© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Ophio5|2675 |
| Gene name | |
| Location | scaffold_217:831..3117 |
| Strand | - |
| Gene length (bp) | 2286 |
| Transcript length (bp) | 1743 |
| Coding sequence length (bp) | 1740 |
| Protein length (aa) | 580 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF01699 | Na_Ca_ex | Sodium/calcium exchanger protein | 3.3E-09 | 168 | 276 |
| PF01699 | Na_Ca_ex | Sodium/calcium exchanger protein | 3.1E-16 | 425 | 568 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q99385|VCX1_YEAST | Vacuolar calcium ion transporter OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VCX1 PE=1 SV=1 | 425 | 576 | 6.0E-32 |
| sp|O59768|VCX1_SCHPO | Vacuolar calcium ion transporter OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vcx1 PE=3 SV=1 | 425 | 576 | 1.0E-26 |
| sp|Q75XW3|CAX_APHHA | Ca(2+)/H(+) antiporter OS=Aphanothece halophytica PE=1 SV=1 | 427 | 578 | 9.0E-26 |
| sp|Q945S5|CAX4_ARATH | Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana GN=CAX4 PE=1 SV=2 | 399 | 568 | 2.0E-21 |
| sp|Q6K1C4|CAX3_ORYSJ | Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica GN=CAX3 PE=2 SV=2 | 425 | 568 | 1.0E-20 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q99385|VCX1_YEAST | Vacuolar calcium ion transporter OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VCX1 PE=1 SV=1 | 425 | 576 | 6.0E-32 |
| sp|O59768|VCX1_SCHPO | Vacuolar calcium ion transporter OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vcx1 PE=3 SV=1 | 425 | 576 | 1.0E-26 |
| sp|Q75XW3|CAX_APHHA | Ca(2+)/H(+) antiporter OS=Aphanothece halophytica PE=1 SV=1 | 427 | 578 | 9.0E-26 |
| sp|Q945S5|CAX4_ARATH | Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana GN=CAX4 PE=1 SV=2 | 399 | 568 | 2.0E-21 |
| sp|Q6K1C4|CAX3_ORYSJ | Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica GN=CAX3 PE=2 SV=2 | 425 | 568 | 1.0E-20 |
| sp|Q5KQN0|CAX2_ORYSJ | Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica GN=CAX2 PE=2 SV=2 | 425 | 568 | 5.0E-20 |
| sp|Q769E5|CAX1A_ORYSJ | Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica GN=CAX1a PE=1 SV=1 | 425 | 569 | 5.0E-20 |
| sp|Q6YXZ1|CAX4_ORYSJ | Putative vacuolar cation/proton exchanger 4 OS=Oryza sativa subsp. japonica GN=Os02g0138900 PE=3 SV=1 | 425 | 573 | 1.0E-19 |
| sp|P74072|CAX_SYNY3 | Ca(2+)/H(+) antiporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1336 PE=1 SV=1 | 427 | 578 | 2.0E-19 |
| sp|Q39254|CAX2_ARATH | Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana GN=CAX2 PE=1 SV=2 | 425 | 568 | 9.0E-19 |
