Fungal Genomics

General Properties

Protein ID	AgabiH97|100110
Gene name
Location	scaffold_7:792570..795453
Strand	+
Gene length (bp)	2883
Transcript length (bp)	2184
Coding sequence length (bp)	2184
Protein length (aa)	728

Overview

Your browser does not support drawing a protein figure.

PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF01204	Trehalase	Trehalase	1.7E-103	79	524
PF01204	Trehalase	Trehalase	2.5E-08	634	680

Swissprot hits

GO

GO Term	Description	Terminal node
GO:0004555	alpha,alpha-trehalase activity	Yes
GO:0005991	trehalose metabolic process	Yes
GO:0044262	cellular carbohydrate metabolic process	No
GO:0044238	primary metabolic process	No
GO:0009311	oligosaccharide metabolic process	No
GO:0071704	organic substance metabolic process	No
GO:0009987	cellular process	No
GO:0008150	biological_process	No
GO:0003824	catalytic activity	No
GO:0015927	trehalase activity	No
GO:0044237	cellular metabolic process	No
GO:0016787	hydrolase activity	No
GO:0005984	disaccharide metabolic process	No
GO:0005975	carbohydrate metabolic process	No
GO:0003674	molecular_function	No
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	No
GO:0008152	metabolic process	No
GO:0016798	hydrolase activity, acting on glycosyl bonds	No

Deeploc

[Help with interpreting the results of Deeploc 2.0]

Localizations	Signals	Cytoplasm	Nucleus	Extracellular	Cell membrane	Mitochondrion	Plastid	Endoplasmic reticulum	Lysosome vacuole	Golgi apparatus	Peroxisome
Extracellular		0.1005	0.0474	0.7128	0.3317	0.0774	0.0038	0.4	0.3023	0.3084	0.0119

SignalP

SignalP signal predicted	Location	Score
Yes	1 - 21	0.999721

Transmembrane Domains

(None)

Transcription Factor Class

(None)

CAZymes

CAZyme category	E-value	Start	End
GH37	3.6E-125	79	524

Secondary Metabolism

(None)

Expression data

Analysis 1: Developmental stages of Agaricus bisporus (strain A15). Published in Pelkmans et al, Applied Microbiology and Biotechnology, 2016

Expression values

Label	Description	Expression (RPKM)	Confidence interval (low)	Confidence interval (high)
Casing	Casing mycelium	18.57	10.64	26.51
Initials	Initials knots	18.16	10.35	25.97
Pileal_Stipeal_center	Stage I stipe center	27.01	15.80	38.23
Pileal_Stipeal_shell	Stage I stipe shell	26.96	15.90	38.02
DIF_stipe_center	Stage II stipe center	25.45	14.90	35.99
DIF_stipe_shell	Stage II stipe shell	22.22	12.93	31.51
DIF_stipe_skin	Stage II stipe skin	19.75	11.35	28.14
DIF_cap_skin	Stage II cap skin	20.08	11.57	28.59
DIF_cap_tissue	Stage II cap tissue	25.26	14.81	35.71
DIF_gill_tissue	Stage II gill tissue	28.93	17.10	40.77
YFB_stipe_center	Young fruiting body stipe center	12.94	7.10	18.77
YFB_stipe_shell	Young fruiting body stipe shell	17.12	9.66	24.58
YFB_stipe_skin	Young fruiting body stipe skin	17.32	9.74	24.90
YFB_cap_skin	Young fruiting body cap skin	17.24	9.72	24.76
YFB_cap_tissue	Young fruiting body cap tissue	16.65	9.42	23.88
YFB_gill_tissue	Young fruiting body gill tissue	22.04	12.70	31.38
YFB_veil	Young fruiting body veil	25.50	14.94	36.06

