |
| Transcript length (bp) | 1848 |
| Coding sequence length (bp) | 1848 |
| Protein length (aa) | 616 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF01073 | 3Beta_HSD | 3-beta hydroxysteroid dehydrogenase/isomerase family | 6.6E-25 | 232 | 508 |
| PF01370 | Epimerase | NAD dependent epimerase/dehydratase family | 1.5E-11 | 232 | 443 |
| PF07993 | NAD_binding_4 | Male sterility protein | 4.0E-07 | 308 | 429 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q9D665|D42E1_MOUSE | Short-chain dehydrogenase/reductase family 42E member 1 OS=Mus musculus GN=Sdr42e1 PE=1 SV=1 | 224 | 614 | 2.0E-15 |
| sp|A8DZE7|D42E1_DANRE | Short-chain dehydrogenase/reductase family 42E member 1 OS=Danio rerio GN=sdr42e1 PE=2 SV=1 | 227 | 614 | 3.0E-15 |
| sp|Q4R7R1|D42E1_MACFA | Short-chain dehydrogenase/reductase family 42E member 1 OS=Macaca fascicularis GN=SDR42E1 PE=2 SV=1 | 226 | 542 | 3.0E-13 |
| sp|Q8WUS8|D42E1_HUMAN | Short-chain dehydrogenase/reductase family 42E member 1 OS=Homo sapiens GN=SDR42E1 PE=2 SV=2 | 226 | 542 | 2.0E-12 |
| sp|Q32L94|D42E1_BOVIN | Short-chain dehydrogenase/reductase family 42E member 1 OS=Bos taurus GN=SDR42E1 PE=2 SV=2 | 232 | 509 | 9.0E-12 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q9D665|D42E1_MOUSE | Short-chain dehydrogenase/reductase family 42E member 1 OS=Mus musculus GN=Sdr42e1 PE=1 SV=1 | 224 | 614 | 2.0E-15 |
| sp|A8DZE7|D42E1_DANRE | Short-chain dehydrogenase/reductase family 42E member 1 OS=Danio rerio GN=sdr42e1 PE=2 SV=1 | 227 | 614 | 3.0E-15 |
| sp|Q4R7R1|D42E1_MACFA | Short-chain dehydrogenase/reductase family 42E member 1 OS=Macaca fascicularis GN=SDR42E1 PE=2 SV=1 | 226 | 542 | 3.0E-13 |
| sp|Q8WUS8|D42E1_HUMAN | Short-chain dehydrogenase/reductase family 42E member 1 OS=Homo sapiens GN=SDR42E1 PE=2 SV=2 | 226 | 542 | 2.0E-12 |
| sp|Q32L94|D42E1_BOVIN | Short-chain dehydrogenase/reductase family 42E member 1 OS=Bos taurus GN=SDR42E1 PE=2 SV=2 | 232 | 509 | 9.0E-12 |
| sp|Q9EQC1|3BHS7_MOUSE | 3 beta-hydroxysteroid dehydrogenase type 7 OS=Mus musculus GN=Hsd3b7 PE=1 SV=1 | 231 | 612 | 8.0E-11 |
