© 2023 - Robin Ohm - Utrecht University - The Netherlands
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| Protein ID | AgabiH97|090060 |
| Gene name | |
| Location | scaffold_6:450361..451604 |
| Strand | - |
| Gene length (bp) | 1243 |
| Transcript length (bp) | 1104 |
| Coding sequence length (bp) | 1104 |
| Protein length (aa) | 368 |
| PFAM Domain ID | Short name | Long name | E-value | Start | End |
|---|---|---|---|---|---|
| PF09445 | Methyltransf_15 | RNA cap guanine-N2 methyltransferase | 1.0E-35 | 148 | 357 |
| PF13649 | Methyltransf_25 | Methyltransferase domain | 2.7E-07 | 150 | 204 |
| PF02475 | Met_10 | Met-10+ like-protein | 4.7E-07 | 136 | 209 |
| PF08241 | Methyltransf_11 | Methyltransferase domain | 2.1E-04 | 151 | 204 |
| Swissprot ID | Swissprot Description | Start | End | E-value |
|---|---|---|---|---|
| sp|Q96RS0|TGS1_HUMAN | Trimethylguanosine synthase OS=Homo sapiens GN=TGS1 PE=1 SV=3 | 96 | 359 | 9.0E-56 |
| sp|Q923W1|TGS1_MOUSE | Trimethylguanosine synthase OS=Mus musculus GN=Tgs1 PE=1 SV=2 | 96 | 359 | 2.0E-55 |
| sp|P85107|TGS1_RAT | Trimethylguanosine synthase OS=Rattus norvegicus GN=Tgs1 PE=1 SV=1 | 96 | 359 | 6.0E-54 |
| sp|Q09814|TGS1_SCHPO | Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tgs1 PE=1 SV=3 | 98 | 317 | 1.0E-33 |
| sp|Q12052|TGS1_YEAST | Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TGS1 PE=1 SV=1 | 102 | 317 | 3.0E-20 |
| GO Term | Description | Terminal node |
|---|---|---|
| GO:0008168 | methyltransferase activity | Yes |
| GO:0009452 | 7-methylguanosine RNA capping | Yes |
| GO:0001510 | RNA methylation | Yes |
| GO:0009451 | RNA modification | No |
| GO:0016070 | RNA metabolic process | No |
| GO:0006396 | RNA processing | No |
| GO:1901360 | organic cyclic compound metabolic process | No |
| GO:0071704 | organic substance metabolic process | No |
| GO:0008150 | biological_process | No |
| GO:0006139 | nucleobase-containing compound metabolic process | No |
| GO:0008152 | metabolic process | No |
| GO:0043412 | macromolecule modification | No |
| GO:0046483 | heterocycle metabolic process | No |
| GO:0090304 | nucleic acid metabolic process | No |
| GO:0043170 | macromolecule metabolic process | No |
| GO:0032259 | methylation | No |
| GO:0043414 | macromolecule methylation | No |
| GO:0006807 | nitrogen compound metabolic process | No |
| GO:0016740 | transferase activity | No |
| GO:0036260 | RNA capping | No |
| GO:0003674 | molecular_function | No |
| GO:0044238 | primary metabolic process | No |
| GO:0006725 | cellular aromatic compound metabolic process | No |
| GO:0009987 | cellular process | No |
| GO:0003824 | catalytic activity | No |
| GO:0044237 | cellular metabolic process | No |