Differential expression

Label1	Label2	Q-value	Significant difference
Casing	DIF_gill_tissue	0.124559	no
Casing	YFB_stipe_center	0.275539	no
Casing	YFB_stipe_shell	0.876733	no
Casing	YFB_stipe_skin	0.897415	no
Casing	YFB_cap_skin	0.892119	no
Casing	YFB_cap_tissue	0.824551	no
Casing	YFB_gill_tissue	0.688055	no
Casing	YFB_veil	0.340207	no
Casing	Initials	0.968440	no
Casing	Pileal_Stipeal_center	0.232528	no
Casing	Pileal_Stipeal_shell	0.224708	no
Casing	DIF_stipe_center	0.345193	no
Casing	DIF_stipe_shell	0.664562	no
Casing	DIF_stipe_skin	0.911254	no
Casing	DIF_cap_skin	0.883329	no
Casing	DIF_cap_tissue	0.367998	no
DIF_gill_tissue	YFB_stipe_center	0.004548	yes
DIF_gill_tissue	YFB_stipe_shell	0.064333	no
DIF_gill_tissue	YFB_stipe_skin	0.079305	no
DIF_gill_tissue	YFB_cap_skin	0.069952	no
DIF_gill_tissue	YFB_cap_tissue	0.051932	no
DIF_gill_tissue	YFB_gill_tissue	0.433061	no
DIF_gill_tissue	YFB_veil	0.774562	no
YFB_stipe_center	YFB_stipe_shell	0.457394	no
YFB_stipe_center	YFB_stipe_skin	0.438005	no
YFB_stipe_center	YFB_cap_skin	0.448483	no
YFB_stipe_center	YFB_cap_tissue	0.520132	no
YFB_stipe_center	YFB_gill_tissue	0.079829	no
YFB_stipe_center	YFB_veil	0.019987	yes
YFB_stipe_shell	YFB_stipe_skin	0.983338	no
YFB_stipe_shell	YFB_cap_skin	0.990426	no
YFB_stipe_shell	YFB_cap_tissue	0.963467	no
YFB_stipe_shell	YFB_gill_tissue	0.504935	no
YFB_stipe_shell	YFB_veil	0.204585	no
YFB_stipe_skin	YFB_cap_skin	0.993371	no
YFB_stipe_skin	YFB_cap_tissue	0.946416	no
YFB_stipe_skin	YFB_gill_tissue	0.530064	no
YFB_stipe_skin	YFB_veil	0.224708	no
YFB_cap_skin	YFB_cap_tissue	0.954453	no
YFB_cap_skin	YFB_gill_tissue	0.519036	no
YFB_cap_skin	YFB_veil	0.217207	no
YFB_cap_tissue	YFB_gill_tissue	0.442054	no
YFB_cap_tissue	YFB_veil	0.166190	no
YFB_gill_tissue	YFB_veil	0.741277	no
Initials	DIF_gill_tissue	0.106969	no
Initials	YFB_stipe_center	0.323698	no
Initials	YFB_stipe_shell	0.913951	no
Initials	YFB_stipe_skin	0.932887	no
Initials	YFB_cap_skin	0.927467	no
Initials	YFB_cap_tissue	0.865018	no
Initials	YFB_gill_tissue	0.631269	no
Initials	YFB_veil	0.296014	no
Initials	Pileal_Stipeal_center	0.202088	no
Initials	Pileal_Stipeal_shell	0.192529	no
Initials	DIF_stipe_center	0.301751	no
Initials	DIF_stipe_shell	0.606318	no
Initials	DIF_stipe_skin	0.870189	no
Initials	DIF_cap_skin	0.840169	no
Initials	DIF_cap_tissue	0.320650	no
Pileal_Stipeal_center	DIF_gill_tissue	0.897607	no
Pileal_Stipeal_center	YFB_stipe_center	0.009446	yes
Pileal_Stipeal_center	YFB_stipe_shell	0.129965	no
Pileal_Stipeal_center	YFB_stipe_skin	0.150164	no
Pileal_Stipeal_center	YFB_cap_skin	0.138429	no
Pileal_Stipeal_center	YFB_cap_tissue	0.104432	no
Pileal_Stipeal_center	YFB_gill_tissue	0.605922	no
Pileal_Stipeal_center	YFB_veil	0.914288	no
Pileal_Stipeal_center	Pileal_Stipeal_shell	0.996337	no
Pileal_Stipeal_center	DIF_stipe_center	0.910883	no
Pileal_Stipeal_center	DIF_stipe_shell	0.623881	no
