Fungal Genomics

at Utrecht University

General Properties

Protein ID	AgabiH97\|040190
Gene name
Location	scaffold_2:450852..454485
Strand	-
Gene length (bp)	3633
Transcript length (bp)	2571
Coding sequence length (bp)	2571
Protein length (aa)	857

PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF04100	Vps53_N	Vps53-like, N-terminal	3.0E-88	26	387
PF16854	VPS53_C	Vacuolar protein sorting-associated protein 53 C-terminus	3.1E-06	634	718

Swissprot hits

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Swissprot ID	Swissprot Description	Start	End	E-value
sp\|Q5ZLD7\|VPS53_CHICK	Vacuolar protein sorting-associated protein 53 homolog OS=Gallus gallus GN=VPS53 PE=2 SV=1	1	717	6.0E-114
sp\|Q8CCB4\|VPS53_MOUSE	Vacuolar protein sorting-associated protein 53 homolog OS=Mus musculus GN=Vps53 PE=1 SV=1	9	765	7.0E-113
sp\|Q5R5J4\|VPS53_PONAB	Vacuolar protein sorting-associated protein 53 homolog OS=Pongo abelii GN=VPS53 PE=2 SV=1	6	637	9.0E-94
sp\|Q5VIR6\|VPS53_HUMAN	Vacuolar protein sorting-associated protein 53 homolog OS=Homo sapiens GN=VPS53 PE=1 SV=1	6	637	1.0E-93
sp\|P87129\|VPS53_SCHPO	Vacuolar protein sorting-associated protein 53 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vps53 PE=1 SV=1	24	717	3.0E-83

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Swissprot ID	Swissprot Description	Start	End	E-value
sp\|Q5ZLD7\|VPS53_CHICK	Vacuolar protein sorting-associated protein 53 homolog OS=Gallus gallus GN=VPS53 PE=2 SV=1	1	717	6.0E-114
sp\|Q8CCB4\|VPS53_MOUSE	Vacuolar protein sorting-associated protein 53 homolog OS=Mus musculus GN=Vps53 PE=1 SV=1	9	765	7.0E-113
sp\|Q5R5J4\|VPS53_PONAB	Vacuolar protein sorting-associated protein 53 homolog OS=Pongo abelii GN=VPS53 PE=2 SV=1	6	637	9.0E-94
sp\|Q5VIR6\|VPS53_HUMAN	Vacuolar protein sorting-associated protein 53 homolog OS=Homo sapiens GN=VPS53 PE=1 SV=1	6	637	1.0E-93
sp\|P87129\|VPS53_SCHPO	Vacuolar protein sorting-associated protein 53 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vps53 PE=1 SV=1	24	717	3.0E-83
sp\|Q0WQF4\|VP53A_ARATH	Vacuolar protein sorting-associated protein 53 A OS=Arabidopsis thaliana GN=VPS53 PE=1 SV=1	28	726	6.0E-83
sp\|P34561\|VPS53_CAEEL	Vacuolar protein sorting-associated protein 53 homolog OS=Caenorhabditis elegans GN=vps-53 PE=1 SV=4	33	719	4.0E-71
sp\|P47061\|VPS53_YEAST	Vacuolar protein sorting-associated protein 53 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VPS53 PE=1 SV=1	26	718	5.0E-46
sp\|F4I7Y2\|VP53B_ARATH	Vacuolar protein sorting-associated protein 53 B OS=Arabidopsis thaliana GN=At1g50970 PE=2 SV=1	30	387	4.0E-33

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GO

(None)

SignalP

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SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
No	1 - 12	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Developmental stages of Agaricus bisporus (strain A15). Published in Pelkmans et al, Applied Microbiology and Biotechnology, 2016

