© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Ophio5|3400 |
| Gene name | |
| Location | scaffold_27:38537..39658 |
| Strand | + |
| Gene length (bp) | 1121 |
| Transcript length (bp) | 960 |
| Coding sequence length (bp) | 957 |
| Protein length (aa) | 319 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF04116 | FA_hydroxylase | Fatty acid hydroxylase | 4.2E-23 | 173 | 306 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|P38992|SUR2_YEAST | Sphingolipid C4-hydroxylase SUR2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SUR2 PE=1 SV=1 | 2 | 318 | 1.0E-53 |
| sp|O94298|SUR2_SCHPO | Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=sur2 PE=3 SV=1 | 21 | 306 | 2.0E-50 |
| sp|Q9AST3|SBH2_ARATH | Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana GN=SBH2 PE=1 SV=1 | 17 | 306 | 1.0E-40 |
| sp|Q8VYI1|SBH1_ARATH | Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana GN=SBH1 PE=1 SV=1 | 162 | 306 | 5.0E-29 |
| sp|Q5R574|MSMO1_PONAB | Methylsterol monooxygenase 1 OS=Pongo abelii GN=MSMO1 PE=2 SV=1 | 177 | 318 | 5.0E-13 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|P38992|SUR2_YEAST | Sphingolipid C4-hydroxylase SUR2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SUR2 PE=1 SV=1 | 2 | 318 | 1.0E-53 |
| sp|O94298|SUR2_SCHPO | Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=sur2 PE=3 SV=1 | 21 | 306 | 2.0E-50 |
| sp|Q9AST3|SBH2_ARATH | Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana GN=SBH2 PE=1 SV=1 | 17 | 306 | 1.0E-40 |
| sp|Q8VYI1|SBH1_ARATH | Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana GN=SBH1 PE=1 SV=1 | 162 | 306 | 5.0E-29 |
| sp|Q5R574|MSMO1_PONAB | Methylsterol monooxygenase 1 OS=Pongo abelii GN=MSMO1 PE=2 SV=1 | 177 | 318 | 5.0E-13 |
| sp|Q15800|MSMO1_HUMAN | Methylsterol monooxygenase 1 OS=Homo sapiens GN=MSMO1 PE=1 SV=1 | 177 | 318 | 5.0E-13 |
| sp|Q6UGB2|MSMO1_PIG | Methylsterol monooxygenase 1 OS=Sus scrofa GN=MSMO1 PE=2 SV=1 | 177 | 318 | 1.0E-12 |
| sp|Q5ZLL6|MSMO1_CHICK | Methylsterol monooxygenase 1 OS=Gallus gallus GN=MSMO1 PE=2 SV=1 | 177 | 319 | 1.0E-12 |
| sp|Q9CRA4|MSMO1_MOUSE | Methylsterol monooxygenase 1 OS=Mus musculus GN=Msmo1 PE=1 SV=1 | 177 | 306 | 2.0E-12 |
| sp|Q4R4Q4|MSMO1_MACFA | Methylsterol monooxygenase 1 OS=Macaca fascicularis GN=MSMO1 PE=2 SV=1 | 177 | 306 | 3.0E-12 |
| sp|O35532|MSMO1_RAT | Methylsterol monooxygenase 1 OS=Rattus norvegicus GN=Msmo1 PE=2 SV=1 | 177 | 306 | 4.0E-12 |