Pileal_Stipeal_center	DIF_stipe_skin	0.354556	no
Pileal_Stipeal_center	DIF_cap_skin	0.390956	no
Pileal_Stipeal_center	DIF_cap_tissue	0.898413	no
Pileal_Stipeal_shell	DIF_gill_tissue	0.890188	no
Pileal_Stipeal_shell	YFB_stipe_center	0.009123	yes
Pileal_Stipeal_shell	YFB_stipe_shell	0.124412	no
Pileal_Stipeal_shell	YFB_stipe_skin	0.143064	no
Pileal_Stipeal_shell	YFB_cap_skin	0.131698	no
Pileal_Stipeal_shell	YFB_cap_tissue	0.099106	no
Pileal_Stipeal_shell	YFB_gill_tissue	0.598022	no
Pileal_Stipeal_shell	YFB_veil	0.914399	no
Pileal_Stipeal_shell	DIF_stipe_center	0.910530	no
Pileal_Stipeal_shell	DIF_stipe_shell	0.615446	no
Pileal_Stipeal_shell	DIF_stipe_skin	0.342918	no
Pileal_Stipeal_shell	DIF_cap_skin	0.378147	no
Pileal_Stipeal_shell	DIF_cap_tissue	0.898073	no
DIF_stipe_center	DIF_gill_tissue	0.773829	no
DIF_stipe_center	YFB_stipe_center	0.016104	yes
DIF_stipe_center	YFB_stipe_shell	0.208106	no
DIF_stipe_center	YFB_stipe_skin	0.228037	no
DIF_stipe_center	YFB_cap_skin	0.221032	no
DIF_stipe_center	YFB_cap_tissue	0.168405	no
DIF_stipe_center	YFB_gill_tissue	0.744180	no
DIF_stipe_center	YFB_veil	0.996972	no
DIF_stipe_center	DIF_stipe_shell	0.757220	no
DIF_stipe_center	DIF_stipe_skin	0.484796	no
DIF_stipe_center	DIF_cap_skin	0.524584	no
DIF_stipe_center	DIF_cap_tissue	0.988715	no
DIF_stipe_shell	DIF_gill_tissue	0.449066	no
DIF_stipe_shell	YFB_stipe_center	0.067147	no
DIF_stipe_shell	YFB_stipe_shell	0.477236	no
DIF_stipe_shell	YFB_stipe_skin	0.510996	no
DIF_stipe_shell	YFB_cap_skin	0.497259	no
DIF_stipe_shell	YFB_cap_tissue	0.413717	no
DIF_stipe_shell	YFB_gill_tissue	0.988715	no
DIF_stipe_shell	YFB_veil	0.753958	no
DIF_stipe_shell	DIF_stipe_skin	0.803499	no
DIF_stipe_shell	DIF_cap_skin	0.838904	no
DIF_stipe_shell	DIF_cap_tissue	0.774007	no
DIF_stipe_skin	DIF_gill_tissue	0.215162	no
DIF_stipe_skin	YFB_stipe_center	0.185817	no
DIF_stipe_skin	YFB_stipe_shell	0.753475	no
DIF_stipe_skin	YFB_stipe_skin	0.781835	no
DIF_stipe_skin	YFB_cap_skin	0.772428	no
DIF_stipe_skin	YFB_cap_tissue	0.690614	no
DIF_stipe_skin	YFB_gill_tissue	0.822818	no
DIF_stipe_skin	YFB_veil	0.480687	no
DIF_stipe_skin	DIF_cap_skin	0.977733	no
DIF_stipe_skin	DIF_cap_tissue	0.507062	no
DIF_cap_skin	DIF_gill_tissue	0.243628	no
DIF_cap_skin	YFB_stipe_center	0.165674	no
DIF_cap_skin	YFB_stipe_shell	0.717734	no
DIF_cap_skin	YFB_stipe_skin	0.749406	no
DIF_cap_skin	YFB_cap_skin	0.737527	no
DIF_cap_skin	YFB_cap_tissue	0.654377	no
DIF_cap_skin	YFB_gill_tissue	0.856936	no
DIF_cap_skin	YFB_veil	0.522288	no
DIF_cap_skin	DIF_cap_tissue	0.547916	no
DIF_cap_tissue	DIF_gill_tissue	0.762351	no
DIF_cap_tissue	YFB_stipe_center	0.021322	yes
DIF_cap_tissue	YFB_stipe_shell	0.227705	no
DIF_cap_tissue	YFB_stipe_skin	0.250056	no
DIF_cap_tissue	YFB_cap_skin	0.241805	no
DIF_cap_tissue	YFB_cap_tissue	0.187911	no
DIF_cap_tissue	YFB_gill_tissue	0.765212	no
DIF_cap_tissue	YFB_veil	0.986394	no

