Fungal Genomics

at Utrecht University

General Properties

Protein ID	Ophun1\|6086
Gene name
Location	Contig_64:27350..30304
Strand	+
Gene length (bp)	2954
Transcript length (bp)	2625
Coding sequence length (bp)	2625
Protein length (aa)	875

PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF04734	Ceramidase_alk	Neutral/alkaline non-lysosomal ceramidase, N-terminal	2.1E-187	183	705
PF17048	Ceramidse_alk_C	Neutral/alkaline non-lysosomal ceramidase, C-terminal	7.4E-47	711	870

Swissprot hits

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Swissprot ID	Swissprot Description	Start	End	E-value
sp\|Q304B9\|NCASE_ARATH	Neutral ceramidase OS=Arabidopsis thaliana GN=At2g38010 PE=3 SV=1	183	854	2.0E-124
sp\|Q5W7F1\|ASAH2_DANRE	Neutral ceramidase OS=Danio rerio GN=asah2 PE=1 SV=1	171	871	2.0E-113
sp\|Q54BK2\|NCSEA_DICDI	Neutral ceramidase A OS=Dictyostelium discoideum GN=dcd2A PE=1 SV=1	178	859	3.0E-109
sp\|Q55G11\|NCSEB_DICDI	Neutral ceramidase B OS=Dictyostelium discoideum GN=dcd2B PE=3 SV=1	171	869	4.0E-108
sp\|Q9VA70\|NCASE_DROME	Neutral ceramidase OS=Drosophila melanogaster GN=CDase PE=1 SV=1	180	871	1.0E-105

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Swissprot ID	Swissprot Description	Start	End	E-value
sp\|Q304B9\|NCASE_ARATH	Neutral ceramidase OS=Arabidopsis thaliana GN=At2g38010 PE=3 SV=1	183	854	2.0E-124
sp\|Q5W7F1\|ASAH2_DANRE	Neutral ceramidase OS=Danio rerio GN=asah2 PE=1 SV=1	171	871	2.0E-113
sp\|Q54BK2\|NCSEA_DICDI	Neutral ceramidase A OS=Dictyostelium discoideum GN=dcd2A PE=1 SV=1	178	859	3.0E-109
sp\|Q55G11\|NCSEB_DICDI	Neutral ceramidase B OS=Dictyostelium discoideum GN=dcd2B PE=3 SV=1	171	869	4.0E-108
sp\|Q9VA70\|NCASE_DROME	Neutral ceramidase OS=Drosophila melanogaster GN=CDase PE=1 SV=1	180	871	1.0E-105
sp\|Q29C43\|NCASE_DROPS	Neutral ceramidase OS=Drosophila pseudoobscura pseudoobscura GN=CDase PE=3 SV=1	180	871	6.0E-103
sp\|Q9NR71\|ASAH2_HUMAN	Neutral ceramidase OS=Homo sapiens GN=ASAH2 PE=1 SV=2	183	871	9.0E-102
sp\|Q9JHE3\|ASAH2_MOUSE	Neutral ceramidase OS=Mus musculus GN=Asah2 PE=1 SV=1	162	872	5.0E-100
sp\|Q91XT9\|ASAH2_RAT	Neutral ceramidase OS=Rattus norvegicus GN=Asah2 PE=1 SV=1	177	872	1.0E-92
sp\|Q9I596\|NCASE_PSEAE	Neutral ceramidase OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN=PA0845 PE=1 SV=1	183	871	2.0E-86
sp\|O06769\|NCASE_MYCTU	Neutral ceramidase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=Rv0669c PE=1 SV=1	185	872	1.0E-76
sp\|Q0JL46\|NCASE_ORYSJ	Neutral ceramidase OS=Oryza sativa subsp. japonica GN=Os01g0624000 PE=1 SV=1	406	871	4.0E-72
sp\|Q0JL46\|NCASE_ORYSJ	Neutral ceramidase OS=Oryza sativa subsp. japonica GN=Os01g0624000 PE=1 SV=1	183	417	3.0E-40
sp\|P0C7U1\|ASA2B_HUMAN	Putative inactive neutral ceramidase B OS=Homo sapiens GN=ASAH2B PE=2 SV=1	753	873	2.0E-08

