Fungal Genomics

Agaricus bisporus var bisporus H39 Agaricus bisporus var bisporus H97 Agaricus bisporus var burnettii H119p4 Aspergillus niger CBS 112.32 Aspergillus niger CBS 113.50 Aspergillus niger CBS 115988 Aspergillus niger CBS 115989 Aspergillus niger CBS 118.52 Aspergillus niger CBS 124.48 Aspergillus niger CBS 131.52 Aspergillus niger CBS 133816 Aspergillus niger CBS 147320 Aspergillus niger CBS 147321 Aspergillus niger CBS 147322 Aspergillus niger CBS 147323 Aspergillus niger CBS 147324 Aspergillus niger CBS 147343 Aspergillus niger CBS 147344 Aspergillus niger CBS 147345 Aspergillus niger CBS 147346 Aspergillus niger CBS 147347 Aspergillus niger CBS 147352 Aspergillus niger CBS 147353 Aspergillus niger CBS 147371 Aspergillus niger CBS 147482 Aspergillus niger CBS 630.78 Aspergillus niger CBS 769.97 Niveomyces coronatus CBS 149186 Ophiocordyceps australis 1348a (Ghana) Ophiocordyceps australis map64 (Brazil) Ophiocordyceps camponoti-floridani Ophiocordyceps camponoti-rufipedis Ophiocordyceps kimflemingae Ophiocordyceps subramaniannii Penicillium roqueforti DTO002I6 Penicillium roqueforti DTO003C3 Penicillium roqueforti DTO003H1 (CBS 147308) Penicillium roqueforti DTO006G1 Penicillium roqueforti DTO006G7 Penicillium roqueforti DTO012A1 Penicillium roqueforti DTO012A2 (CBS 147309) Penicillium roqueforti DTO012A6 (CBS 147310) Penicillium roqueforti DTO012A7 Penicillium roqueforti DTO012A8 Penicillium roqueforti DTO012A9 Penicillium roqueforti DTO013E5 Penicillium roqueforti DTO013F2 Penicillium roqueforti DTO013F5 (CBS 147311) Penicillium roqueforti DTO027I6 Penicillium roqueforti DTO032C6 Penicillium roqueforti DTO039G3 Penicillium roqueforti DTO046C5 Penicillium roqueforti DTO070G2 (CBS 147317) Penicillium roqueforti DTO081F9 (CBS 147318) Penicillium roqueforti DTO101D6 (CBS 147325) Penicillium roqueforti DTO102I9 (CBS 147326) Penicillium roqueforti DTO126G2 (CBS 147330) Penicillium roqueforti DTO127F7 (CBS 147331) Penicillium roqueforti DTO127F9 (CBS 147332) Penicillium roqueforti DTO130C1 (CBS 147333) Penicillium roqueforti DTO163C3 (CBS 147337) Penicillium roqueforti DTO163F5 (CBS 147338) Penicillium roqueforti DTO163G4 (CBS 147339) Penicillium roqueforti DTO265D5 (CBS 147372) Penicillium roqueforti DTO369A1 (CBS 147354) Penicillium roqueforti DTO375B1 (CBS 147355) Penicillium roqueforti DTO377G2 (LCP 96.3914a) Penicillium roqueforti LCP96 04111 Rhodonia placenta FPRL280 Torrubiellomyces zombiae CBS 149187

General Properties

Protein ID	AgabiH97|104580
Gene name
Location	scaffold_7:1875331..1878392
Strand	+
Gene length (bp)	3061
Transcript length (bp)	1854
Coding sequence length (bp)	1854
Protein length (aa)	618

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PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF09423	PhoD	PhoD-like phosphatase	3.3E-98	209	570
PF16655	PhoD_N	PhoD-like phosphatase, N-terminal domain	1.3E-26	90	190