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Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>AgabiH97\|040190 MQTDDLPHEIVLAIERALEPAPENQEFNPLTIINELFPNEASLAYIDSVDRKLNETQSKFQKEIDELQEELKKNQ DPERMSMIQEMISDLLGQMSRIREKATESEAVVRNITKDIQVLDLAKKNLILSMTVLRRLQMLVNAMTQLQDYIK EKRYKEVSESLATVKEISATFKQYTSVQRISRVWKRIQELQGELRTQLDADFDAFFIQDSTKQVKPALITQACLV VDVLGSDFRTHLIDRHVALELKEYRRIFKTNDEAGQLDNISRRFAWFRRLIQNHELEQGRVFPSLWRVGWYLLAK FAEITRDDISALLSKAASNMTVKTLLDYLQQTIEFELSMAKKWATPFAEILKFTNGSSQPQKVISSSFEPHLGIF VDAQDKVLAEMLAPHRKKGKPPPRTSLETNRASTEENADDEPPIVVLPSSTELFYLYGQSLEQCAKLSTGKTLFD LCNLHKKWLRIYAEDVLSLSNPKQRPTARRSMESRFDINEIKRACLVINTADYCQTTASELEEKIKEKINEDFKE KITLQKERDLFVSVISSAITLLLREVENATDVYFTTMIRSNWPALNQVSGQSHYVGELVKATEQVVETIKPLIEQ KRYLRNFLDKVCSVVLAKFTNALVRSKPLKEIGAEQLLLDLQVLKGYLTKMPGENLLTNIYSKALTKTATRLETL LKVIITPMDPKENFILNYTFLIGDASLINFQKILDLKGVPRTLHQDFEDTFLMITSTKTDLDSTSFLSSLDMDPQ GGGGGGSNLLLLSPTASRVALPLGSNTISDGGGLFHSLSGGSGGASVSGSSGPATGSSVGGGVGGVGTGEGGFGP GARGMSGETNVFSDFRRFVSFGLRKESTPPS*
Coding	>AgabiH97\|040190 ATGCAAACCGATGATTTGCCTCACGAAATAGTCCTCGCCATTGAGCGCGCGCTCGAGCCTGCTCCGGAAAACCAA GAATTCAATCCTCTCACAATCATCAATGAGCTGTTTCCTAATGAGGCTTCACTCGCCTATATTGACTCTGTGGAT CGGAAACTTAATGAGACACAATCCAAATTTCAGAAAGAGATAGATGAACTTCAAGAGGAATTGAAAAAGAACCAG GATCCCGAGCGTATGTCCATGATTCAAGAGATGATTTCCGACCTCTTGGGACAAATGTCACGTATTCGTGAAAAA GCTACTGAATCGGAAGCAGTTGTCAGGAATATTACGAAGGACATACAGGTCCTGGATCTCGCGAAGAAGAATTTG ATCCTTAGTATGACTGTATTAAGACGGTTGCAGATGCTAGTCAATGCAATGACGCAGCTCCAGGATTACATCAAG GAAAAGAGGTACAAAGAAGTATCCGAATCCCTAGCCACTGTCAAAGAAATATCCGCTACTTTCAAACAATACACA TCAGTTCAACGAATCTCAAGAGTCTGGAAACGAATACAAGAATTGCAAGGGGAGTTACGCACTCAGTTAGATGCA GACTTCGACGCCTTCTTTATTCAGGATTCCACGAAACAAGTCAAACCCGCTTTGATAACTCAAGCTTGTCTCGTC GTCGACGTTCTAGGTTCTGATTTTCGTACCCACCTCATAGACCGTCACGTTGCACTCGAACTCAAAGAATACCGA CGTATATTCAAAACCAACGATGAAGCTGGCCAGTTGGATAATATCTCGAGACGTTTCGCTTGGTTCAGGCGGTTG ATACAGAACCATGAGCTTGAACAGGGTAGGGTGTTTCCATCGCTTTGGAGAGTTGGGTGGTACTTGTTAGCCAAA TTCGCCGAGATCACAAGGGATGATATTTCGGCGTTGCTGTCAAAGGCGGCATCGAATATGACTGTGAAAACGTTA