| sp|Q9R1J0|NSDHL_MOUSE | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus GN=Nsdhl PE=1 SV=1 | 226 | 497 | 3.0E-10 |
| sp|Q3ZBE9|NSDHL_BOVIN | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Bos taurus GN=NSDHL PE=2 SV=1 | 227 | 497 | 7.0E-10 |
| sp|Q15738|NSDHL_HUMAN | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Homo sapiens GN=NSDHL PE=1 SV=2 | 229 | 497 | 2.0E-09 |
| sp|P26439|3BHS2_HUMAN | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 OS=Homo sapiens GN=HSD3B2 PE=1 SV=2 | 232 | 541 | 6.0E-09 |
| sp|Q0IH73|D42E1_XENLA | Short-chain dehydrogenase/reductase family 42E member 1 OS=Xenopus laevis GN=sdr42e1 PE=2 SV=1 | 232 | 499 | 7.0E-09 |
| sp|P9WQP7|3BHS_MYCTU | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=Rv1106c PE=1 SV=1 | 230 | 509 | 1.0E-08 |
| sp|Q5PPL3|NSDHL_RAT | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus GN=Nsdhl PE=2 SV=1 | 226 | 497 | 1.0E-08 |
| sp|P9WQP6|3BHS_MYCTO | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=MT1137 PE=3 SV=1 | 230 | 509 | 1.0E-08 |
| sp|O43050|ERG26_SCHPO | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=erg26 PE=3 SV=1 | 232 | 549 | 5.0E-08 |
| sp|Q67ZE1|HSDD2_ARATH | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana GN=3BETAHSD/D2 PE=2 SV=2 | 228 | 612 | 9.0E-08 |
| sp|O57245|3BHS_VACCA | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Vaccinia virus (strain Ankara) GN=MVA157L PE=3 SV=1 | 231 | 433 | 1.0E-07 |
| sp|P26670|3BHS_VACCW | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Vaccinia virus (strain Western Reserve) GN=VACWR170 PE=3 SV=1 | 231 | 433 | 1.0E-07 |
| sp|P21097|3BHS_VACCC | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Vaccinia virus (strain Copenhagen) GN=A44L PE=3 SV=1 | 231 | 433 | 1.0E-07 |
| sp|A6NKP2|D42E2_HUMAN | Putative short-chain dehydrogenase/reductase family 42E member 2 OS=Homo sapiens GN=SDR42E2 PE=3 SV=3 | 226 | 530 | 1.0E-07 |
| sp|O35048|3BHS7_RAT | 3 beta-hydroxysteroid dehydrogenase type 7 OS=Rattus norvegicus GN=Hsd3b7 PE=2 SV=1 | 231 | 612 | 2.0E-07 |
