© 2023 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Ophio5|6621 |
| Gene name | |
| Location | scaffold_59:48339..49690 |
| Strand | + |
| Gene length (bp) | 1351 |
| Transcript length (bp) | 1149 |
| Coding sequence length (bp) | 1146 |
| Protein length (aa) | 382 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF00173 | Cyt-b5 | Cytochrome b5-like Heme/Steroid binding domain | 6.2E-18 | 10 | 82 |
| PF04116 | FA_hydroxylase | Fatty acid hydroxylase | 1.1E-11 | 226 | 368 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q03529|SCS7_YEAST | Ceramide very long chain fatty acid hydroxylase SCS7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCS7 PE=1 SV=1 | 3 | 372 | 1.0E-131 |
| sp|O13846|SCS7_SCHPO | Ceramide very long chain fatty acid hydroxylase-like protein C19G12.08 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC19G12.08 PE=3 SV=1 | 19 | 369 | 8.0E-101 |
| sp|Q9SUC5|FAH2_ARATH | Fatty acid 2-hydroxylase 2 OS=Arabidopsis thaliana GN=FAH2 PE=1 SV=1 | 142 | 380 | 5.0E-49 |
| sp|Q5MPP0|FA2H_MOUSE | Fatty acid 2-hydroxylase OS=Mus musculus GN=Fa2h PE=1 SV=1 | 8 | 368 | 2.0E-47 |
| sp|Q2LAM0|FA2H_RAT | Fatty acid 2-hydroxylase OS=Rattus norvegicus GN=Fa2h PE=1 SV=2 | 8 | 368 | 6.0E-47 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q03529|SCS7_YEAST | Ceramide very long chain fatty acid hydroxylase SCS7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCS7 PE=1 SV=1 | 3 | 372 | 1.0E-131 |
| sp|O13846|SCS7_SCHPO | Ceramide very long chain fatty acid hydroxylase-like protein C19G12.08 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC19G12.08 PE=3 SV=1 | 19 | 369 | 8.0E-101 |
| sp|Q9SUC5|FAH2_ARATH | Fatty acid 2-hydroxylase 2 OS=Arabidopsis thaliana GN=FAH2 PE=1 SV=1 | 142 | 380 | 5.0E-49 |
| sp|Q5MPP0|FA2H_MOUSE | Fatty acid 2-hydroxylase OS=Mus musculus GN=Fa2h PE=1 SV=1 | 8 | 368 | 2.0E-47 |
| sp|Q2LAM0|FA2H_RAT | Fatty acid 2-hydroxylase OS=Rattus norvegicus GN=Fa2h PE=1 SV=2 | 8 | 368 | 6.0E-47 |
| sp|O48916|FAH1_ARATH | Fatty acid 2-hydroxylase 1 OS=Arabidopsis thaliana GN=FAH1 PE=1 SV=1 | 142 | 368 | 3.0E-46 |
| sp|Q4R4P4|FA2H_MACFA | Fatty acid 2-hydroxylase OS=Macaca fascicularis GN=FA2H PE=2 SV=1 | 13 | 368 | 6.0E-46 |
| sp|Q7L5A8|FA2H_HUMAN | Fatty acid 2-hydroxylase OS=Homo sapiens GN=FA2H PE=1 SV=1 | 13 | 368 | 1.0E-44 |
| sp|Q9ZWT2|CYB5D_ARATH | Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1 | 10 | 106 | 5.0E-12 |
