Fungal Genomics

at Utrecht University

General Properties

Protein ID	Ophio5\|4338
Gene name
Location	scaffold_343:1892..5231
Strand	+
Gene length (bp)	3339
Transcript length (bp)	3270
Coding sequence length (bp)	3267
Protein length (aa)	1089

PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF01764	Lipase_3	Lipase (class 3)	2.6E-15	805	965

Swissprot hits

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Swissprot ID	Swissprot Description	Start	End	E-value
sp\|Q5YLM1\|DGLA_RAT	Sn1-specific diacylglycerol lipase alpha OS=Rattus norvegicus GN=Dagla PE=1 SV=1	794	968	7.0E-10
sp\|Q6WQJ1\|DGLA_MOUSE	Sn1-specific diacylglycerol lipase alpha OS=Mus musculus GN=Dagla PE=1 SV=2	794	968	7.0E-10
sp\|Q9Y4D2\|DGLA_HUMAN	Sn1-specific diacylglycerol lipase alpha OS=Homo sapiens GN=DAGLA PE=1 SV=3	794	968	3.0E-09
sp\|P0C1S9\|DGLB_RAT	Sn1-specific diacylglycerol lipase beta OS=Rattus norvegicus GN=Daglb PE=1 SV=1	795	970	9.0E-09
sp\|Q8NCG7\|DGLB_HUMAN	Sn1-specific diacylglycerol lipase beta OS=Homo sapiens GN=DAGLB PE=1 SV=2	795	970	1.0E-08

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Swissprot ID	Swissprot Description	Start	End	E-value
sp\|Q5YLM1\|DGLA_RAT	Sn1-specific diacylglycerol lipase alpha OS=Rattus norvegicus GN=Dagla PE=1 SV=1	794	968	7.0E-10
sp\|Q6WQJ1\|DGLA_MOUSE	Sn1-specific diacylglycerol lipase alpha OS=Mus musculus GN=Dagla PE=1 SV=2	794	968	7.0E-10
sp\|Q9Y4D2\|DGLA_HUMAN	Sn1-specific diacylglycerol lipase alpha OS=Homo sapiens GN=DAGLA PE=1 SV=3	794	968	3.0E-09
sp\|P0C1S9\|DGLB_RAT	Sn1-specific diacylglycerol lipase beta OS=Rattus norvegicus GN=Daglb PE=1 SV=1	795	970	9.0E-09
sp\|Q8NCG7\|DGLB_HUMAN	Sn1-specific diacylglycerol lipase beta OS=Homo sapiens GN=DAGLB PE=1 SV=2	795	970	1.0E-08
sp\|Q91WC9\|DGLB_MOUSE	Sn1-specific diacylglycerol lipase beta OS=Mus musculus GN=Daglb PE=1 SV=2	795	970	2.0E-08
sp\|P61872\|LIP_RHIOR	Lipase OS=Rhizopus oryzae PE=1 SV=1	794	969	7.0E-07
sp\|P61871\|LIP_RHINI	Lipase OS=Rhizopus niveus PE=1 SV=1	794	969	7.0E-07

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GO

GO Term	Description	Terminal node
GO:0006629	lipid metabolic process	Yes
GO:0071704	organic substance metabolic process	No
GO:0044238	primary metabolic process	No
GO:0008152	metabolic process	No
GO:0008150	biological_process	No

SignalP

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SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
No	1 - 51	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Expression analysis during behavioral modification. Published in De Bekker et al., 2017.

Expression values

Label	Description	Expression (RPKM)	Confidence interval (low)	Confidence interval (high)
SC16a	Pure fungal culture	35.13	19.14	51.11
CcL	In ants, during behavior modification	82.88	37.68	128.08
CcD	In ants, recently dead	39.47	21.26	57.69

Differential expression

Label1	Label2	Q-value	Significant difference
SC16a	CcL	0.000535	yes
SC16a	CcD	0.651656	no
CcL	CcD	0.003573	yes

Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>Ophio5\|4338 MAAREEPYLDETLPTTTTHRSVSVVPPAPTLLPRPVAQAVSLATRSTGLAIRFGSLVSSYGLDAARFTTLSSLEL ARGLVEGVLSRAARDTLARSNSDLAAADAETILERSLESLHYAVAQIVFWTSASFRLTGVTLSAASEASQQLLSS LDQLFGSTDSSRAVASLVTLIRREFSDPATGVGGEKVGVIDLILALSALAYLQRACRTCPAEESRRQACEELIWD VVVLCDGQRVDVEEDTPSTSFVPLRGSDVSDDNDNEAILTSLKDHVVNSLPPGTAVTISDCVSSVQTITVDVEGS QLPSLPTPPGAEIIETRGLSPASQRPLTPLSGCAHDPSYRVVYRIQRERFRSTELRHEDETSGPVVIELCDEKPP AWPASPIDEKPRPSGETLPSTGQHHGGDGDETVKGVVSDRPAATKATTPEKASTVTAPSTSTATTPKTATTPTTA AGDKPQRPPLRPARVSSSDITANQKKQRLPLGREDTVKADAGKRSQIDVGKRPQIKKKTDTGIASKPAEKKNGLK HLLKDRGQSVARIWSRDQAEASPTPPKPRPVRKVSGGGVSAVGDKPKSAPPRTSIPAAQRNLALARRPPSQGSVA TTSTTASAKLLSIQERRRDSVVSLADTFVQHADGGLRPATPTVLRADFSSSGPASPAPYTASLYSMATNNDSQSS LVLASCYQKSAYHESGALSTLRREGFVDGAFPGGPLLRNMARYMRFSSACYGSHFLRFMGISREVPALGVWDGTH HDVRHFVHHTESDASSILLASFVDPQGGADASGATGTGVPLVHYISLDHAAKAVVLACRGTLGFEDVMADLACDY DDLKWRGRRYKVHKGIHASARRLLYGGDGRVLVTIREALLAFPDYGLVLCGHSLGGGVTSLLGVMLARPNPDGPG FVIAAAPSSRDAAPALEGLRLPPGRRIHVYAYGPPGVMSAALRQITRGLVTSVVHGHDLVPHLSFGLLHDCQALA HTFKYDESGAKADLRLRLWNALADRWRHRPRAPAEADQLWMLPALQTLRRSLRAVKLLPPGEVFAIESRRVLRRD AFVLRDGDGAGVGRPAQRVMLSPAEYEDALNMLRLGVIE
Coding	>Ophio5\|4338 ATGGCCGCTCGCGAGGAGCCTTATCTGGACGAGACCCTACCCACAACGACAACGCACCGTTCAGTCTCCGTTGTC CCGCCGGCCCCGACGCTTCTGCCGCGCCCCGTCGCCCAGGCTGTCAGTCTAGCGACACGCTCGACGGGCCTCGCT ATCCGATTCGGCTCGCTCGTCTCATCGTATGGCCTCGATGCTGCCCGCTTCACGACCCTTTCCAGCCTCGAGCTA GCCCGCGGCCTGGTAGAGGGCGTCCTCAGCCGCGCCGCGCGGGATACGCTGGCCCGCTCCAACTCGGACCTCGCC GCCGCCGATGCTGAGACGATTCTCGAGCGCAGCCTCGAGAGCTTGCACTATGCCGTCGCTCAGATCGTCTTCTGG ACTTCGGCCAGCTTTCGTCTAACGGGCGTTACCCTCTCCGCTGCCAGCGAGGCCTCGCAGCAGCTGCTGTCGTCT CTCGACCAGCTCTTCGGCTCGACCGACTCTTCCCGCGCCGTCGCCAGCCTCGTCACCCTCATCCGCCGCGAGTTC AGTGATCCGGCGACAGGCGTTGGCGGCGAAAAGGTCGGCGTCATCGACCTCATACTCGCTCTCAGCGCCCTGGCC TACCTCCAGCGTGCCTGTCGCACATGCCCCGCCGAGGAGAGCCGACGCCAGGCGTGCGAAGAGCTCATCTGGGAC