Fungal Genomics

at Utrecht University

General Properties

Protein IDOphcf2|00310
Gene name
Locationscaffold_01:1444640..1447433
Strand-
Gene length (bp)2793
Transcript length (bp)2580
Coding sequence length (bp)2580
Protein length (aa) 860

Overview

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PFAM Domains

PFAM Domain ID Short name Long name E-value Start End
PF04082 Fungal_trans Fungal specific transcription factor domain 8.7E-13 191 372
PF00172 Zn_clus Fungal Zn(2)-Cys(6) binuclear cluster domain 1.6E-04 25 57

GO

GO Term Description Terminal node
GO:0003677 DNA binding Yes
GO:0008270 zinc ion binding Yes
GO:0006351 transcription, DNA-templated Yes
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific Yes
GO:0006355 regulation of transcription, DNA-templated Yes
GO:0043169 cation binding No
GO:0097159 organic cyclic compound binding No
GO:0009059 macromolecule biosynthetic process No
GO:0018130 heterocycle biosynthetic process No
GO:0140110 transcription regulator activity No
GO:0043170 macromolecule metabolic process No
GO:0010556 regulation of macromolecule biosynthetic process No
GO:1901576 organic substance biosynthetic process No
GO:0009058 biosynthetic process No
GO:0044237 cellular metabolic process No
GO:0005488 binding No
GO:0050794 regulation of cellular process No
GO:0006139 nucleobase-containing compound metabolic process No
GO:0009987 cellular process No
GO:0031326 regulation of cellular biosynthetic process No
GO:0080090 regulation of primary metabolic process No
GO:0031323 regulation of cellular metabolic process No
GO:0046483 heterocycle metabolic process No
GO:0034654 nucleobase-containing compound biosynthetic process No
GO:0008152 metabolic process No
GO:1901363 heterocyclic compound binding No
GO:0003700 DNA-binding transcription factor activity No
GO:1901362 organic cyclic compound biosynthetic process No
GO:0050789 regulation of biological process No
GO:0019219 regulation of nucleobase-containing compound metabolic process No
GO:0003676 nucleic acid binding No
GO:0019222 regulation of metabolic process No
GO:0044271 cellular nitrogen compound biosynthetic process No
GO:0034641 cellular nitrogen compound metabolic process No
GO:0051171 regulation of nitrogen compound metabolic process No
GO:0009889 regulation of biosynthetic process No
GO:0008150 biological_process No
GO:0043167 ion binding No
GO:0010468 regulation of gene expression No
GO:0019438 aromatic compound biosynthetic process No
GO:0060255 regulation of macromolecule metabolic process No
GO:0044249 cellular biosynthetic process No
GO:0065007 biological regulation No
GO:0097659 nucleic acid-templated transcription No
GO:2001141 regulation of RNA biosynthetic process No
GO:0071704 organic substance metabolic process No
GO:0051252 regulation of RNA metabolic process No
GO:0090304 nucleic acid metabolic process No
GO:1903506 regulation of nucleic acid-templated transcription No
GO:0003674 molecular_function No
GO:0046872 metal ion binding No
GO:0016070 RNA metabolic process No
GO:0006807 nitrogen compound metabolic process No
GO:0044238 primary metabolic process No
GO:0032774 RNA biosynthetic process No
GO:0006725 cellular aromatic compound metabolic process No
GO:0046914 transition metal ion binding No
GO:1901360 organic cyclic compound metabolic process No

Deeploc

[Help with interpreting the results of Deeploc 2.0]
Localizations Signals Cytoplasm Nucleus Extracellular Cell membrane Mitochondrion Plastid Endoplasmic reticulum Lysosome vacuole Golgi apparatus Peroxisome
Nucleus Nuclear localization signal 0.3263 0.834 0.0585 0.0523 0.053 0.0042 0.067 0.0445 0.0291 0.0024

SignalP

(None)

Transmembrane Domains

(None)

Transcription Factor Class

Transcription Factor Class
(based on PFAM domains)
Fungal Specific TF

CAZymes

(None)

Secondary Metabolism

(None)

Expression data

Analysis 1: Expression analysis during behavioral modification. Published in: Will et al., G3, 2020