CTCGATTATCTACAACAGACCATAGAGTTCGAGCTTTCAATGGCGAAGAAATGGGCAACCCCCTTTGCGGAAATA TTGAAGTTTACCAACGGCTCCAGTCAGCCGCAGAAAGTTATATCCTCATCCTTTGAGCCTCATCTTGGCATATTC GTCGATGCTCAAGACAAAGTTTTGGCAGAGATGCTGGCACCACATCGAAAGAAGGGAAAACCTCCACCGAGGACG TCCCTTGAAACTAACCGTGCATCGACTGAAGAAAATGCAGATGACGAACCACCAATAGTTGTCTTACCCTCATCT ACAGAATTGTTTTATCTTTATGGACAGAGTTTGGAGCAATGTGCGAAATTATCGACTGGGAAAACATTGTTTGAC CTATGTAATCTGCACAAGAAGTGGTTGAGAATATATGCAGAGGATGTCTTGTCATTGAGCAATCCCAAACAACGT CCCACGGCGCGTAGATCCATGGAATCGCGATTTGACATAAACGAAATTAAACGAGCGTGTTTGGTCATCAATACC GCGGACTATTGCCAAACTACAGCGTCCGAGCTCGAAGAGAAAATCAAGGAAAAGATTAATGAAGATTTCAAAGAG AAAATCACACTGCAAAAGGAACGTGATCTCTTTGTCAGTGTCATATCATCAGCTATAACTCTCCTCCTTCGCGAA GTTGAGAACGCCACTGATGTATATTTTACGACAATGATCCGTTCGAATTGGCCAGCTTTGAACCAAGTCAGCGGG CAGTCACATTATGTTGGGGAATTGGTCAAGGCTACGGAACAGGTGGTGGAGACTATCAAACCGTTGATCGAGCAA AAAAGATATCTGAGGAATTTTCTGGATAAAGTTTGTAGTGTCGTGTTGGCCAAATTTACAAATGCGCTTGTGAGA AGTAAACCATTAAAAGAGATTGGTGCCGAACAGCTCCTACTTGACTTACAAGTTCTCAAAGGGTATCTTACTAAG ATGCCGGGAGAGAATTTGTTAACGAATATTTACTCGAAGGCGTTAACCAAAACGGCGACACGACTTGAGACGCTT CTTAAAGTCATCATAACTCCAATGGACCCAAAAGAGAACTTTATACTCAACTATACATTCCTCATCGGAGATGCT TCACTAATCAACTTCCAAAAGATTCTTGACCTAAAAGGAGTCCCGCGCACTCTTCATCAAGATTTCGAAGACACA TTCTTGATGATCACGAGTACCAAGACCGATTTAGACAGTACTTCTTTCTTATCTTCGCTCGATATGGATCCCCAA GGAGGTGGAGGAGGAGGAAGCAATCTTCTCCTTCTCAGTCCTACAGCTAGTCGAGTTGCATTACCGCTTGGATCA AATACCATTAGCGATGGTGGTGGTTTATTTCATAGTTTAAGCGGCGGGAGTGGTGGTGCGTCGGTATCCGGTTCG AGTGGACCAGCGACGGGTTCGAGTGTTGGTGGTGGGGTGGGTGGTGTTGGTACTGGCGAAGGCGGTTTTGGTCCT GGAGCGAGAGGAATGTCGGGTGAGACGAATGTCTTTTCTGATTTTAGAAGATTTGTTAGTTTTGGATTGAGGAAA GAGTCGACTCCGCCTTCATAA
Transcript	>AgabiH97\|040190 ATGCAAACCGATGATTTGCCTCACGAAATAGTCCTCGCCATTGAGCGCGCGCTCGAGCCTGCTCCGGAAAACCAA GAATTCAATCCTCTCACAATCATCAATGAGCTGTTTCCTAATGAGGCTTCACTCGCCTATATTGACTCTGTGGAT CGGAAACTTAATGAGACACAATCCAAATTTCAGAAAGAGATAGATGAACTTCAAGAGGAATTGAAAAAGAACCAG GATCCCGAGCGTATGTCCATGATTCAAGAGATGATTTCCGACCTCTTGGGACAAATGTCACGTATTCGTGAAAAA GCTACTGAATCGGAAGCAGTTGTCAGGAATATTACGAAGGACATACAGGTCCTGGATCTCGCGAAGAAGAATTTG