Orthologs

Orthofinder run ID	1
Orthogroup	4843
Change Orthofinder run	1: Agaricomycetes 5: Agaricus - all strains

Species	Protein ID
Agaricus bisporus var bisporus H39	AgabiH39|100110
Agaricus bisporus var bisporus H97	AgabiH97|100110 (this protein)
Rhodonia placenta FPRL280	RhoplFPRL280|970_1

Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus Download genbank file of locus (reverse complement) The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>AgabiH97|100110 MVSLAALVQLACLLRAANVLAQESTASPTLTLSTAIPSATAPTSSELPSQVPVPPKQAWCPSEIFCPGELLQTVN IAHLFPDDKTFADKPTSKSSQEVLAAFRNITNDTTYGQIIDFVEQNFGGEGRELEALTVPDFDPNPSFLQNVTNP LVQAWSKIVHGYWTQLIRGTNSSNLCGSDSECESSLIPLNHTFVVPGGRFREQYYWDSFWIVEGLLESQLYSVVN STLQNFMDELDTIGFIPNGGRIYYLDRSQPPLFIQMLDRYVKVTGDRSILDRALPLAEKELTWWHDNRSLNVTSP FTNQTHLVYHYSVNNSAPRPESYLTDYMTANDPDAPTLNDTQKADLYAELASGAETGWDYTMRWFKDGANDGSLR NLGIRDLVAVDLNSILYRNHIALADLYGSSNDQQADTHRSAAADLHTAVLDLMWDNDKLAFYDFNLATNKRKNFF STANFYPVWSGIIPDALLQDSKAAFGHFASVNLVLSRYNGTFPTTFVNTGQQWDGPNTWAPHQYIILQALRALPA NVSSGNAPSTEDGQSSWDLIPSGQLGMEESQLPPQPSNTGNITNTGPNADINVGNGTVVNGGNATSGEGWSVMLQ RELANRYITSAFCSWVATGGSIEGMVPRLSPELLNITQSVNNTGNMFEKFSAFDVDSAGRGGEYTVQAGFGWTNG LVMWIASNYGNMLNAPECPLITLEDAQTSSASLNASPVMWTVAIAMAITALL*
Coding	>AgabiH97|100110 ATGGTGTCTTTGGCTGCTCTTGTTCAACTAGCGTGTCTGCTCCGAGCAGCAAATGTCCTGGCTCAGGAATCAACT GCTTCCCCAACTCTTACTCTATCGACTGCGATCCCGTCTGCCACTGCGCCTACAAGTTCAGAGCTTCCTTCTCAA GTACCTGTGCCACCGAAACAAGCGTGGTGTCCCTCTGAAATCTTCTGTCCCGGAGAACTTTTGCAGACTGTCAAT ATTGCTCACCTGTTCCCTGATGATAAAACTTTTGCCGACAAGCCGACGAGCAAGTCTTCGCAAGAAGTACTAGCC GCCTTCCGGAATATTACGAATGATACTACCTACGGTCAGATCATTGACTTTGTGGAGCAAAACTTTGGTGGTGAA GGTCGCGAACTCGAAGCCCTTACCGTACCGGACTTCGATCCAAATCCCTCGTTTTTGCAAAACGTCACGAATCCC