GTCGTTGTCCTCTGCGACGGCCAGCGCGTTGATGTTGAAGAGGACACGCCGTCGACATCGTTCGTGCCTCTCCGA GGGTCAGACGTCAGCGACGACAACGACAACGAGGCCATCCTCACCAGTCTCAAGGACCACGTCGTCAACTCGCTA CCTCCGGGCACCGCTGTCACCATCTCTGACTGCGTATCCTCGGTGCAGACCATCACCGTCGACGTCGAGGGCTCT CAGCTGCCGTCGCTGCCGACGCCCCCTGGCGCCGAGATAATCGAGACGCGGGGGCTGTCGCCTGCAAGCCAACGG CCGCTGACGCCTCTGTCTGGCTGCGCGCACGACCCGTCGTATCGTGTCGTCTACAGGATTCAGCGTGAGCGGTTC CGGTCGACGGAGCTGCGGCACGAGGACGAGACGTCTGGACCGGTCGTCATCGAGCTGTGTGACGAAAAGCCGCCC GCATGGCCAGCGAGCCCGATTGATGAGAAGCCGAGGCCGTCGGGCGAGACTCTGCCGTCGACAGGCCAGCACCAT GGAGGCGACGGCGACGAGACAGTCAAGGGGGTTGTCTCGGATCGGCCCGCGGCCACGAAGGCTACGACGCCTGAG AAGGCCTCTACCGTCACCGCGCCTTCGACATCTACGGCCACGACGCCAAAAACGGCCACGACGCCTACGACAGCC GCCGGCGACAAGCCTCAACGACCGCCGCTACGGCCGGCACGAGTCTCGTCTTCCGATATTACAGCGAACCAAAAG AAGCAGCGCTTGCCGCTCGGGCGCGAGGACACGGTCAAGGCGGATGCCGGCAAGCGCTCTCAGATAGATGTTGGC AAACGCCCCCAAATCAAGAAGAAGACGGACACAGGCATAGCGTCAAAGCCGGCAGAGAAGAAGAACGGACTGAAG CACCTCCTCAAGGACAGGGGCCAGTCTGTGGCCAGGATCTGGAGTAGGGACCAGGCCGAGGCAAGCCCAACGCCA CCAAAGCCACGGCCGGTCCGTAAAGTCTCCGGCGGCGGCGTGTCGGCGGTTGGCGACAAGCCGAAGTCGGCCCCG CCTAGAACCTCGATTCCAGCGGCGCAGCGAAACCTGGCCTTGGCTCGTCGGCCGCCATCTCAGGGGTCGGTTGCC ACAACCAGCACGACGGCCAGCGCCAAGTTGTTGTCGATACAGGAACGACGGCGCGACTCGGTCGTCTCGCTGGCC GACACCTTCGTCCAGCACGCCGACGGGGGGCTCCGACCGGCGACGCCGACGGTGCTTCGGGCCGACTTCTCCAGC AGCGGGCCGGCATCGCCAGCGCCGTACACGGCCAGCCTTTACAGCATGGCCACGAACAACGACTCGCAGTCGTCG CTGGTGTTGGCGTCGTGCTACCAGAAGAGCGCCTATCACGAGTCCGGGGCACTCAGCACACTGCGGCGCGAGGGC TTCGTTGACGGCGCGTTCCCCGGTGGGCCGCTGCTGCGCAACATGGCACGCTACATGCGCTTCTCGTCGGCCTGT TACGGCTCGCATTTCCTGCGCTTCATGGGCATCTCGCGCGAGGTGCCGGCGCTCGGCGTCTGGGACGGCACTCAC CACGACGTGAGGCATTTTGTTCACCACACTGAGAGCGATGCTAGCAGCATCCTGCTGGCGTCATTTGTCGATCCA CAGGGCGGCGCAGACGCGTCTGGCGCGACAGGCACGGGCGTGCCGCTGGTACACTACATCTCTCTCGATCATGCG GCCAAGGCCGTGGTACTGGCCTGTCGCGGCACGCTGGGCTTCGAGGACGTCATGGCCGACCTGGCCTGCGATTAC GACGATCTCAAGTGGCGCGGCCGCCGGTACAAGGTGCACAAGGGCATCCACGCGTCTGCGCGTCGGTTGCTGTAT