Swissprot hits

Swissprot ID	Swissprot Description	Start	End	E-value
sp|P42251|PPBD_BACSU	Alkaline phosphatase D OS=Bacillus subtilis (strain 168) GN=phoD PE=1 SV=3	84	573	7.0E-69
sp|Q8KRU5|PLD_STRCW	Phospholipase D OS=Streptomyces chromofuscus GN=pld PE=1 SV=2	88	600	1.0E-56

GO

(None)

SignalP

[Help with interpreting these statistics]

SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
Yes	1 - 17	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Developmental stages of Agaricus bisporus (strain A15). Published in Pelkmans et al, Applied Microbiology and Biotechnology, 2016

Expression values

Label	Description	Expression (RPKM)	Confidence interval (low)	Confidence interval (high)
Casing	Casing mycelium	143.69	71.34	216.04
Initials	Initials knots	19.13	10.18	28.07
Pileal_Stipeal_center	Stage I stipe center	43.13	25.71	60.55
Pileal_Stipeal_shell	Stage I stipe shell	147.89	85.56	210.22
DIF_stipe_center	Stage II stipe center	35.14	19.52	50.76
DIF_stipe_shell	Stage II stipe shell	160.20	83.12	237.28
DIF_stipe_skin	Stage II stipe skin	111.29	63.10	159.49
DIF_cap_skin	Stage II cap skin	294.61	153.45	435.78
DIF_cap_tissue	Stage II cap tissue	130.31	64.23	196.40
DIF_gill_tissue	Stage II gill tissue	46.40	26.39	66.41
YFB_stipe_center	Young fruiting body stipe center	162.78	82.00	243.56
YFB_stipe_shell	Young fruiting body stipe shell	352.66	168.30	537.02
YFB_stipe_skin	Young fruiting body stipe skin	334.17	173.33	495.00
YFB_cap_skin	Young fruiting body cap skin	645.59	288.55	1002.63
YFB_cap_tissue	Young fruiting body cap tissue	146.24	84.56	207.93
YFB_gill_tissue	Young fruiting body gill tissue	513.86	207.69	820.04
YFB_veil	Young fruiting body veil	248.77	125.76	371.78

