Fungal Genomics

at Utrecht University

General Properties

Protein IDOphio5|5003
Gene name
Locationscaffold_40:38747..39765
Strand-
Gene length (bp)1018
Transcript length (bp)846
Coding sequence length (bp)843
Protein length (aa) 281

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PFAM Domains

PFAM Domain ID Short name Long name E-value Start End
PF02544 Steroid_dh 3-oxo-5-alpha-steroid 4-dehydrogenase 1.6E-21 118 280
PF06966 DUF1295 Protein of unknown function (DUF1295) 1.7E-04 162 245

Swissprot hits

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Swissprot ID Swissprot Description Start End E-value
sp|Q9UT20|YFY8_SCHPO Uncharacterized protein C9.08c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC9.08c PE=3 SV=1 43 281 2.0E-35
sp|Q68FF9|S5A1_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2 25 281 3.0E-24
sp|P24008|S5A1_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2 51 281 5.0E-22
sp|A5PJS2|S5A1_BOVIN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus GN=SRD5A1 PE=2 SV=1 45 281 5.0E-21
sp|P18405|S5A1_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1 43 265 4.0E-19
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Swissprot ID Swissprot Description Start End E-value
sp|Q9UT20|YFY8_SCHPO Uncharacterized protein C9.08c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC9.08c PE=3 SV=1 43 281 2.0E-35
sp|Q68FF9|S5A1_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2 25 281 3.0E-24
sp|P24008|S5A1_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2 51 281 5.0E-22
sp|A5PJS2|S5A1_BOVIN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus GN=SRD5A1 PE=2 SV=1 45 281 5.0E-21
sp|P18405|S5A1_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1 43 265 4.0E-19
sp|Q5K2N1|DET2_SOLLC Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum GN=DET2 PE=1 SV=1 25 280 8.0E-19
sp|Q28891|S5A1_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1 43 265 1.0E-18
sp|P31213|S5A2_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens GN=SRD5A2 PE=1 SV=1 29 278 4.0E-17
sp|Q28892|S5A2_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV=1 29 278 1.0E-16
sp|O18765|S5A2_PIG 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa GN=SRD5A2 PE=2 SV=2 41 278 4.0E-16
sp|Q99N99|S5A2_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Mus musculus GN=Srd5a2 PE=2 SV=1 40 278 4.0E-15
sp|Q2QDF6|DET2_GOSHI Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1 16 278 9.0E-14
sp|Q38944|DET2_ARATH Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana GN=DET2 PE=1 SV=2 58 278 1.0E-13
sp|P31214|S5A2_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Rattus norvegicus GN=Srd5a2 PE=1 SV=1 40 278 3.0E-13
sp|Q7XUH5|POED1_ORYSJ Polyprenol reductase 1 OS=Oryza sativa subsp. japonica GN=Os04g0576800 PE=2 SV=2 181 278 5.0E-06
sp|A2XWN6|POED1_ORYSI Polyprenol reductase 1 OS=Oryza sativa subsp. indica GN=OsI_17065 PE=3 SV=1 181 278 5.0E-06
sp|Q7F0Q2|POED2_ORYSJ Polyprenol reductase 2 OS=Oryza sativa subsp. japonica GN=Os07g0493400 PE=2 SV=1 181 278 7.0E-06
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GO

GO Term Description Terminal node
GO:0006629 lipid metabolic process Yes
GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors Yes
GO:0071704 organic substance metabolic process No
GO:0016491 oxidoreductase activity No
GO:0044238 primary metabolic process No
GO:0003824 catalytic activity No
GO:0003674 molecular_function No
GO:0008152 metabolic process No
GO:0008150 biological_process No

SignalP

[Help with interpreting these statistics]
SignalP signal predicted Location
(based on Ymax)		 D score
(significance: > 0.45)
No 1 - 15 0.5

Transmembrane Domains

Domain # Start End Length
1 20 42 22
2 55 77 22
3 92 114 22
4 121 143 22
5 158 177 19
6 222 244 22

Transcription Factor Class

(None)

Expression data

Analysis 1: Expression analysis during behavioral modification. Published in De Bekker et al., 2017.

