Fungal Genomics

Agaricus bisporus var bisporus H39 Agaricus bisporus var bisporus H97 Agaricus bisporus var burnettii H119p4 Aspergillus niger CBS 112.32 Aspergillus niger CBS 113.50 Aspergillus niger CBS 115988 Aspergillus niger CBS 115989 Aspergillus niger CBS 118.52 Aspergillus niger CBS 124.48 Aspergillus niger CBS 131.52 Aspergillus niger CBS 133816 Aspergillus niger CBS 147320 Aspergillus niger CBS 147321 Aspergillus niger CBS 147322 Aspergillus niger CBS 147323 Aspergillus niger CBS 147324 Aspergillus niger CBS 147343 Aspergillus niger CBS 147344 Aspergillus niger CBS 147345 Aspergillus niger CBS 147346 Aspergillus niger CBS 147347 Aspergillus niger CBS 147352 Aspergillus niger CBS 147353 Aspergillus niger CBS 147371 Aspergillus niger CBS 147482 Aspergillus niger CBS 630.78 Aspergillus niger CBS 769.97 Niveomyces coronatus CBS 149186 Ophiocordyceps australis 1348a (Ghana) Ophiocordyceps australis map64 (Brazil) Ophiocordyceps camponoti-floridani Ophiocordyceps camponoti-rufipedis Ophiocordyceps kimflemingae Ophiocordyceps subramaniannii Penicillium roqueforti DTO002I6 Penicillium roqueforti DTO003C3 Penicillium roqueforti DTO003H1 (CBS 147308) Penicillium roqueforti DTO006G1 Penicillium roqueforti DTO006G7 Penicillium roqueforti DTO012A1 Penicillium roqueforti DTO012A2 (CBS 147309) Penicillium roqueforti DTO012A6 (CBS 147310) Penicillium roqueforti DTO012A7 Penicillium roqueforti DTO012A8 Penicillium roqueforti DTO012A9 Penicillium roqueforti DTO013E5 Penicillium roqueforti DTO013F2 Penicillium roqueforti DTO013F5 (CBS 147311) Penicillium roqueforti DTO027I6 Penicillium roqueforti DTO032C6 Penicillium roqueforti DTO039G3 Penicillium roqueforti DTO046C5 Penicillium roqueforti DTO070G2 (CBS 147317) Penicillium roqueforti DTO081F9 (CBS 147318) Penicillium roqueforti DTO101D6 (CBS 147325) Penicillium roqueforti DTO102I9 (CBS 147326) Penicillium roqueforti DTO126G2 (CBS 147330) Penicillium roqueforti DTO127F7 (CBS 147331) Penicillium roqueforti DTO127F9 (CBS 147332) Penicillium roqueforti DTO130C1 (CBS 147333) Penicillium roqueforti DTO163C3 (CBS 147337) Penicillium roqueforti DTO163F5 (CBS 147338) Penicillium roqueforti DTO163G4 (CBS 147339) Penicillium roqueforti DTO265D5 (CBS 147372) Penicillium roqueforti DTO369A1 (CBS 147354) Penicillium roqueforti DTO375B1 (CBS 147355) Penicillium roqueforti DTO377G2 (LCP 96.3914a) Penicillium roqueforti LCP96 04111 Rhodonia placenta FPRL280 Torrubiellomyces zombiae CBS 149187

General Properties

Protein ID	AgabiH97|094790
Gene name
Location	scaffold_6:1675852..1678683
Strand	-
Gene length (bp)	2831
Transcript length (bp)	2610
Coding sequence length (bp)	2610
Protein length (aa)	870

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PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF08590	DUF1771	Domain of unknown function (DUF1771)	2.5E-16	662	724
PF14608	zf-CCCH_2	RNA-binding, Nab2-type zinc finger	2.8E-03	492	509
PF14608	zf-CCCH_2	RNA-binding, Nab2-type zinc finger	4.2E-01	515	534