| sp|Q5PRC0|CH25H_DANRE | Cholesterol 25-hydroxylase-like protein OS=Danio rerio GN=ch25h PE=2 SV=1 | 166 | 307 | 5.0E-12 |
| sp|Q7ZW77|MSMO1_DANRE | Methylsterol monooxygenase 1 OS=Danio rerio GN=msmo1 PE=2 SV=1 | 177 | 312 | 2.0E-11 |
| sp|Q1LX59|C2512_DANRE | Cholesterol 25-hydroxylase-like protein 1, member 2 OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1 | 175 | 306 | 5.0E-11 |
| sp|Q9UUH4|MSMO_SCHPO | Methylsterol monooxygenase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=erg25 PE=3 SV=1 | 128 | 318 | 5.0E-11 |
| sp|Q55D54|MSMOB_DICDI | Putative methylsterol monooxygenase DDB_G0269788 OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1 | 177 | 318 | 3.0E-10 |
| sp|Q8L7W5|SMO11_ARATH | Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana GN=SMO1-1 PE=1 SV=1 | 170 | 306 | 3.0E-10 |
| sp|Q9ZW22|SMO21_ARATH | Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana GN=SMO2-1 PE=2 SV=2 | 186 | 306 | 9.0E-10 |
| sp|Q1EC69|SMO12_ARATH | Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana GN=SMO1-2 PE=2 SV=1 | 166 | 306 | 1.0E-09 |
| sp|Q8VWZ8|SMO22_ARATH | Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana GN=SMO2-2 PE=2 SV=1 | 186 | 306 | 2.0E-09 |
| sp|A8WGT1|C25L2_DANRE | Cholesterol 25-hydroxylase-like protein 2 OS=Danio rerio GN=ch25hl2 PE=2 SV=1 | 183 | 319 | 2.0E-09 |
| sp|P53045|MSMO_YEAST | Methylsterol monooxygenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERG25 PE=1 SV=1 | 177 | 319 | 2.0E-09 |
| sp|Q567X1|C25L1_DANRE | Cholesterol 25-hydroxylase-like protein 1, member 1 OS=Danio rerio GN=ch25hl1.1 PE=2 SV=2 | 174 | 306 | 4.0E-09 |
| sp|Q55D52|MSMOA_DICDI | Putative methylsterol monooxygenase DDB_G0270946 OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2 | 177 | 319 | 5.0E-09 |
| sp|Q4G1G8|CH25H_PIG | Cholesterol 25-hydroxylase OS=Sus scrofa GN=CH25H PE=2 SV=1 | 170 | 307 | 5.0E-09 |
| sp|Q4QQV7|CH25H_RAT | Cholesterol 25-hydroxylase OS=Rattus norvegicus GN=Ch25h PE=2 SV=1 | 173 | 307 | 7.0E-09 |
| sp|Q9Z0F5|CH25H_MOUSE | Cholesterol 25-hydroxylase OS=Mus musculus GN=Ch25h PE=1 SV=1 | 173 | 307 | 1.0E-08 |
| sp|F4JLZ6|SMO13_ARATH | Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana GN=SMO1-3 PE=2 SV=1 | 183 | 306 | 3.0E-08 |
| sp|O59933|MSMO_CANAL | Methylsterol monooxygenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ERG25 PE=3 SV=1 | 177 | 319 | 4.0E-08 |
| sp|O95992|CH25H_HUMAN | Cholesterol 25-hydroxylase OS=Homo sapiens GN=CH25H PE=1 SV=1 | 183 | 314 | 3.0E-07 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0005506 | iron ion binding | Yes |