Differential expression

Label1	Label2	Q-value	Significant difference
Casing	DIF_gill_tissue	0.000613	yes
Casing	YFB_stipe_center	0.829461	no
Casing	YFB_stipe_shell	0.009446	yes
Casing	YFB_stipe_skin	0.008457	yes
Casing	YFB_cap_skin	0.000613	yes
Casing	YFB_cap_tissue	0.977008	no
Casing	YFB_gill_tissue	0.000613	yes
Casing	YFB_veil	0.128525	no
Casing	Initials	0.000613	yes
Casing	Pileal_Stipeal_center	0.000613	yes
Casing	Pileal_Stipeal_shell	0.963168	no
Casing	DIF_stipe_center	0.000613	yes
Casing	DIF_stipe_shell	0.855870	no
Casing	DIF_stipe_skin	0.551402	no
Casing	DIF_cap_skin	0.030372	yes
Casing	DIF_cap_tissue	0.879194	no
DIF_gill_tissue	YFB_stipe_center	0.000613	yes
DIF_gill_tissue	YFB_stipe_shell	0.000613	yes
DIF_gill_tissue	YFB_stipe_skin	0.000613	yes
DIF_gill_tissue	YFB_cap_skin	0.000613	yes
DIF_gill_tissue	YFB_cap_tissue	0.000613	yes
DIF_gill_tissue	YFB_gill_tissue	0.000613	yes
DIF_gill_tissue	YFB_veil	0.000613	yes
YFB_stipe_center	YFB_stipe_shell	0.018896	yes
YFB_stipe_center	YFB_stipe_skin	0.016386	yes
YFB_stipe_center	YFB_cap_skin	0.000613	yes
YFB_stipe_center	YFB_cap_tissue	0.835784	no
YFB_stipe_center	YFB_gill_tissue	0.000613	yes
YFB_stipe_center	YFB_veil	0.244275	no
YFB_stipe_shell	YFB_stipe_skin	0.933019	no
YFB_stipe_shell	YFB_cap_skin	0.105537	no
YFB_stipe_shell	YFB_cap_tissue	0.004928	yes
YFB_stipe_shell	YFB_gill_tissue	0.408913	no
YFB_stipe_shell	YFB_veil	0.398297	no
YFB_stipe_skin	YFB_cap_skin	0.053965	no
YFB_stipe_skin	YFB_cap_tissue	0.002084	yes
YFB_stipe_skin	YFB_gill_tissue	0.291145	no
YFB_stipe_skin	YFB_veil	0.467739	no
YFB_cap_skin	YFB_cap_tissue	0.000613	yes
YFB_cap_skin	YFB_gill_tissue	0.678001	no
YFB_cap_skin	YFB_veil	0.002951	yes
YFB_cap_tissue	YFB_gill_tissue	0.000613	yes
YFB_cap_tissue	YFB_veil	0.092675	no
YFB_gill_tissue	YFB_veil	0.042909	yes
Initials	DIF_gill_tissue	0.001625	yes
Initials	YFB_stipe_center	0.000613	yes
Initials	YFB_stipe_shell	0.000613	yes
Initials	YFB_stipe_skin	0.000613	yes
Initials	YFB_cap_skin	0.000613	yes
Initials	YFB_cap_tissue	0.000613	yes
Initials	YFB_gill_tissue	0.000613	yes
Initials	YFB_veil	0.000613	yes
Initials	Pileal_Stipeal_center	0.003365	yes
Initials	Pileal_Stipeal_shell	0.000613	yes
Initials	DIF_stipe_center	0.049882	yes
Initials	DIF_stipe_shell	0.000613	yes
Initials	DIF_stipe_skin	0.000613	yes
Initials	DIF_cap_skin	0.000613	yes
Initials	DIF_cap_tissue	0.000613	yes
Pileal_Stipeal_center	DIF_gill_tissue	0.887593	no
Pileal_Stipeal_center	YFB_stipe_center	0.000613	yes
Pileal_Stipeal_center	YFB_stipe_shell	0.000613	yes
Pileal_Stipeal_center	YFB_stipe_skin	0.000613	yes
Pileal_Stipeal_center	YFB_cap_skin	0.000613	yes
Pileal_Stipeal_center	YFB_cap_tissue	0.000613	yes
Pileal_Stipeal_center	YFB_gill_tissue	0.000613	yes
Pileal_Stipeal_center	YFB_veil	0.000613	yes
Pileal_Stipeal_center	Pileal_Stipeal_shell	0.000613	yes
Pileal_Stipeal_center	DIF_stipe_center	0.610576	no
Pileal_Stipeal_center	DIF_stipe_shell	0.000613	yes