| sp|Q9V4N3|CYB5_DROME | Cytochrome b5 OS=Drosophila melanogaster GN=Cyt-b5 PE=2 SV=1 | 1 | 92 | 3.0E-11 |
| sp|O04354|CYB5_BOROF | Cytochrome b5 OS=Borago officinalis PE=2 SV=1 | 10 | 94 | 7.0E-11 |
| sp|P49099|CYB5S_TOBAC | Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1 | 10 | 94 | 9.0E-11 |
| sp|P49098|CYB5_TOBAC | Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1 | 10 | 86 | 1.0E-10 |
| sp|O48845|CYB5B_ARATH | Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1 | 10 | 94 | 2.0E-10 |
| sp|Q9USM6|CYB52_SCHPO | Probable cytochrome b5 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=oca8 PE=3 SV=1 | 8 | 111 | 2.0E-10 |
| sp|O94391|CYB51_SCHPO | Probable cytochrome b5 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC29A10.16c PE=3 SV=1 | 13 | 82 | 3.0E-10 |
| sp|P49097|CYB5_CUSRE | Cytochrome b5 OS=Cuscuta reflexa PE=2 SV=1 | 12 | 86 | 3.0E-10 |
| sp|Q42342|CYB5E_ARATH | Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2 | 3 | 101 | 5.0E-10 |
| sp|P49096|CYB5_MUSDO | Cytochrome b5 OS=Musca domestica GN=Cyt-b5 PE=1 SV=1 | 1 | 91 | 2.0E-09 |
| sp|P40934|CYB5_BRAOB | Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1 | 13 | 86 | 5.0E-09 |
| sp|P49100|CYB5_ORYSJ | Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2 | 1 | 84 | 7.0E-09 |
| sp|Q9FDW8|CYB5A_ARATH | Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1 | 6 | 82 | 2.0E-08 |
| sp|Q8X0J4|CYB5L_NEUCR | Putative cytochrome b5 B11H24.095 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=B11H24.095 PE=3 SV=1 | 8 | 55 | 4.0E-08 |
| sp|P36841|NIA_VOLCA | Nitrate reductase [NADH] OS=Volvox carteri GN=NITA PE=2 SV=1 | 10 | 117 | 4.0E-08 |
| sp|P39868|NIA2_BRANA | Nitrate reductase [NADH], clone PBNBR1412 OS=Brassica napus GN=NIA2 PE=2 SV=1 | 12 | 141 | 4.0E-08 |
| sp|Q9P5L0|CYB5_NEUCR | Probable cytochrome b5 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=B23L21.190 PE=3 SV=2 | 10 | 82 | 6.0E-08 |
| sp|Q9HFV1|CYB5_RHIST | Cytochrome b5 OS=Rhizopus stolonifer PE=2 SV=1 | 13 | 82 | 6.0E-08 |
| sp|Q9Y706|CYB5_MORAP | Cytochrome b5 OS=Mortierella alpina PE=1 SV=1 | 6 | 116 | 1.0E-07 |
| sp|P11832|NIA1_ARATH | Nitrate reductase [NADH] 1 OS=Arabidopsis thaliana GN=NIA1 PE=1 SV=3 | 12 | 141 | 1.0E-07 |
| sp|P39867|NIA1_BRANA | Nitrate reductase [NADH], clone PBNBR1405 OS=Brassica napus GN=NIA1 PE=2 SV=1 | 12 | 141 | 3.0E-07 |
| sp|P82291|CYB5_ECTSH | Soluble cytochrome b558 OS=Ectothiorhodospira shaposhnikovii PE=1 SV=2 | 5 | 82 | 3.0E-07 |