Expression values

Label Description Expression (RPKM) Confidence interval (low) Confidence interval (high)
SC16a Pure fungal culture 46.73 22.39 71.08
CcL In ants, during behavior modification 34.40 14.73 54.07
CcD In ants, recently dead 37.97 17.41 58.53

Differential expression

Label1 Label2 Q-value Significant difference
SC16a CcL 0.289154 no
SC16a CcD 0.479454 no
CcL CcD 0.763509 no

Sequences

Type of sequenceSequence
Locus Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein >Ophio5|5003
MALIQGWIPPSTRSRHETLLLFWQLAYPAIGSMQWLIKWYGMGKTSTPSRLNLPGRPAWLTMEVPGFLMLLYVMR
TMPAQHGIQDLPWQNQVLAGLFVIHYIYRAVLFPFLQPSMAPIHLFVWLLAISFQLINASCLGSWLAAYGPVTPT
AWAAQTPLPQFILGTVVFYLGLAANFFHDEELREIRRRRSNESGKNGRNSHYEIPQGGLFRYVLYPHYLCEWIEW
TGFLMAAGWTCAPAWAFLVNEVFSMLPRAVRGKAWYIDRFGRDKVAGRWAVIPGLL
Coding >Ophio5|5003
ATGGCCCTCATCCAGGGGTGGATCCCGCCTTCGACGCGCTCTCGCCACGAGACGCTCCTGTTATTCTGGCAGCTG
GCCTATCCCGCCATTGGCTCGATGCAGTGGCTCATCAAGTGGTACGGCATGGGCAAGACTTCAACGCCAAGCAGG
CTTAATCTTCCCGGCCGACCTGCGTGGTTGACGATGGAGGTGCCGGGTTTCTTGATGTTGTTGTATGTGATGCGT
ACGATGCCGGCGCAGCATGGGATACAGGACCTTCCGTGGCAGAATCAGGTCCTAGCCGGTTTATTTGTCATCCAC
TACATCTACCGCGCCGTCCTCTTTCCCTTCCTCCAACCCTCCATGGCCCCGATTCATCTCTTCGTCTGGCTCTTG
GCCATCAGCTTCCAACTCATAAATGCGAGCTGTCTAGGCTCCTGGCTCGCCGCCTACGGACCAGTCACGCCAACC
GCCTGGGCCGCGCAGACGCCCCTTCCGCAATTCATACTCGGCACCGTCGTATTTTACCTTGGCCTTGCCGCCAAC
TTCTTTCACGATGAGGAACTGCGTGAGATTCGTCGTCGTCGCTCAAATGAGAGTGGAAAGAATGGCAGAAATAGC
CACTACGAGATCCCGCAGGGTGGCCTCTTTCGCTACGTCCTCTATCCGCATTATCTCTGCGAGTGGATTGAGTGG
ACTGGCTTCCTTATGGCTGCTGGTTGGACATGCGCGCCCGCCTGGGCCTTTTTGGTCAACGAGGTGTTTTCCATG
CTGCCGCGTGCCGTCCGTGGCAAGGCTTGGTATATCGACCGCTTTGGCCGTGACAAGGTAGCCGGTCGCTGGGCC
GTCATCCCGGGGCTGCTT
Transcript >Ophio5|5003
ATGGCCCTCATCCAGGGGTGGATCCCGCCTTCGACGCGCTCTCGCCACGAGACGCTCCTGTTATTCTGGCAGCTG
GCCTATCCCGCCATTGGCTCGATGCAGTGGCTCATCAAGTGGTACGGCATGGGCAAGACTTCAACGCCAAGCAGG