| sp|Q9H2F3|3BHS7_HUMAN | 3 beta-hydroxysteroid dehydrogenase type 7 OS=Homo sapiens GN=HSD3B7 PE=1 SV=2 | 221 | 612 | 3.0E-07 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0003854 | 3-beta-hydroxy-delta5-steroid dehydrogenase activity | Yes |
| GO:0006694 | steroid biosynthetic process | Yes |
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | Yes |
| GO:0016229 | steroid dehydrogenase activity | No |
| GO:0008610 | lipid biosynthetic process | No |
| GO:0044238 | primary metabolic process | No |
| GO:0006629 | lipid metabolic process | No |
| GO:0003824 | catalytic activity | No |
| GO:0009058 | biosynthetic process | No |
| GO:0008152 | metabolic process | No |
| GO:1901362 | organic cyclic compound biosynthetic process | No |
| GO:1901576 | organic substance biosynthetic process | No |
| GO:0003674 | molecular_function | No |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors | No |
| GO:0008202 | steroid metabolic process | No |
| GO:0008150 | biological_process | No |
| GO:0033764 | steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | No |
| GO:0016491 | oxidoreductase activity | No |
| GO:1901360 | organic cyclic compound metabolic process | No |
| GO:0071704 | organic substance metabolic process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 41 | 0.5 |
| Domain # | Start | End | Length |
|---|---|---|---|
| 1 | 20 | 42 | 22 |
| 2 | 157 | 179 | 22 |
| 3 | 231 | 253 | 22 |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Ophun1|6239 MRFKTRDMTKASMTTSFKFWKASSCMKLLVMAVFALTAMGAVPDVRLPRPSASRRLSNPAVYLNVTTPSTTVLIN ATLQEKPTEMTRPLSSSPSLPRPGLMSGPPFRNSTRDSHGMRPIRPHGYQACGNSTFLSAVPYCCNFGMGAADCK PASSTSTTGMGVITIAALLVAACLFLYALHINRVMKQTPDEALAVVDPPLTPHQARIIYRRVKEHGIDWNANLPP AKERRYLIVGGSGLVGGQIAVSLLATGVSRQAIRVVDLVPPVHDELSADKVAWVKADITSQASVDAAFAEPWPSA VSDLPLTVFHTAAVIRPFERDPIFYPRCARVNVLGSAHVLAAARKAGAGILIFTSSCNAAGSAVNWLFPPWNRRP REMAQRIGEDDYWKPLRPARCFPTTYAVSKAEAERMLCGADGADMRTAAIRPGNGVYGNRHDHIVSYMLALRVVP TFAAPWVQNWANVHNVAQAHLLLEEALLGEHADRVAGRPFMISDDGPPLRFQDVYTMLETTSSTDFRVQHPPPVL MLMAAYGIEAYCRLLARAGPLRRLLREPGDLVQMLQPGTISTTVTSLTDSSAARRRPDEGGLGYRPACTTLEGLS AQLLEWNEASRGKGL* |