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GO

(None)

SignalP

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SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
No	1 - 61	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

No expression data available for this genome

Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>Ophun1\|6086 MSVKSRSAPSPPSSSDFQESPQINAEPVVAESIVAEPPVAQPPVAKPIVANPSVAKPSVAKPPVVADGFTGVSPS RQLPGRIFEKPQPRPQPSVLALPVAKPFVAEPSVVSGGLAGASPSRQPPERTFKKQQPRPQPSVLEPPVFGPSVV PDGFTGTGSGRQPPERTFKNQQPRPQPSQSNRFLIGVGKADITGPVAELVMAGYAHDDQIASGLRQRIYSRAFII ADVNRPQERLLYLVLDNINSDTSIRTGLLEALANGGDELSYYNQHNLALSSVHSHSGPSTGWNYFLAQVPQSGFD RQGQQAVIDGALLSIKRAQSGLQEGYLDLAEIDIPDASVSRSLWAYLANPVEERAKYGREIDTTMTGLRFRQASD GKTKAILTWFPVHGTSLYGNNTHISGDNKGLASWMLEQKLRQSPAFTKDFIGAFGQANEADVSPNTDGAWCEDGS GQPCNYTDSSCPDGLVTKCHGRGPMFRALDLGVSSCHEIARRQAVKAEEMLVSPLTRWTPIDAQLGVKGYHFYHD MAFWRFKTLEGREAMTCPAALGFSFAAGTTDGPGLFDFVQGDANPVPKNPLWTFAFGFVKAASRRQKICQVPKPI LFNGGQLSSPYDWSPNIVDVAMFRIGQLFIILSPSELTTMSGRRWKEAIRKEADKFIQRVRPIVVAASPVNSYSH YLATKEEYDIQRYEGASTLFGRWQLDAFMNLSVSSMPYLVPGNQNFPEQGTLPPDNRKKSFNSNNPVSPDLRSRT PGYGAVLRQPNPRYVIGQNVQATFQGANPRNNLRLEDTFAAVEQLGKDGKWTRVRDDDDWFLVFTWRRTGNFFKR TEEVDITWETKGNAKPGIYRLKYYGDAKTRYQGLQPIEGTSNSFSLTAR*
Coding	>Ophun1\|6086 ATGTCAGTAAAAAGCCGCTCCGCTCCCAGTCCGCCAAGTTCTTCCGATTTCCAAGAAAGTCCGCAGATCAACGCT GAGCCGGTTGTCGCTGAGTCGATTGTCGCTGAGCCACCTGTCGCTCAGCCGCCTGTCGCCAAGCCGATTGTCGCC AATCCGTCAGTCGCCAAGCCGTCAGTCGCCAAGCCGCCTGTCGTCGCGGATGGCTTCACCGGCGTCAGTCCCAGT CGACAGCTTCCGGGGCGCATCTTCGAAAAGCCGCAACCAAGACCTCAGCCCTCTGTCCTTGCGCTGCCTGTTGCT AAGCCCTTTGTCGCTGAGCCCTCTGTCGTCTCGGGCGGCCTCGCTGGCGCCAGCCCCAGTCGACAGCCTCCGGAG CGTACCTTCAAGAAGCAGCAGCCGAGGCCTCAACCCTCTGTCCTTGAGCCGCCTGTCTTCGGGCCCTCTGTCGTC CCGGATGGCTTCACAGGCACCGGCTCCGGTCGACAGCCTCCGGAGCGCACATTCAAGAACCAGCAACCAAGGCCT CAACCCTCTCAAAGCAACAGGTTTCTCATCGGCGTCGGCAAAGCCGACATCACTGGACCCGTCGCCGAGCTCGTC ATGGCTGGCTATGCGCACGACGACCAAATAGCATCGGGGCTCCGACAGCGTATATACAGTCGAGCTTTCATCATT