| sp|Q874I5|CYB5_CANTR | Cytochrome b5 OS=Candida tropicalis GN=Cytb5 PE=3 SV=1 | 10 | 92 | 4.0E-07 |
| sp|Q10352|YDAA_SCHPO | Uncharacterized protein C1F12.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC1F12.10c PE=3 SV=1 | 9 | 84 | 5.0E-07 |
| sp|Q9Y1W0|FAD5A_DICDI | Delta(5) fatty acid desaturase A OS=Dictyostelium discoideum GN=fadA PE=1 SV=1 | 12 | 86 | 5.0E-07 |
| sp|P32953|CYBL_RHOGR | (S)-mandelate dehydrogenase, mitochondrial OS=Rhodotorula graminis PE=1 SV=2 | 8 | 84 | 5.0E-07 |
| sp|P00172|CYB5_PIG | Cytochrome b5 OS=Sus scrofa GN=CYB5A PE=1 SV=3 | 10 | 99 | 7.0E-07 |
| sp|P49102|NIA3_MAIZE | Nitrate reductase [NADH] 3 OS=Zea mays PE=3 SV=1 | 3 | 141 | 9.0E-07 |
| sp|P00170|CYB5_HORSE | Cytochrome b5 OS=Equus caballus GN=CYB5A PE=1 SV=3 | 10 | 97 | 9.0E-07 |
| sp|P36859|NIA_PETHY | Nitrate reductase [NADH] OS=Petunia hybrida GN=NIA PE=2 SV=1 | 12 | 141 | 1.0E-06 |
| sp|P00171|CYB5_BOVIN | Cytochrome b5 OS=Bos taurus GN=CYB5A PE=1 SV=3 | 3 | 82 | 1.0E-06 |
| sp|P11035|NIA2_ARATH | Nitrate reductase [NADH] 2 OS=Arabidopsis thaliana GN=NIA2 PE=1 SV=1 | 5 | 82 | 1.0E-06 |
| sp|Q9CQX2|CYB5B_MOUSE | Cytochrome b5 type B OS=Mus musculus GN=Cyb5b PE=1 SV=1 | 13 | 84 | 1.0E-06 |
| sp|P00173|CYB5_RAT | Cytochrome b5 OS=Rattus norvegicus GN=Cyb5a PE=1 SV=2 | 10 | 99 | 1.0E-06 |
| sp|P27783|NIA_BETPN | Nitrate reductase [NAD(P)H] OS=Betula pendula GN=NIA1 PE=2 SV=1 | 12 | 116 | 1.0E-06 |
| sp|O22704|CYP5F_ARATH | Cytochrome B5-like protein OS=Arabidopsis thaliana GN=CB5LP PE=2 SV=1 | 8 | 82 | 2.0E-06 |
| sp|P39870|NIA2_SOYBN | Inducible nitrate reductase [NADH] 2 OS=Glycine max GN=INR2 PE=2 SV=1 | 5 | 141 | 2.0E-06 |
| sp|P56395|CYB5_MOUSE | Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2 | 10 | 97 | 2.0E-06 |
| sp|P04166|CYB5B_RAT | Cytochrome b5 type B OS=Rattus norvegicus GN=Cyb5b PE=1 SV=2 | 13 | 84 | 2.0E-06 |
| sp|Q01170|NIA_CHLVU | Nitrate reductase [NADH] (Fragment) OS=Chlorella vulgaris PE=2 SV=1 | 8 | 106 | 3.0E-06 |
| sp|Q5UR80|CYB5L_MIMIV | Cytochrome b5-like protein OS=Acanthamoeba polyphaga mimivirus GN=MIMI_L628 PE=3 SV=1 | 13 | 76 | 3.0E-06 |
| sp|P00174|CYB5_CHICK | Cytochrome b5 OS=Gallus gallus GN=CYB5A PE=1 SV=4 | 13 | 62 | 3.0E-06 |
| sp|Q5RDJ5|CYB5B_PONAB | Cytochrome b5 type B OS=Pongo abelii GN=CYB5B PE=2 SV=2 | 13 | 84 | 3.0E-06 |
| sp|P39869|NIA_LOTJA | Nitrate reductase [NADH] OS=Lotus japonicus GN=NIA PE=3 SV=1 | 12 | 82 | 4.0E-06 |
