Fungal Genomics

Agaricus bisporus var bisporus H39 Agaricus bisporus var bisporus H97 Agaricus bisporus var burnettii H119p4 Aspergillus niger CBS 112.32 Aspergillus niger CBS 113.50 Aspergillus niger CBS 115988 Aspergillus niger CBS 115989 Aspergillus niger CBS 118.52 Aspergillus niger CBS 124.48 Aspergillus niger CBS 131.52 Aspergillus niger CBS 133816 Aspergillus niger CBS 147320 Aspergillus niger CBS 147321 Aspergillus niger CBS 147322 Aspergillus niger CBS 147323 Aspergillus niger CBS 147324 Aspergillus niger CBS 147343 Aspergillus niger CBS 147344 Aspergillus niger CBS 147345 Aspergillus niger CBS 147346 Aspergillus niger CBS 147347 Aspergillus niger CBS 147352 Aspergillus niger CBS 147353 Aspergillus niger CBS 147371 Aspergillus niger CBS 147482 Aspergillus niger CBS 630.78 Aspergillus niger CBS 769.97 Niveomyces coronatus CBS 149186 Ophiocordyceps australis 1348a (Ghana) Ophiocordyceps australis map64 (Brazil) Ophiocordyceps camponoti-floridani Ophiocordyceps camponoti-rufipedis Ophiocordyceps kimflemingae Ophiocordyceps subramaniannii Penicillium roqueforti DTO002I6 Penicillium roqueforti DTO003C3 Penicillium roqueforti DTO003H1 (CBS 147308) Penicillium roqueforti DTO006G1 Penicillium roqueforti DTO006G7 Penicillium roqueforti DTO012A1 Penicillium roqueforti DTO012A2 (CBS 147309) Penicillium roqueforti DTO012A6 (CBS 147310) Penicillium roqueforti DTO012A7 Penicillium roqueforti DTO012A8 Penicillium roqueforti DTO012A9 Penicillium roqueforti DTO013E5 Penicillium roqueforti DTO013F2 Penicillium roqueforti DTO013F5 (CBS 147311) Penicillium roqueforti DTO027I6 Penicillium roqueforti DTO032C6 Penicillium roqueforti DTO039G3 Penicillium roqueforti DTO046C5 Penicillium roqueforti DTO070G2 (CBS 147317) Penicillium roqueforti DTO081F9 (CBS 147318) Penicillium roqueforti DTO101D6 (CBS 147325) Penicillium roqueforti DTO102I9 (CBS 147326) Penicillium roqueforti DTO126G2 (CBS 147330) Penicillium roqueforti DTO127F7 (CBS 147331) Penicillium roqueforti DTO127F9 (CBS 147332) Penicillium roqueforti DTO130C1 (CBS 147333) Penicillium roqueforti DTO163C3 (CBS 147337) Penicillium roqueforti DTO163F5 (CBS 147338) Penicillium roqueforti DTO163G4 (CBS 147339) Penicillium roqueforti DTO265D5 (CBS 147372) Penicillium roqueforti DTO369A1 (CBS 147354) Penicillium roqueforti DTO375B1 (CBS 147355) Penicillium roqueforti DTO377G2 (LCP 96.3914a) Penicillium roqueforti LCP96 04111 Rhodonia placenta FPRL280 Torrubiellomyces zombiae CBS 149187

General Properties

Protein ID	AgabiH97|006830
Gene name
Location	scaffold_1:1653763..1656913
Strand	+
Gene length (bp)	3150
Transcript length (bp)	2589
Coding sequence length (bp)	2589
Protein length (aa)	863

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PFAM Domains

PFAM Domain ID	Short name	Long name	E-value	Start	End
PF02278	Lyase_8	Polysaccharide lyase family 8, super-sandwich domain	8.0E-70	486	734
PF08124	Lyase_8_N	Polysaccharide lyase family 8, N terminal alpha-helical domain	3.6E-15	248	404
PF02884	Lyase_8_C	Polysaccharide lyase family 8, C-terminal beta-sandwich domain	4.9E-13	751	821

Swissprot hits

Swissprot ID	Swissprot Description	Start	End	E-value
sp|Q59288|CSLA_PEDHD	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / NCIB 9290) GN=cslA PE=1 SV=1	128	858	3.0E-40
sp|Q9AQS0|XANLY_BACGL	Xanthan lyase OS=Bacillus sp. (strain GL1) GN=xly PE=1 SV=1	185	774	6.0E-11

GO

GO Term	Description	Terminal node
GO:0005576	extracellular region	Yes
GO:0016829	lyase activity	Yes
GO:0005575	cellular_component	No
GO:0003674	molecular_function	No
GO:0003824	catalytic activity	No
GO:0110165	cellular anatomical entity	No

SignalP

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SignalP signal predicted	Location (based on Ymax)	D score (significance: > 0.45)
Yes	1 - 31	0.45

Transmembrane Domains

(None)

Transcription Factor Class

(None)