| GO:0044260 | cellular macromolecule metabolic process | No |
| GO:0034641 | cellular nitrogen compound metabolic process | No |
| GO:0016741 | transferase activity, transferring one-carbon groups | No |
| Localizations | Signals | Cytoplasm | Nucleus | Extracellular | Cell membrane | Mitochondrion | Plastid | Endoplasmic reticulum | Lysosome vacuole | Golgi apparatus | Peroxisome |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Nucleus | Nuclear localization signal | 0.3351 | 0.9462 | 0.0212 | 0.0113 | 0.3084 | 0.0012 | 0.0397 | 0.0142 | 0.025 | 0.0147 |
Expression values
| Label | Description | Expression (RPKM) | Confidence interval (low) | Confidence interval (high) |
|---|---|---|---|---|
| Casing | Casing mycelium | 24.87 | 13.41 | 36.32 |
| Initials | Initials knots | 20.98 | 11.05 | 30.90 |
| Pileal_Stipeal_center | Stage I stipe center | 24.45 | 13.11 | 35.80 |
| Pileal_Stipeal_shell | Stage I stipe shell | 30.78 | 16.96 | 44.60 |
| DIF_stipe_center | Stage II stipe center | 25.44 | 13.76 | 37.12 |
| DIF_stipe_shell | Stage II stipe shell | 23.84 | 12.75 | 34.93 |
| DIF_stipe_skin | Stage II stipe skin | 25.36 | 13.69 | 37.03 |
| DIF_cap_skin | Stage II cap skin | 30.76 | 17.04 | 44.47 |
| DIF_cap_tissue | Stage II cap tissue | 28.43 | 15.40 | 41.46 |
| DIF_gill_tissue | Stage II gill tissue | 31.12 | 17.25 | 45.00 |
| YFB_stipe_center | Young fruiting body stipe center | 33.09 | 18.37 | 47.81 |
| YFB_stipe_shell | Young fruiting body stipe shell | 26.89 | 14.61 | 39.16 |
| YFB_stipe_skin | Young fruiting body stipe skin | 31.45 | 17.46 | 45.44 |
| YFB_cap_skin | Young fruiting body cap skin | 28.58 | 15.68 | 41.48 |
| YFB_cap_tissue | Young fruiting body cap tissue | 19.60 | 10.24 | 28.97 |
| YFB_gill_tissue | Young fruiting body gill tissue | 30.51 | 16.89 | 44.13 |
| YFB_veil | Young fruiting body veil | 30.44 | 16.83 | 44.05 |
Differential expression
| Label1 | Label2 | Q-value | Significant difference |
|---|---|---|---|
| Casing | DIF_gill_tissue | 0.594698 | no |
| Casing | YFB_stipe_center | 0.454694 | no |
| Casing | YFB_stipe_shell | 0.890815 | no |
| Casing | YFB_stipe_skin | 0.573594 | no |
| Casing | YFB_cap_skin | 0.775726 | no |
| Casing | YFB_cap_tissue | 0.577809 | no |
| Casing | YFB_gill_tissue | 0.639833 | no |
| Casing | YFB_veil | 0.642998 | no |
| Casing | Initials | 0.727344 | no |
| Casing | Pileal_Stipeal_center | 0.978181 | no |
| Casing | Pileal_Stipeal_shell | 0.612095 | no |
| Casing | DIF_stipe_center | 0.970864 | no |
| Casing | DIF_stipe_shell | 0.944038 | no |
| Casing | DIF_stipe_skin | 0.974287 | no |
| Casing | DIF_cap_skin | 0.619572 | no |
| Casing | DIF_cap_tissue | 0.792677 | no |