Pileal_Stipeal_center	DIF_stipe_skin	0.000613	yes
Pileal_Stipeal_center	DIF_cap_skin	0.000613	yes
Pileal_Stipeal_center	DIF_cap_tissue	0.000613	yes
Pileal_Stipeal_shell	DIF_gill_tissue	0.000613	yes
Pileal_Stipeal_shell	YFB_stipe_center	0.856231	no
Pileal_Stipeal_shell	YFB_stipe_shell	0.009446	yes
Pileal_Stipeal_shell	YFB_stipe_skin	0.004160	yes
Pileal_Stipeal_shell	YFB_cap_skin	0.000613	yes
Pileal_Stipeal_shell	YFB_cap_tissue	0.983681	no
Pileal_Stipeal_shell	YFB_gill_tissue	0.000613	yes
Pileal_Stipeal_shell	YFB_veil	0.103472	no
Pileal_Stipeal_shell	DIF_stipe_center	0.000613	yes
Pileal_Stipeal_shell	DIF_stipe_shell	0.886479	no
Pileal_Stipeal_shell	DIF_stipe_skin	0.413497	no
Pileal_Stipeal_shell	DIF_cap_skin	0.019987	yes
Pileal_Stipeal_shell	DIF_cap_tissue	0.809086	no
DIF_stipe_center	DIF_gill_tissue	0.460023	no
DIF_stipe_center	YFB_stipe_center	0.000613	yes
DIF_stipe_center	YFB_stipe_shell	0.000613	yes
DIF_stipe_center	YFB_stipe_skin	0.000613	yes
DIF_stipe_center	YFB_cap_skin	0.000613	yes
DIF_stipe_center	YFB_cap_tissue	0.000613	yes
DIF_stipe_center	YFB_gill_tissue	0.000613	yes
DIF_stipe_center	YFB_veil	0.000613	yes
DIF_stipe_center	DIF_stipe_shell	0.000613	yes
DIF_stipe_center	DIF_stipe_skin	0.000613	yes
DIF_stipe_center	DIF_cap_skin	0.000613	yes
DIF_stipe_center	DIF_cap_tissue	0.000613	yes
DIF_stipe_shell	DIF_gill_tissue	0.000613	yes
DIF_stipe_shell	YFB_stipe_center	0.980819	no
DIF_stipe_shell	YFB_stipe_shell	0.016669	yes
DIF_stipe_shell	YFB_stipe_skin	0.016386	yes
DIF_stipe_shell	YFB_cap_skin	0.000613	yes
DIF_stipe_shell	YFB_cap_tissue	0.864650	no
DIF_stipe_shell	YFB_gill_tissue	0.000613	yes
DIF_stipe_shell	YFB_veil	0.214244	no
DIF_stipe_shell	DIF_stipe_skin	0.297484	no
DIF_stipe_shell	DIF_cap_skin	0.052948	no
DIF_stipe_shell	DIF_cap_tissue	0.670778	no
DIF_stipe_skin	DIF_gill_tissue	0.001140	yes
DIF_stipe_skin	YFB_stipe_center	0.281732	no
DIF_stipe_skin	YFB_stipe_shell	0.000613	yes
DIF_stipe_skin	YFB_stipe_skin	0.000613	yes
DIF_stipe_skin	YFB_cap_skin	0.000613	yes
DIF_stipe_skin	YFB_cap_tissue	0.447765	no
DIF_stipe_skin	YFB_gill_tissue	0.000613	yes
DIF_stipe_skin	YFB_veil	0.006032	yes
DIF_stipe_skin	DIF_cap_skin	0.000613	yes
DIF_stipe_skin	DIF_cap_tissue	0.754100	no
DIF_cap_skin	DIF_gill_tissue	0.000613	yes
DIF_cap_skin	YFB_stipe_center	0.074840	no
DIF_cap_skin	YFB_stipe_shell	0.728471	no
DIF_cap_skin	YFB_stipe_skin	0.812776	no
DIF_cap_skin	YFB_cap_skin	0.017507	yes
DIF_cap_skin	YFB_cap_tissue	0.018624	yes
DIF_cap_skin	YFB_gill_tissue	0.145255	no
DIF_cap_skin	YFB_veil	0.734158	no
DIF_cap_skin	DIF_cap_tissue	0.010412	yes
DIF_cap_tissue	DIF_gill_tissue	0.000613	yes
DIF_cap_tissue	YFB_stipe_center	0.642998	no
DIF_cap_tissue	YFB_stipe_shell	0.002525	yes
DIF_cap_tissue	YFB_stipe_skin	0.003765	yes
DIF_cap_tissue	YFB_cap_skin	0.000613	yes
DIF_cap_tissue	YFB_cap_tissue	0.826161	no
DIF_cap_tissue	YFB_gill_tissue	0.000613	yes
DIF_cap_tissue	YFB_veil	0.053965	no

Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>AgabiH97|104580 MRFLLAALSLAAGTYAIVDKLDKNLAFRSPFLNEPQFGHDIAAISRRSAQSQRRDTVDASPFKDEPYPGFYSSDF DNAPTVWSSGINFTHSVASGDSLDTSIILWTRAVPLPASNGELPIASIPVCVSYKIATDENLGQSVDTGEAFTTY DVDFTVKVEAKNLKPDTKYWYQFADCTNEKTVSPIGMTRTLSSADTPAEEVNNGNPLTFAVFSCSNFAAGYFNAY GFAAHNVTPDVFIHLGDYMYEDFGNGGSIGRLVLGRELATIADYRHRFEQYRSDLDLQFAHQTFPWITVWVSTSL DDHEIANNGWKAGTSDSNDTASGCMFSPSGACFTDRKMAGTRAYHEWMPIRQVDAKDQLRIWRNFQIGKLLDLTM LDTRHYNRDITDLGYNAALVESLSNDVERSIMGHSQENWLSETLSQSKSREAIWRVIGQQVVFTQLLQNTRLDFD AWDGYRSNRNRILDQLYGKNIDNTVILSGDSHANWVSDLAHINDTETYDPLTGSGAIGVEFAGTGVTSSSGTFKP NGPLEFSNNVSMSLVQENIDLQWSEGHFRGFFTIRVTPEFLNATYYSMQNATFRNLDGFASANFSVKAGANRLTR PVANGNILSGALKPGNH*
Coding	>AgabiH97|104580 ATGCGTTTCTTACTTGCGGCTTTATCCCTTGCCGCCGGTACATATGCCATCGTTGACAAGCTTGATAAAAATTTG GCTTTCCGTTCTCCCTTTCTGAATGAACCCCAGTTCGGTCACGATATAGCTGCAATAAGCAGAAGAAGTGCACAA TCTCAACGAAGGGATACTGTAGACGCTTCACCTTTCAAAGATGAACCTTATCCCGGATTTTATTCCTCTGACTTC GATAATGCCCCGACCGTCTGGAGCTCAGGAATCAATTTCACTCATTCCGTGGCGAGCGGCGATTCCCTTGACACT TCCATCATTCTTTGGACGCGAGCTGTTCCGCTTCCCGCAAGCAATGGTGAACTACCGATCGCAAGTATTCCGGTC TGCGTTTCCTACAAGATCGCTACAGACGAAAATCTTGGTCAATCCGTCGACACCGGGGAAGCTTTTACAACTTAT GACGTAGATTTCACGGTCAAGGTAGAAGCTAAGAATTTGAAGCCAGACACCAAATACTGGTATCAATTTGCAGAC TGCACAAACGAGAAGACTGTCAGCCCGATTGGAATGACTCGTACTCTGTCCTCGGCTGACACGCCCGCGGAGGAA GTCAACAACGGAAACCCTTTGACATTTGCAGTTTTCTCCTGCTCCAATTTCGCAGCCGGATATTTCAATGCTTAC GGATTCGCGGCACATAACGTTACCCCCGACGTTTTCATTCATTTGGGAGACTACATGTATGAGGATTTTGGTAAC GGTGGCTCTATCGGGCGCCTTGTTCTTGGTCGGGAATTAGCGACTATTGCCGATTATCGTCACCGCTTCGAACAG TATAGGAGTGATCTTGATCTGCAATTTGCGCATCAGACATTTCCATGGATAACAGTTTGGGTAAGCACTAGTTTA GACGACCATGAGATCGCAAACAATGGTTGGAAAGCGGGAACATCGGATTCAAACGATACAGCATCGGGATGTATG TTTTCACCCTCAGGTGCCTGTTTCACAGACCGAAAAATGGCTGGTACTCGTGCATATCATGAGTGGATGCCCATA CGACAAGTCGACGCCAAGGACCAGCTTCGCATTTGGAGAAATTTCCAGATTGGAAAATTATTGGACTTGACCATG CTTGATACTCGTCACTACAACCGTGATATTACTGATCTTGGATACAACGCTGCTCTCGTTGAATCTCTCAGCAAC