GCCGATGTCAACAGGCCACAAGAGCGCCTACTGTATCTGGTTCTTGACAATATCAACAGTGACACGTCCATCCGT ACCGGTCTCCTCGAAGCACTTGCTAACGGTGGGGACGAACTGTCCTACTACAATCAACACAACCTTGCCCTCTCG TCTGTTCACAGCCACTCTGGTCCCTCAACCGGCTGGAATTACTTTTTGGCTCAGGTCCCACAGTCTGGATTCGAT CGCCAGGGTCAACAGGCTGTCATCGATGGCGCGCTTCTTTCCATCAAACGAGCCCAAAGCGGTCTGCAAGAGGGT TATCTCGATCTCGCTGAAATCGACATCCCCGATGCCAGCGTCAGTCGCTCTCTATGGGCCTATCTTGCCAACCCG GTCGAGGAGAGAGCCAAATATGGGCGAGAGATAGACACCACAATGACTGGGCTTCGTTTCCGGCAGGCATCGGAT GGCAAGACCAAAGCCATCTTGACCTGGTTTCCCGTTCATGGCACCTCTCTCTACGGTAATAACACGCATATATCA GGCGACAACAAGGGTCTGGCTTCTTGGATGCTCGAGCAGAAGCTACGTCAGTCTCCCGCCTTCACAAAGGACTTT ATCGGTGCTTTCGGCCAAGCAAATGAGGCCGACGTCTCCCCCAACACCGACGGGGCCTGGTGCGAGGACGGCTCC GGACAGCCGTGCAACTACACCGACTCTTCATGTCCCGACGGCCTGGTAACAAAATGCCACGGTAGAGGTCCCATG TTTCGGGCCTTGGACTTGGGCGTCAGCAGCTGCCACGAGATCGCCCGGCGACAGGCTGTCAAAGCCGAGGAAATG TTGGTTTCGCCCCTCACTCGTTGGACACCGATCGATGCGCAACTCGGTGTCAAAGGCTACCACTTCTATCACGAT ATGGCCTTTTGGAGATTCAAGACACTAGAAGGTCGCGAGGCCATGACGTGTCCCGCTGCCCTGGGATTCTCTTTT GCTGCCGGCACGACTGATGGCCCAGGGCTATTCGACTTTGTCCAGGGGGACGCTAATCCGGTGCCCAAGAATCCG CTGTGGACATTCGCCTTTGGCTTCGTCAAGGCTGCCAGCCGAAGACAGAAAATCTGCCAAGTGCCCAAGCCCATC TTATTCAACGGCGGCCAACTATCGTCTCCTTACGATTGGTCGCCCAACATCGTCGACGTTGCCATGTTTCGGATC GGCCAGCTATTCATTATTCTCAGCCCGAGCGAACTGACCACCATGAGCGGTCGACGATGGAAAGAGGCCATCAGA AAGGAAGCCGACAAGTTTATCCAACGTGTCCGCCCTATCGTCGTCGCTGCCAGCCCGGTCAACTCTTACTCCCAT TATCTCGCGACCAAGGAGGAGTACGACATTCAGCGGTACGAAGGGGCCTCGACTTTGTTTGGCCGCTGGCAGCTT GACGCCTTCATGAACCTGAGTGTCAGTAGCATGCCGTATCTGGTGCCGGGCAACCAAAATTTTCCAGAGCAAGGC ACGCTGCCTCCGGATAACCGGAAAAAATCGTTCAACAGCAACAATCCCGTCTCCCCAGACCTCAGATCGCGGACA CCGGGGTATGGAGCGGTCCTGAGACAGCCTAATCCAAGATACGTCATCGGACAAAACGTGCAAGCCACTTTCCAA GGGGCGAATCCTCGTAACAACCTACGGTTAGAAGATACATTCGCCGCGGTCGAGCAACTCGGCAAAGACGGGAAG TGGACGCGCGTACGCGATGATGATGACTGGTTCCTAGTATTCACATGGCGGAGGACAGGTAACTTCTTCAAGCGG ACCGAGGAGGTCGACATAACGTGGGAAACAAAGGGAAACGCGAAGCCGGGAATCTATCGGCTCAAGTATTACGGC GATGCGAAGACGAGATACCAGGGACTTCAGCCAATCGAGGGCACGAGCAACAGTTTTTCGCTGACTGCCAGGTAA
