© 2022 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | Ophio5|1577 |
| Gene name | |
| Location | scaffold_157:15410..16887 |
| Strand | - |
| Gene length (bp) | 1477 |
| Transcript length (bp) | 1428 |
| Coding sequence length (bp) | 1425 |
| Protein length (aa) | 475 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF00487 | FA_desaturase | Fatty acid desaturase | 3.8E-26 | 133 | 420 |
| PF11960 | DUF3474 | Domain of unknown function (DUF3474) | 5.8E-08 | 70 | 107 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|P59668|FAD12_MORIS | Delta(12) fatty acid desaturase OS=Mortierella isabellina PE=2 SV=1 | 74 | 461 | 2.0E-104 |
| sp|Q9Y8H5|FAD12_MORAP | Delta(12) fatty acid desaturase OS=Mortierella alpina PE=1 SV=2 | 74 | 461 | 3.0E-104 |
| sp|Q84VT2|FAD12_PUNGR | Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2 | 74 | 461 | 3.0E-93 |
| sp|A3F5L3|DES2_SORBI | Fatty acid desaturase DES2 OS=Sorghum bicolor GN=DES2 PE=2 SV=1 | 74 | 451 | 2.0E-91 |
| sp|P48631|FD6E2_SOYBN | Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN=FAD2-2 PE=2 SV=1 | 71 | 461 | 2.0E-91 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|P59668|FAD12_MORIS | Delta(12) fatty acid desaturase OS=Mortierella isabellina PE=2 SV=1 | 74 | 461 | 2.0E-104 |
| sp|Q9Y8H5|FAD12_MORAP | Delta(12) fatty acid desaturase OS=Mortierella alpina PE=1 SV=2 | 74 | 461 | 3.0E-104 |
| sp|Q84VT2|FAD12_PUNGR | Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2 | 74 | 461 | 3.0E-93 |
| sp|A3F5L3|DES2_SORBI | Fatty acid desaturase DES2 OS=Sorghum bicolor GN=DES2 PE=2 SV=1 | 74 | 451 | 2.0E-91 |
| sp|P48631|FD6E2_SOYBN | Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN=FAD2-2 PE=2 SV=1 | 71 | 461 | 2.0E-91 |
| sp|P48630|FD6E1_SOYBN | Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 1 OS=Glycine max GN=FAD2-1 PE=2 SV=1 | 72 | 461 | 6.0E-91 |
| sp|Q84UB9|FAD12_TRIKI | Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1 | 74 | 461 | 7.0E-90 |
| sp|Q39287|FAD6E_BRAJU | Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea PE=2 SV=1 | 72 | 461 | 5.0E-89 |
| sp|P46313|FAD6E_ARATH | Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana GN=FAD2 PE=1 SV=1 | 74 | 461 | 2.0E-88 |
| sp|Q41131|FH12_RICCO | Phosphatidylcholine 12-monooxygenase OS=Ricinus communis GN=FAH12 PE=1 SV=1 | 72 | 461 | 2.0E-86 |
| sp|D2CPE1|FH12M_RICCO | Phosphatidylcholine 12-monooxygenase OS=Ricinus communis GN=FAH12 PE=2 SV=1 | 72 | 461 | 6.0E-85 |