| DIF_gill_tissue | YFB_stipe_center | 0.914359 | no |
| DIF_gill_tissue | YFB_stipe_shell | 0.762880 | no |
| DIF_gill_tissue | YFB_stipe_skin | 0.986598 | no |
| DIF_gill_tissue | YFB_cap_skin | 0.874839 | no |
| DIF_gill_tissue | YFB_cap_tissue | 0.160121 | no |
| DIF_gill_tissue | YFB_gill_tissue | 0.974385 | no |
| DIF_gill_tissue | YFB_veil | 0.972375 | no |
| YFB_stipe_center | YFB_stipe_shell | 0.636432 | no |
| YFB_stipe_center | YFB_stipe_skin | 0.932040 | no |
| YFB_stipe_center | YFB_cap_skin | 0.759155 | no |
| YFB_stipe_center | YFB_cap_tissue | 0.101542 | no |
| YFB_stipe_center | YFB_gill_tissue | 0.884352 | no |
| YFB_stipe_center | YFB_veil | 0.881719 | no |
| YFB_stipe_shell | YFB_stipe_skin | 0.745775 | no |
| YFB_stipe_shell | YFB_cap_skin | 0.917482 | no |
| YFB_stipe_shell | YFB_cap_tissue | 0.414173 | no |
| YFB_stipe_shell | YFB_gill_tissue | 0.804203 | no |
| YFB_stipe_shell | YFB_veil | 0.808900 | no |
| YFB_stipe_skin | YFB_cap_skin | 0.854683 | no |
| YFB_stipe_skin | YFB_cap_tissue | 0.147277 | no |
| YFB_stipe_skin | YFB_gill_tissue | 0.959769 | no |
| YFB_stipe_skin | YFB_veil | 0.958192 | no |
| YFB_cap_skin | YFB_cap_tissue | 0.292985 | no |
| YFB_cap_skin | YFB_gill_tissue | 0.908698 | no |
| YFB_cap_skin | YFB_veil | 0.912764 | no |
| YFB_cap_tissue | YFB_gill_tissue | 0.188653 | no |
| YFB_cap_tissue | YFB_veil | 0.189886 | no |
| YFB_gill_tissue | YFB_veil | 0.996674 | no |
| Initials | DIF_gill_tissue | 0.258617 | no |
| Initials | YFB_stipe_center | 0.172056 | no |
| Initials | YFB_stipe_shell | 0.554291 | no |
| Initials | YFB_stipe_skin | 0.243209 | no |
| Initials | YFB_cap_skin | 0.416825 | no |
| Initials | YFB_cap_tissue | 0.912495 | no |
| Initials | YFB_gill_tissue | 0.292131 | no |
| Initials | YFB_veil | 0.295530 | no |
| Initials | Pileal_Stipeal_center | 0.756560 | no |
| Initials | Pileal_Stipeal_shell | 0.271154 | no |
| Initials | DIF_stipe_center | 0.676018 | no |
| Initials | DIF_stipe_shell | 0.809147 | no |
| Initials | DIF_stipe_skin | 0.678558 | no |
| Initials | DIF_cap_skin | 0.276718 | no |
| Initials | DIF_cap_tissue | 0.446272 | no |
| Pileal_Stipeal_center | DIF_gill_tissue | 0.549543 | no |
| Pileal_Stipeal_center | YFB_stipe_center | 0.418972 | no |
| Pileal_Stipeal_center | YFB_stipe_shell | 0.860717 | no |
| Pileal_Stipeal_center | YFB_stipe_skin | 0.530001 | no |
| Pileal_Stipeal_center | YFB_cap_skin | 0.740828 | no |
| Pileal_Stipeal_center | YFB_cap_tissue | 0.610881 | no |
| Pileal_Stipeal_center | YFB_gill_tissue | 0.596755 | no |
| Pileal_Stipeal_center | YFB_veil | 0.600155 | no |
| Pileal_Stipeal_center | Pileal_Stipeal_shell | 0.571264 | no |
| Pileal_Stipeal_center | DIF_stipe_center | 0.948803 | no |