| sp|Q8L783|CAX5_ARATH | Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana GN=CAX5 PE=2 SV=1 | 425 | 568 | 2.0E-18 |
| sp|Q39253|CAX1_ARATH | Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana GN=CAX1 PE=1 SV=3 | 440 | 570 | 5.0E-18 |
| sp|Q93Z81|CAX3_ARATH | Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana GN=CAX3 PE=1 SV=1 | 440 | 568 | 8.0E-18 |
| sp|Q9LFZ8|CAX6_ARATH | Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana GN=CAX6 PE=3 SV=3 | 425 | 568 | 2.0E-16 |
| sp|O59768|VCX1_SCHPO | Vacuolar calcium ion transporter OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vcx1 PE=3 SV=1 | 96 | 259 | 8.0E-15 |
| sp|Q5TKG3|CAX1B_ORYSJ | Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica GN=CAX1b PE=2 SV=1 | 443 | 568 | 4.0E-14 |
| sp|O34840|CHAA_BACSU | Ca(2+)/H(+) antiporter ChaA OS=Bacillus subtilis (strain 168) GN=chaA PE=1 SV=1 | 424 | 573 | 4.0E-13 |
| sp|Q99385|VCX1_YEAST | Vacuolar calcium ion transporter OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VCX1 PE=1 SV=1 | 109 | 280 | 6.0E-12 |
| sp|Q39254|CAX2_ARATH | Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana GN=CAX2 PE=1 SV=2 | 103 | 295 | 6.0E-11 |
| sp|Q8L783|CAX5_ARATH | Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana GN=CAX5 PE=2 SV=1 | 103 | 280 | 8.0E-11 |
| sp|Q5KTQ9|CAX1C_ORYSJ | Vacuolar cation/proton exchanger 1c OS=Oryza sativa subsp. japonica GN=CAX1c PE=2 SV=1 | 425 | 578 | 8.0E-11 |
| sp|Q6YXZ1|CAX4_ORYSJ | Putative vacuolar cation/proton exchanger 4 OS=Oryza sativa subsp. japonica GN=Os02g0138900 PE=3 SV=1 | 132 | 284 | 1.0E-10 |
| sp|Q9LFZ8|CAX6_ARATH | Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana GN=CAX6 PE=3 SV=3 | 65 | 280 | 1.0E-10 |
| sp|P74072|CAX_SYNY3 | Ca(2+)/H(+) antiporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1336 PE=1 SV=1 | 112 | 305 | 3.0E-09 |
| sp|Q6K1C4|CAX3_ORYSJ | Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica GN=CAX3 PE=2 SV=2 | 103 | 280 | 3.0E-09 |
| sp|Q5TKG3|CAX1B_ORYSJ | Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica GN=CAX1b PE=2 SV=1 | 116 | 280 | 5.0E-09 |
| sp|Q5KQN0|CAX2_ORYSJ | Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica GN=CAX2 PE=2 SV=2 | 168 | 280 | 1.0E-08 |
| sp|Q75XW3|CAX_APHHA | Ca(2+)/H(+) antiporter OS=Aphanothece halophytica PE=1 SV=1 | 111 | 305 | 1.0E-08 |
| sp|Q9P7B3|YI14_SCHPO | Putative cation exchanger C521.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC521.04c PE=1 SV=1 | 427 | 574 | 6.0E-07 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0016021 | integral component of membrane | Yes |