GGAGGCGACGGACGCGTCCTCGTCACCATCCGCGAGGCGCTGCTGGCCTTTCCCGACTATGGCCTCGTGCTGTGC GGCCACTCGCTCGGAGGCGGCGTCACCTCGCTACTGGGCGTCATGCTGGCCCGGCCTAACCCGGACGGACCGGGC TTCGTCATCGCCGCCGCGCCTTCGAGCCGCGATGCCGCGCCTGCGCTCGAAGGCCTACGACTCCCTCCCGGCCGA CGAATCCACGTCTACGCCTACGGCCCGCCGGGCGTCATGTCTGCGGCGCTACGTCAAATCACGCGCGGCCTCGTC ACCAGCGTCGTCCACGGCCACGACCTCGTCCCCCACCTCTCATTCGGCCTCCTTCACGACTGCCAAGCGCTGGCG CACACCTTCAAGTACGATGAGAGCGGCGCGAAAGCCGACCTGCGTCTCCGACTGTGGAACGCCCTCGCCGACCGT TGGAGGCATCGGCCCCGAGCGCCGGCCGAGGCGGATCAGCTGTGGATGCTGCCGGCGCTGCAGACGCTGCGGCGG TCCCTCCGCGCCGTGAAGCTGCTCCCGCCCGGGGAGGTGTTTGCCATCGAGAGCCGTCGCGTGCTGCGTCGCGAC GCTTTTGTCCTTCGCGACGGTGACGGGGCTGGCGTTGGACGGCCGGCCCAGAGGGTCATGCTCAGCCCCGCCGAG TACGAGGATGCGCTGAATATGTTGCGGTTGGGGGTTATTGAG
Transcript	>Ophio5\|4338 ATGGCCGCTCGCGAGGAGCCTTATCTGGACGAGACCCTACCCACAACGACAACGCACCGTTCAGTCTCCGTTGTC CCGCCGGCCCCGACGCTTCTGCCGCGCCCCGTCGCCCAGGCTGTCAGTCTAGCGACACGCTCGACGGGCCTCGCT ATCCGATTCGGCTCGCTCGTCTCATCGTATGGCCTCGATGCTGCCCGCTTCACGACCCTTTCCAGCCTCGAGCTA GCCCGCGGCCTGGTAGAGGGCGTCCTCAGCCGCGCCGCGCGGGATACGCTGGCCCGCTCCAACTCGGACCTCGCC GCCGCCGATGCTGAGACGATTCTCGAGCGCAGCCTCGAGAGCTTGCACTATGCCGTCGCTCAGATCGTCTTCTGG ACTTCGGCCAGCTTTCGTCTAACGGGCGTTACCCTCTCCGCTGCCAGCGAGGCCTCGCAGCAGCTGCTGTCGTCT CTCGACCAGCTCTTCGGCTCGACCGACTCTTCCCGCGCCGTCGCCAGCCTCGTCACCCTCATCCGCCGCGAGTTC AGTGATCCGGCGACAGGCGTTGGCGGCGAAAAGGTCGGCGTCATCGACCTCATACTCGCTCTCAGCGCCCTGGCC TACCTCCAGCGTGCCTGTCGCACATGCCCCGCCGAGGAGAGCCGACGCCAGGCGTGCGAAGAGCTCATCTGGGAC GTCGTTGTCCTCTGCGACGGCCAGCGCGTTGATGTTGAAGAGGACACGCCGTCGACATCGTTCGTGCCTCTCCGA GGGTCAGACGTCAGCGACGACAACGACAACGAGGCCATCCTCACCAGTCTCAAGGACCACGTCGTCAACTCGCTA CCTCCGGGCACCGCTGTCACCATCTCTGACTGCGTATCCTCGGTGCAGACCATCACCGTCGACGTCGAGGGCTCT CAGCTGCCGTCGCTGCCGACGCCCCCTGGCGCCGAGATAATCGAGACGCGGGGGCTGTCGCCTGCAAGCCAACGG CCGCTGACGCCTCTGTCTGGCTGCGCGCACGACCCGTCGTATCGTGTCGTCTACAGGATTCAGCGTGAGCGGTTC CGGTCGACGGAGCTGCGGCACGAGGACGAGACGTCTGGACCGGTCGTCATCGAGCTGTGTGACGAAAAGCCGCCC