| Pileal_Stipeal_center | DIF_stipe_shell | 0.965841 | no |
| Pileal_Stipeal_center | DIF_stipe_skin | 0.952047 | no |
| Pileal_Stipeal_center | DIF_cap_skin | 0.576613 | no |
| Pileal_Stipeal_center | DIF_cap_tissue | 0.759610 | no |
| Pileal_Stipeal_shell | DIF_gill_tissue | 0.985119 | no |
| Pileal_Stipeal_shell | YFB_stipe_center | 0.897539 | no |
| Pileal_Stipeal_shell | YFB_stipe_shell | 0.790008 | no |
| Pileal_Stipeal_shell | YFB_stipe_skin | 0.972996 | no |
| Pileal_Stipeal_shell | YFB_cap_skin | 0.894478 | no |
| Pileal_Stipeal_shell | YFB_cap_tissue | 0.170751 | no |
| Pileal_Stipeal_shell | YFB_gill_tissue | 0.988451 | no |
| Pileal_Stipeal_shell | YFB_veil | 0.985568 | no |
| Pileal_Stipeal_shell | DIF_stipe_center | 0.669500 | no |
| Pileal_Stipeal_shell | DIF_stipe_shell | 0.521971 | no |
| Pileal_Stipeal_shell | DIF_stipe_skin | 0.659480 | no |
| Pileal_Stipeal_shell | DIF_cap_skin | 0.998426 | no |
| Pileal_Stipeal_shell | DIF_cap_tissue | 0.888742 | no |
| DIF_stipe_center | DIF_gill_tissue | 0.648298 | no |
| DIF_stipe_center | YFB_stipe_center | 0.505497 | no |
| DIF_stipe_center | YFB_stipe_shell | 0.925892 | no |
| DIF_stipe_center | YFB_stipe_skin | 0.624647 | no |
| DIF_stipe_center | YFB_cap_skin | 0.819899 | no |
| DIF_stipe_center | YFB_cap_tissue | 0.528877 | no |
| DIF_stipe_center | YFB_gill_tissue | 0.689007 | no |
| DIF_stipe_center | YFB_veil | 0.693368 | no |
| DIF_stipe_center | DIF_stipe_shell | 0.912356 | no |
| DIF_stipe_center | DIF_stipe_skin | 0.995232 | no |
| DIF_stipe_center | DIF_cap_skin | 0.671979 | no |
| DIF_stipe_center | DIF_cap_tissue | 0.836849 | no |
| DIF_stipe_shell | DIF_gill_tissue | 0.494559 | no |
| DIF_stipe_shell | YFB_stipe_center | 0.362644 | no |
| DIF_stipe_shell | YFB_stipe_shell | 0.816286 | no |
| DIF_stipe_shell | YFB_stipe_skin | 0.475834 | no |
| DIF_stipe_shell | YFB_cap_skin | 0.684468 | no |
| DIF_stipe_shell | YFB_cap_tissue | 0.665751 | no |
| DIF_stipe_shell | YFB_gill_tissue | 0.536942 | no |
| DIF_stipe_shell | YFB_veil | 0.542795 | no |
| DIF_stipe_shell | DIF_stipe_skin | 0.914572 | no |
| DIF_stipe_shell | DIF_cap_skin | 0.519162 | no |
| DIF_stipe_shell | DIF_cap_tissue | 0.704331 | no |
| DIF_stipe_skin | DIF_gill_tissue | 0.640450 | no |
| DIF_stipe_skin | YFB_stipe_center | 0.500103 | no |
| DIF_stipe_skin | YFB_stipe_shell | 0.921415 | no |
| DIF_stipe_skin | YFB_stipe_skin | 0.617888 | no |
| DIF_stipe_skin | YFB_cap_skin | 0.814343 | no |
| DIF_stipe_skin | YFB_cap_tissue | 0.536380 | no |
| DIF_stipe_skin | YFB_gill_tissue | 0.681587 | no |
| DIF_stipe_skin | YFB_veil | 0.686330 | no |
| DIF_stipe_skin | DIF_cap_skin | 0.664782 | no |