Swissprot hits

Swissprot ID	Swissprot Description	Start	End	E-value
sp|Q6ZPZ3|ZC3H4_MOUSE	Zinc finger CCCH domain-containing protein 4 OS=Mus musculus GN=Zc3h4 PE=1 SV=2	486	534	3.0E-09
sp|Q9UPT8|ZC3H4_HUMAN	Zinc finger CCCH domain-containing protein 4 OS=Homo sapiens GN=ZC3H4 PE=1 SV=3	486	534	3.0E-09
sp|Q8N5P1|ZC3H8_HUMAN	Zinc finger CCCH domain-containing protein 8 OS=Homo sapiens GN=ZC3H8 PE=1 SV=2	465	535	1.0E-07
sp|Q9JJ48|ZC3H8_MOUSE	Zinc finger CCCH domain-containing protein 8 OS=Mus musculus GN=Zc3h8 PE=1 SV=2	487	534	1.0E-07
sp|A6NMK7|CPS4L_HUMAN	Putative cleavage and polyadenylation specificity factor subunit 4-like protein OS=Homo sapiens GN=CPSF4L PE=2 SV=3	486	535	6.0E-06

GO

(None)

SignalP

[Help with interpreting these statistics]

SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
No	1 - 24	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Developmental stages of Agaricus bisporus (strain A15). Published in Pelkmans et al, Applied Microbiology and Biotechnology, 2016