Click here for more information

Orthologs

Orthofinder run ID4
Orthogroup143
Change Orthofinder run
Species Protein ID
Ophiocordyceps australis 1348a (Ghana) OphauG2|2202
Ophiocordyceps australis 1348a (Ghana) OphauG2|6675
Ophiocordyceps australis map64 (Brazil) OphauB2|2050
Ophiocordyceps australis map64 (Brazil) OphauB2|4346
Ophiocordyceps camponoti-floridani Ophcf2|00310 (this protein)
Ophiocordyceps camponoti-floridani Ophcf2|05265
Ophiocordyceps camponoti-rufipedis Ophun1|5134
Ophiocordyceps camponoti-rufipedis Ophun1|550
Ophiocordyceps kimflemingae Ophio5|1223
Ophiocordyceps kimflemingae Ophio5|394
Ophiocordyceps subramaniannii Hirsu2|2919
Ophiocordyceps subramaniannii Hirsu2|4664

Sequences

Type of sequenceSequence
Locus Download genbank file of locus Download genbank file of locus (reverse complement)
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein >Ophcf2|00310
MSTAAVSAVATSTTVDSTSGYNRSACTECQRRKQKCNREWPCDRCQRRKIADECRYNYSNAGPVATTTTTTTTTT
TNSYSPSAPVAAAVSTVVGSHGSRLEEQPRTMPRAEIEPTSSSSSSDPDTPGFDALAAARLLTNLGIESKAWNVK
PSETASKQDLAAADATPQMKRLLELLPPRQTMDKLINDFTHNINYHYYIIYPPDFLRDYHIWWERRSKGKPMALQ
YTGLLAMICACCVQHAEDEMQSELRQASGLSAEDLSEQLHSAVRELASVMPVGRFHMFNVQRLLHSCYWYKAEAR
FVEAWHVLSAAIREGQELGCHREPAEGTETEHEREMRRRLWCVLDTWDWQISSGLSRPKIIDRDDCTAELPSLTL
ERYPGRTPSPLLHMKMQSELTRQLASRFSAPKNIQEPAQVLEYQSLIEAWMKRFPAVYDFEQPDLSCDGQYPWVF
PHRYYVYTMACLLILNPIRHYMVRTLTPASPPDELAIRATGVAYSLKLLRTLRSWVDAIVHRDGRMHFIVFSIFD
TAAILCTAVIKDGHETLAEREDMTLAIADALAMLRRLNTISSTSRTSYDLLERLVRRLPASTPFAKHQQQQPRKR
ARMVGLSSSSGGGSGSGSGSGSGSGSGVINHFDLAPVPHQQQHQAHHNHPLNNHHRNHHHHHHHHQYPHPQNRPP
PSSEATTLPSKASSPQHEHASAYQAPIRATTTPYRRPTPPQPPSPYPHPTTQQHQPTQTTHPHARNAPPRPRRPP
PLLALLPRTHRPRPRRRMPPHPHGQPLPPGPTFGFPDESDLGLPPAPFELETVTQAQLGELAPLWNWHSENLQIV
HCASSSASAHPDLLLPSTAVPVAAFPMSSPMPPP*
Coding >Ophcf2|00310
ATGAGCACCGCCGCCGTGAGCGCCGTCGCCACATCCACGACGGTGGACTCTACCTCGGGATACAACCGCTCGGCA
TGTACCGAGTGCCAGCGTCGCAAGCAAAAGTGCAACCGCGAGTGGCCTTGTGACCGGTGCCAGCGTCGCAAGATT
GCCGATGAATGCCGCTACAACTACTCGAATGCCGGTCCCGTGGCGACGACGACGACGACGACGACTACGACGACG