| DIF_stipe_skin | DIF_cap_tissue | 0.831718 | no |
| DIF_cap_skin | DIF_gill_tissue | 0.983711 | no |
| DIF_cap_skin | YFB_stipe_center | 0.895917 | no |
| DIF_cap_skin | YFB_stipe_shell | 0.787449 | no |
| DIF_cap_skin | YFB_stipe_skin | 0.971168 | no |
| DIF_cap_skin | YFB_cap_skin | 0.895973 | no |
| DIF_cap_skin | YFB_cap_tissue | 0.172056 | no |
| DIF_cap_skin | YFB_gill_tissue | 0.989064 | no |
| DIF_cap_skin | YFB_veil | 0.986025 | no |
| DIF_cap_skin | DIF_cap_tissue | 0.889398 | no |
| DIF_cap_tissue | DIF_gill_tissue | 0.871735 | no |
| DIF_cap_tissue | YFB_stipe_center | 0.753645 | no |
| DIF_cap_tissue | YFB_stipe_shell | 0.927079 | no |
| DIF_cap_tissue | YFB_stipe_skin | 0.855184 | no |
| DIF_cap_tissue | YFB_cap_skin | 0.992590 | no |
| DIF_cap_tissue | YFB_cap_tissue | 0.309634 | no |
| DIF_cap_tissue | YFB_gill_tissue | 0.904295 | no |
| DIF_cap_tissue | YFB_veil | 0.908837 | no |
| Orthofinder run ID | 1 |
| Orthogroup | 5214 |
| Change Orthofinder run |
| Species | Protein ID |
|---|---|
| Agaricus bisporus var bisporus H39 | AgabiH39|375200 |
| Agaricus bisporus var bisporus H97 | AgabiH97|090060 (this protein) |
| Rhodonia placenta FPRL280 | RhoplFPRL280|20_48 |
| Type of sequence | Sequence |
|---|---|
| Locus | Download genbank file of locus
Download genbank file of locus (reverse complement)
The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded. |
| Protein | >AgabiH97|090060 MGKRKSRTGMTGLSRFISALNEGESGTQPSISTNSVDPAVSAHEQNDLTMKEVNGETNTDSASAPIFTSVATISK KQKIIPHYDATGLVPTYTNEAMVPDHLKKYFSQRTRYFSLYDTPPGCLLDEEGWYSVTPERVANQIAERCRCDVV LDAFCGVGGNTIAFAKTCERVIALDTNPTRLALARHNAQIYGVADRIEFIQADYISFARAYLSSFSTPSPSSNTS TPPRTKTRKIDVVFLSPPWGGPSYLSGSPSHDIVNNVAKDNLPQTDGQDSHPTYSFSSIQPIHGKDLFTLTRRIT SNIAYYLPRNTDLPEISDLLNATDDHGRSVKLSGERIEVEEEWMGSKLKALTCYFGGLVAGQEDLFS* |
| Coding | >AgabiH97|090060 ATGGGCAAACGTAAATCAAGGACTGGGATGACTGGGCTGTCGCGTTTTATCAGTGCTTTGAATGAAGGAGAGTCC GGGACACAACCCTCGATCTCAACGAACTCTGTCGACCCAGCTGTCTCGGCTCACGAGCAGAACGATCTTACAATG AAAGAAGTTAATGGCGAAACAAACACAGACTCTGCGTCAGCGCCAATCTTTACTTCTGTCGCAACAATATCGAAG AAACAAAAAATCATTCCTCACTATGACGCGACCGGTCTCGTACCCACATATACCAACGAAGCTATGGTCCCAGAT CATCTCAAGAAATACTTCTCTCAGCGAACACGATATTTTTCGTTATACGATACGCCGCCTGGATGCCTTCTCGAT GAAGAAGGATGGTACAGCGTGACACCTGAGAGGGTTGCAAATCAAATAGCTGAGCGATGTCGATGCGACGTCGTT TTAGATGCTTTCTGCGGTGTCGGTGGGAATACTATCGCTTTTGCCAAAACTTGCGAACGAGTGATTGCGCTGGAT ACCAATCCCACGAGGTTAGCGCTGGCGAGGCACAACGCTCAGATATACGGCGTTGCAGATCGTATAGAATTTATC CAAGCAGATTATATTTCATTTGCCCGCGCATATCTTTCCTCGTTCTCGACCCCGTCGCCGTCATCAAATACCTCG ACGCCGCCACGAACTAAAACAAGAAAGATTGACGTCGTGTTTCTCAGTCCTCCGTGGGGTGGCCCTTCCTACCTC TCAGGTTCACCCAGCCATGACATCGTCAACAACGTTGCCAAGGATAATCTCCCTCAGACTGATGGTCAAGATTCA CACCCCACTTATTCCTTTTCATCTATTCAGCCTATTCACGGGAAAGATCTTTTTACTCTCACACGCAGAATCACA TCAAACATTGCATATTATTTACCTCGCAACACTGATCTTCCAGAAATCAGTGATTTGTTAAATGCTACCGATGAT CATGGCCGTTCGGTAAAGTTATCGGGAGAGCGGATTGAGGTTGAAGAGGAGTGGATGGGGAGTAAGCTGAAAGCT TTGACGTGTTATTTTGGTGGGCTTGTCGCCGGACAGGAAGATTTGTTCTCTTAG |