CTTAATCTTCCCGGCCGACCTGCGTGGTTGACGATGGAGGTGCCGGGTTTCTTGATGTTGTTGTATGTGATGCGT
ACGATGCCGGCGCAGCATGGGATACAGGACCTTCCGTGGCAGAATCAGGTCCTAGCCGGTTTATTTGTCATCCAC
TACATCTACCGCGCCGTCCTCTTTCCCTTCCTCCAACCCTCCATGGCCCCGATTCATCTCTTCGTCTGGCTCTTG
GCCATCAGCTTCCAACTCATAAATGCGAGCTGTCTAGGCTCCTGGCTCGCCGCCTACGGACCAGTCACGCCAACC
GCCTGGGCCGCGCAGACGCCCCTTCCGCAATTCATACTCGGCACCGTCGTATTTTACCTTGGCCTTGCCGCCAAC
TTCTTTCACGATGAGGAACTGCGTGAGATTCGTCGTCGTCGCTCAAATGAGAGTGGAAAGAATGGCAGAAATAGC
CACTACGAGATCCCGCAGGGTGGCCTCTTTCGCTACGTCCTCTATCCGCATTATCTCTGCGAGTGGATTGAGTGG
ACTGGCTTCCTTATGGCTGCTGGTTGGACATGCGCGCCCGCCTGGGCCTTTTTGGTCAACGAGGTGTTTTCCATG
CTGCCGCGTGCCGTCCGTGGCAAGGCTTGGTATATCGACCGCTTTGGCCGTGACAAGGTAGCCGGTCGCTGGGCC
GTCATCCCGGGGCTGCTTTGA
Gene >Ophio5|5003
ATGGCCCTCATCCAGGGGTGGATCCCGCCTTCGACGCGCTCTCGCCACGAGACGCTCCTGTTATTCTGGCAGCTG
GCCTATCCCGCCGCAAGTCTCTCTCCCTAATTCGTGAAGGCGAATCCGCCACTAATGCCGGGCCTTTTTTTTTCT
CTTTTTGTCGGTGATCTTGTACAGATTGGCTCGATGCAGTGGCTCATCAAGTGGTACGGCATGGGCAAGACTTCA
ACGCCAAGCAGGCTTAATCTTCCCGGCCGACCTGCGTGGTTGACGATGGAGGTGCCGGGTTTCTTGATGTTGTTG
TATGTGATGCGTACGATGCCGGCGCAGCATGGGATACAGGACCTTCCGTGGCAGAATCAGGTCCTAGCCGGTTTA
TTTGTTCGTCTTTCTCTCATCTCTCTCTCTCTTTCTCTTCTTGCCACTCTTGTCATTCGCTGACCAAAGCCCTCC
CCCTTCAAAATAGGTCATCCACTACATCTACCGCGCCGTCCTCTTTCCCTTCCTCCAACCCTCCATGGCCCCGAT
TCATCTCTTCGTCTGGCTCTTGGCCATCAGCTTCCAACTCATAAATGCGAGCTGTCTAGGCTCCTGGCTCGCCGC
CTACGGACCAGTCACGCCAACCGCCTGGGCCGCGCAGACGCCCCTTCCGCAATTCATACTCGGCACCGTCGTATT
TTACCTTGGCCTTGCCGCCAACTTCTTTCACGATGAGGAACTGCGTGAGATTCGTCGTCGTCGCTCAAATGAGAG
TGGAAAGAATGGCAGAAATAGCCACTACGAGATCCCGCAGGGTGGCCTCTTTCGCTACGTCCTCTATCCGCATTA
TCTCTGCGAGTGGATTGAGTGGACTGGCTTCCTTATGGCTGCTGGTTGGACATGCGCGCCCGCCTGGGCCTTTTT
GGTCAACGAGGTGTTTTCCATGCTGCCGCGTGCCGTCCGTGGCAAGGCTTGGTATATCGACCGCTTTGGCCGTGA
CAAGGTAGCCGGTCGCTGGGCCGTCATCCCGGGGCTGCTTTGA

© 2022 - Robin Ohm - Utrecht University - The Netherlands

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