ACCAATAGCTACTCGCCTTCGGCCCCCGTCGCCGCCGCGGTGAGCACCGTCGTCGGCAGCCATGGGAGCCGCCTC
GAGGAGCAACCGCGGACGATGCCCCGGGCTGAGATTGAACCGACGTCGTCGTCGTCGTCGTCGGATCCCGACACG
CCTGGCTTCGACGCCCTAGCCGCTGCCCGACTTCTCACCAACCTGGGCATCGAGTCCAAGGCGTGGAACGTCAAA
CCGTCCGAGACAGCGTCCAAGCAAGATCTGGCTGCCGCCGACGCGACGCCGCAGATGAAGCGTTTGCTGGAGCTT
CTCCCACCACGGCAGACGATGGACAAGCTCATCAACGACTTCACGCACAATATCAATTATCACTATTACATCATA
TACCCGCCCGACTTCTTGCGCGACTACCACATCTGGTGGGAGAGGCGGTCCAAGGGTAAGCCGATGGCGCTGCAG
TACACTGGCCTGCTGGCCATGATCTGCGCCTGCTGCGTGCAGCACGCCGAGGACGAGATGCAGAGCGAGCTGCGG
CAGGCTTCGGGCTTGAGCGCCGAGGACCTATCGGAGCAGTTGCACAGCGCGGTGCGGGAGCTGGCGAGCGTCATG
CCCGTGGGCCGCTTCCACATGTTCAACGTGCAGCGGCTGCTGCATTCGTGTTACTGGTACAAGGCCGAGGCCCGC
TTCGTCGAGGCCTGGCACGTCCTGAGCGCGGCCATTCGGGAGGGTCAGGAGCTGGGCTGTCACCGAGAGCCGGCA
GAGGGGACGGAGACGGAGCACGAGCGCGAGATGCGGCGGAGGCTGTGGTGCGTGCTGGACACGTGGGACTGGCAA
ATCTCGTCGGGCCTCTCTCGGCCCAAGATCATCGACCGCGACGACTGTACGGCCGAGCTCCCCTCGCTGACGCTG
GAGCGGTATCCCGGTCGAACGCCGTCACCGCTGCTGCACATGAAGATGCAGTCGGAGCTGACTCGACAGCTGGCT
TCGAGATTCAGCGCGCCCAAGAACATACAGGAGCCGGCTCAGGTGCTCGAGTACCAGTCGCTGATCGAGGCTTGG
ATGAAGCGCTTCCCGGCCGTCTACGACTTTGAACAGCCGGATCTCTCTTGCGACGGTCAGTATCCGTGGGTGTTT
CCGCACCGCTACTACGTCTACACCATGGCCTGCCTCCTCATCCTCAACCCGATCCGCCACTACATGGTGCGGACG
CTGACGCCGGCCTCGCCTCCGGACGAGCTGGCCATCCGGGCGACGGGCGTGGCCTATTCCCTCAAGCTGCTGCGC
ACGCTGAGGAGCTGGGTCGATGCCATCGTCCATCGCGACGGTCGTATGCACTTTATCGTCTTCTCCATCTTCGAC
ACGGCCGCCATCTTGTGCACGGCCGTCATCAAGGATGGACACGAGACGTTGGCCGAGAGGGAAGACATGACGCTG
GCCATTGCCGATGCTCTGGCCATGCTTCGTCGTCTCAATACCATCTCTAGCACGTCGAGGACGTCGTACGACCTG
CTCGAGCGTCTTGTCCGTCGTCTTCCCGCCTCGACGCCTTTTGCCAAGCATCAGCAGCAGCAGCCGCGCAAGAGG
GCCAGGATGGTTGGTCTATCGTCGTCATCCGGCGGAGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGC
TCGGGCGTCATCAACCACTTTGATCTCGCGCCCGTGCCTCATCAGCAACAGCATCAAGCTCATCACAACCACCCC
CTTAACAACCACCACCGCAACCACCACCACCACCACCACCACCATCAGTACCCTCACCCCCAAAACCGTCCCCCC
CCGTCCTCAGAAGCAACCACGCTCCCCTCCAAGGCCTCATCCCCCCAACACGAACATGCCTCAGCATACCAGGCC
CCGATCCGTGCCACCACAACCCCCTATCGTCGACCAACACCGCCCCAACCCCCTTCCCCATACCCCCATCCAACA