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Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>AgabiH97|094790 MATLAVPSSYTPLFPTETPHAHDSATLKFPHTRAQLAAVARQHKPADTHDSDTGTDDLGRVPSSFVAKVVSLLTD EKEDELKALLKATYSLDNQSLEESVLDLMHKHRDDVAGVPFVFLTPTRRPISRPSSRASSHSARLHPARPDTPSS APPSPLAWSHRRPHTPATSPLANGEPRSYMSPKSDYSPSSSPILAHAQATQAQAQWTASLPASPLLTSRSLNAKA SEFRPIPRPLSSASSQPGSLLRADTPSPDMWAHNSPRATSNLAIAAPLIADQSTSAPRALTPSSSLRSFVRPDED EDEGEDPDPYDPFTNNGQLPAPFHSITVSDFDNQWSVSPPEESELTHMATQPPYEYGYSPFNPNGNNDPIDDVEA NAALTDGMTPFDVLSSVFGSTLAPSELEEALAANGYDFEHAMAWLIDRGLPQQPASPTQVRMQPMGNRVTLVSRD TGGTIRGTVKGGYHNNNSNGTGRGAPPRYANGRPSQSGNRVCRYFVAGECLRADCRFSHDLERALCRFWLRGMCA KNEACEFLHHLPKDVDVANLNVAMSRANIGPPSHYGPPEMHAPVDEFPVLGYEGNVMRMNKPGGYYDPGRTRFAA AVKKPAPPKINGVQLARVMGPAADNLHHQSAIVAPRPSPRIKLHPPTLVPTLPTGDAVNQLYMNYRQRALQLGAA RNACLSRAADAWRRGDGAAAKRFSREGHDLNAKMSTEMANAASKLVRERSSLAEQAVRARDLSWSDDPGDRSARG RPCGSGLGVVLGVGRKDVGDGKLTTEERTECMLDLHGLHSNEATEVLEQFLLSLEREHFYGLAYAVVGEEKHTGT QDPSRGASRNRLATAVREWVHRWGYPWSERDGIICIDPLTHASE*
Coding	>AgabiH97|094790 ATGGCTACCCTCGCCGTCCCTTCCTCTTACACGCCCCTCTTTCCGACAGAGACACCACACGCACACGACTCTGCC ACCCTCAAGTTTCCCCACACAAGAGCTCAGCTCGCCGCCGTCGCGCGCCAACACAAACCAGCCGACACTCATGAT TCAGACACCGGCACAGACGACCTCGGCCGCGTTCCGTCTTCCTTTGTCGCAAAGGTCGTTTCTCTGCTCACAGAC GAAAAGGAAGACGAGCTCAAGGCGCTGCTCAAGGCCACCTACAGTCTAGACAATCAATCACTCGAAGAGTCTGTG CTGGACCTCATGCACAAGCACCGCGACGACGTCGCTGGCGTCCCATTTGTATTTTTGACGCCAACGCGTCGTCCC ATTTCCCGTCCTTCTTCCCGGGCGTCATCTCACTCGGCCCGTCTCCACCCTGCACGCCCAGACACGCCCTCTTCC GCCCCACCTTCTCCCCTTGCCTGGTCACATCGTCGCCCTCACACCCCTGCCACCTCCCCACTCGCAAACGGCGAG CCAAGGTCATACATGTCCCCAAAGAGCGATTATTCTCCCTCATCGTCCCCTATCTTGGCCCACGCTCAAGCCACT CAAGCACAGGCCCAATGGACTGCCAGTCTTCCAGCCTCTCCTTTGCTCACCTCTCGTTCTCTCAATGCCAAAGCT TCAGAATTCAGACCCATCCCAAGGCCGCTTTCTTCAGCTTCTTCTCAACCTGGCTCACTTCTTCGTGCCGACACG CCTTCTCCAGACATGTGGGCTCACAACTCCCCACGCGCCACCTCCAATCTCGCCATCGCCGCGCCACTCATAGCA GATCAGTCCACTTCCGCTCCAAGGGCACTCACCCCCAGCTCATCTTTGCGCTCCTTTGTCCGCCCCGATGAAGAC GAAGACGAAGGCGAAGATCCCGATCCCTATGATCCCTTCACTAACAATGGCCAGCTGCCAGCCCCGTTCCATTCC ATTACCGTTTCCGACTTTGATAATCAGTGGTCCGTATCACCGCCAGAAGAGTCAGAGCTCACTCATATGGCAACT CAACCTCCATACGAGTACGGCTATTCGCCGTTCAACCCGAATGGAAACAATGATCCCATCGACGATGTTGAAGCC AACGCCGCTCTCACAGATGGCATGACGCCCTTTGATGTTCTCAGTTCCGTCTTTGGCTCTACACTAGCCCCATCA GAGCTCGAAGAGGCTCTGGCCGCCAATGGATACGATTTCGAGCACGCGATGGCGTGGTTGATCGATCGTGGTTTA