| GO:0055085 | transmembrane transport | Yes |
| GO:0009987 | cellular process | No |
| GO:0051234 | establishment of localization | No |
| GO:0006810 | transport | No |
| GO:0110165 | cellular anatomical entity | No |
| GO:0031224 | intrinsic component of membrane | No |
| GO:0005575 | cellular_component | No |
| GO:0051179 | localization | No |
| GO:0008150 | biological_process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 11 | 0.45 |
| Domain # | Start | End | Length |
|---|---|---|---|
| 1 | 104 | 121 | 17 |
| 2 | 125 | 147 | 22 |
| 3 | 154 | 176 | 22 |
| 4 | 186 | 208 | 22 |
| 5 | 221 | 243 | 22 |
| 6 | 253 | 275 | 22 |
| 7 | 423 | 445 | 22 |
| 8 | 460 | 482 | 22 |
| 9 | 489 | 511 | 22 |
| 10 | 521 | 543 | 22 |
| 11 | 556 | 578 | 22 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| SC16a | Pure fungal culture | 0.09 | 0.00 | 0.27 |
| CcL | In ants, during behavior modification | 110.08 | 45.52 | 174.64 |
| CcD | In ants, recently dead | 2.10 | 0.33 | 3.86 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| SC16a | CcL | 0.249569 | no |
| SC16a | CcD | 0.249569 | no |
| CcL | CcD | 0.000286 | yes |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Ophio5|2675 MEEASSNNARRHVCNNDSAAARDDDDDEPSSAELPGFDSASQSRLCALKDSTLHAFASWLVFAGKLKRCYRPTSS SPSDDEASGELRDQGWAYGPVAGPLCDMIFSSWLNALLFFVPVGLASKMAGLGPVVVFATNAAAIVPLSALLTDA TELHCLSLTLSLSLSLSLISIAALAHDHVRIVQASILGSVLVNLLLILGSALVVGSAANGEPAYNAADAQLLACL LSVSVFTFLMPTAFNYSFSPGTRSSGLLSLNFSRMSSLLILAIYVLYLFHEIRSRCRHTNVRALVDLESNLSIAE QRRLPPALLPLNTGHTVDMSSLHVPENGDPRVLHPDRGRRLADSRDDFRPRADSWQPSMSSRKLSPSPSFWSLSR DSSVGGGDVRRGFSRSSLTMQILRDVHPDRDQPVENRLTGGSAAHEVVVAIVMLVLTSGLMSINAELLVSTIDDV TRQTNLSEPVIGLILLPIVGNIAEYVTVVAVAAREKLDLAIAVAVGSSIQIALCVTPLTVLAGWILHKDLALTFN TFEIATLVGAVLLVNLLILNETGSSLRTGSLKGVLMCACYLIIGLGAYFAPAIEA |
| Coding | >Ophio5|2675 ATGGAAGAAGCTTCTTCGAATAACGCCCGTCGCCATGTCTGCAACAACGACTCCGCGGCTGCTCGAGATGACGAT GACGATGAGCCCTCTTCTGCCGAGCTGCCCGGCTTCGACTCTGCCTCTCAGTCTAGACTCTGCGCCTTGAAAGAC AGCACTCTGCATGCCTTTGCATCATGGCTCGTCTTCGCCGGCAAGCTCAAGCGCTGCTACCGCCCGACATCGTCG TCACCTTCTGACGACGAGGCTTCTGGAGAGCTTCGCGACCAAGGTTGGGCATATGGTCCCGTGGCCGGCCCGTTA TGTGACATGATCTTCTCGTCATGGCTTAACGCGCTGCTCTTCTTCGTTCCCGTCGGCCTGGCCAGCAAGATGGCT GGCTTGGGCCCTGTGGTCGTCTTTGCGACTAATGCAGCTGCCATCGTCCCGCTCTCGGCACTCTTGACTGACGCT ACTGAGCTTCACTGCCTCTCGCTGACTCTCTCTCTCTCTCTCTCTCTCTCTCTCATCAGCATTGCCGCCCTGGCC CACGACCACGTTCGCATCGTTCAGGCCTCTATTCTCGGCTCCGTTCTCGTCAACCTGCTACTGATCCTCGGCTCT GCCCTCGTCGTCGGCAGCGCCGCCAACGGCGAGCCGGCGTATAACGCGGCTGACGCTCAGCTTCTCGCTTGTCTA CTTTCCGTCTCCGTTTTTACCTTCCTGATGCCGACTGCCTTCAACTACTCTTTCAGCCCAGGCACGCGCTCATCC