| sp|O43169|CYB5B_HUMAN | Cytochrome b5 type B OS=Homo sapiens GN=CYB5B PE=1 SV=2 | 13 | 84 | 4.0E-06 |
| sp|P00167|CYB5_HUMAN | Cytochrome b5 OS=Homo sapiens GN=CYB5A PE=1 SV=2 | 10 | 82 | 5.0E-06 |
| sp|P17569|NIA_CUCMA | Nitrate reductase [NADH] OS=Cucurbita maxima PE=2 SV=1 | 8 | 82 | 5.0E-06 |
| sp|P54233|NIA1_SOYBN | Inducible nitrate reductase [NADH] 1 OS=Glycine max GN=INR1 PE=2 SV=1 | 5 | 82 | 5.0E-06 |
| sp|P23312|NIA_SPIOL | Nitrate reductase [NADH] OS=Spinacia oleracea GN=NIA PE=2 SV=1 | 12 | 141 | 5.0E-06 |
| sp|Q9ZNV4|CYB5C_ARATH | Cytochrome B5 isoform C OS=Arabidopsis thaliana GN=CYTB5-C PE=1 SV=1 | 13 | 84 | 5.0E-06 |
| sp|P43101|NIA_CICIN | Nitrate reductase [NADH] OS=Cichorium intybus GN=NIA PE=2 SV=1 | 8 | 82 | 6.0E-06 |
| sp|P00175|CYB2_YEAST | Cytochrome b2, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CYB2 PE=1 SV=1 | 9 | 61 | 7.0E-06 |
| sp|P00168|CYB5_ALOSE | Cytochrome b5 (Fragment) OS=Alouatta seniculus GN=CYB5A PE=1 SV=2 | 10 | 82 | 7.0E-06 |
| sp|P09437|CYB2_WICAO | Cytochrome b2, mitochondrial OS=Wickerhamomyces anomalus GN=CYB2 PE=1 SV=2 | 9 | 59 | 9.0E-06 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0005506 | iron ion binding | Yes |
| GO:0016491 | oxidoreductase activity | Yes |
| GO:0008610 | lipid biosynthetic process | Yes |
| GO:0008152 | metabolic process | No |
| GO:0005488 | binding | No |
| GO:0009058 | biosynthetic process | No |
| GO:0071704 | organic substance metabolic process | No |
| GO:0046872 | metal ion binding | No |
| GO:0046914 | transition metal ion binding | No |
| GO:0044238 | primary metabolic process | No |
| GO:0006629 | lipid metabolic process | No |
| GO:0003824 | catalytic activity | No |
| GO:0003674 | molecular_function | No |
| GO:0043169 | cation binding | No |
| GO:0043167 | ion binding | No |
| GO:0008150 | biological_process | No |
| GO:1901576 | organic substance biosynthetic process | No |
| Localizations | Signals | Cytoplasm | Nucleus | Extracellular | Cell membrane | Mitochondrion | Plastid | Endoplasmic reticulum | Lysosome vacuole | Golgi apparatus | Peroxisome |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Endoplasmic reticulum | Signal peptide|Transmembrane domain | 0.1304 | 0.1721 | 0.0097 | 0.1072 | 0.168 | 0.1226 | 0.8711 | 0.5423 | 0.4635 | 0.0023 |
| Domain # | Start | End | Length |
|---|---|---|---|
| 1 | 191 | 210 | 19 |
| 2 | 217 | 239 | 22 |