ATCCTTAGTATGACTGTATTAAGACGGTTGCAGATGCTAGTCAATGCAATGACGCAGCTCCAGGATTACATCAAG GAAAAGAGGTACAAAGAAGTATCCGAATCCCTAGCCACTGTCAAAGAAATATCCGCTACTTTCAAACAATACACA TCAGTTCAACGAATCTCAAGAGTCTGGAAACGAATACAAGAATTGCAAGGGGAGTTACGCACTCAGTTAGATGCA GACTTCGACGCCTTCTTTATTCAGGATTCCACGAAACAAGTCAAACCCGCTTTGATAACTCAAGCTTGTCTCGTC GTCGACGTTCTAGGTTCTGATTTTCGTACCCACCTCATAGACCGTCACGTTGCACTCGAACTCAAAGAATACCGA CGTATATTCAAAACCAACGATGAAGCTGGCCAGTTGGATAATATCTCGAGACGTTTCGCTTGGTTCAGGCGGTTG ATACAGAACCATGAGCTTGAACAGGGTAGGGTGTTTCCATCGCTTTGGAGAGTTGGGTGGTACTTGTTAGCCAAA TTCGCCGAGATCACAAGGGATGATATTTCGGCGTTGCTGTCAAAGGCGGCATCGAATATGACTGTGAAAACGTTA CTCGATTATCTACAACAGACCATAGAGTTCGAGCTTTCAATGGCGAAGAAATGGGCAACCCCCTTTGCGGAAATA TTGAAGTTTACCAACGGCTCCAGTCAGCCGCAGAAAGTTATATCCTCATCCTTTGAGCCTCATCTTGGCATATTC GTCGATGCTCAAGACAAAGTTTTGGCAGAGATGCTGGCACCACATCGAAAGAAGGGAAAACCTCCACCGAGGACG TCCCTTGAAACTAACCGTGCATCGACTGAAGAAAATGCAGATGACGAACCACCAATAGTTGTCTTACCCTCATCT ACAGAATTGTTTTATCTTTATGGACAGAGTTTGGAGCAATGTGCGAAATTATCGACTGGGAAAACATTGTTTGAC CTATGTAATCTGCACAAGAAGTGGTTGAGAATATATGCAGAGGATGTCTTGTCATTGAGCAATCCCAAACAACGT CCCACGGCGCGTAGATCCATGGAATCGCGATTTGACATAAACGAAATTAAACGAGCGTGTTTGGTCATCAATACC GCGGACTATTGCCAAACTACAGCGTCCGAGCTCGAAGAGAAAATCAAGGAAAAGATTAATGAAGATTTCAAAGAG AAAATCACACTGCAAAAGGAACGTGATCTCTTTGTCAGTGTCATATCATCAGCTATAACTCTCCTCCTTCGCGAA GTTGAGAACGCCACTGATGTATATTTTACGACAATGATCCGTTCGAATTGGCCAGCTTTGAACCAAGTCAGCGGG CAGTCACATTATGTTGGGGAATTGGTCAAGGCTACGGAACAGGTGGTGGAGACTATCAAACCGTTGATCGAGCAA AAAAGATATCTGAGGAATTTTCTGGATAAAGTTTGTAGTGTCGTGTTGGCCAAATTTACAAATGCGCTTGTGAGA AGTAAACCATTAAAAGAGATTGGTGCCGAACAGCTCCTACTTGACTTACAAGTTCTCAAAGGGTATCTTACTAAG ATGCCGGGAGAGAATTTGTTAACGAATATTTACTCGAAGGCGTTAACCAAAACGGCGACACGACTTGAGACGCTT CTTAAAGTCATCATAACTCCAATGGACCCAAAAGAGAACTTTATACTCAACTATACATTCCTCATCGGAGATGCT TCACTAATCAACTTCCAAAAGATTCTTGACCTAAAAGGAGTCCCGCGCACTCTTCATCAAGATTTCGAAGACACA TTCTTGATGATCACGAGTACCAAGACCGATTTAGACAGTACTTCTTTCTTATCTTCGCTCGATATGGATCCCCAA GGAGGTGGAGGAGGAGGAAGCAATCTTCTCCTTCTCAGTCCTACAGCTAGTCGAGTTGCATTACCGCTTGGATCA AATACCATTAGCGATGGTGGTGGTTTATTTCATAGTTTAAGCGGCGGGAGTGGTGGTGCGTCGGTATCCGGTTCG AGTGGACCAGCGACGGGTTCGAGTGTTGGTGGTGGGGTGGGTGGTGTTGGTACTGGCGAAGGCGGTTTTGGTCCT GGAGCGAGAGGAATGTCGGGTGAGACGAATGTCTTTTCTGATTTTAGAAGATTTGTTAGTTTTGGATTGAGGAAA GAGTCGACTCCGCCTTCATAA