GGCCTTCTCAGCCTCAACTTCAGCAGGATGTCGTCTCTACTTATCCTCGCCATCTACGTCCTCTACTTATTCCAC GAGATTCGTTCGCGCTGCCGTCACACCAATGTTCGTGCCCTCGTCGATCTTGAAAGTAATCTCTCCATAGCCGAG CAACGCCGATTGCCGCCTGCTTTACTCCCCCTGAACACGGGCCACACCGTGGATATGAGCTCGCTTCACGTGCCA GAAAACGGCGATCCCAGAGTCCTTCATCCTGACCGAGGACGTCGTCTCGCCGATTCCAGAGACGACTTCAGGCCC CGCGCAGACTCGTGGCAGCCGTCCATGTCGTCACGGAAGCTTTCGCCTTCGCCGTCTTTTTGGTCCTTGAGCAGA GACTCCTCTGTTGGCGGTGGCGACGTCAGGAGAGGCTTTTCGCGATCCAGCCTAACGATGCAGATTCTGAGGGAC GTCCACCCCGATCGAGACCAGCCAGTCGAGAACCGACTCACAGGCGGCAGCGCAGCCCATGAAGTCGTCGTGGCC ATCGTCATGCTCGTCCTCACCTCCGGACTCATGTCCATCAACGCCGAACTTCTCGTCAGCACCATCGACGACGTC ACACGCCAGACGAACCTCTCTGAGCCCGTCATCGGCCTCATCCTGCTCCCTATCGTGGGCAACATCGCCGAGTAC GTCACCGTAGTGGCCGTGGCTGCACGTGAGAAGCTCGATCTGGCTATTGCCGTAGCTGTCGGCTCGTCTATCCAG ATTGCCTTGTGTGTCACGCCTCTGACGGTTCTGGCTGGTTGGATCTTGCACAAAGACTTGGCCTTGACCTTCAAC ACGTTCGAGATTGCCACGCTTGTCGGTGCTGTTTTACTTGTCAACCTTCTCATCCTCAATGAGACGGGCAGCAGT CTCCGGACCGGCAGCCTCAAGGGAGTCCTGATGTGTGCCTGCTATCTCATCATCGGACTAGGAGCGTACTTTGCC CCAGCGATTGAGGCG |
| Transcript | >Ophio5|2675 ATGGAAGAAGCTTCTTCGAATAACGCCCGTCGCCATGTCTGCAACAACGACTCCGCGGCTGCTCGAGATGACGAT GACGATGAGCCCTCTTCTGCCGAGCTGCCCGGCTTCGACTCTGCCTCTCAGTCTAGACTCTGCGCCTTGAAAGAC AGCACTCTGCATGCCTTTGCATCATGGCTCGTCTTCGCCGGCAAGCTCAAGCGCTGCTACCGCCCGACATCGTCG TCACCTTCTGACGACGAGGCTTCTGGAGAGCTTCGCGACCAAGGTTGGGCATATGGTCCCGTGGCCGGCCCGTTA TGTGACATGATCTTCTCGTCATGGCTTAACGCGCTGCTCTTCTTCGTTCCCGTCGGCCTGGCCAGCAAGATGGCT GGCTTGGGCCCTGTGGTCGTCTTTGCGACTAATGCAGCTGCCATCGTCCCGCTCTCGGCACTCTTGACTGACGCT ACTGAGCTTCACTGCCTCTCGCTGACTCTCTCTCTCTCTCTCTCTCTCTCTCTCATCAGCATTGCCGCCCTGGCC CACGACCACGTTCGCATCGTTCAGGCCTCTATTCTCGGCTCCGTTCTCGTCAACCTGCTACTGATCCTCGGCTCT GCCCTCGTCGTCGGCAGCGCCGCCAACGGCGAGCCGGCGTATAACGCGGCTGACGCTCAGCTTCTCGCTTGTCTA CTTTCCGTCTCCGTTTTTACCTTCCTGATGCCGACTGCCTTCAACTACTCTTTCAGCCCAGGCACGCGCTCATCC GGCCTTCTCAGCCTCAACTTCAGCAGGATGTCGTCTCTACTTATCCTCGCCATCTACGTCCTCTACTTATTCCAC GAGATTCGTTCGCGCTGCCGTCACACCAATGTTCGTGCCCTCGTCGATCTTGAAAGTAATCTCTCCATAGCCGAG CAACGCCGATTGCCGCCTGCTTTACTCCCCCTGAACACGGGCCACACCGTGGATATGAGCTCGCTTCACGTGCCA GAAAACGGCGATCCCAGAGTCCTTCATCCTGACCGAGGACGTCGTCTCGCCGATTCCAGAGACGACTTCAGGCCC CGCGCAGACTCGTGGCAGCCGTCCATGTCGTCACGGAAGCTTTCGCCTTCGCCGTCTTTTTGGTCCTTGAGCAGA GACTCCTCTGTTGGCGGTGGCGACGTCAGGAGAGGCTTTTCGCGATCCAGCCTAACGATGCAGATTCTGAGGGAC GTCCACCCCGATCGAGACCAGCCAGTCGAGAACCGACTCACAGGCGGCAGCGCAGCCCATGAAGTCGTCGTGGCC ATCGTCATGCTCGTCCTCACCTCCGGACTCATGTCCATCAACGCCGAACTTCTCGTCAGCACCATCGACGACGTC ACACGCCAGACGAACCTCTCTGAGCCCGTCATCGGCCTCATCCTGCTCCCTATCGTGGGCAACATCGCCGAGTAC GTCACCGTAGTGGCCGTGGCTGCACGTGAGAAGCTCGATCTGGCTATTGCCGTAGCTGTCGGCTCGTCTATCCAG ATTGCCTTGTGTGTCACGCCTCTGACGGTTCTGGCTGGTTGGATCTTGCACAAAGACTTGGCCTTGACCTTCAAC ACGTTCGAGATTGCCACGCTTGTCGGTGCTGTTTTACTTGTCAACCTTCTCATCCTCAATGAGACGGGCAGCAGT CTCCGGACCGGCAGCCTCAAGGGAGTCCTGATGTGTGCCTGCTATCTCATCATCGGACTAGGAGCGTACTTTGCC CCAGCGATTGAGGCGTAG |