| 3 | 277 | 299 | 22 |
| 4 | 306 | 328 | 22 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| SC16a | Pure fungal culture | 153.49 | 81.86 | 225.11 |
| CcL | In ants, during behavior modification | 87.11 | 46.84 | 127.38 |
| CcD | In ants, recently dead | 73.75 | 39.99 | 107.52 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| SC16a | CcL | 0.010753 | yes |
| SC16a | CcD | 0.000770 | yes |
| CcL | CcD | 0.503777 | no |
| Orthofinder run ID | 4 |
| Orthogroup | 4632 |
| Change Orthofinder run |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
Download genbank file of locus (reverse complement)
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Ophio5|6621 MPSRTLPTLTRAEVQSHDSTASCYVTIGSKVYDVTDFLSDHPGGAHLILEYAGKDVEAILQDPASHPHSEAAYEV LDDSLVGFVASPKAVVATPVTTGAVAAPGKSGSDPVPQVHPRTGMSCEGDLSKETDYSQDYKKHKFLDLSKPLFP QVWYGGFSKEFYLDQVHRPRHYKGGKSAPLFGNFLEPLTKTPWWLIPTLWLPCVAYGTYLASQGYYGPLATASFW AFGLFLWTLIEYGMHRFLFHVDDYLPDNRVGITAHFLLHGVHHYLPMDKYRLVMPPTLFVVLATPFWRLAHVLLS FDWHAATAVFCGGIFGYICYDLTHYFLHHQNLPLWYKQLKKYHLQHHFLDYELGFGVTSKFWDTVFGTELQPIKT SATNSIF |
| Coding | >Ophio5|6621 ATGCCTTCGAGGACGCTGCCGACGCTGACTCGCGCCGAGGTGCAATCGCACGACTCTACTGCCTCGTGCTACGTC ACCATTGGCTCCAAGGTCTACGATGTCACCGACTTCCTTTCCGACCACCCCGGCGGCGCCCATCTCATCCTAGAA TACGCCGGCAAGGATGTCGAGGCTATTCTTCAGGATCCCGCCTCGCACCCCCACTCCGAGGCCGCCTACGAGGTC CTCGACGACTCCTTGGTCGGCTTTGTCGCCTCGCCAAAGGCCGTCGTGGCCACTCCTGTCACCACTGGCGCCGTC GCGGCTCCCGGCAAGTCCGGCTCCGACCCCGTCCCCCAGGTCCACCCCCGCACCGGCATGTCGTGCGAGGGCGAC TTGTCCAAGGAGACCGATTACAGCCAGGACTACAAGAAGCACAAGTTTCTGGACCTTAGCAAGCCGCTCTTCCCT CAGGTCTGGTACGGCGGCTTCAGCAAAGAATTCTATCTCGACCAGGTCCACCGCCCGCGCCACTACAAGGGCGGC AAGTCGGCGCCTTTGTTTGGCAACTTCCTTGAGCCGCTCACCAAGACGCCGTGGTGGCTTATTCCTACTCTTTGG CTGCCCTGTGTCGCATACGGCACCTATCTCGCCTCCCAAGGCTACTACGGTCCCCTCGCCACGGCCTCCTTCTGG GCTTTCGGCCTGTTTTTGTGGACATTGATCGAGTACGGCATGCACCGGTTTCTCTTCCACGTCGATGACTATTTG CCCGACAACCGAGTCGGCATCACGGCGCACTTCTTGCTGCATGGCGTCCATCACTACCTCCCCATGGACAAATAC CGTCTGGTGATGCCGCCCACTCTTTTCGTCGTTCTTGCGACTCCCTTTTGGCGCCTGGCACATGTTCTCCTCTCC TTCGATTGGCACGCGGCAACGGCAGTCTTCTGCGGCGGCATCTTTGGCTACATCTGCTACGACCTGACGCACTAC TTTTTGCACCACCAGAACCTCCCGCTGTGGTACAAGCAGCTCAAGAAGTACCACTTGCAGCATCACTTTCTCGAT TACGAGCTAGGCTTCGGAGTCACGAGCAAATTCTGGGACACCGTCTTCGGCACAGAGCTGCAGCCCATTAAGACA AGTGCTACGAATAGCATATTT |