Gene	>AgabiH97\|040190 ATGCAAACCGATGATTTGCCTCACGAAATAGTCCTCGCCATTGAGCGCGCGCTCGAGCCTGCTCCGGAAAACCAA GAATTCAATCCTCTCACAATCATCAATGAGCTGTTTCCTAATGGTTGGTGGTATGCGGCCGTGGCTTCAATTCAT GTGCTGATGAATATTCTTGTTAGAGGCTTCACTCGCCTATATTGACTCTGTGGATCGGAAACTTAATGAGACACA ATCCAAATTTCAGAAAGAGATAGATGAACTTCAAGAGGAATTGAAAAAGAACCAGGATCCCGAGCGTATGTCCAT GATTCAAGAGATGATTTCCGTAAGTACTGGTCACCGTTCCTTTTAACTTGACATTGTTCTCAACATTTTTAGGAC CTCTTGGGACAAATGTCACGTATTCGTGAAAAAGCTACTGAATCGGAAGCAGTTGTCAGGAATATTACGAAGGAC ATACAGGTCCTGGATCTCGCGAAGAAGAATTTGATCCTTAGTATGACTGTATTAAGACGGTTGCAGATGCTAGGT TCGTTCTCTAAGTTTCTTTCTTTTCCTAATCCACCTCACCTACTTTCCTCCAGTCAATGCAATGACGCAGCTCCA GGATTACATCAAGGAAAAGAGGTACAAAGAAGTATCCGAATCCCTAGCCGTGCGTCATAGGTGTTAATCATCAAC GCCAGTAATATTCAACAGTGCCGTCGATGAATAGACTGTCAAAGAAATATCCGCTACTTTCAAACAATACACATC AGTTCAACGAATCTCAAGAGTCTGGAAACGAATACAAGAATTGCAAGGGGAGTTACGCACTCAGTTAGATGCAGA CTTCGACGCCTTGTATGTCTACCTGTATCCTACCAGTCAATCTCATCTTCTGATTGCATCAAAGCTTTATTCAGG ATTCCACGAAACAAGTCAAACCCGCTTTGATAACTCAAGCTTGTCTCGTCGTCGACGTTCTAGGTTCTGATTTTC GTACCCACCTCATAGACCGTCACGTTGCACTCGAACTCAAAGAATACCGACGTATATTCAAAACCAACGATGAAG CTGGCCAGTTGGATAATATCTCGAGACGTTTCGCTTGGTTCAGGCGGTTGATACAGAACCATGAGCTTGAACAGG GTAGGGTGTTTCCATCGCTTTGGAGAGTTGGGTGGTACTTGTTAGCCAAATTCGCCGAGATCACAAGGTATGTGC GCGTCTGTTTTCTTGTGGCTTTGCGGTCCTGACTTGTCATAACTAAGGGATGATATTTCGGCGTTGCTGTCAAAG GCGGCATCGAATATGACTGTGAAAACGTTACTCGATTATCTACAACAGACCATAGAGTTCGAGCTTTCAATGGCG AAGAAATGGGCAACCCCCGTGAGTTGTGTGCTGCCTTGTATGGCCGACCACATGCTTACTGTTGTTTTACAAGTT TGCGGAAATATTGAAGTTTACCAACGGCTCCAGTCAGCCGCAGAAAGTTATATCCTCATCCTTTGAGCCTCATCT TGGCATATTCGTCGATGCTCAAGACAAGTCAGTTTATCCAGTGTCTTCCGCCTTACGGGTATTGAATAACAGTTC TAGAGTTTTGGCAGAGATGCTGGCACCACATCGAAAGAAGGGAAAACCTCCACCGAGGACGTCCCTTGAAACTAA CCGTGCATCGACTGAAGAAAATGCAGATGACGAACCACCAATAGTTGTCTTACCCTCATCTACAGAATTGTTTTA