GACGTCGAGCGTTCCATAATGGGACACAGCCAAGAAAACTGGCTCTCCGAAACGCTTTCCCAATCAAAATCCCGC GAAGCAATTTGGCGTGTTATCGGCCAACAAGTGGTATTCACACAGCTGCTGCAGAATACACGACTTGACTTCGAT GCTTGGGATGGCTACCGCTCGAACAGAAATCGCATTCTTGACCAATTATACGGCAAAAATATTGACAATACGGTC ATCTTATCAGGTGATAGTCATGCCAATTGGGTATCGGACCTCGCTCACATCAATGACACGGAGACATATGATCCA CTGACAGGCAGTGGCGCAATCGGCGTCGAGTTTGCTGGGACCGGTGTCACATCTTCCTCCGGGACATTCAAACCA AATGGTCCTCTTGAATTTTCCAACAACGTCTCAATGTCCCTCGTACAAGAGAACATAGACTTGCAATGGAGTGAA GGGCATTTCAGGGGATTCTTTACGATCCGGGTTACACCCGAATTTTTGAATGCCACTTACTATTCAATGCAGAAC GCTACTTTCCGGAATTTAGATGGTTTCGCATCTGCCAATTTTAGCGTTAAAGCTGGGGCTAACCGTTTGACGCGC CCAGTTGCCAATGGTAATATTCTTAGTGGTGCACTGAAACCTGGAAACCACTAG
Transcript	>AgabiH97|104580 ATGCGTTTCTTACTTGCGGCTTTATCCCTTGCCGCCGGTACATATGCCATCGTTGACAAGCTTGATAAAAATTTG GCTTTCCGTTCTCCCTTTCTGAATGAACCCCAGTTCGGTCACGATATAGCTGCAATAAGCAGAAGAAGTGCACAA TCTCAACGAAGGGATACTGTAGACGCTTCACCTTTCAAAGATGAACCTTATCCCGGATTTTATTCCTCTGACTTC GATAATGCCCCGACCGTCTGGAGCTCAGGAATCAATTTCACTCATTCCGTGGCGAGCGGCGATTCCCTTGACACT TCCATCATTCTTTGGACGCGAGCTGTTCCGCTTCCCGCAAGCAATGGTGAACTACCGATCGCAAGTATTCCGGTC TGCGTTTCCTACAAGATCGCTACAGACGAAAATCTTGGTCAATCCGTCGACACCGGGGAAGCTTTTACAACTTAT GACGTAGATTTCACGGTCAAGGTAGAAGCTAAGAATTTGAAGCCAGACACCAAATACTGGTATCAATTTGCAGAC TGCACAAACGAGAAGACTGTCAGCCCGATTGGAATGACTCGTACTCTGTCCTCGGCTGACACGCCCGCGGAGGAA GTCAACAACGGAAACCCTTTGACATTTGCAGTTTTCTCCTGCTCCAATTTCGCAGCCGGATATTTCAATGCTTAC GGATTCGCGGCACATAACGTTACCCCCGACGTTTTCATTCATTTGGGAGACTACATGTATGAGGATTTTGGTAAC GGTGGCTCTATCGGGCGCCTTGTTCTTGGTCGGGAATTAGCGACTATTGCCGATTATCGTCACCGCTTCGAACAG TATAGGAGTGATCTTGATCTGCAATTTGCGCATCAGACATTTCCATGGATAACAGTTTGGGTAAGCACTAGTTTA GACGACCATGAGATCGCAAACAATGGTTGGAAAGCGGGAACATCGGATTCAAACGATACAGCATCGGGATGTATG TTTTCACCCTCAGGTGCCTGTTTCACAGACCGAAAAATGGCTGGTACTCGTGCATATCATGAGTGGATGCCCATA CGACAAGTCGACGCCAAGGACCAGCTTCGCATTTGGAGAAATTTCCAGATTGGAAAATTATTGGACTTGACCATG CTTGATACTCGTCACTACAACCGTGATATTACTGATCTTGGATACAACGCTGCTCTCGTTGAATCTCTCAGCAAC