| sp|Q8GZC3|FAD2_VERFO | Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1 | 74 | 461 | 1.0E-84 |
| sp|Q84UB8|FADX_PUNGR | Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Punica granatum GN=FADX PE=1 SV=1 | 74 | 461 | 1.0E-81 |
| sp|Q9SP62|FADX_IMPBA | Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Impatiens balsamina GN=FADX PE=1 SV=1 | 73 | 461 | 1.0E-80 |
| sp|Q6RS95|FAD21_DIMSI | Acyl-lipid (9+3)-(E)-desaturase OS=Dimorphotheca sinuata PE=1 SV=1 | 71 | 461 | 1.0E-79 |
| sp|Q84UC0|FADX_TRIKI | Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAC PE=2 SV=1 | 74 | 461 | 2.0E-78 |
| sp|Q8GZC2|FADX_VERFO | Bifunctional desaturase/conjugase FADX OS=Vernicia fordii PE=1 SV=1 | 72 | 461 | 2.0E-77 |
| sp|O81931|FAD12_CREAL | Delta(12) fatty acid dehydrogenase OS=Crepis alpina PE=1 SV=2 | 74 | 461 | 3.0E-77 |
| sp|Q9SP61|FADX_MOMCH | Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia GN=FADX PE=1 SV=1 | 74 | 461 | 2.0E-74 |
| sp|Q6RS96|FAD22_DIMSI | Delta(9) fatty acid conjugase-like enzyme OS=Dimorphotheca sinuata PE=2 SV=1 | 74 | 460 | 7.0E-67 |
| sp|P48626|FAD3E_TOBAC | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum GN=FAD3 PE=2 SV=1 | 73 | 462 | 2.0E-59 |
| sp|P48619|FAD3C_RICCO | Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis GN=FAD7A-1 PE=2 SV=1 | 43 | 427 | 1.0E-51 |
| sp|P32291|FAD3E_VIGRR | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Vigna radiata var. radiata GN=ARG1 PE=2 SV=1 | 65 | 429 | 2.0E-51 |
| sp|P48622|FAD3D_ARATH | Temperature-sensitive omega-3 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD8 PE=2 SV=1 | 1 | 427 | 2.0E-49 |
| sp|P48620|FAD3C_SESIN | Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum GN=FAD7 PE=2 SV=1 | 73 | 429 | 5.0E-49 |
| sp|P48618|FAD3C_BRANA | Omega-3 fatty acid desaturase, chloroplastic (Fragment) OS=Brassica napus GN=FAD7 PE=2 SV=1 | 32 | 427 | 5.0E-49 |
| sp|P46310|FAD3C_ARATH | Omega-3 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD7 PE=2 SV=1 | 42 | 449 | 7.0E-49 |
| sp|P48623|FAD3E_ARATH | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Arabidopsis thaliana GN=FAD3 PE=2 SV=1 | 74 | 427 | 1.0E-48 |
| sp|P48625|FAD3E_SOYBN | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max GN=FAD3 PE=2 SV=1 | 73 | 449 | 4.0E-48 |
| sp|P48624|FAD3E_BRANA | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Brassica napus GN=FAD3 PE=2 SV=1 | 74 | 427 | 1.0E-46 |
| sp|A3F5L2|DES1_SORBI | Probable fatty acid desaturase DES1 OS=Sorghum bicolor GN=DES1 PE=2 SV=1 | 49 | 427 | 9.0E-46 |