TCTTTATGGACAGAGTTTGGAGCAATGTGCGAAATTATCGACTGGGAAAACATTGTTTGACCTATGTAATCTGCA CAAGAAGTGGTTGAGAATATATGCAGGTATGTCATGATTGCTTTTTTCTGTTCTTTTTGCTCATATGCGTCTCCT TTTTAGAGGATGTCTTGTCATTGAGCAATCCCAAACAACGGTCAGCATATTTCAAATACTTCCTCGGTGATAGAC TGATGTTTCTTTTTAACTTGAAAGTCCCACGGCGCGTAGATCCATGGAATCGCGATTTGACATAAACGAAATTAA ACGAGCGTGTTTGGTCATCAATACCGCGGACTATTGCCAAACTACAGCGTCCGAGGTAGAGAATTCATCTTTCCG ATGCAAGCGCGCACGAATTCATTTCTTAATTCACTCTAGCTCGAAGAGAAAATCAAGGAAAAGATTAATGAAGAT TTCAAAGAGAAAATCACACTGCAAAAGGAACGTGATCTCTTTGTCAGGTATCCCGTCTCCTGTTCCTTCTTCCAC TTCCGCTCTAATTCCTCTACCTTTATTCTATTTCCTATTCATCCCTGTCGTTAGTGTCATATCATCAGCTATAAC TCTCCTCCTTCGCGAAGTTGAGAACGCCACTGATGTATATTTTACGACAATGATCCGTTCGAATTGGCCAGCTTT GAACCAAGTCAGCGGGCAGTCACATTATGTTGGGGAATTGGTCAAGGCTACGGAACAGGTGGTGGAGACTATCAA ACCGTTGATCGAGCAAAAAAGATATCTGAGGAATTTTCTGGATAAAGTTTGTAGGTGAGTCAGACTTTTTCTCTT GTTTTTTTTTTTCACTTTGCCGCGGGGTTCGCTCTAATAATGACGTTCATCTCTCTGATTTCTAGTGTCGTGTTG GCCAAATTTACAAATGCGCTTGTGAGAAGTAAACCATTAAAAGAGATTGGTGCCGAACAGGTCAGTGGCGTTCTT TCAGCGGTTGTTGTCAAGGTCTATTGATTAACTGTCAAAACCGACTTAGCTCCTACTTGACTTACAAGTTCTCAA AGGGTATCTTACTAAGATGCCGGGAGAGAATTTGTTAACGAATATGTCAGCCTTTACATACCATACTCCTCTTCA CTTCAATCTTTTCTGACGTGAGCCACATGTTCTTCTTCTTCTGTCTGACAGTTACTCGAAGGCGTTAACCAAAAC GGCGACACGACTTGAGACGCTTCTTAAAGTCATCATAACTCCAATGGTACGTCGTTCGGATCTTTTGAATTTTTG TGTGCCTTGGTTTGACATGGGAGAACGGTTACTCTTTTTTATCAAGGACCCAAAAGAGAACTTTATACTCAACTA TACATTCCTCATCGGAGATGCTTCACTAATCAACTTCCAAAAGGTAACCAAAAAAATAAATTCTTGTTGTTTCAG AGGAATAAATTCATTAATTTTATCCTTTTCTAGATTCTTGACCTAAAAGGAGTCCCGCGCACTCTTCATCAAGAT TTCGAAGACACATTCTTGATGATCACGAGTACCAAGACCGATTTAGACAGTACTTCTTTCTTATCTTCGCTCGAT ATGGATCCCCAAGGAGGTGGAGGAGGAGGAAGCAATCTTCTCCTTCTCAGTCCTACAGCTAGTCGAGTTGCATTA CCGCTTGGATCAAATACCATTAGCGATGGTGGTGGTTTATTTCATAGTTTAAGCGGCGGGAGTGGTGGTGCGTCG GTATCCGGTTCGAGTGGACCAGCGACGGGTTCGAGTGTTGGTGGTGGGGTGGGTGGTGTTGGTACTGGCGAAGGC GGTTTTGGTCCTGGAGCGAGAGGAATGTCGGGTGAGACGAATGTCTTTTCTGATTTTAGAAGATTTGTTAGTTTT GGATTGAGGAAAGAGTCGACTCCGCCTTCATAA

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