GACGTCGAGCGTTCCATAATGGGACACAGCCAAGAAAACTGGCTCTCCGAAACGCTTTCCCAATCAAAATCCCGC GAAGCAATTTGGCGTGTTATCGGCCAACAAGTGGTATTCACACAGCTGCTGCAGAATACACGACTTGACTTCGAT GCTTGGGATGGCTACCGCTCGAACAGAAATCGCATTCTTGACCAATTATACGGCAAAAATATTGACAATACGGTC ATCTTATCAGGTGATAGTCATGCCAATTGGGTATCGGACCTCGCTCACATCAATGACACGGAGACATATGATCCA CTGACAGGCAGTGGCGCAATCGGCGTCGAGTTTGCTGGGACCGGTGTCACATCTTCCTCCGGGACATTCAAACCA AATGGTCCTCTTGAATTTTCCAACAACGTCTCAATGTCCCTCGTACAAGAGAACATAGACTTGCAATGGAGTGAA GGGCATTTCAGGGGATTCTTTACGATCCGGGTTACACCCGAATTTTTGAATGCCACTTACTATTCAATGCAGAAC GCTACTTTCCGGAATTTAGATGGTTTCGCATCTGCCAATTTTAGCGTTAAAGCTGGGGCTAACCGTTTGACGCGC CCAGTTGCCAATGGTAATATTCTTAGTGGTGCACTGAAACCTGGAAACCACTAG
Gene	>AgabiH97|104580 ATGCGTTTCTTACTTGCGGCTTTATCCCTTGCCGCCGGTACATATGCCATCGTTGACAAGCTTGATAAAAATTTG GCTTTCCGTTCTCCCTTTCTGAATGAACCCCAGGTGAGCCACTGCCAAAATTGCTGTTAGAGAGCGGCTCTTATA TTTCAACAGTTCGGTCACGATATAGCTGCAATAAGCAGAAGAAGTGCACAATCTCAACGAAGGGATACTGTAGAC GCTTCACCTTTCAAAGATGAACCTTATCCCGGATTTTATTCCTCTGACTTCGATAATGTTAGATCTGTTGCTGTT GAATGTTGCTGTACTATTCTGAGCTATGATAAACCTAGGCCCCGACCGTCTGGAGCTCAGGAATCAATTTCACTC ATTCCGGTACGTTTTCAAGGAGTAAAAAACCGTATACGAATTTAATTATGATACATAGTGGCGAGCGTAAGTCTC GAGTTGCGAGGCATCAGGTTAGAAGTTTGATTCGTCGAAACCAGGGCGATTCCCTTGACACTTCCATCATTCTTT GGACGCGAGCTGTTCCGCTTCCCGCAAGCAATGGTGAACTACCGATCGCAAGTATTCCGGTCTGCGTTTCCTACA AGATCGCTACAGACGAAAATCTTGGTCAATCCGTCGACACCGGGGAAGCTTTTACAACTTATGACGTAGATTTCA CGGTCAAGGTAGAAGCTAAGAATTTGAAGCCAGACACCAAATACTGGTATCAATTTGCAGACTGCACAAACGAGA AGACTGTCAGCCCGATTGGAATGACTCGTACTCTGTCCTCGGCTGACAGTGAGTCATTTTCTGCTTATGGGTGAT ACCAACACTGACCTTGTTTCTTGCACTCCAGCGCCCGCGGAGGAAGTCAACAACGGAAACCCTTTGACATTTGCA GTTTTCTCCTGCTCCAATTTCGCAGCCGGTAAATTCATTTCCCCATAGCCCTGCTAAAGCCTAATGAGACCAATT AGGATATTTCAATGCTTACGGATTCGCGGCACATAACGTTACCCCCGACGTTTTCATTCATTTGGGAGACTACGT AAATGACATTTCAAGATTCAATCTCCTCTCAGGATATTAATGCTTATTTGGGTCCTTAGATGTATGAGGATTTTG GTAACGGGTAAGTCAAGGATAGAAATTTCGAAATGCTGAACCTAACGCGCCAATTGATCGAACATAGTGGCTCTA