ACACAACAGCACCAACCTACGCAGACCACCCATCCTCATGCTCGGAATGCACCTCCCCGGCCTCGACGACCGCCT
CCTCTCCTCGCTCTCCTCCCCCGAACGCACAGGCCTCGTCCTCGTCGACGGATGCCACCCCATCCCCATGGCCAA
CCCCTCCCCCCCGGCCCAACCTTTGGCTTCCCAGACGAATCCGACCTCGGCCTCCCCCCGGCGCCCTTTGAGCTC
GAGACGGTGACGCAGGCTCAGCTGGGAGAGCTAGCCCCCCTCTGGAACTGGCACTCTGAGAATCTCCAAATCGTC
CACTGCGCCTCTTCCTCGGCATCGGCCCATCCCGATCTCCTCCTCCCTTCTACGGCCGTCCCCGTGGCAGCGTTC
CCGATGTCGTCTCCCATGCCGCCGCCTTGA
Transcript >Ophcf2|00310
ATGAGCACCGCCGCCGTGAGCGCCGTCGCCACATCCACGACGGTGGACTCTACCTCGGGATACAACCGCTCGGCA
TGTACCGAGTGCCAGCGTCGCAAGCAAAAGTGCAACCGCGAGTGGCCTTGTGACCGGTGCCAGCGTCGCAAGATT
GCCGATGAATGCCGCTACAACTACTCGAATGCCGGTCCCGTGGCGACGACGACGACGACGACGACTACGACGACG
ACCAATAGCTACTCGCCTTCGGCCCCCGTCGCCGCCGCGGTGAGCACCGTCGTCGGCAGCCATGGGAGCCGCCTC
GAGGAGCAACCGCGGACGATGCCCCGGGCTGAGATTGAACCGACGTCGTCGTCGTCGTCGTCGGATCCCGACACG
CCTGGCTTCGACGCCCTAGCCGCTGCCCGACTTCTCACCAACCTGGGCATCGAGTCCAAGGCGTGGAACGTCAAA
CCGTCCGAGACAGCGTCCAAGCAAGATCTGGCTGCCGCCGACGCGACGCCGCAGATGAAGCGTTTGCTGGAGCTT
CTCCCACCACGGCAGACGATGGACAAGCTCATCAACGACTTCACGCACAATATCAATTATCACTATTACATCATA
TACCCGCCCGACTTCTTGCGCGACTACCACATCTGGTGGGAGAGGCGGTCCAAGGGTAAGCCGATGGCGCTGCAG
TACACTGGCCTGCTGGCCATGATCTGCGCCTGCTGCGTGCAGCACGCCGAGGACGAGATGCAGAGCGAGCTGCGG
CAGGCTTCGGGCTTGAGCGCCGAGGACCTATCGGAGCAGTTGCACAGCGCGGTGCGGGAGCTGGCGAGCGTCATG
CCCGTGGGCCGCTTCCACATGTTCAACGTGCAGCGGCTGCTGCATTCGTGTTACTGGTACAAGGCCGAGGCCCGC
TTCGTCGAGGCCTGGCACGTCCTGAGCGCGGCCATTCGGGAGGGTCAGGAGCTGGGCTGTCACCGAGAGCCGGCA
GAGGGGACGGAGACGGAGCACGAGCGCGAGATGCGGCGGAGGCTGTGGTGCGTGCTGGACACGTGGGACTGGCAA
ATCTCGTCGGGCCTCTCTCGGCCCAAGATCATCGACCGCGACGACTGTACGGCCGAGCTCCCCTCGCTGACGCTG
GAGCGGTATCCCGGTCGAACGCCGTCACCGCTGCTGCACATGAAGATGCAGTCGGAGCTGACTCGACAGCTGGCT
TCGAGATTCAGCGCGCCCAAGAACATACAGGAGCCGGCTCAGGTGCTCGAGTACCAGTCGCTGATCGAGGCTTGG
ATGAAGCGCTTCCCGGCCGTCTACGACTTTGAACAGCCGGATCTCTCTTGCGACGGTCAGTATCCGTGGGTGTTT
CCGCACCGCTACTACGTCTACACCATGGCCTGCCTCCTCATCCTCAACCCGATCCGCCACTACATGGTGCGGACG
CTGACGCCGGCCTCGCCTCCGGACGAGCTGGCCATCCGGGCGACGGGCGTGGCCTATTCCCTCAAGCTGCTGCGC