| sp|Q594P3|DES3_SORBI | Fatty acid desaturase DES3 OS=Sorghum bicolor GN=DES3 PE=1 SV=1 | 49 | 425 | 7.0E-44 |
| sp|P48621|FAD3C_SOYBN | Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max GN=FAD7 PE=2 SV=1 | 3 | 426 | 3.0E-43 |
| sp|G5EGA5|FAT2_CAEEL | Delta(12) fatty acid desaturase fat-2 OS=Caenorhabditis elegans GN=fat-2 PE=2 SV=1 | 77 | 414 | 2.0E-37 |
| sp|Q9NEQ0|FAT1_CAEEL | Omega-3 fatty acid desaturase fat-1 OS=Caenorhabditis elegans GN=fat-1 PE=2 SV=1 | 77 | 460 | 9.0E-37 |
| sp|Q54794|DESA_ARTPT | Delta(12)-fatty-acid desaturase OS=Arthrospira platensis GN=desA PE=1 SV=1 | 136 | 423 | 5.0E-17 |
| sp|P48628|FAD6C_SOYBN | Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max PE=2 SV=1 | 75 | 417 | 3.0E-14 |
| sp|P46312|FAD6C_ARATH | Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD6 PE=1 SV=2 | 53 | 212 | 4.0E-12 |
| sp|P48629|FAD6C_SPIOL | Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea GN=FAD6 PE=1 SV=1 | 73 | 218 | 1.0E-11 |
| sp|P48627|FAD6C_BRANA | Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus PE=2 SV=1 | 53 | 212 | 1.0E-11 |
| sp|P20388|DESA_SYNY3 | Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=desA PE=1 SV=1 | 75 | 415 | 2.0E-10 |
| sp|O34653|DES_BACSU | Fatty acid desaturase OS=Bacillus subtilis (strain 168) GN=des PE=2 SV=1 | 138 | 201 | 5.0E-06 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0016717 | oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water | Yes |
| GO:0006629 | lipid metabolic process | Yes |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | No |
| GO:0071704 | organic substance metabolic process | No |
| GO:0044238 | primary metabolic process | No |
| GO:0016491 | oxidoreductase activity | No |
| GO:0003824 | catalytic activity | No |
| GO:0003674 | molecular_function | No |
| GO:0008152 | metabolic process | No |
| GO:0008150 | biological_process | No |
| SignalP signal predicted | Location (based on Ymax) |
D score (significance: > 0.45) |
|---|---|---|
| No | 1 - 11 | 0.45 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| SC16a | Pure fungal culture | 298.66 | 140.94 | 456.39 |
| CcL | In ants, during behavior modification | 649.60 | 210.26 | 1088.93 |
| CcD | In ants, recently dead | 476.10 | 197.17 | 755.03 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| SC16a | CcL | 0.009968 | yes |
| SC16a | CcD | 0.097013 | no |