CCTCAACAGCCAGCGTCGCCTACACAAGTACGAATGCAGCCTATGGGCAATCGTGTCACTCTGGTATCACGCGAT ACCGGGGGAACCATACGTGGCACTGTCAAAGGCGGATATCACAACAACAATAGCAATGGGACCGGTCGTGGTGCT CCTCCGAGATATGCCAATGGCCGCCCTTCCCAATCGGGTAATAGGGTCTGCAGATATTTTGTTGCTGGAGAATGC CTCCGCGCGGACTGCCGTTTCAGTCATGACTTGGAGCGCGCCTTGTGTCGCTTTTGGTTGAGAGGTATGTGTGCT AAAAATGAAGCTTGCGAGTTTTTGCACCATTTGCCCAAGGATGTGGATGTGGCAAACTTGAACGTGGCGATGTCC CGAGCGAATATAGGACCACCCAGTCACTATGGTCCACCCGAGATGCACGCTCCCGTTGACGAGTTCCCTGTACTT GGGTATGAGGGAAATGTTATGAGGATGAATAAACCTGGAGGATATTATGATCCGGGACGAACACGATTCGCAGCT GCTGTCAAGAAACCAGCTCCTCCCAAGATTAATGGCGTTCAACTGGCTCGTGTGATGGGCCCGGCCGCGGATAAT TTGCATCACCAATCGGCTATTGTTGCTCCTCGACCGTCGCCACGTATAAAACTTCATCCACCTACCTTGGTACCG ACTTTACCTACCGGTGATGCTGTCAATCAGTTATACATGAATTATCGTCAACGTGCGTTACAGTTGGGAGCGGCA AGGAATGCTTGTTTGTCTCGTGCTGCTGATGCATGGAGACGAGGAGATGGCGCTGCTGCCAAGAGGTTTAGTAGG GAAGGACATGATCTGAATGCCAAAATGTCAACGGAGATGGCGAATGCGGCATCTAAATTAGTTAGGGAACGATCG AGTTTGGCGGAACAGGCTGTGAGGGCGAGAGATTTAAGTTGGTCGGATGATCCTGGGGACCGGAGTGCGAGGGGG AGACCTTGTGGGTCTGGTTTGGGTGTTGTTTTGGGTGTGGGGAGGAAGGATGTTGGAGATGGCAAGTTGACGACG GAGGAGAGGACGGAGTGTATGCTTGATTTACATGGTTTGCATTCGAATGAGGCTACGGAGGTGTTGGAACAATTT TTACTTTCGTTGGAAAGGGAACACTTTTATGGTTTAGCTTATGCGGTTGTTGGAGAAGAAAAGCATACTGGGACG CAAGATCCTTCTAGGGGCGCTTCGCGTAATAGATTGGCTACGGCTGTACGTGAATGGGTTCATCGTTGGGGATAT CCATGGAGCGAGAGAGATGGTATCATTTGCATTGATCCATTGACCCACGCGTCCGAGTAG
Transcript	>AgabiH97|094790 ATGGCTACCCTCGCCGTCCCTTCCTCTTACACGCCCCTCTTTCCGACAGAGACACCACACGCACACGACTCTGCC ACCCTCAAGTTTCCCCACACAAGAGCTCAGCTCGCCGCCGTCGCGCGCCAACACAAACCAGCCGACACTCATGAT TCAGACACCGGCACAGACGACCTCGGCCGCGTTCCGTCTTCCTTTGTCGCAAAGGTCGTTTCTCTGCTCACAGAC GAAAAGGAAGACGAGCTCAAGGCGCTGCTCAAGGCCACCTACAGTCTAGACAATCAATCACTCGAAGAGTCTGTG CTGGACCTCATGCACAAGCACCGCGACGACGTCGCTGGCGTCCCATTTGTATTTTTGACGCCAACGCGTCGTCCC ATTTCCCGTCCTTCTTCCCGGGCGTCATCTCACTCGGCCCGTCTCCACCCTGCACGCCCAGACACGCCCTCTTCC GCCCCACCTTCTCCCCTTGCCTGGTCACATCGTCGCCCTCACACCCCTGCCACCTCCCCACTCGCAAACGGCGAG CCAAGGTCATACATGTCCCCAAAGAGCGATTATTCTCCCTCATCGTCCCCTATCTTGGCCCACGCTCAAGCCACT CAAGCACAGGCCCAATGGACTGCCAGTCTTCCAGCCTCTCCTTTGCTCACCTCTCGTTCTCTCAATGCCAAAGCT TCAGAATTCAGACCCATCCCAAGGCCGCTTTCTTCAGCTTCTTCTCAACCTGGCTCACTTCTTCGTGCCGACACG CCTTCTCCAGACATGTGGGCTCACAACTCCCCACGCGCCACCTCCAATCTCGCCATCGCCGCGCCACTCATAGCA GATCAGTCCACTTCCGCTCCAAGGGCACTCACCCCCAGCTCATCTTTGCGCTCCTTTGTCCGCCCCGATGAAGAC