| Transcript | >Ophio5|6621 ATGCCTTCGAGGACGCTGCCGACGCTGACTCGCGCCGAGGTGCAATCGCACGACTCTACTGCCTCGTGCTACGTC ACCATTGGCTCCAAGGTCTACGATGTCACCGACTTCCTTTCCGACCACCCCGGCGGCGCCCATCTCATCCTAGAA TACGCCGGCAAGGATGTCGAGGCTATTCTTCAGGATCCCGCCTCGCACCCCCACTCCGAGGCCGCCTACGAGGTC CTCGACGACTCCTTGGTCGGCTTTGTCGCCTCGCCAAAGGCCGTCGTGGCCACTCCTGTCACCACTGGCGCCGTC GCGGCTCCCGGCAAGTCCGGCTCCGACCCCGTCCCCCAGGTCCACCCCCGCACCGGCATGTCGTGCGAGGGCGAC TTGTCCAAGGAGACCGATTACAGCCAGGACTACAAGAAGCACAAGTTTCTGGACCTTAGCAAGCCGCTCTTCCCT CAGGTCTGGTACGGCGGCTTCAGCAAAGAATTCTATCTCGACCAGGTCCACCGCCCGCGCCACTACAAGGGCGGC AAGTCGGCGCCTTTGTTTGGCAACTTCCTTGAGCCGCTCACCAAGACGCCGTGGTGGCTTATTCCTACTCTTTGG CTGCCCTGTGTCGCATACGGCACCTATCTCGCCTCCCAAGGCTACTACGGTCCCCTCGCCACGGCCTCCTTCTGG GCTTTCGGCCTGTTTTTGTGGACATTGATCGAGTACGGCATGCACCGGTTTCTCTTCCACGTCGATGACTATTTG CCCGACAACCGAGTCGGCATCACGGCGCACTTCTTGCTGCATGGCGTCCATCACTACCTCCCCATGGACAAATAC CGTCTGGTGATGCCGCCCACTCTTTTCGTCGTTCTTGCGACTCCCTTTTGGCGCCTGGCACATGTTCTCCTCTCC TTCGATTGGCACGCGGCAACGGCAGTCTTCTGCGGCGGCATCTTTGGCTACATCTGCTACGACCTGACGCACTAC TTTTTGCACCACCAGAACCTCCCGCTGTGGTACAAGCAGCTCAAGAAGTACCACTTGCAGCATCACTTTCTCGAT TACGAGCTAGGCTTCGGAGTCACGAGCAAATTCTGGGACACCGTCTTCGGCACAGAGCTGCAGCCCATTAAGACA AGTGCTACGAATAGCATATTTTAA |
| Gene | >Ophio5|6621 ATGCCTTCGAGGACGCTGCCGACGCTGACTCGCGCCGAGGTGCAATCGCACGACTCTACTGCCTCGTGCTACGTC ACCATTGGCTCCAAGGTCTACGATGTCACCGACTTCCTTTCCGACCACCCCGGCGGCGCCCATCTCATCCTAGAA TACGCCGGCAAGGATGTCGAGGCTATTCTTCAGGATCCCGCCTCGCACCCCCACTCCGAGGCCGCCTACGAGGTC CTCGACGACTCCTTGGTCGGCTTTGTCGCCTCGCCAAAGGCCGTCGTGGCCACTCCTGTCACCACTGGCGCCGTC GCGGCTCCCGGCAAGTCCGGCTCCGACCCCGTCCCCCAGGTCCACCCCCGCACCGGCATGTCGTGCGAGGGCGAC TTGTCCAAGGAGACCGATTACAGCCAGGACTACAAGAAGCACAAGTTTCTGGACCTTAGCAAGCCGCTCTTCCCT CAGGTCTGGTACGGCGGCTTCAGCAAAGAATTCTATCTCGACCAGGTCCACCGCCCGCGCCACTACAAGGGCGGC AAGTCGGCGCCTTTGTTTGGCAACTTCCTTGAGCCGCTCACCAAGACGCCGTGGTGGCTTATTCCTACTCTTTGG CTGCCCTGTGTCGCATACGGCACCTATCTCGCCTCCCAAGGCTACTACGGTCCCCTCGCCACGGCCTCCTTCTGG GCTTTCGGCCTGTTTTTGTGGACATTGATCGAGTACGGCATGCACCGGTTTCTCTTCCACGTCGATGAGTACGTC GAGGCTCGCTTGCACCTTTCGAGTCCCGCCTTTTGGCTGACCTTTGTCACAGCTATTTGCCCGACAACCGAGTCG GCATCACGGCGCACTTCTTGCTGCATGGCGTCCATCACTACCTCCCCATGGACAAATACCGTCTGGTGATGCCGC CCACTCTTTTCGTCGTTCTTGCGACTCCCTTTTGGCGCCTGGCACATGTTCTCCTCTCCTTCGATTGGCACGCGG CAACGGCAGTCTTCTGCGGCGGCATCTTTGGCTACATCTGCTACGACCTGACGCACTACTTTTTGCACCACCAGA ACCTCCCGCTGTGGTACAAGCAGCTCAAGAAGTACCACTTGCAGCATCACTTTCTCGATTACGAGCTAGGCTTCG GAGTCACGAGCAAATTCTGGGACACCGTCTTCGGCACAGAGCTGCAGCCCATTAAGACAAGGTAGCCCGCTTGCA TCTCTCTTTTGGTATAGCACGGCGTTGGGGCCTGTCCTTTGGGAGCGGGCATGTGAGCACGCAGCGGAGTCTTGT TCAGTTGCAGAGGCACAAAATTGTCTTGAACTGATGTTGTACAAACCTAGGTAGTGCTACGAATAGCATATTTTA A |