ACGCTGAGGAGCTGGGTCGATGCCATCGTCCATCGCGACGGTCGTATGCACTTTATCGTCTTCTCCATCTTCGAC
ACGGCCGCCATCTTGTGCACGGCCGTCATCAAGGATGGACACGAGACGTTGGCCGAGAGGGAAGACATGACGCTG
GCCATTGCCGATGCTCTGGCCATGCTTCGTCGTCTCAATACCATCTCTAGCACGTCGAGGACGTCGTACGACCTG
CTCGAGCGTCTTGTCCGTCGTCTTCCCGCCTCGACGCCTTTTGCCAAGCATCAGCAGCAGCAGCCGCGCAAGAGG
GCCAGGATGGTTGGTCTATCGTCGTCATCCGGCGGAGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGC
TCGGGCGTCATCAACCACTTTGATCTCGCGCCCGTGCCTCATCAGCAACAGCATCAAGCTCATCACAACCACCCC
CTTAACAACCACCACCGCAACCACCACCACCACCACCACCACCATCAGTACCCTCACCCCCAAAACCGTCCCCCC
CCGTCCTCAGAAGCAACCACGCTCCCCTCCAAGGCCTCATCCCCCCAACACGAACATGCCTCAGCATACCAGGCC
CCGATCCGTGCCACCACAACCCCCTATCGTCGACCAACACCGCCCCAACCCCCTTCCCCATACCCCCATCCAACA
ACACAACAGCACCAACCTACGCAGACCACCCATCCTCATGCTCGGAATGCACCTCCCCGGCCTCGACGACCGCCT
CCTCTCCTCGCTCTCCTCCCCCGAACGCACAGGCCTCGTCCTCGTCGACGGATGCCACCCCATCCCCATGGCCAA
CCCCTCCCCCCCGGCCCAACCTTTGGCTTCCCAGACGAATCCGACCTCGGCCTCCCCCCGGCGCCCTTTGAGCTC
GAGACGGTGACGCAGGCTCAGCTGGGAGAGCTAGCCCCCCTCTGGAACTGGCACTCTGAGAATCTCCAAATCGTC
CACTGCGCCTCTTCCTCGGCATCGGCCCATCCCGATCTCCTCCTCCCTTCTACGGCCGTCCCCGTGGCAGCGTTC
CCGATGTCGTCTCCCATGCCGCCGCCTTGA
Gene >Ophcf2|00310
ATGAGCACCGCCGCCGTGAGCGCCGTCGCCACATCCACGACGGTGGACTCTACCTCGGGATACAACCGCTCGGCA
TGTACCGAGTGCCAGCGTCGCAAGCAAAAGGCTCGTAGCCAGGGGAGAAGCATCTGTCGCCAACTCTTGCTGACG
ACGGTCTAGTGCAACCGCGAGTGGCCTTGTGACCGGTGCCAGCGTCGCAAGATTGCCGATGAATGCCGCTACAAC
TACTCGAATGCCGGTCCCGTGGCGACGACGACGACGACGACGACTACGACGACGACCAATAGCTACTCGCCTTCG
GCCCCCGTCGCCGCCGCGGTGAGCACCGTCGTCGGCAGCCATGGGAGCCGCCTCGAGGAGCAACCGCGGACGATG
CCCCGGGCTGAGATTGAACCGACGTCGTCGTCGTCGTCGTCGGATCCCGACACGCCTGGCTTCGACGCCCTAGCC
GCTGCCCGACTTCTCACCAACCTGGGCATCGAGTCCAAGGCGTGGAACGTCAAACCGTCCGAGACAGCGTCCAAG
CAAGATCTGGCTGCCGCCGACGCGACGCCGCAGATGAAGCGTTTGCTGGAGCTTCTCCCACCACGGCAGACGATG
GGTGAGCTATTCGACTCCCTACGTTGACTCACAAACAAATAAAAGAAGCTGACAGTTGGGTAGACAAGCTCATCA
ACGACTTCACGCACAATATCAATTATCACTATTACATCATATACCCGCCCGACTTCTTGCGCGACTACCACATCT
GGTGGGAGAGGCGGTCCAAGGGTAAGCCGATGGCGCTGCAGTACACTGGCCTGCTGGCCATGATCTGCGCCTGCT
GCGTGCAGCACGCCGAGGACGAGATGCAGAGCGAGCTGCGGCAGGCTTCGGGCTTGAGCGCCGAGGACCTATCGG