Expression data

Analysis 1: Developmental stages of Agaricus bisporus (strain A15). Published in Pelkmans et al, Applied Microbiology and Biotechnology, 2016

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Sequences

Type of sequence	Sequence
Locus	Download genbank file of locus The gene with 5 kb flanks (if sufficient flanking sequence is available). For use in cloning design programs. NOTE: features (genes or exons) that are only partially contained within the sequence are completely excluded.
Protein	>AgabiH97|006830 MKPLFNLPRTLFFHVSLFLSLLACPDLVSSASTSTSSTSLSSTHGIVVPSGSSSSRVLSSLTTSHVQSASSSLST RISQTSATAPNSSKMTTIATSTFVNPSLNSSATATSTATVTTPSDPAILEEIEMLRTQVIDIIIGPLGQASAIPA WLTSLGPDGKWPDSEVDYTTGCPARRANWPAQTHWQRILVMAAAWHGGLQGGENFVGDENLRKKTLLAIDYWFGR DLTNLECMINGGTPSCPCDNPDNTLWNTNWFSNVILIPNLVGSSCILLNETLTAAQLDHCTKITNRSYDLFLRPF NEVNFLTGANVLDVAKIGIDGALLNPNITQITDAYRRIHEEVVIQPAIKADGIRPDGSFGQHGGVLYNGNYGKDF TNDIVAFEIEAAETRFAANSESRSALALLFEGDHWMIFRNTVTGVLHWDFSALGRFISFPVIDTQATGSIKLNLT QIDVLGRLWDQPALVQFAESLSEHDMNANAGRLIGNRMFYNNDYMVHRGPNYVTTVKMYSTRTKYGECLNSQNIL GFHLSDGAVYTYLKGNEYEDIAVAWDWNLIPGITTDYEATKLTCSSGVTGIEDFVGGVSNDDTGIAVMRYTNPLT GDLNWQKAWFFLEGDVQHTMISNITSTSDSPVFSVLDQRLHDDAIFMDDGEPFRANDGPTPLSSATLWHGGVGYD LSGLGDDDILHVEVGPKSGNWSAIGTSTEPPSTFDLFAAWIEHGTPASSISYSTFPGTSFTGFMGKQAKLRLQSV VNDGSVGAIYDEEHQTVMVVFWEEDGGSVTIRPPRLAPITISADGNIALIYRFSDAAVYVSDPSQTLTEVQVNLE LGTGWKPPFWGFHRSKDFSIFLPTGGLAGSSVRQPLN*
Coding	>AgabiH97|006830 ATGAAGCCTCTCTTCAATCTCCCTCGTACTCTCTTTTTCCACGTCTCTTTGTTCCTTTCCCTCTTGGCGTGCCCC GATCTGGTTTCAAGCGCGTCCACCTCTACATCATCCACCTCTTTATCCTCAACTCATGGCATTGTTGTGCCATCC GGTTCTTCATCATCTCGGGTGCTTTCCTCGCTGACCACCTCACATGTACAATCTGCATCGTCCTCTTTGAGCACT CGTATATCGCAGACATCTGCTACCGCCCCCAATTCATCCAAGATGACGACTATAGCCACTTCAACCTTTGTCAAT CCTTCTTTGAATTCATCTGCCACAGCAACATCCACTGCTACGGTGACTACGCCTTCCGATCCCGCTATACTGGAA GAAATAGAAATGTTGCGAACCCAAGTAATCGACATTATCATTGGCCCACTTGGTCAAGCGAGTGCAATACCTGCT TGGCTGACATCCCTGGGTCCTGATGGCAAATGGCCTGATAGTGAAGTTGACTATACAACTGGCTGCCCTGCCCGC CGGGCCAACTGGCCAGCCCAGACACATTGGCAGCGTATTCTCGTGATGGCAGCTGCATGGCACGGCGGATTACAA GGAGGGGAAAACTTCGTCGGAGATGAAAATCTCCGTAAGAAAACACTACTGGCTATAGACTATTGGTTCGGGCGT GATCTCACCAATCTTGAATGCATGATCAACGGTGGTACTCCGTCTTGTCCATGTGATAATCCTGATAATACCCTG TGGAACACCAACTGGTTTTCTAATGTTATACTGATTCCGAACCTCGTTGGGTCATCATGCATACTGTTGAACGAG ACTTTGACCGCGGCACAGTTGGATCACTGTACAAAAATAACCAACCGCTCATATGATCTCTTCTTGCGCCCATTT AATGAAGTCAACTTCCTGACAGGTGCAAATGTTTTGGATGTCGCCAAAATTGGAATTGATGGGGCTCTACTCAAC CCCAATATCACACAAATAACCGATGCATATCGTCGCATACATGAAGAGGTTGTAATTCAACCAGCTATCAAAGCC