TTGGTTCAGGCTTGGTCGAAGATTGTCCATGGATATTGGACTCAACTCATCCGGGGCACTAACTCCTCTAATCTC TGTGGTTCTGATTCCGAATGCGAGAGCTCTCTCATTCCGTTGAACCATACCTTTGTTGTTCCAGGTGGTCGCTTC AGGGAACAATACTACTGGGACAGTTTCTGGATCGTGGAGGGCCTTCTTGAGTCTCAACTTTACTCTGTCGTAAAC AGCACGCTCCAGAATTTCATGGATGAGCTCGATACCATTGGATTTATTCCCAATGGAGGTCGAATTTATTATTTG GACCGATCGCAGCCTCCACTCTTCATTCAGATGCTTGATCGCTACGTCAAAGTCACCGGAGATAGGTCGATCTTG GACAGAGCTCTTCCTCTCGCAGAGAAAGAACTCACTTGGTGGCACGATAACCGTTCACTGAACGTCACGAGCCCT TTCACAAACCAGACTCACCTTGTATACCACTATAGCGTGAATAACAGTGCCCCCCGCCCCGAATCTTATTTGACT GATTATATGACCGCCAATGATCCTGATGCTCCTACCCTTAACGATACCCAAAAGGCTGATCTGTATGCTGAACTC GCTTCTGGCGCCGAAACAGGTTGGGATTATACCATGAGATGGTTTAAAGACGGAGCCAATGATGGTTCCTTAAGA AATCTTGGTATTCGTGATTTAGTCGCTGTAGACCTGAACAGTATCCTATACCGCAATCACATTGCACTCGCAGAT CTATATGGTTCCTCGAATGACCAACAAGCCGATACTCACCGTTCAGCTGCTGCTGACCTTCATACTGCAGTGCTG GATCTTATGTGGGATAATGACAAACTCGCTTTTTACGACTTCAACCTCGCTACAAACAAAAGGAAGAACTTCTTT AGTACTGCAAATTTCTATCCTGTATGGAGCGGCATCATACCAGACGCACTTCTACAAGATAGCAAAGCGGCCTTC GGCCATTTCGCATCGGTCAATCTTGTGCTCAGTCGTTACAATGGTACATTTCCCACCACTTTTGTTAACACTGGC CAGCAGTGGGATGGGCCCAATACTTGGGCGCCGCATCAATATATAATACTTCAGGCCTTACGTGCCTTGCCTGCC AACGTCAGCTCAGGCAATGCTCCTTCGACAGAGGATGGTCAGTCGAGCTGGGATCTAATTCCGTCCGGTCAACTT GGCATGGAAGAGAGTCAACTTCCACCACAACCCTCCAATACCGGTAATATCACTAATACTGGTCCGAACGCCGAT ATCAATGTCGGGAACGGCACCGTCGTCAATGGTGGCAATGCAACATCTGGGGAGGGTTGGAGTGTTATGCTCCAA AGAGAACTTGCCAATAGGTATATCACGAGTGCTTTCTGTAGCTGGGTGGCGACGGGAGGCTCCATTGAAGGCATG GTACCAAGGCTTTCGCCTGAATTGTTGAATATTACTCAGAGCGTCAACAACACCGGAAACATGTTTGAGAAATTC TCTGCTTTTGATGTCGATTCGGCTGGTAGAGGTGGAGAATATACTGTCCAGGCCGGGTTTGGATGGACTAATGGT CTCGTCATGTGGATCGCTAGCAACTACGGTAATATGCTTAATGCTCCTGAATGCCCGCTTATCACTCTCGAGGAT GCCCAAACGTCGTCGGCGAGTCTCAATGCGTCTCCTGTCATGTGGACTGTGGCCATAGCAATGGCTATCACTGCT CTTCTGTGA