GCATGGCCAGCGAGCCCGATTGATGAGAAGCCGAGGCCGTCGGGCGAGACTCTGCCGTCGACAGGCCAGCACCAT GGAGGCGACGGCGACGAGACAGTCAAGGGGGTTGTCTCGGATCGGCCCGCGGCCACGAAGGCTACGACGCCTGAG AAGGCCTCTACCGTCACCGCGCCTTCGACATCTACGGCCACGACGCCAAAAACGGCCACGACGCCTACGACAGCC GCCGGCGACAAGCCTCAACGACCGCCGCTACGGCCGGCACGAGTCTCGTCTTCCGATATTACAGCGAACCAAAAG AAGCAGCGCTTGCCGCTCGGGCGCGAGGACACGGTCAAGGCGGATGCCGGCAAGCGCTCTCAGATAGATGTTGGC AAACGCCCCCAAATCAAGAAGAAGACGGACACAGGCATAGCGTCAAAGCCGGCAGAGAAGAAGAACGGACTGAAG CACCTCCTCAAGGACAGGGGCCAGTCTGTGGCCAGGATCTGGAGTAGGGACCAGGCCGAGGCAAGCCCAACGCCA CCAAAGCCACGGCCGGTCCGTAAAGTCTCCGGCGGCGGCGTGTCGGCGGTTGGCGACAAGCCGAAGTCGGCCCCG CCTAGAACCTCGATTCCAGCGGCGCAGCGAAACCTGGCCTTGGCTCGTCGGCCGCCATCTCAGGGGTCGGTTGCC ACAACCAGCACGACGGCCAGCGCCAAGTTGTTGTCGATACAGGAACGACGGCGCGACTCGGTCGTCTCGCTGGCC GACACCTTCGTCCAGCACGCCGACGGGGGGCTCCGACCGGCGACGCCGACGGTGCTTCGGGCCGACTTCTCCAGC AGCGGGCCGGCATCGCCAGCGCCGTACACGGCCAGCCTTTACAGCATGGCCACGAACAACGACTCGCAGTCGTCG CTGGTGTTGGCGTCGTGCTACCAGAAGAGCGCCTATCACGAGTCCGGGGCACTCAGCACACTGCGGCGCGAGGGC TTCGTTGACGGCGCGTTCCCCGGTGGGCCGCTGCTGCGCAACATGGCACGCTACATGCGCTTCTCGTCGGCCTGT TACGGCTCGCATTTCCTGCGCTTCATGGGCATCTCGCGCGAGGTGCCGGCGCTCGGCGTCTGGGACGGCACTCAC CACGACGTGAGGCATTTTGTTCACCACACTGAGAGCGATGCTAGCAGCATCCTGCTGGCGTCATTTGTCGATCCA CAGGGCGGCGCAGACGCGTCTGGCGCGACAGGCACGGGCGTGCCGCTGGTACACTACATCTCTCTCGATCATGCG GCCAAGGCCGTGGTACTGGCCTGTCGCGGCACGCTGGGCTTCGAGGACGTCATGGCCGACCTGGCCTGCGATTAC GACGATCTCAAGTGGCGCGGCCGCCGGTACAAGGTGCACAAGGGCATCCACGCGTCTGCGCGTCGGTTGCTGTAT GGAGGCGACGGACGCGTCCTCGTCACCATCCGCGAGGCGCTGCTGGCCTTTCCCGACTATGGCCTCGTGCTGTGC GGCCACTCGCTCGGAGGCGGCGTCACCTCGCTACTGGGCGTCATGCTGGCCCGGCCTAACCCGGACGGACCGGGC TTCGTCATCGCCGCCGCGCCTTCGAGCCGCGATGCCGCGCCTGCGCTCGAAGGCCTACGACTCCCTCCCGGCCGA CGAATCCACGTCTACGCCTACGGCCCGCCGGGCGTCATGTCTGCGGCGCTACGTCAAATCACGCGCGGCCTCGTC ACCAGCGTCGTCCACGGCCACGACCTCGTCCCCCACCTCTCATTCGGCCTCCTTCACGACTGCCAAGCGCTGGCG CACACCTTCAAGTACGATGAGAGCGGCGCGAAAGCCGACCTGCGTCTCCGACTGTGGAACGCCCTCGCCGACCGT