| Coding | >Ophun1|6239 ATGCGCTTTAAGACGAGAGACATGACCAAGGCTTCGATGACGACATCATTCAAGTTCTGGAAGGCGAGCAGCTGC ATGAAGCTCCTCGTCATGGCCGTCTTCGCCCTGACTGCGATGGGCGCCGTTCCAGACGTACGTCTGCCCAGGCCT TCAGCCTCCAGACGATTGTCGAATCCAGCCGTGTATCTCAACGTAACGACGCCCAGCACAACGGTCCTGATCAAC GCGACATTGCAGGAAAAACCGACAGAAATGACCAGGCCGCTTTCTTCCTCGCCGTCGCTGCCACGTCCCGGACTG ATGAGCGGACCGCCCTTCAGGAACTCGACTCGTGACAGCCACGGGATGCGGCCCATCCGCCCGCACGGCTACCAG GCCTGCGGCAACTCGACCTTTTTGAGTGCCGTTCCGTACTGCTGTAACTTCGGTATGGGAGCGGCGGATTGCAAA CCCGCTTCGTCAACCTCGACGACCGGGATGGGCGTCATCACCATCGCCGCGCTGCTCGTGGCCGCGTGCCTCTTC CTCTATGCCCTGCACATCAACCGCGTCATGAAGCAGACGCCGGACGAGGCTCTCGCCGTTGTTGATCCGCCTCTG ACGCCTCATCAGGCCCGGATCATCTACCGGCGCGTCAAGGAGCATGGCATCGACTGGAACGCGAACCTGCCGCCA GCCAAAGAAAGACGCTACCTCATTGTCGGTGGCTCCGGTCTCGTGGGCGGGCAGATCGCCGTCTCTCTGCTGGCA ACGGGCGTGTCTCGTCAAGCCATACGGGTCGTGGACCTGGTTCCGCCCGTTCACGACGAGCTGAGCGCCGACAAG GTGGCCTGGGTCAAGGCCGATATCACGTCACAGGCGTCGGTGGATGCCGCCTTCGCCGAGCCGTGGCCATCAGCT GTCTCCGATCTACCGCTGACCGTCTTCCACACGGCCGCCGTCATCCGTCCCTTTGAGCGAGATCCCATCTTCTAC CCTCGTTGCGCCCGCGTCAACGTCCTCGGCTCAGCACACGTTCTGGCAGCCGCACGCAAGGCCGGCGCCGGCATC CTCATCTTCACGTCGAGCTGTAACGCGGCGGGAAGCGCCGTGAACTGGCTCTTCCCGCCTTGGAACCGACGTCCA CGCGAGATGGCGCAGCGCATCGGCGAGGACGATTACTGGAAGCCGTTGCGTCCGGCTCGGTGTTTCCCCACCACG TACGCCGTGTCCAAGGCCGAGGCGGAGCGAATGCTGTGTGGAGCCGACGGAGCCGATATGCGGACGGCGGCGATC CGGCCGGGCAACGGCGTGTACGGCAACCGACACGACCACATAGTCAGCTACATGCTGGCGCTGCGGGTAGTTCCG ACGTTTGCCGCTCCGTGGGTGCAGAACTGGGCCAACGTGCACAACGTCGCGCAGGCACATTTACTTCTCGAGGAG GCGCTGCTGGGCGAGCACGCAGATCGGGTAGCGGGACGGCCGTTCATGATATCAGACGACGGTCCGCCGCTCCGG TTCCAGGACGTTTACACGATGCTCGAGACGACGAGCTCGACCGACTTCCGGGTGCAGCATCCGCCGCCGGTGCTG ATGCTGATGGCGGCGTACGGCATCGAGGCCTACTGCCGGCTGCTGGCGAGGGCGGGTCCGCTGCGGCGACTGCTA CGGGAGCCGGGTGACTTGGTGCAGATGCTGCAGCCGGGAACGATTTCGACGACGGTGACGAGCCTGACGGACAGT TCGGCGGCGAGGCGGAGGCCGGACGAGGGCGGGCTGGGCTACCGGCCGGCGTGTACGACGCTGGAGGGACTGAGC GCGCAGCTGCTGGAGTGGAACGAGGCCAGCAGGGGCAAGGGGTTGTAG |
| Transcript | >Ophun1|6239 ATGCGCTTTAAGACGAGAGACATGACCAAGGCTTCGATGACGACATCATTCAAGTTCTGGAAGGCGAGCAGCTGC ATGAAGCTCCTCGTCATGGCCGTCTTCGCCCTGACTGCGATGGGCGCCGTTCCAGACGTACGTCTGCCCAGGCCT TCAGCCTCCAGACGATTGTCGAATCCAGCCGTGTATCTCAACGTAACGACGCCCAGCACAACGGTCCTGATCAAC GCGACATTGCAGGAAAAACCGACAGAAATGACCAGGCCGCTTTCTTCCTCGCCGTCGCTGCCACGTCCCGGACTG