Transcript	>AgabiH97|100110 ATGGTGTCTTTGGCTGCTCTTGTTCAACTAGCGTGTCTGCTCCGAGCAGCAAATGTCCTGGCTCAGGAATCAACT GCTTCCCCAACTCTTACTCTATCGACTGCGATCCCGTCTGCCACTGCGCCTACAAGTTCAGAGCTTCCTTCTCAA GTACCTGTGCCACCGAAACAAGCGTGGTGTCCCTCTGAAATCTTCTGTCCCGGAGAACTTTTGCAGACTGTCAAT ATTGCTCACCTGTTCCCTGATGATAAAACTTTTGCCGACAAGCCGACGAGCAAGTCTTCGCAAGAAGTACTAGCC GCCTTCCGGAATATTACGAATGATACTACCTACGGTCAGATCATTGACTTTGTGGAGCAAAACTTTGGTGGTGAA GGTCGCGAACTCGAAGCCCTTACCGTACCGGACTTCGATCCAAATCCCTCGTTTTTGCAAAACGTCACGAATCCC TTGGTTCAGGCTTGGTCGAAGATTGTCCATGGATATTGGACTCAACTCATCCGGGGCACTAACTCCTCTAATCTC TGTGGTTCTGATTCCGAATGCGAGAGCTCTCTCATTCCGTTGAACCATACCTTTGTTGTTCCAGGTGGTCGCTTC AGGGAACAATACTACTGGGACAGTTTCTGGATCGTGGAGGGCCTTCTTGAGTCTCAACTTTACTCTGTCGTAAAC AGCACGCTCCAGAATTTCATGGATGAGCTCGATACCATTGGATTTATTCCCAATGGAGGTCGAATTTATTATTTG GACCGATCGCAGCCTCCACTCTTCATTCAGATGCTTGATCGCTACGTCAAAGTCACCGGAGATAGGTCGATCTTG GACAGAGCTCTTCCTCTCGCAGAGAAAGAACTCACTTGGTGGCACGATAACCGTTCACTGAACGTCACGAGCCCT TTCACAAACCAGACTCACCTTGTATACCACTATAGCGTGAATAACAGTGCCCCCCGCCCCGAATCTTATTTGACT GATTATATGACCGCCAATGATCCTGATGCTCCTACCCTTAACGATACCCAAAAGGCTGATCTGTATGCTGAACTC GCTTCTGGCGCCGAAACAGGTTGGGATTATACCATGAGATGGTTTAAAGACGGAGCCAATGATGGTTCCTTAAGA AATCTTGGTATTCGTGATTTAGTCGCTGTAGACCTGAACAGTATCCTATACCGCAATCACATTGCACTCGCAGAT CTATATGGTTCCTCGAATGACCAACAAGCCGATACTCACCGTTCAGCTGCTGCTGACCTTCATACTGCAGTGCTG GATCTTATGTGGGATAATGACAAACTCGCTTTTTACGACTTCAACCTCGCTACAAACAAAAGGAAGAACTTCTTT AGTACTGCAAATTTCTATCCTGTATGGAGCGGCATCATACCAGACGCACTTCTACAAGATAGCAAAGCGGCCTTC GGCCATTTCGCATCGGTCAATCTTGTGCTCAGTCGTTACAATGGTACATTTCCCACCACTTTTGTTAACACTGGC CAGCAGTGGGATGGGCCCAATACTTGGGCGCCGCATCAATATATAATACTTCAGGCCTTACGTGCCTTGCCTGCC AACGTCAGCTCAGGCAATGCTCCTTCGACAGAGGATGGTCAGTCGAGCTGGGATCTAATTCCGTCCGGTCAACTT