| CcL | CcD | 0.354359 | no |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >Ophio5|1577 MASSAVSPCSPVLQRTITAATVTDSESSAVVSPSDSPRPSLSPSSTSLSSLASAEADSKYTGLVDTYGNAFTPPD FTIKDIRDAIPKHCFERSALTGYSYILRDLACLATTFVVFHNLVTPEIISSTPLRAALWAVYSVLQGLFATGIWV IAHECGHGAFSPSRLVNDVTGWVLHSALLVPYFSWQISHSKHHKATGNMERDMVFVPRTREQQVSRFGTVVREMG ELAEDTPVFTLVMLIMQQLVGWPNYLMTNVTGHDYHERQREGRGKNKKNGFGGGVNHFDPRSPLYEHKDAKLIVL SDIGIGLAATALIMLGNRFGWYNMAVWYFLPYLWVNHWLVAITFLQHTDPSLPHYTSDEWNFVRGAAATIDREMG FVGRHLLHGIIETHVLHHYVSSIPFYHADEATEAIRPVMGKHYRSDTEGGALGFIRALWRSARMCQWVEPSAEAR GSAKGILFFRNANGLGTKPMAAKTE |
| Coding | >Ophio5|1577 ATGGCCTCGTCCGCCGTATCCCCCTGCAGCCCCGTTCTGCAGCGCACCATCACCGCGGCCACAGTGACCGATTCG GAGTCTTCGGCCGTCGTGTCGCCTTCAGACTCGCCCCGGCCCTCGCTCTCGCCCTCTTCGACGTCTCTCTCTTCG CTCGCCTCGGCTGAAGCCGACTCCAAGTACACTGGGCTCGTCGATACGTACGGAAACGCCTTTACGCCCCCGGAC TTCACCATCAAGGATATCCGCGATGCCATTCCCAAGCACTGCTTCGAGCGCTCCGCCCTGACGGGCTACTCGTAC ATTCTCCGCGACCTGGCCTGCCTCGCCACCACTTTCGTCGTCTTCCACAATCTCGTCACGCCCGAAATAATCTCA TCGACGCCCCTCCGAGCCGCTCTCTGGGCCGTCTACTCGGTTTTGCAGGGCCTCTTTGCCACTGGCATTTGGGTC ATTGCCCACGAGTGTGGTCACGGTGCCTTCTCCCCTTCGCGCCTCGTCAACGACGTGACGGGCTGGGTCCTGCAC TCGGCCCTGCTCGTTCCCTATTTTAGCTGGCAAATCTCCCACAGCAAGCACCACAAAGCCACGGGCAATATGGAA CGCGATATGGTCTTCGTACCTCGCACCAGGGAGCAGCAGGTCTCCCGCTTCGGCACTGTCGTCCGCGAGATGGGC GAGCTGGCCGAGGACACACCGGTCTTCACCCTCGTGATGCTCATAATGCAGCAGCTCGTCGGCTGGCCCAACTAC CTCATGACAAACGTTACCGGCCACGACTACCACGAGCGTCAGCGCGAGGGCCGCGGCAAGAACAAGAAGAACGGC TTCGGCGGTGGCGTCAACCACTTTGATCCTCGCAGCCCGCTGTACGAGCACAAGGACGCCAAGCTCATCGTCCTC AGCGACATCGGCATCGGCCTGGCCGCGACAGCGCTCATCATGCTCGGCAACCGCTTCGGCTGGTACAACATGGCC GTCTGGTACTTTCTGCCCTATCTCTGGGTCAACCACTGGCTGGTGGCCATCACCTTCCTCCAGCACACCGATCCG TCTCTGCCTCACTACACTTCCGACGAGTGGAACTTTGTCCGCGGCGCGGCTGCCACCATCGACCGCGAGATGGGC TTCGTCGGCCGCCACCTGCTGCACGGCATCATCGAGACGCACGTCCTGCACCACTACGTCAGCTCCATCCCCTTT TATCACGCCGACGAGGCTACCGAGGCCATCCGCCCCGTCATGGGTAAGCACTACCGCTCCGACACCGAGGGCGGC GCGCTGGGCTTCATTCGCGCTCTCTGGCGAAGCGCCCGGATGTGCCAGTGGGTCGAGCCCAGCGCTGAGGCCCGG GGATCGGCCAAGGGCATCCTTTTCTTCCGCAACGCCAACGGCCTGGGAACCAAGCCCATGGCGGCCAAGACGGAG |
| Transcript | >Ophio5|1577 ATGGCCTCGTCCGCCGTATCCCCCTGCAGCCCCGTTCTGCAGCGCACCATCACCGCGGCCACAGTGACCGATTCG GAGTCTTCGGCCGTCGTGTCGCCTTCAGACTCGCCCCGGCCCTCGCTCTCGCCCTCTTCGACGTCTCTCTCTTCG CTCGCCTCGGCTGAAGCCGACTCCAAGTACACTGGGCTCGTCGATACGTACGGAAACGCCTTTACGCCCCCGGAC TTCACCATCAAGGATATCCGCGATGCCATTCCCAAGCACTGCTTCGAGCGCTCCGCCCTGACGGGCTACTCGTAC ATTCTCCGCGACCTGGCCTGCCTCGCCACCACTTTCGTCGTCTTCCACAATCTCGTCACGCCCGAAATAATCTCA TCGACGCCCCTCCGAGCCGCTCTCTGGGCCGTCTACTCGGTTTTGCAGGGCCTCTTTGCCACTGGCATTTGGGTC ATTGCCCACGAGTGTGGTCACGGTGCCTTCTCCCCTTCGCGCCTCGTCAACGACGTGACGGGCTGGGTCCTGCAC