| GO:0016491 | oxidoreductase activity | Yes |
| GO:0008610 | lipid biosynthetic process | Yes |
| GO:0008152 | metabolic process | No |
| GO:0005488 | binding | No |
| GO:0009058 | biosynthetic process | No |
| GO:0071704 | organic substance metabolic process | No |
| GO:0046872 | metal ion binding | No |
| GO:0046914 | transition metal ion binding | No |
| GO:0044238 | primary metabolic process | No |
| GO:0006629 | lipid metabolic process | No |
| GO:0003824 | catalytic activity | No |
| GO:0003674 | molecular_function | No |
| GO:0043169 | cation binding | No |
| GO:0043167 | ion binding | No |
| GO:0008150 | biological_process | No |
| GO:1901576 | organic substance biosynthetic process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 31 | 0.5 |
| Domain # | Start | End | Length |
|---|---|---|---|
| 1 | 69 | 91 | 22 |
| 2 | 163 | 185 | 22 |
| 3 | 222 | 241 | 19 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| SC16a | Pure fungal culture | 0.07 | 0.00 | 0.38 |
| CcL | In ants, during behavior modification | 265.60 | 67.88 | 463.32 |
| CcD | In ants, recently dead | 217.81 | 109.43 | 326.19 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| SC16a | CcL | 0.295748 | no |
| SC16a | CcD | 0.295748 | no |
| CcL | CcD | 0.542530 | no |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Ophio5|3400 MAEQLVLRPKPPLIPWISDYNLSLIVPVVTHWTTAALYGLIERFELFQEYRIHTRDEERVKNRISRRKCLRGVIL VQLLQTGFGIALGMFSGVEMTGNEDHEVMKWASRVHNFGRTLTCYFPYDGSKVDMAQTTLQGLVDSTFLTSSLVE FQADLFVAKVIFYALMPACRLYLALWLADAWVFFIHRAEHRNAWLYRNFHARHHELYVPYSWGGIYDHPFESLFL SVGAFAIAVLGTGMTLRESILFSAFSSAKACTDHGGYKFPWNPMDLFTSVDAEYHDKHHQRRGLKTNFALHFQFW DRMFGTVFKGNEAAAKKQD |
| Coding | >Ophio5|3400 ATGGCTGAACAACTTGTCCTCCGTCCAAAACCACCGCTTATTCCTTGGATATCCGACTATAATCTTTCCTTGATT GTTCCCGTAGTGACTCATTGGACTACCGCGGCCCTCTACGGACTTATTGAGCGCTTTGAACTTTTCCAAGAATAT CGGATTCATACGCGCGATGAGGAAAGAGTTAAAAATAGGATCAGCCGACGAAAGTGCCTTCGCGGTGTCATCTTA GTCCAGCTCTTGCAGACCGGCTTTGGCATCGCTTTGGGCATGTTCTCAGGCGTAGAGATGACAGGCAATGAGGAT CACGAGGTCATGAAATGGGCAAGTCGTGTGCACAATTTTGGGCGCACGCTGACTTGCTATTTTCCCTATGATGGC TCTAAAGTCGACATGGCTCAAACGACGCTTCAAGGCTTGGTCGATTCTACATTTCTCACTTCCTCTTTGGTGGAA TTTCAGGCAGACTTGTTCGTCGCCAAGGTCATCTTCTACGCACTGATGCCTGCGTGTCGATTATACCTCGCCTTG TGGCTTGCGGATGCGTGGGTATTCTTTATTCATCGCGCCGAGCACAGAAATGCATGGTTATATAGGAACTTCCAC GCGCGCCATCACGAGTTGTACGTCCCGTACAGCTGGGGAGGGATCTATGACCACCCCTTTGAGAGCTTGTTTCTC AGTGTCGGGGCCTTTGCAATCGCTGTTCTTGGCACCGGGATGACGTTGAGAGAAAGCATACTCTTTTCAGCCTTT TCCTCCGCCAAAGCTTGCACAGATCATGGCGGATACAAGTTTCCTTGGAATCCGATGGATCTATTCACATCAGTC GACGCTGAGTATCATGACAAACACCATCAACGAAGAGGTTTAAAGACCAACTTTGCTCTTCACTTTCAGTTCTGG GATCGAATGTTTGGAACTGTGTTCAAGGGGAACGAAGCTGCAGCGAAGAAACAGGAC |