Transcript	>Ophun1\|6086 ATGTCAGTAAAAAGCCGCTCCGCTCCCAGTCCGCCAAGTTCTTCCGATTTCCAAGAAAGTCCGCAGATCAACGCT GAGCCGGTTGTCGCTGAGTCGATTGTCGCTGAGCCACCTGTCGCTCAGCCGCCTGTCGCCAAGCCGATTGTCGCC AATCCGTCAGTCGCCAAGCCGTCAGTCGCCAAGCCGCCTGTCGTCGCGGATGGCTTCACCGGCGTCAGTCCCAGT CGACAGCTTCCGGGGCGCATCTTCGAAAAGCCGCAACCAAGACCTCAGCCCTCTGTCCTTGCGCTGCCTGTTGCT AAGCCCTTTGTCGCTGAGCCCTCTGTCGTCTCGGGCGGCCTCGCTGGCGCCAGCCCCAGTCGACAGCCTCCGGAG CGTACCTTCAAGAAGCAGCAGCCGAGGCCTCAACCCTCTGTCCTTGAGCCGCCTGTCTTCGGGCCCTCTGTCGTC CCGGATGGCTTCACAGGCACCGGCTCCGGTCGACAGCCTCCGGAGCGCACATTCAAGAACCAGCAACCAAGGCCT CAACCCTCTCAAAGCAACAGGTTTCTCATCGGCGTCGGCAAAGCCGACATCACTGGACCCGTCGCCGAGCTCGTC ATGGCTGGCTATGCGCACGACGACCAAATAGCATCGGGGCTCCGACAGCGTATATACAGTCGAGCTTTCATCATT GCCGATGTCAACAGGCCACAAGAGCGCCTACTGTATCTGGTTCTTGACAATATCAACAGTGACACGTCCATCCGT ACCGGTCTCCTCGAAGCACTTGCTAACGGTGGGGACGAACTGTCCTACTACAATCAACACAACCTTGCCCTCTCG TCTGTTCACAGCCACTCTGGTCCCTCAACCGGCTGGAATTACTTTTTGGCTCAGGTCCCACAGTCTGGATTCGAT CGCCAGGGTCAACAGGCTGTCATCGATGGCGCGCTTCTTTCCATCAAACGAGCCCAAAGCGGTCTGCAAGAGGGT TATCTCGATCTCGCTGAAATCGACATCCCCGATGCCAGCGTCAGTCGCTCTCTATGGGCCTATCTTGCCAACCCG GTCGAGGAGAGAGCCAAATATGGGCGAGAGATAGACACCACAATGACTGGGCTTCGTTTCCGGCAGGCATCGGAT GGCAAGACCAAAGCCATCTTGACCTGGTTTCCCGTTCATGGCACCTCTCTCTACGGTAATAACACGCATATATCA GGCGACAACAAGGGTCTGGCTTCTTGGATGCTCGAGCAGAAGCTACGTCAGTCTCCCGCCTTCACAAAGGACTTT ATCGGTGCTTTCGGCCAAGCAAATGAGGCCGACGTCTCCCCCAACACCGACGGGGCCTGGTGCGAGGACGGCTCC GGACAGCCGTGCAACTACACCGACTCTTCATGTCCCGACGGCCTGGTAACAAAATGCCACGGTAGAGGTCCCATG TTTCGGGCCTTGGACTTGGGCGTCAGCAGCTGCCACGAGATCGCCCGGCGACAGGCTGTCAAAGCCGAGGAAATG TTGGTTTCGCCCCTCACTCGTTGGACACCGATCGATGCGCAACTCGGTGTCAAAGGCTACCACTTCTATCACGAT ATGGCCTTTTGGAGATTCAAGACACTAGAAGGTCGCGAGGCCATGACGTGTCCCGCTGCCCTGGGATTCTCTTTT GCTGCCGGCACGACTGATGGCCCAGGGCTATTCGACTTTGTCCAGGGGGACGCTAATCCGGTGCCCAAGAATCCG CTGTGGACATTCGCCTTTGGCTTCGTCAAGGCTGCCAGCCGAAGACAGAAAATCTGCCAAGTGCCCAAGCCCATC