ATGAGCGGACCGCCCTTCAGGAACTCGACTCGTGACAGCCACGGGATGCGGCCCATCCGCCCGCACGGCTACCAG GCCTGCGGCAACTCGACCTTTTTGAGTGCCGTTCCGTACTGCTGTAACTTCGGTATGGGAGCGGCGGATTGCAAA CCCGCTTCGTCAACCTCGACGACCGGGATGGGCGTCATCACCATCGCCGCGCTGCTCGTGGCCGCGTGCCTCTTC CTCTATGCCCTGCACATCAACCGCGTCATGAAGCAGACGCCGGACGAGGCTCTCGCCGTTGTTGATCCGCCTCTG ACGCCTCATCAGGCCCGGATCATCTACCGGCGCGTCAAGGAGCATGGCATCGACTGGAACGCGAACCTGCCGCCA GCCAAAGAAAGACGCTACCTCATTGTCGGTGGCTCCGGTCTCGTGGGCGGGCAGATCGCCGTCTCTCTGCTGGCA ACGGGCGTGTCTCGTCAAGCCATACGGGTCGTGGACCTGGTTCCGCCCGTTCACGACGAGCTGAGCGCCGACAAG GTGGCCTGGGTCAAGGCCGATATCACGTCACAGGCGTCGGTGGATGCCGCCTTCGCCGAGCCGTGGCCATCAGCT GTCTCCGATCTACCGCTGACCGTCTTCCACACGGCCGCCGTCATCCGTCCCTTTGAGCGAGATCCCATCTTCTAC CCTCGTTGCGCCCGCGTCAACGTCCTCGGCTCAGCACACGTTCTGGCAGCCGCACGCAAGGCCGGCGCCGGCATC CTCATCTTCACGTCGAGCTGTAACGCGGCGGGAAGCGCCGTGAACTGGCTCTTCCCGCCTTGGAACCGACGTCCA CGCGAGATGGCGCAGCGCATCGGCGAGGACGATTACTGGAAGCCGTTGCGTCCGGCTCGGTGTTTCCCCACCACG TACGCCGTGTCCAAGGCCGAGGCGGAGCGAATGCTGTGTGGAGCCGACGGAGCCGATATGCGGACGGCGGCGATC CGGCCGGGCAACGGCGTGTACGGCAACCGACACGACCACATAGTCAGCTACATGCTGGCGCTGCGGGTAGTTCCG ACGTTTGCCGCTCCGTGGGTGCAGAACTGGGCCAACGTGCACAACGTCGCGCAGGCACATTTACTTCTCGAGGAG GCGCTGCTGGGCGAGCACGCAGATCGGGTAGCGGGACGGCCGTTCATGATATCAGACGACGGTCCGCCGCTCCGG TTCCAGGACGTTTACACGATGCTCGAGACGACGAGCTCGACCGACTTCCGGGTGCAGCATCCGCCGCCGGTGCTG ATGCTGATGGCGGCGTACGGCATCGAGGCCTACTGCCGGCTGCTGGCGAGGGCGGGTCCGCTGCGGCGACTGCTA CGGGAGCCGGGTGACTTGGTGCAGATGCTGCAGCCGGGAACGATTTCGACGACGGTGACGAGCCTGACGGACAGT TCGGCGGCGAGGCGGAGGCCGGACGAGGGCGGGCTGGGCTACCGGCCGGCGTGTACGACGCTGGAGGGACTGAGC GCGCAGCTGCTGGAGTGGAACGAGGCCAGCAGGGGCAAGGGGTTGTAG |
| Gene | >Ophun1|6239 ATGCGCTTTAAGACGAGAGACATGACCAAGGCTTCGATGACGACATCATTCAAGTTCTGGAAGGCGAGCAGCTGG TAAACACGGCCTACTCTCCTCGTGGACGAACGACTGAGACTTGGATGACGGCATTATTCAGAGTCTAGACTGCGA GCAGCTGACGATAAATACGGCCGACTCCCCTCGTAGACGAACGATTCCTATCCCTCCCCGCTCCTCAAACATCCA TCGACAGCATGAAGCTCCTCGTCATGGCCGTCTTCGCCCTGACTGCGATGGGCGCCGTTCCAGACGTACGTCTGC CCAGGCCTTCAGCCTCCAGACGATTGTCGAATCCAGCCGTGTATCTCAACGTAACGACGCCCAGCACAACGGTCC TGATCAACGCGACATTGCAGGAAAAACCGACAGAAATGACCAGGCCGCTTTCTTCCTCGCCGTCGCTGCCACGTC CCGGACTGATGAGCGGACCGCCCTTCAGGAACTCGACTCGTGACAGCCACGGGATGCGGCCCATCCGCCCGCACG