| Gene | >Ophio5|2675 ATGGAAGAAGCTTCTTCGAATAACGCCCGTCGCCATGTCTGCAACAACGACTCCGCGGCTGCTCGAGATGACGAT GACGATGAGCCCTCTTCTGCCGAGCTGCCCGGCTTCGACTCTGCCTCTCAGTCTAGACTCTGCGCCTTGAAAGGT GAGCCCCTTTCCTATCAGAGAAAAAGGGGGGGAGAGAAGCCTCAGCAAAACCGCCGACTTCCTTCGTTTGCTCAA CATCGTCACTCTCTCCCAGACAGCACTCTGCATGCCTTTGCATCATGGCTCGTCTTCGCCGGCAAGCTCAAGCGC TGCTACCGCCCGACATCGTCGTCACCTTCTGACGACGAGGCTTCTGGAGAGCTTCGCGACCAAGGTTGGGCATAT GGTCCCGTGGCCGGCCCGTTATGTGACATGATCTTCTCGTCATGGCTTAACGCGCTGCTCTTCTTCGTTCCCGTC GGCCTGGCCAGCAAGATGGCTGGCTTGGGCCCTGTGGTCGTCTTTGCGACTAATGCAGCTGCCATCGTCCCGCTC TCGGCACTCTTGACTGACGCTACTGAGCGTGTCGCTGCTCATGCTGGCGATACCGTCGGCGCGCTGCTTAACATT AGCTTCGGAAACCTGGTTGAGCTGATTCTGTTGTACGGGCTCATGCTCCTTACTTCCTCTGCTACTTCTCCATTT CCTGCTCTCCTTTTTCCTATCCTCCCCCCTCAGTTCACTGCCTCTCGCTGACTCTCTCTCTCTCTCTCTCTCTCT CTCTCATCAGCATGTAAGTCGTGCTGCCTAAGTATTCAGTTATCAACTATAAACCACTCTCCGCCCCTTTCACCG CTCATCGCCTCCAGTGCCGCCCTGGCCCACGACCACGTTCGCATCGTTCAGGCCTCTATTCTCGGCTCCGTTCTC GTCAACCTGCTACTGATCCTCGGCTCTGCCCTCGTCGTCGGCAGCGCCGCCAACGGCGAGCCGGCGTATAACGCG GCTGACGCTCAGCTTCTCGCTTGTCTACTTTCCGTCTCCGTTTTTACCTTCCTGATGCCGGTACGGAAATGGTCC TCTATTGCCTTGAGAGCTCAGCTGACTCGTTCATGGCTTCAGACTGCCTTCAACTACTCTTTCAGCCCAGGCACG CGCTCATCCGGCCTTCTCAGCCTCAACTTCAGCAGGATGTCGTCTCTACTTATCCTCGCCATCTACGTCCTCTAC TTATTCCACGAGATTCGTTCGCGCTGCCGTCACACCAATGTTCGTGCCCTCGTCGATCTTGAAAGTAATCTCTCC ATAGCCGAGCAACGCCGATTGCCGCCTGCTTTACTCCCCCTGAACAGTCTGTCACATCCTACGTCCACTGGCGGT CCGCAGCCAATGTCACCTCGTACGATCCGTTTTATTGATGAGCCACCTTCTTCAGCGGGCCACACCGTGGATATG AGCTCGCTTCACGTGCCAGAAAACGGCGATCCCAGAGTCCTTCATCCTGACCGAGGACGTCGTCTCGCCGATTCC AGAGACGACTTCAGGCCCCGCGCAGACTCGTGGCAGCCGTCCATGTCGTCACGGAAGCTTTCGCCTTCGCCGTCT TTTTGGTCCTTGAGCAGAGACTCCTCTGTTGGCGGTGGCGACGTCAGGAGAGGCTTTTCGCGATCCAGCCTAACG ATGCAGATTCTGAGGGACGTCCACCCCGATCGAGACCAGCCAGTCGAGAACCGACTCACAGGCGGCAGCGCAGCC CATGAAGTCGTCGTGGCCATCGTCATGCTCGTCCTCACCTCCGGACTCATGTCCATCAACGCCGAACTTCTCGTC AGCACCATCGACGACGTCACACGCCAGACGAACCTCTCTGAGCCCGTCATCGGCCTCATCCTGCTCCCTATCGTG GGCAACATCGCCGAGTACGTCACCGTAGTGGCCGTGGCTGCACGTGAGAAGCTCGATCTGGCTATTGCCGTAGCT GTCGGCTCGTCTATCCAGATTGCCTTGTGTGTCACGCCTCTGACGGTTCTGGCTGGTTGGATCTTGCACAAAGAC TTGGCCTTGACCTTCAACACGTTCGAGATTGCCACGCTTGTCGGTGCTGTTTTACTTGTCAACCTTCTCATCCTC AATGAGACGGGCAGCAGTCTCCGGACCGGCAGCCTCAAGGGAGTCCTGATGTGTGCCTGCTATCTCATCATCGGG TTAGTGCCAGTTTATCGTCTTCGACTTAGGCTCGTCGTCTTGCTAACGTCTCTTTGTTTTGTGTTTCCAACTAGA CTAGGAGCGTACTTTGCCCCAGCGATTGAGGCGTAG |