TCGGCCCTGCTCGTTCCCTATTTTAGCTGGCAAATCTCCCACAGCAAGCACCACAAAGCCACGGGCAATATGGAA CGCGATATGGTCTTCGTACCTCGCACCAGGGAGCAGCAGGTCTCCCGCTTCGGCACTGTCGTCCGCGAGATGGGC GAGCTGGCCGAGGACACACCGGTCTTCACCCTCGTGATGCTCATAATGCAGCAGCTCGTCGGCTGGCCCAACTAC CTCATGACAAACGTTACCGGCCACGACTACCACGAGCGTCAGCGCGAGGGCCGCGGCAAGAACAAGAAGAACGGC TTCGGCGGTGGCGTCAACCACTTTGATCCTCGCAGCCCGCTGTACGAGCACAAGGACGCCAAGCTCATCGTCCTC AGCGACATCGGCATCGGCCTGGCCGCGACAGCGCTCATCATGCTCGGCAACCGCTTCGGCTGGTACAACATGGCC GTCTGGTACTTTCTGCCCTATCTCTGGGTCAACCACTGGCTGGTGGCCATCACCTTCCTCCAGCACACCGATCCG TCTCTGCCTCACTACACTTCCGACGAGTGGAACTTTGTCCGCGGCGCGGCTGCCACCATCGACCGCGAGATGGGC TTCGTCGGCCGCCACCTGCTGCACGGCATCATCGAGACGCACGTCCTGCACCACTACGTCAGCTCCATCCCCTTT TATCACGCCGACGAGGCTACCGAGGCCATCCGCCCCGTCATGGGTAAGCACTACCGCTCCGACACCGAGGGCGGC GCGCTGGGCTTCATTCGCGCTCTCTGGCGAAGCGCCCGGATGTGCCAGTGGGTCGAGCCCAGCGCTGAGGCCCGG GGATCGGCCAAGGGCATCCTTTTCTTCCGCAACGCCAACGGCCTGGGAACCAAGCCCATGGCGGCCAAGACGGAG TGA |
| Gene | >Ophio5|1577 ATGGCCTCGTCCGCCGTATCCCCCTGCAGCCCCGTTCTGCAGCGCACCATCACCGCGGCCACAGTGACCGATTCG GAGTCTTCGGCCGTCGTGTCGCCTTCAGACTCGCCCCGGCCCTCGCTCTCGCCCTCTTCGACGTCTCTCTCTTCG CTCGCCTCGGCTGAAGCCGACTCCAAGTACACTGGGCTCGTCGATACGTACGGAAACGCCTTTACGCCCCCGGAC TTCACCATCAAGGATATCCGCGATGCCATTCCCAAGCACTGCTTCGAGCGCTCCGCCCTGACGGGCTACTCGTAC ATTCTCCGCGACCTGGCCTGCCTCGCCACCACTTTCGTCGTCTTCCACAATCTCGTCACGCCCGAAATAATCTCA TCGACGCCCCTCCGAGCCGCTCTCTGGGCCGTCTACTCGGTTTTGCAGGGCCTCTTTGCCACTGGCATTTGGGTC ATTGCCCACGAGTGTGGTCACGGTGCCTTCTCCCCTTCGCGCCTCGTCAACGACGTGACGGGCTGGGTCCTGCAC TCGGCCCTGCTCGTTCCCTATTTTAGCTGGCAAATCTCCCACAGCAAGCACCACAAAGCCACGGGCAATATGGAA CGCGATATGGTCTTCGTACCTCGCACCAGGGAGCAGCAGGTCTCCCGCTTCGGCACTGTCGTCCGCGAGATGGGC GAGCTGGCCGAGGACACACCGGTCTTCACCCTCGTGATGCTCATAATGCAGCAGCTCGTCGGCTGGCCCAACTAC CTCATGACAAACGTTACCGGCCACGACTACCACGAGCGTCAGCGCGAGGGCCGCGGCAAGAACAAGAAGAACGGC TTCGGCGGTGGCGTCAACCACTTTGATCCTCGCAGCCCGCTGTACGAGCACAAGGACGCCAAGCTCATCGTCCTC AGCGACATCGGCATCGGCCTGGCCGCGACAGCGCTCATCATGCTCGGCAACCGCTTCGGCTGGTACAACATGGCC GTCTGGTACTTTCTGCCCTATCTCTGGGTCAACCACTGGCTGGGTTCGTTGCCCTTTTCTCCAGCCTGCATATGT CTGCTGACTCTTTTCAGTGGCCATCACCTTCCTCCAGCACACCGATCCGTCTCTGCCTCACTACACTTCCGACGA GTGGAACTTTGTCCGCGGCGCGGCTGCCACCATCGACCGCGAGATGGGCTTCGTCGGCCGCCACCTGCTGCACGG CATCATCGAGACGCACGTCCTGCACCACTACGTCAGCTCCATCCCCTTTTATCACGCCGACGAGGCTACCGAGGC CATCCGCCCCGTCATGGGTAAGCACTACCGCTCCGACACCGAGGGCGGCGCGCTGGGCTTCATTCGCGCTCTCTG GCGAAGCGCCCGGATGTGCCAGTGGGTCGAGCCCAGCGCTGAGGCCCGGGGATCGGCCAAGGGCATCCTTTTCTT CCGCAACGCCAACGGCCTGGGAACCAAGCCCATGGCGGCCAAGACGGAGTGA |