TGGAGGCATCGGCCCCGAGCGCCGGCCGAGGCGGATCAGCTGTGGATGCTGCCGGCGCTGCAGACGCTGCGGCGG TCCCTCCGCGCCGTGAAGCTGCTCCCGCCCGGGGAGGTGTTTGCCATCGAGAGCCGTCGCGTGCTGCGTCGCGAC GCTTTTGTCCTTCGCGACGGTGACGGGGCTGGCGTTGGACGGCCGGCCCAGAGGGTCATGCTCAGCCCCGCCGAG TACGAGGATGCGCTGAATATGTTGCGGTTGGGGGTTATTGAGTAG
Gene	>Ophio5\|4338 ATGGCCGCTCGCGAGGAGCCTTATCTGGACGAGACCCTACCCACAACGACAACGCACCGTTCAGTCTCCGTTGTC CCGCCGGCCCCGACGCTTCTGCCGCGCCCCGTCGCCCAGGCTGTCAGTCTAGCGACACGCTCGACGGGCCTCGCT ATCCGATTCGGCTCGCTCGTCTCATCGTATGGCCTCGATGCTGCCCGCTTCACGACCCTTTCCAGCCTCGAGCTA GCCCGCGGCCTGGTAGAGGGCGTCCTCAGCCGCGCCGCGCGGGATACGCTGGCCCGCTCCAACTCGGACCTCGCC GCCGCCGATGCTGAGACGATTCTCGAGCGCAGCCTCGAGAGCTTGCACTATGCCGTCGCTCAGATCGTCTTCTGG ACTTCGGCCAGCTTTCGTCTAACGGGCGTTACCCTCTCCGCTGCCAGCGAGGCCTCGCAGCAGCTGCTGTCGTCT CTCGACCAGCTCTTCGGCTCGACCGACTCTTCCCGCGCCGTCGCCAGCCTCGTCACCCTCATCCGCCGCGAGTTC AGTGATCCGGCGACAGGCGTTGGCGGCGAAAAGGTCGGCGTCATCGACCTCATACTCGCTCTCAGCGCCCTGGCC TACCTCCAGCGTGCCTGTCGCACATGCCCCGCCGAGGAGAGCCGACGCCAGGCGTGCGAAGAGCTCATCTGGGAC GTCGTTGTCCTCTGCGACGGCCAGCGCGTTGATGTTGAAGAGGACACGCCGTCGACATCGTTCGTGCCTCTCCGA GGGTCAGACGTCAGCGACGACAACGACAACGAGGCCATCCTCACCAGTCTCAAGGACCACGTCGTCAACTCGCTA CCTCCGGGCACCGCTGTCACCATCTCTGACTGCGTATCCTCGGTGCAGACCATCACCGTCGACGTCGAGGGCTCT CAGCTGCCGTCGCTGCCGACGCCCCCTGGCGCCGAGATAATCGAGACGCGGGGGCTGTCGCCTGCAAGCCAACGG CCGCTGACGCCTCTGTCTGGCTGCGCGCACGACCCGTCGTATCGTGTCGTCTACAGGATTCAGCGTGAGCGGTTC CGGTCGACGGAGCTGCGGCACGAGGACGAGACGTCTGGACCGGTCGTCATCGAGCTGTGTGACGAAAAGCCGCCC GCATGGCCAGCGAGCCCGATTGATGAGAAGCCGAGGCCGTCGGGCGAGACTCTGCCGTCGACAGGCCAGCACCAT GGAGGCGACGGCGACGAGACAGTCAAGGGGGTTGTCTCGGATCGGCCCGCGGCCACGAAGGCTACGACGCCTGAG AAGGCCTCTACCGTCACCGCGCCTTCGACATCTACGGCCACGACGCCAAAAACGGCCACGACGCCTACGACAGCC GCCGGCGACAAGCCTCAACGACCGCCGCTACGGCCGGCACGAGTCTCGTCTTCCGATATTACAGCGAACCAAAAG AAGCAGCGCTTGCCGCTCGGGCGCGAGGACACGGTCAAGGCGGATGCCGGCAAGCGCTCTCAGATAGATGTTGGC