GCTACCAGGCCTGCGGCAACTCGACCTTTTTGAGTGCCGTTCCGTACTGCTGTAACTTCGGTATGGGAGCGGCGG ATTGCAAACCCGGTACGTCGAGCCCCGTTCTCGTAGTCTGTCTCTCCTGTGCTGATGGGGGTCTTCTTCGGGCTT GCCAGCGACGGGAGAGCCTACCAGCTCCGGTAGCTTCATCAGCATGTGTCAGCCTCAGGGCCAGCCCAAGTGCTG TTTCAAAAGCTTCAACGTAGGTGGAATGGATCCTGGTGACGGCTGATTGTGTTTGCTGACCATGGCTGGAAGGGG GTGCTCATGTGTCTCGGTTATGTCGACCGGTGAGAGGGAGCAAGAGAGCAGAGGGGACGATGGTTGTAATGCAGG AGAGAGAGAGGGTCCGAGAAGAGTAAATAACTCTAGTGGAGGAGGGCAGAGTTGGGTATGACTATGAGGCAGGGT TGTAGTCTGTGGTAGACTGGCACGTGATGAGGCCGTTGAGGCGGCTCTGTGCCGAGCACGAATATTCGTAGCTTA ATCGATTCACGGAAAAAATCTCACTTTCATCTCACTTCACTCATCATGTCGTGACCATCGCCGCTGTTCATCTCT CCTCTTGACCCTGCAGCTTCGTCAACCTCGACGACCGGGATGGGCGTCATCACCATCGCCGCGCTGCTCGTGGCC GCGTGCCTCTTCCTCTATGCCCTGCACATCAACCGCGTCATGAAGCAGACGCCGGACGAGGCTCTCGCCGTTGTT GATCCGCCTCTGACGCCTCATCAGGCCCGGATCATCTACCGGCGCGTCAAGGAGCATGGCATCGACTGGAACGCG AACCTGCCGCCAGCCAAAGAAAGACGCTACCTCATTGTCGGTGGCTCCGGTGAGCTTATCCAAGTCCTCGTTGTC TTTCCTCGTTGTCTTGGCTATGTTTCGGAGCTGACTGAGTCGCCATTCAACAGGTCTCGTGGGCGGGCAGATCGC CGTCTCTCTGCTGGCAACGGGCGTGTCTCGTCAAGCCATACGGGTCGTGGACCTGGTTCCGCCCGTTCACGACGA GCTGAGCGCCGACAAGGTGGCCTGGGTCAAGGCCGATATCACGTCACAGGCGTCGGTGGATGCCGCCTTCGCCGA GCCGTGGCCATCAGCTGTCTCCGATCTACCGCTGACCGTCTTCCACACGGCCGCCGTCATCCGTCCCTTTGAGCG AGATCCCATCTTCTACCCTCGTTGCGCCCGCGTCAACGTCCTCGGCTCAGCACACGTTCTGGCAGCCGCACGCAA GGCCGGCGCCGGCATCCTCATCTTCACGTCGAGCTGTAACGCGGCGGGAAGCGCCGTGAACTGGCTCTTCCCGCC TTGGAACCGACGTCCACGCGAGATGGCGCAGCGCATCGGCGAGGACGATTACTGGAAGCCGTTGCGTCCGGCTCG GTGTTTCCCCACCACGTACGCCGTGTCCAAGGCCGAGGCGGAGCGAATGCTGTGTGGAGCCGACGGAGCCGATAT GCGGACGGCGGCGATCCGGCCGGGCAACGGCGTGTACGGCAACCGACACGACCACATAGTCAGCTACATGCTGGC GCTGCGGGTAGTTCCGACGTTTGCCGCTCCGTGGGTGCAGAACTGGGCCAACGTGCACAACGTCGCGCAGGCACA TTTACTTCTCGAGGAGGCGCTGCTGGGCGAGCACGCAGATCGGGTAGCGGGACGGCCGTTCATGATATCAGACGA CGGTCCGCCGCTCCGGTTCCAGGACGTTTACACGATGCTCGAGACGACGAGCTCGACCGACTTCCGGGTGCAGCA TCCGCCGCCGGTGCTGATGCTGATGGCGGCGTACGGCATCGAGGCCTACTGCCGGCTGCTGGCGAGGGCGGGTCC GCTGCGGCGACTGCTACGGGAGCCGGGTGACTTGGTGCAGATGCTGCAGCCGGGAACGATTTCGACGACGGTGAC GAGCCTGACGGACAGTTCGGCGGCGAGGCGGAGGCCGGACGAGGGCGGGCTGGGCTACCGGCCGGCGTGTACGAC GCTGGAGGGACTGAGCGCGCAGCTGCTGGAGTGGAACGAGGCCAGCAGGGGCAAGGGGTTGTAG |