TCGGGCGCCTTGTTCTTGGTCGGGAATTAGCGACTATTGCCGATTATCGTCACCGCTTCGAACAGTATAGGAGTG ATCTTGATCTGCAATTTGCGCATCAGACATTTCCATGGATAACAGTTTGGTGAGTTTCTTTTTTCAACTATATTA GGAGATATGATAACTTACACCAATCGCGTTAATGCAAGGGTAAGCACTAGTTTAGTTCCCTTTGTAGGTACTTCC AAACGAATTACCTCCGGCCACTTTTTAGGACGACCATGAGGTTAGCAGAATGCCAGATCAAATAATCAGCGGACT CATTCTATAATGCCTTAGATCGCAAACAATGGTTGGAAAGCGGGAACATCGGATTCAAACGATACAGCATCGGGA TGTATGTTTTCACCCTCAGGTGCCTGTTTCACAGACCGAAAAATGGCTGGTACTCGTGCATATCATGAGTGGATG CCCATACGACAAGTCGACGCCAAGGACCAGCTTCGCATTTGGAGAAATTTCCAGATTGGAAAATTATTGGACTTG ACCATGCTTGATACTCGTGAGTTAAAGTTTCGCTCATTCTTTGATTTTATGATTGGATTTATGAACGCGATTAAG GTCACTACAACCGTGATATTACTGATGTAATTTTGTTGTTTCTTCATTCGCTCAGGCTGACGTACGTTTATTCCT TAGCTTGGATACAACGCTGGTGAGTGATTCAACAGGAATTATCACCGAAAGTACTCACGAAGCATTTTCCGCATT GTGTTAGCTCGTAAGTAACTCAATTTCCGAGGCCTTGGTGAAATATTAACATTGGATAGTCGTTGAATCTCTCAG CAACGACGTCGAGCGTTCCATAATGGGACACAGCCAAGAAAACTGTACATTTCTCCGTCTCTGTCGTTAACTTGC TCTACTAAATGGTTCAGCAGGGCTCTCCGAAACGCTTTCCCAATCAAAATCCCGCGAAGCAATTTGGCGTGTTAT CGGGTCTGTATCTCAAGCACCACTTAAATCGCCAACTGGAACTTAGATACTTTGACAGCCAACAAGTGGTATTCA CACAGCTGCTGCAGAATACACGTAAGTCAGTCCAGTTGTAAATAAGTCCGATGGAAATAACTTGTCATAAATAGG ACTTGACTTCGTAGGCCCAACCTACTTGACCTTTGAGCTCATTGATCATGGTGACCACAGGATGCTTGGGATGGC TACCGCTCGAACAGAAATCGCATTCTTGACCAATTATACGGCAAAAATATTGACAATACGGTCATCTTATCAGGT GATAGTCATGCCAATTGGGTATCGGACCTCGCTCGTAAGTGATTATCCTTCTTCCCTTAAACCACTTCAAATTTA AACATGGTTAGACATCAATGACACGGAGACGTGAGTCGAGAACTAATCCATTGGATGATATATAAGCCTGACAAT TTATTAGATATGATCCACTGACAGGCAGTGGCGCAATCGGCGTCGAGTTTGCTGGGACCGGTGTCACATCTTCCT CCGGGACATTCAAACCAAATGGTCCTCTTGAATTTTCCAACAACGTCTCAATGTCCCTCGTACAAGAGAACATAG ACTTGCAATGGAGTGAAGGGCATTTCAGGGGATTCTTTACGATCCGGGTTACACCCGAATTTTTGAATGCCACTT ACTATTCAATGCAGAACGCTAGTAAGTTTTGTATACCCTCTCTCAAGTTTATTCCGTATATACGTTCACACTTTT TTTTCTTCAAGCTTTCCGGAATTTAGATGGTTTCGCATCTGCCAATTTTAGCGTTAAAGCTGGGGCTAACCGTTT GACGCGCCCAGTTGCCAATGGTAATATTCTTAGTGGTGCACTGAAACCTGGAAACCACTAG