GAAGACGAAGGCGAAGATCCCGATCCCTATGATCCCTTCACTAACAATGGCCAGCTGCCAGCCCCGTTCCATTCC ATTACCGTTTCCGACTTTGATAATCAGTGGTCCGTATCACCGCCAGAAGAGTCAGAGCTCACTCATATGGCAACT CAACCTCCATACGAGTACGGCTATTCGCCGTTCAACCCGAATGGAAACAATGATCCCATCGACGATGTTGAAGCC AACGCCGCTCTCACAGATGGCATGACGCCCTTTGATGTTCTCAGTTCCGTCTTTGGCTCTACACTAGCCCCATCA GAGCTCGAAGAGGCTCTGGCCGCCAATGGATACGATTTCGAGCACGCGATGGCGTGGTTGATCGATCGTGGTTTA CCTCAACAGCCAGCGTCGCCTACACAAGTACGAATGCAGCCTATGGGCAATCGTGTCACTCTGGTATCACGCGAT ACCGGGGGAACCATACGTGGCACTGTCAAAGGCGGATATCACAACAACAATAGCAATGGGACCGGTCGTGGTGCT CCTCCGAGATATGCCAATGGCCGCCCTTCCCAATCGGGTAATAGGGTCTGCAGATATTTTGTTGCTGGAGAATGC CTCCGCGCGGACTGCCGTTTCAGTCATGACTTGGAGCGCGCCTTGTGTCGCTTTTGGTTGAGAGGTATGTGTGCT AAAAATGAAGCTTGCGAGTTTTTGCACCATTTGCCCAAGGATGTGGATGTGGCAAACTTGAACGTGGCGATGTCC CGAGCGAATATAGGACCACCCAGTCACTATGGTCCACCCGAGATGCACGCTCCCGTTGACGAGTTCCCTGTACTT GGGTATGAGGGAAATGTTATGAGGATGAATAAACCTGGAGGATATTATGATCCGGGACGAACACGATTCGCAGCT GCTGTCAAGAAACCAGCTCCTCCCAAGATTAATGGCGTTCAACTGGCTCGTGTGATGGGCCCGGCCGCGGATAAT TTGCATCACCAATCGGCTATTGTTGCTCCTCGACCGTCGCCACGTATAAAACTTCATCCACCTACCTTGGTACCG ACTTTACCTACCGGTGATGCTGTCAATCAGTTATACATGAATTATCGTCAACGTGCGTTACAGTTGGGAGCGGCA AGGAATGCTTGTTTGTCTCGTGCTGCTGATGCATGGAGACGAGGAGATGGCGCTGCTGCCAAGAGGTTTAGTAGG GAAGGACATGATCTGAATGCCAAAATGTCAACGGAGATGGCGAATGCGGCATCTAAATTAGTTAGGGAACGATCG AGTTTGGCGGAACAGGCTGTGAGGGCGAGAGATTTAAGTTGGTCGGATGATCCTGGGGACCGGAGTGCGAGGGGG AGACCTTGTGGGTCTGGTTTGGGTGTTGTTTTGGGTGTGGGGAGGAAGGATGTTGGAGATGGCAAGTTGACGACG GAGGAGAGGACGGAGTGTATGCTTGATTTACATGGTTTGCATTCGAATGAGGCTACGGAGGTGTTGGAACAATTT TTACTTTCGTTGGAAAGGGAACACTTTTATGGTTTAGCTTATGCGGTTGTTGGAGAAGAAAAGCATACTGGGACG CAAGATCCTTCTAGGGGCGCTTCGCGTAATAGATTGGCTACGGCTGTACGTGAATGGGTTCATCGTTGGGGATAT CCATGGAGCGAGAGAGATGGTATCATTTGCATTGATCCATTGACCCACGCGTCCGAGTAG
Gene	>AgabiH97|094790 ATGGCTACCCTCGCCGTCCCTTCCTCTTACACGCCCCTCTTTCCGACAGAGACACCACACGCACACGACTCTGCC ACCCTCAAGTTTCCCCACACAAGAGCTCAGCTCGCCGCCGTCGCGCGCCAACACAAACCAGCCGACACTCATGAT TCAGACACCGGCACAGACGACCTCGGCCGCGTTCCGTCTTCCTTTGTCGCAAAGGTCGTTTCTCTGCTCACAGAC GAAAAGGAAGACGAGCTCAAGGCGCTGCTCAAGGCCACCTACAGTCTAGACAATCAATCAGTCAGTATCTCCACG TAGGCCTAAACATTGTGTCCTGAACAGATATTCAACAGCTCGAAGAGTCTGTGCTGGACCTCATGCACAAGCACC GCGACGACGTCGCTGGCGTCCCATTTGTATTTTTGACGCCAACGCGTCGTCCCATTTCCCGTCCTTCTTCCCGGG CGTCATCTCACTCGGCCCGTCTCCACCCTGCACGCCCAGACACGCCCTCTTCCGCCCCACCTTCTCCCCTTGCCT