GGCATGGAAGAGAGTCAACTTCCACCACAACCCTCCAATACCGGTAATATCACTAATACTGGTCCGAACGCCGAT ATCAATGTCGGGAACGGCACCGTCGTCAATGGTGGCAATGCAACATCTGGGGAGGGTTGGAGTGTTATGCTCCAA AGAGAACTTGCCAATAGGTATATCACGAGTGCTTTCTGTAGCTGGGTGGCGACGGGAGGCTCCATTGAAGGCATG GTACCAAGGCTTTCGCCTGAATTGTTGAATATTACTCAGAGCGTCAACAACACCGGAAACATGTTTGAGAAATTC TCTGCTTTTGATGTCGATTCGGCTGGTAGAGGTGGAGAATATACTGTCCAGGCCGGGTTTGGATGGACTAATGGT CTCGTCATGTGGATCGCTAGCAACTACGGTAATATGCTTAATGCTCCTGAATGCCCGCTTATCACTCTCGAGGAT GCCCAAACGTCGTCGGCGAGTCTCAATGCGTCTCCTGTCATGTGGACTGTGGCCATAGCAATGGCTATCACTGCT CTTCTGTGA
Gene	>AgabiH97|100110 ATGGTGTCTTTGGCTGCTCTTGTTCAACTAGCGTGTCTGCTCCGAGCAGCAAATGTCCTGGCTCAGGAATCAACT GCTTCCCCAACTCTTACTCTATCGACTGCGATCCCGTCTGCCACTGCGCCTACAAGTTCAGAGCTTCCTTCTCAA GTACCTGTGCCACCGAAACAAGCGTGGTGTCCCTCTGAAATCTTCTGTCCCGGAGAAGTGGGTATTATTGCTCTT ATATGATCCATTACTGACTGCCATCTCTTCCTTTCTCAGCTTTTGCAGACTGTCAATATTGCTCACCTGTTCCCT GATGATAAAACTTTTGCCGACAAGGTAATGAATCATTGCTTCTCAACATCTGTGTGCTAATGTACCAACTTAATC CACAAGCCGACGAGCAAGTCTTCGCAAGAAGTACTAGCCGCCTTCCGGAATATTACGAATGATACTACCTACGGT CAGATCATTGACTTTGTGGAGCAAAACTTTGGTGGTGAAGGTCGCGAACTCGAAGCCCTTACCGTACCGGACTTC GATCCAAATCCCTCGTTTTTGCAAAACGTCACGAATCCCTTGGTTCAGGCTTGGTCGAAGATTGTCCATGGATAT TGGACTCAACTCATCCGGGGCACTAACTCCTCTAATCTCTGTGGTTCTGATTCCGAATGCGAGAGCTCTCTCATT CCGTTGAACCATACCTTTGTTGTTCCAGGTGAGTTCGGCATCTTACTGGTTTCATGGAGATGTTTTGACGACTTC GCCTTAGGTGGTCGCTTCAGGGAACAATACTACTGGGACAGTTTCTGGATCGTGGAGGGCCTTCTTGAGTCTCAA CTTTACTCTGTCGTAAACAGCACGCTCCAGAATTTCATGGATGAGCTCGATACCATTGGATTTATTCCCAATGGA GGTCGAATTTATTGTTAGTAACATTTCTATAATCTCAGTACCCTACTTACGGTGATTCACCTCCAGATTTGGACC GATCGCAGCCTCCACTCTTCATTCAGATGCTTGATCGCTACGTCAAAGTCACCGGAGATAGGTCGATCTTGGACA GAGCTCTTCCTCTCGCAGAGGTATCTATTCCTAATACGTCGTTCATCTTTGACATGCTGAATGTTATATAGAAAG AACTCACTTGGTGGCACGATAACCGTTCACTGAACGTCACGAGCCCTTTCACAAACCAGACTCACCTTGTATACC