AAACGCCCCCAAATCAAGAAGAAGACGGACACAGGCATAGCGTCAAAGCCGGCAGAGAAGAAGAACGGACTGAAG CACCTCCTCAAGGACAGGGGCCAGTCTGTGGCCAGGATCTGGAGTAGGGACCAGGCCGAGGCAAGCCCAACGCCA CCAAAGCCACGGCCGGTCCGTAAAGTCTCCGGCGGCGGCGTGTCGGCGGTTGGCGACAAGCCGAAGTCGGCCCCG CCTAGAACCTCGATTCCAGCGGCGCAGCGAAACCTGGCCTTGGCTCGTCGGCCGCCATCTCAGGGGTCGGTTGCC ACAACCAGCACGACGGCCAGCGCCAAGTTGTTGTCGATACAGGAACGACGGCGCGACTCGGTCGTCTCGCTGGCC GACACCTTCGTCCAGCACGCCGACGGGGGGCTCCGACCGGCGACGCCGACGGTGCTTCGGGCCGACTTCTCCAGC AGCGGGCCGGCATCGCCAGCGCCGTACACGGCCAGCCTTTACAGCATGGCCACGAACAACGACTCGCAGTCGTCG CTGGTGTTGGCGTCGTGCTACCAGAAGAGCGCCTATCACGAGTCCGGGGCACTCAGCACACTGCGGCGCGAGGGC TTCGTTGACGGCGCGTTCCCCGGTGGGCCGCTGCTGCGCAACATGGCACGCTACATGCGCTTCTCGTCGGCCTGT TACGGCTCGCATTTCCTGCGCTTCATGGGCATCTCGCGCGAGGTGCCGGCGCTCGGCGTCTGGGACGGCACTCAC CACGACGTGAGGCATTTTGTTCACCACACTGAGAGCGATGCTAGCAGCATCCTGCTGGCGTCATTTGTCGATCCA CAGGGCGGCGCAGACGCGTCTGGCGCGACAGGCACGGGCGTGCCGCTGGTACACTACATCTCTCTCGATCATGCG GCCAAGGCCGTGGTACTGGCCTGTCGCGGCACGCTGGGCTTCGAGGACGTCATGGCCGACCTGGCCTGCGATTAC GACGATCTCAAGTGGCGCGGCCGCCGGTACAAGGTGCACAAGGGCATCCACGCGTCTGCGCGTCGGTTGCTGTAT GGAGGCGACGGACGCGTCCTCGTCACCATCCGCGAGGCGCTGCTGGCCTTTCCCGACTATGGCCTCGTGCTGTGC GGCCACTCGCTCGGAGGCGGCGTCACCTCGCTACTGGGCGTCATGCTGGCCCGGCCTAACCCGGACGGACCGGGC TTCGTCATCGCCGCCGCGCCTTCGAGCCGCGATGCCGCGCCTGCGCTCGAAGGCCTACGACTCCCTCCCGGCCGA CGAATCCACGTCTACGCCTACGGCCCGCCGGGCGTCATGTCTGCGGCGCTACGTCAAATCACGCGCGGCCTCGTC ACCAGCGTCGTCCACGGCCACGACCTCGTCCCCCACCTCTCATTCGGCCTCCTTCACGACTGCCAAGCGCTGGCG CACACCTTCAAGTACGATGAGAGCGGCGCGAAAGCCGACCTGCGTCTCCGACTGTGGAACGCCCTCGCCGACCGT TGGAGGCATCGGCCCCGAGCGCCGGCCGAGGCGGATCAGCTGTGGATGCTGCCGGCGCTGCAGACGCTGCGGCGG TCCCTCCGCGCCGTGAAGCTGCTCCCGCCCGGGGAGGTGTTTGCCATCGAGAGCCGTCGCGTGCTGCGTCGCGAC GCTTTTGTCCTTCGCGACGGTGACGGGGCTGGCGTTGGACGGCCGGCCCAGAGGGTCATGCTCAGGTATATCCGT GACGTGGAGGCGCGCTTTGGCGAGATTCGTTTCGGGACGAGCATGTTGACGGATCATAGCCCCGCCGAGTACGAG GATGCGCTGAATATGTTGCGGTTGGGGGTTATTGAGTAG

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