GGTCACATCGTCGCCCTCACACCCCTGCCACCTCCCCACTCGCAAACGGCGAGCCAAGGTCATACATGTCCCCAA AGAGCGATTATTCTCCCTCATCGTCCCCTATCTTGGCCCACGCTCAAGCCACTCAAGCACAGGCCCAATGGACTG CCAGTCTTCCAGCCTCTCCTTTGCTCACCTCTCGTTCTCTCAATGCCAAAGCTTCAGAATTCAGACCCATCCCAA GGCCGCTTTCTTCAGCTTCTTCTCAACCTGGCTCACTTCTTCGTGCCGACACGCCTTCTCCAGACATGTGGGCTC ACAACTCCCCACGCGCCACCTCCAATCTCGCCATCGCCGCGCCACTCATAGCAGATCAGTCCACTTCCGCTCCAA GGGCACTCACCCCCAGCTCATCTTTGCGCTCCTTTGTCCGCCCCGATGAAGACGAAGACGAAGGCGAAGATCCCG ATCCCTATGATCCCTTCACTAACAATGGCCAGCTGCCAGCCCCGTTCCATTCCATTACCGTTTCCGACTTTGATA ATCAGTGGTCCGTATCACCGCCAGAAGAGTCAGAGCTCACTCATATGGCAACTCAACCTCCATACGAGTACGGCT ATTCGCCGTTCAACCCGAATGGAAACAATGATCCCATCGACGATGTTGAAGCCAACGCCGCTCTCACAGATGGCA TGACGCCCTTTGATGTTCTCAGTTCCGTCTTTGGCTCTACACTAGCCCCATCAGAGCTCGAAGAGGCTCTGGCCG CCAATGGATACGATTTCGAGCACGCGATGGCGTGGTTGATCGATCGTGGTTTACCTCAACAGCCAGCGTCGCCTA CACAAGTACGAATGCAGCCTATGGGCAATCGTGTCACTCTGGTATCACGCGATACCGGGGGAACCATACGTGGCA CTGTCAAAGGCGGATATCACAACAACAATAGCAATGGGACCGGTCGTGGTGCTCCTCCGAGATATGCCAATGGCC GCCCTTCCCAATCGGGTAATAGGGTCTGCAGATATTTTGTTGCTGGAGAATGCCTCCGCGCGGACTGCCGTTTCA GGTTGGTTTGCCGTCTCCATACTCTACATGTGCGCTGATGCATTTCTATATAGTCATGACTTGGAGCGCGCCTTG TGTCGCTTTTGGTTGAGAGGTATGTGTGCTAAAAATGAAGCTTGCGAGTTTTTGCACCATTTGCCCAAGGATGTG GATGTGGCAAACTTGAACGTGGCGATGTCCCGAGCGAATATAGGACCACCCAGTCACTATGGTCCACCCGAGATG CACGCTCCCGTTGACGAGTTCCCTGTACTTGGGTATGAGGGAAATGTTATGAGGATGAATAAACCTGGAGGATAT TATGATCCGGGACGAACACGATTCGCAGCTGCTGTCAAGAAACCAGCTCCTCCCAAGATTAATGGCGTTCAACTG GCTCGTGTGATGGGCCCGGCCGCGGATAATTTGCATCACCAATCGGCTATTGTTGCTCCTCGACCGTCGCCACGT ATAAAACTTCATCCACCTACCTTGGTACCGACTTTACCTACCGGTGATGCTGTCAATCAGTTATACATGAATTAT CGTCAACGTGCGTTACAGTTGGGAGCGGCAAGGAATGCTTGTTTGTCTCGTGCTGCTGATGCATGGAGACGAGGA GATGGCGCTGCTGCCAAGAGGTTTAGTAGGGAAGGACATGATCTGAATGCCAAAATGTCAACGGAGATGGCGAAT GCGGCATCTAAATTAGTTAGGGAACGATCGAGTTTGGCGGAACAGGCTGTGAGGGCGAGAGATTTAAGTTGGTCG GATGATCCTGGGGACCGGAGTGCGAGGGGGAGACCTTGTGGGTCTGGTTTGGGTGTTGTTTTGGGTGTGGGGAGG AAGGATGTTGGAGATGGCAAGTTGACGACGGAGGAGAGGACGGAGTGTATGCTTGATTTACATGGTTTGCATTCG AATGAGGCTACGGAGGTGTTGGAACAATTTTTACTTTCGGTGAGATTTCTATCATTATCAATTCGTTTCATGTGC GGGTGTTAATCATCTTGTAGTTGGAAAGGGAACACTTTTATGGTTTAGGTACGTTGTTACATGAACTGTTCAATT GAGAGACATGACACTGATGGATTTTGTTGCTAGCTTATGCGGTTGTTGGAGAAGAAAAGCATACTGGGACGCAAG ATCCTTCTAGGGGCGCTTCGCGTAATAGATTGGCTACGGCTGTACGTGAATGGGTTCATCGTTGGGGATATCCAT GGAGCGAGAGAGATGGTATCATTTGCATTGATCCATTGACCCACGCGTCCGAGTAG