TTATTCAACGGCGGCCAACTATCGTCTCCTTACGATTGGTCGCCCAACATCGTCGACGTTGCCATGTTTCGGATC GGCCAGCTATTCATTATTCTCAGCCCGAGCGAACTGACCACCATGAGCGGTCGACGATGGAAAGAGGCCATCAGA AAGGAAGCCGACAAGTTTATCCAACGTGTCCGCCCTATCGTCGTCGCTGCCAGCCCGGTCAACTCTTACTCCCAT TATCTCGCGACCAAGGAGGAGTACGACATTCAGCGGTACGAAGGGGCCTCGACTTTGTTTGGCCGCTGGCAGCTT GACGCCTTCATGAACCTGAGTGTCAGTAGCATGCCGTATCTGGTGCCGGGCAACCAAAATTTTCCAGAGCAAGGC ACGCTGCCTCCGGATAACCGGAAAAAATCGTTCAACAGCAACAATCCCGTCTCCCCAGACCTCAGATCGCGGACA CCGGGGTATGGAGCGGTCCTGAGACAGCCTAATCCAAGATACGTCATCGGACAAAACGTGCAAGCCACTTTCCAA GGGGCGAATCCTCGTAACAACCTACGGTTAGAAGATACATTCGCCGCGGTCGAGCAACTCGGCAAAGACGGGAAG TGGACGCGCGTACGCGATGATGATGACTGGTTCCTAGTATTCACATGGCGGAGGACAGGTAACTTCTTCAAGCGG ACCGAGGAGGTCGACATAACGTGGGAAACAAAGGGAAACGCGAAGCCGGGAATCTATCGGCTCAAGTATTACGGC GATGCGAAGACGAGATACCAGGGACTTCAGCCAATCGAGGGCACGAGCAACAGTTTTTCGCTGACTGCCAGGTAA
Gene	>Ophun1\|6086 ATGTCAGTAAAAAGCCGCTCCGCTCCCAGTCCGCCAAGTTCTTGTGAGCGCAACTTGCGAAGGTCCAGCCAAGAT GGTTTCTTTGCGCCCTGCTCTGACGGCTCTGCGGCCTCGCTTGCTTGTCTGGCTGTTGCTCGTGCTCTGTCTTGG TGATGCATTCGCTCTCTCTGATCAGTCTCCTCGAAACGACCAAGGCGGGCTTGTTCCAGATACCTCGCCGCCCCA ACTTGAGCTGGCTTCTGTCCCAGCCGATTTCCAAGAAAGTCCGCAGATCAACGCTGAGCCGGTTGTCGCTGAGTC GATTGTCGCTGAGCCACCTGTCGCTCAGCCGCCTGTCGCCAAGCCGATTGTCGCCAATCCGTCAGTCGCCAAGCC GTCAGTCGCCAAGCCGCCTGTCGTCGCGGATGGCTTCACCGGCGTCAGTCCCAGTCGACAGCTTCCGGGGCGCAT CTTCGAAAAGCCGCAACCAAGACCTCAGCCCTCTGTCCTTGCGCTGCCTGTTGCTAAGCCCTTTGTCGCTGAGCC CTCTGTCGTCTCGGGCGGCCTCGCTGGCGCCAGCCCCAGTCGACAGCCTCCGGAGCGTACCTTCAAGAAGCAGCA GCCGAGGCCTCAACCCTCTGTCCTTGAGCCGCCTGTCTTCGGGCCCTCTGTCGTCCCGGATGGCTTCACAGGCAC CGGCTCCGGTCGACAGCCTCCGGAGCGCACATTCAAGAACCAGCAACCAAGGCCTCAACCCTCTCAAAGCAACAG GTTTCTCATCGGCGTCGGCAAAGCCGACATCACTGGACCCGTCGCCGAGCTCGTCATGGCTGGCTATGCGCACGA CGACCAAATAGCATCGGGGCTCCGACAGCGTATATACAGTCGAGCTTTCATCATTGCCGATGTCAACAGGCCACA AGAGCGCCTACTGTATCTGGTTCTTGACAATATCAACAGTGACACGTCCATCCGTACCGGTCTCCTCGAAGCACT TGCTAACGGTGGGGACGAACTGTCCTACTACAATCAACACAACCTTGCCCTCTCGTCTGTTCACAGCCACTCTGG TCCCTCAACCGGCTGGAATTACTTTTTGGCTCAGGTCCCACAGTCTGGATTCGATCGCCAGGGTCAACAGGCTGT