AGCAGTTGCACAGCGCGGTGCGGGAGCTGGCGAGCGTCATGCCCGTGGGCCGCTTCCACATGTTCAACGTGCAGC
GGCTGCTGCATTCGTGTTACTGGTACAAGGCCGAGGCCCGCTTCGTCGAGGCCTGGCACGTCCTGAGCGCGGCCA
TTCGGGAGGGTCAGGAGCTGGGCTGTCACCGAGAGCCGGCAGAGGGGACGGAGACGGAGCACGAGCGCGAGATGC
GGCGGAGGCTGTGGTGCGTGCTGGACACGTGGGACTGGTGAGAGTGAGAGAGAGATTACCGAGATGTGGTCCCTC
CTGTCCCTGGTTCCTCAGAGTTGCTGACCACACACACACACACTCTCTCTCTACATCAGGCAAATCTCGTCGGGC
CTCTCTCGGCCCAAGATCATCGACCGCGACGACTGTACGGCCGAGCTCCCCTCGCTGACGCTGGAGCGGTATCCC
GGTCGAACGCCGTCACCGCTGCTGCACATGAAGATGCAGTCGGAGCTGACTCGACAGCTGGCTTCGAGATTCAGC
GCGCCCAAGAACATACAGGAGCCGGCTCAGGTGCTCGAGTACCAGTCGCTGATCGAGGCTTGGATGAAGCGCTTC
CCGGCCGTCTACGACTTTGAACAGCCGGATCTCTCTTGCGACGGTCAGTATCCGTGGGTGTTTCCGCACCGCTAC
TACGTCTACACCATGGCCTGCCTCCTCATCCTCAACCCGATCCGCCACTACATGGTGCGGACGCTGACGCCGGCC
TCGCCTCCGGACGAGCTGGCCATCCGGGCGACGGGCGTGGCCTATTCCCTCAAGCTGCTGCGCACGCTGAGGAGC
TGGGTCGATGCCATCGTCCATCGCGACGGTCGTATGCACTTTATCGTCTTCTCCATCTTCGACACGGCCGCCATC
TTGTGCACGGCCGTCATCAAGGATGGACACGAGACGTTGGCCGAGAGGGAAGACATGACGCTGGCCATTGCCGAT
GCTCTGGCCATGCTTCGTCGTCTCAATACCATCTCTAGCACGTCGAGGACGTCGTACGACCTGCTCGAGCGTCTT
GTCCGTCGTCTTCCCGCCTCGACGCCTTTTGCCAAGCATCAGCAGCAGCAGCCGCGCAAGAGGGCCAGGATGGTT
GGTCTATCGTCGTCATCCGGCGGAGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCGTCATC
AACCACTTTGATCTCGCGCCCGTGCCTCATCAGCAACAGCATCAAGCTCATCACAACCACCCCCTTAACAACCAC
CACCGCAACCACCACCACCACCACCACCACCATCAGTACCCTCACCCCCAAAACCGTCCCCCCCCGTCCTCAGAA
GCAACCACGCTCCCCTCCAAGGCCTCATCCCCCCAACACGAACATGCCTCAGCATACCAGGCCCCGATCCGTGCC
ACCACAACCCCCTATCGTCGACCAACACCGCCCCAACCCCCTTCCCCATACCCCCATCCAACAACACAACAGCAC
CAACCTACGCAGACCACCCATCCTCATGCTCGGAATGCACCTCCCCGGCCTCGACGACCGCCTCCTCTCCTCGCT
CTCCTCCCCCGAACGCACAGGCCTCGTCCTCGTCGACGGATGCCACCCCATCCCCATGGCCAACCCCTCCCCCCC
GGCCCAACCTTTGGCTTCCCAGACGAATCCGACCTCGGCCTCCCCCCGGCGCCCTTTGAGCTCGAGACGGTGACG
CAGGCTCAGCTGGGAGAGCTAGCCCCCCTCTGGAACTGGCACTCTGAGAATCTCCAAATCGTCCACTGCGCCTCT
TCCTCGGCATCGGCCCATCCCGATCTCCTCCTCCCTTCTACGGCCGTCCCCGTGGCAGCGTTCCCGATGTCGTCT
CCCATGCCGCCGCCTTGA

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