| Transcript | >Ophio5|3400 ATGGCTGAACAACTTGTCCTCCGTCCAAAACCACCGCTTATTCCTTGGATATCCGACTATAATCTTTCCTTGATT GTTCCCGTAGTGACTCATTGGACTACCGCGGCCCTCTACGGACTTATTGAGCGCTTTGAACTTTTCCAAGAATAT CGGATTCATACGCGCGATGAGGAAAGAGTTAAAAATAGGATCAGCCGACGAAAGTGCCTTCGCGGTGTCATCTTA GTCCAGCTCTTGCAGACCGGCTTTGGCATCGCTTTGGGCATGTTCTCAGGCGTAGAGATGACAGGCAATGAGGAT CACGAGGTCATGAAATGGGCAAGTCGTGTGCACAATTTTGGGCGCACGCTGACTTGCTATTTTCCCTATGATGGC TCTAAAGTCGACATGGCTCAAACGACGCTTCAAGGCTTGGTCGATTCTACATTTCTCACTTCCTCTTTGGTGGAA TTTCAGGCAGACTTGTTCGTCGCCAAGGTCATCTTCTACGCACTGATGCCTGCGTGTCGATTATACCTCGCCTTG TGGCTTGCGGATGCGTGGGTATTCTTTATTCATCGCGCCGAGCACAGAAATGCATGGTTATATAGGAACTTCCAC GCGCGCCATCACGAGTTGTACGTCCCGTACAGCTGGGGAGGGATCTATGACCACCCCTTTGAGAGCTTGTTTCTC AGTGTCGGGGCCTTTGCAATCGCTGTTCTTGGCACCGGGATGACGTTGAGAGAAAGCATACTCTTTTCAGCCTTT TCCTCCGCCAAAGCTTGCACAGATCATGGCGGATACAAGTTTCCTTGGAATCCGATGGATCTATTCACATCAGTC GACGCTGAGTATCATGACAAACACCATCAACGAAGAGGTTTAAAGACCAACTTTGCTCTTCACTTTCAGTTCTGG GATCGAATGTTTGGAACTGTGTTCAAGGGGAACGAAGCTGCAGCGAAGAAACAGGACTAG |
| Gene | >Ophio5|3400 ATGGCTGAACAACTTGTCCTCCGTCCAAAACCACCGCTTATTCCTTGGATATCCGACTATAATCTTTCCTTGATT GTTCCCGTAGTGACTCATTGGACTACCGCGGCCCTCTACGGACTTATTGAGCGCTTTGAACTTTTCCAAGAATAT CGGATTCATACGCGCGATGAGGAAAGAGTTAAAAATAGGATCAGCCGACGAAAGTGCCTTCGCGGTGTCATCTTA GTCCAGGTGTGCAAAGCCTTGTTTTCTCGGCTGACAATATCTCTGATATCTGAAGTGCAGCTCTTGCAGACCGGC TTTGGCATCGCTTTGGGCATGTTCTCAGGCGTAGAGATGACAGGCAATGAGGATCACGAGGTCATGAAATGGGCA AGTCGTGTGCACAATTTTGGGCGCACGCTGACTTGCTATTTTCCCTATGATGGCTCTAAAGTCGACATGGCTCAA ACGACGCTTCAAGGCTTGGTCGATTCTACATTTCTCACTTCCTCTTTGGTGGAATTTCAGGCAGACTTGTTCGTC GCCAAGGTCATCTTCTACGCACTGATGCCTGCGTGTCGATTATACCTCGCCTTGTGGCTTGCGGATGCGTGGGTA TTCTTTATTCATCGCGCCGAGCACAGAAATGCATGGTTATATAGTAAGTGGAATGAAGCACAGCAAAGGGACCCC CCCTGACGCCATGACCTAGGGAACTTCCACGCGCGCCATCACGAGTTGTACGTCCCGTACAGCTGGGGAGGGATC TATGACCACCCCTTTGAGAGCTTGTTTCTCAGTGTCGGGGCCTTTGCAATCGCTGTTCTTGGCACCGGGATGACG TTGAGAGAAAGCATACTCTTTTCAGCCTTTTCCTCCGCCAAAGCTTGCACAGATCATGGCGGATACAAGTTTCCT TGGAATCCGATGGATCTATTCACATCAGTCGACGCTGAGTATCATGACAAACACCATCAACGAAGAGGTTTAAAG GTAATTCCACTTGTATATTTTTTTTTTCGACTACTTCGCCCTAACAGTGATAATAGACCAACTTTGCTCTTCACT TTCAGTTCTGGGATCGAATGTTTGGAACTGTGTTCAAGGGGAACGAAGCTGCAGCGAAGAAACAGGACTAG |