CATCGATGGCGCGCTTCTTTCCATCAAACGAGCCCAAAGCGGTCTGCAAGAGGTGAGTCTTGCCCTTTACGAGGT GTTTTGCTCTTTGTCTCGACGTCCATTAACCCGCACAGGGTTATCTCGATCTCGCTGAAATCGACATCCCCGATG CCAGCGTCAGTCGCTCTCTATGGGCCTATCTTGCCAACCCGGTCGAGGAGAGAGCCAAATATGGGCGAGAGATAG ACACCACAATGACTGGGCTTCGTTTCCGGCAGGCATCGGATGGCAAGACCAAAGCCATCTTGACCTGGTTTCCCG TTCATGGCACCTCTCTCTACGGTAATAACACGCATATATCAGGCGACAACAAGGGTCTGGCTTCTTGGATGCTCG AGCAGAAGCTACGTCAGTCTCCCGCCTTCACAAAGGACTTTATCGGTGCTTTCGGCCAAGCAAATGAGGCCGACG TCTCCCCCAACACCGACGGGGCCTGGTGCGAGGACGGCTCCGGACAGCCGTGCAACTACACCGACTCTTCATGTC CCGACGGCCTGGTAACAAAATGCCACGGTAGAGGTCCCATGTTTCGGGCCTTGGACTTGGGCGTCAGCAGCTGCC ACGAGATCGCCCGGCGACAGGCTGTCAAAGCCGAGGAAATGTTGGTAAGTGAAACGCCCCTAGTCATCCATGCCG TTCGTCTATCTGACTGCTGTTGCGTTTTCCAGGTTTCGCCCCTCACTCGTTGGACACCGATCGATGCGCAACTCG GTGTCAAAGGCTACCACTTCTATCACGATATGGCCTTTTGGAGATTCAAGACACTAGAAGGTCGCGAGGCCATGA CGTGTCCCGCTGCCCTGGGATTCTCTTTTGCTGCCGGCACGACTGATGGCCCAGGGCTATTCGACTTTGTCCAGG GGGACGCTAATCCGGTGCCCAAGAATCCGCTGTGGACATTCGCCTTTGGCTTCGTCAAGGCTGCCAGCCGAAGAC AGAAAATCTGCCAAGTGCCCAAGCCCATCTTATTCAACGGCGGCCAACTATCGTCTCCTTACGATTGGTCGCCCA ACATCGTCGACGTTGCCATGTTTCGGATCGGCCAGCTATTCATTATTCTCAGCCCGAGCGAACTGACCACCATGA GCGGTCGACGATGGAAAGAGGCCATCAGAAAGGAAGCCGACAAGTTTATCCAACGTGTCCGCCCTATCGTCGTCG CTGCCAGCCCGGTCAACTCTTACTCCCATTATCTCGCGACCAAGGAGGAGTACGACATTCAGCGGTACGAAGGGG CCTCGACTTTGTTTGGCCGCTGGCAGCTTGACGCCTTCATGAACCTGAGTGTCAGTAGCATGCCGTATCTGGTGC CGGGCAACCAAAATTTTCCAGAGCAAGGCACGCTGCCTCCGGATAACCGGAAAAAATCGTTCAACAGCAACAATC CCGTCTCCCCAGACCTCAGATCGCGGACACCGGGGTATGGAGCGGTCCTGAGACAGCCTAATCCAAGATACGTCA TCGGACAAAACGTGCAAGCCACTTTCCAAGGGGCGAATCCTCGTAACAACCTACGGTTAGAAGATACATTCGCCG CGGTCGAGCAACTCGGCAAAGACGGGAAGTGGACGCGCGTACGCGATGATGATGACTGGTTCCTAGTATTCACAT GGCGGAGGACAGGTAACTTCTTCAAGCGGACCGAGGAGGTCGACATAACGTGGGAAACAAAGGGAAACGCGAAGC CGGGAATCTATCGGCTCAAGTATTACGGCGATGCGAAGACGAGATACCAGGGACTTCAGCCAATCGAGGGCACGA GCAACAGTTTTTCGCTGACTGCCAGGTAA

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