ACTATAGCGTGAATAACAGTGCCCCCCGCCCCGAATCTTATTTGACTGGTAAGCCATCTTTTTCTCAATTGGGAC GCCGTCGGATTGTTAAAATGTTTGATACAGATTATATGACCGCCAATGATCCTGATGCTCCTACCCTTAACGATA CCCAAAAGGCTGATCTGTATGCTGAACTCGCTTCTGGCGCCGAAACAGGTGGGTACACGGGAAGTTTATATATCT TGATATCGGCCCTGATGCAGATCTAGGTTGGGATTATACCATGAGATGGTTTAAAGACGGAGCCAATGATGGTTC CTTAAGAAATCTTGGTATTCGTGATTTAGTCGCTGTAGACCTGAACAGTATCCTATGTGAGACGGAATATTTTCT TATCGACTCAAAATCTGACATTTCTTGCAGACCGCAATCACATTGCACTCGCAGATCTATATGGTTCCTCGAATG ACCAACAAGCCGATACTCACCGTTCAGCTGCTGCTGACCTTCATACTGCAGTGCTGGATCTTATGTGGGATAATG ACAAAGTGAGGGCACCCCTCCAAACGTAAAGATTATAACTCGAGGCTTAACCGAAACAGCTCGCTTTTTACGACT TCAACCTCGCTACAAACAAAAGGAAGAACTTCTTTAGTACTGCAAATTTCTATCCTGTATGGAGCGGCATCATAC CAGACGCACTTCTACAAGATAGCAAAGCGGCCTTCGGCCATTTCGCATCGGTCAATCTTGTGCTCAGTCGTTACA ATGGTACATTTCCCACCACTTTTGTTAACACTGGCCAGCAGTGGTAAGTGTGATATATGACTTTAACGCTTTGTT CGATGCTGTACTTAATGGCCTTTAGGGATGGGCCCAATACTTGGGCGCCGCATCAATATATAATACTTCAGGCCT TACGTGCCTTGCCTGCCAACGTCAGCTCAGGCAATGCTCCTTCGACAGAGGATGGTCAGTCGAGCTGGGATCTAA TTCCGTCCGGTCAACTTGGCATGGAAGAGAGTCAACTTCCACCACAACCCTCCAATACCGGTAATATCACTAATA CTGGTCCGAACGCCGATATCAATGTCGGGAACGGCACCGTCGTCAATGGTGGCAATGCAACATCTGGGGAGGGTT GGAGTGTTATGCTCCAAAGAGAACTTGCCAATAGGTATATCACGAGTGCTTTCTGTAGCTGGTGCGTAGTAGAAG CTCCTACTGTGTGCCTTTGGTTAACAGCAACCGTATAGGGTGGCGACGGGAGGCTCCATTGAAGGCATGGTACCA AGGCTTTCGCCTGAATTGTTGAATATTACTCAGAGCGTCAACAACACCGGAAACGTACGCGTCCAGCTCATAATG CCAATTTTTCTTTTTGCTCATTCCCAGATAAGATGTTTGAGAAATTCTCTGCTTTTGATGTCGATTCGGCTGGTA GAGGTGGAGAATATACTGTCCAGGTAGGTTCTCGACGCCCGTATGATAACTTCATGTAATTGATATTTCTTACAG GCCGGGTTTGGATGGACTAATGGTCTCGTCATGTGGATCGCTAGCAACTACGGTAATATGCTTAATGCTCCTGAA TGCCCGCTTATCACTCTCGAGGATGCCCAAACGTCGTCGGCGAGTCTCAATGCGTCTCCTGTCATGTGGACTGTG GCCATAGCAATGGCTATCACTGCTCTTCTGTGA