| Transcript | >AgabiH97|090060 ATGGGCAAACGTAAATCAAGGACTGGGATGACTGGGCTGTCGCGTTTTATCAGTGCTTTGAATGAAGGAGAGTCC GGGACACAACCCTCGATCTCAACGAACTCTGTCGACCCAGCTGTCTCGGCTCACGAGCAGAACGATCTTACAATG AAAGAAGTTAATGGCGAAACAAACACAGACTCTGCGTCAGCGCCAATCTTTACTTCTGTCGCAACAATATCGAAG AAACAAAAAATCATTCCTCACTATGACGCGACCGGTCTCGTACCCACATATACCAACGAAGCTATGGTCCCAGAT CATCTCAAGAAATACTTCTCTCAGCGAACACGATATTTTTCGTTATACGATACGCCGCCTGGATGCCTTCTCGAT GAAGAAGGATGGTACAGCGTGACACCTGAGAGGGTTGCAAATCAAATAGCTGAGCGATGTCGATGCGACGTCGTT TTAGATGCTTTCTGCGGTGTCGGTGGGAATACTATCGCTTTTGCCAAAACTTGCGAACGAGTGATTGCGCTGGAT ACCAATCCCACGAGGTTAGCGCTGGCGAGGCACAACGCTCAGATATACGGCGTTGCAGATCGTATAGAATTTATC CAAGCAGATTATATTTCATTTGCCCGCGCATATCTTTCCTCGTTCTCGACCCCGTCGCCGTCATCAAATACCTCG ACGCCGCCACGAACTAAAACAAGAAAGATTGACGTCGTGTTTCTCAGTCCTCCGTGGGGTGGCCCTTCCTACCTC TCAGGTTCACCCAGCCATGACATCGTCAACAACGTTGCCAAGGATAATCTCCCTCAGACTGATGGTCAAGATTCA CACCCCACTTATTCCTTTTCATCTATTCAGCCTATTCACGGGAAAGATCTTTTTACTCTCACACGCAGAATCACA TCAAACATTGCATATTATTTACCTCGCAACACTGATCTTCCAGAAATCAGTGATTTGTTAAATGCTACCGATGAT CATGGCCGTTCGGTAAAGTTATCGGGAGAGCGGATTGAGGTTGAAGAGGAGTGGATGGGGAGTAAGCTGAAAGCT TTGACGTGTTATTTTGGTGGGCTTGTCGCCGGACAGGAAGATTTGTTCTCTTAG |
| Gene | >AgabiH97|090060 ATGGGCAAACGTAAATCAAGGACTGGGATGACTGGGCTGTCGCGTTTTATCAGTGCTTTGAATGAAGGAGAGTCC GGGACACAACCCTCGATCTCAACGAACTCTGTCGACCCAGCTGTCTCGGCTCACGAGCAGAACGATCTTACAATG AAAGAAGTTAATGGCGAAACAAACACAGACTCTGCGTCAGCGCCAATCTTTACTTCTGTCGCAACAATATCGAAG AAACAAAAAATCATTCCTCACTATGACGCGACCGGTCTCGTACCCACATATACCAACGAAGCTATGGTCCCAGAT CATCTCAAGAAATGTCCGTGCTTGTCATAGGGTTCTTAACGTCGCTTAAAACGACAAACTCATGCCACTTGTATT GGTTGCCTCCGCTTGTAGACTTCTCTCAGCGAACACGATATTTTTCGTTATACGATACGCCGCCTGGATGCCTTC TCGATGAAGAAGGATGGTACAGCGTGACACCTGAGAGGGTTGCAAATCAAATAGCTGAGCGATGTCGATGCGACG TCGTTTTAGATGCTTTCTGCGGTGTCGGTGGGAATACTATCGCTTTTGCCAAAACTTGCGAACGAGGTTTGGAGG ACAAATTTATCTTTTTTTTTTACCTTCAGCTGAGGCTCGCCCTTTTCTAGTGATTGCGCTGGATACCAATCCCAC GAGGTTAGCGCTGGCGAGGCACAACGCTCAGATATACGGCGTTGCAGATCGTATAGAATTTATCCAAGCAGATTA TATTTCATTTGCCCGCGCATATCTTTCCTCGTTCTCGACCCCGTCGCCGTCATCAAATACCTCGACGCCGCCACG AACTAAAACAAGAAAGATTGACGTCGTGTTTCTCAGTCCTCCGTGGGGTGGCCCTTCCTACCTCTCAGGTTCACC CAGCCATGACATCGTCAACAACGTTGCCAAGGATAATCTCCCTCAGACTGATGGTCAAGATTCACACCCCACTTA TTCCTTTTCATCTATTCAGCCTATTCACGGGAAAGATCTTTTTACTCTCACACGCAGAATCACATCAAACATTGC ATATTATTTACCTCGCAACACTGATCTTCCAGAAATCAGTGATTTGTTAAATGCTACCGATGATCATGGCCGTTC GGTAAAGTTATCGGGAGAGCGGATTGAGGTTGAAGAGGAGTGGATGGGGAGTAAGCTGAAAGCTTTGACGTGTTA TTTTGGTGGGCTTGTCGCCGGACAGGAAGATTTGTTCTCTTAG |