GATGGTATCAGACCTGATGGGTCTTTCGGTCAGCATGGTGGTGTTCTCTACAACGGTAACTACGGCAAGGACTTT ACAAACGACATCGTGGCCTTTGAAATTGAGGCGGCCGAGACACGTTTTGCTGCAAATTCCGAGTCTCGATCCGCT CTTGCTCTTCTCTTCGAAGGTGACCATTGGATGATCTTTCGCAACACCGTCACGGGGGTGCTACACTGGGATTTT TCTGCTCTTGGGCGATTTATCAGCTTTCCTGTCATTGATACTCAGGCCACCGGAAGTATCAAACTAAATCTTACT CAGATTGATGTCCTTGGACGGCTTTGGGATCAACCGGCTCTTGTCCAATTTGCCGAAAGTCTTTCTGAACACGAC ATGAATGCCAATGCAGGTCGGCTCATCGGCAATCGTATGTTCTACAACAACGACTACATGGTTCACCGTGGCCCG AACTACGTTACTACTGTTAAGATGTATTCCACAAGAACAAAGTATGGTGAATGTCTAAACTCTCAGAATATATTG GGTTTCCATCTCTCAGACGGAGCTGTATACACCTATTTGAAGGGCAATGAGTATGAAGATATTGCTGTCGCCTGG GACTGGAACCTTATTCCGGGAATTACTACCGACTATGAAGCGACCAAACTTACTTGTTCCAGCGGCGTCACTGGG ATTGAAGATTTCGTTGGTGGTGTGTCTAATGACGATACTGGTATCGCCGTCATGCGCTACACGAATCCCTTGACT GGCGACTTGAATTGGCAAAAAGCGTGGTTTTTCCTCGAGGGCGACGTCCAACACACCATGATTTCCAATATCACA TCCACATCCGACAGCCCTGTCTTCTCCGTACTTGACCAACGTCTCCATGATGACGCTATTTTCATGGATGACGGT GAACCGTTCAGAGCCAATGATGGACCTACTCCTTTGTCTTCAGCGACGCTTTGGCATGGCGGGGTAGGTTATGAC CTGTCGGGCCTTGGAGATGATGACATCCTCCACGTTGAGGTTGGTCCAAAGTCAGGGAACTGGTCAGCGATCGGT ACTTCAACTGAACCACCATCAACATTTGACCTCTTCGCTGCCTGGATTGAACACGGAACGCCCGCATCATCTATT TCCTATTCCACATTCCCCGGTACCAGTTTCACTGGTTTCATGGGCAAGCAGGCCAAGCTTCGTTTGCAATCTGTT GTGAATGACGGGAGCGTGGGGGCCATATACGACGAGGAACACCAAACAGTAATGGTTGTGTTTTGGGAAGAAGAT GGTGGTAGTGTTACAATCAGACCCCCGAGATTGGCCCCGATCACTATTAGCGCGGACGGCAACATAGCTCTGATT TATCGGTTTTCAGACGCTGCAGTTTATGTGTCAGATCCTTCTCAAACACTTACGGAAGTACAAGTCAATTTGGAA TTGGGTACGGGCTGGAAGCCACCATTCTGGGGTTTCCACAGGTCCAAAGACTTCAGTATATTCCTTCCAACGGGT GGCCTAGCAGGTAGTAGCGTGCGCCAGCCATTGAATTAG
Transcript	>AgabiH97|006830 ATGAAGCCTCTCTTCAATCTCCCTCGTACTCTCTTTTTCCACGTCTCTTTGTTCCTTTCCCTCTTGGCGTGCCCC GATCTGGTTTCAAGCGCGTCCACCTCTACATCATCCACCTCTTTATCCTCAACTCATGGCATTGTTGTGCCATCC GGTTCTTCATCATCTCGGGTGCTTTCCTCGCTGACCACCTCACATGTACAATCTGCATCGTCCTCTTTGAGCACT CGTATATCGCAGACATCTGCTACCGCCCCCAATTCATCCAAGATGACGACTATAGCCACTTCAACCTTTGTCAAT CCTTCTTTGAATTCATCTGCCACAGCAACATCCACTGCTACGGTGACTACGCCTTCCGATCCCGCTATACTGGAA GAAATAGAAATGTTGCGAACCCAAGTAATCGACATTATCATTGGCCCACTTGGTCAAGCGAGTGCAATACCTGCT TGGCTGACATCCCTGGGTCCTGATGGCAAATGGCCTGATAGTGAAGTTGACTATACAACTGGCTGCCCTGCCCGC CGGGCCAACTGGCCAGCCCAGACACATTGGCAGCGTATTCTCGTGATGGCAGCTGCATGGCACGGCGGATTACAA GGAGGGGAAAACTTCGTCGGAGATGAAAATCTCCGTAAGAAAACACTACTGGCTATAGACTATTGGTTCGGGCGT GATCTCACCAATCTTGAATGCATGATCAACGGTGGTACTCCGTCTTGTCCATGTGATAATCCTGATAATACCCTG TGGAACACCAACTGGTTTTCTAATGTTATACTGATTCCGAACCTCGTTGGGTCATCATGCATACTGTTGAACGAG ACTTTGACCGCGGCACAGTTGGATCACTGTACAAAAATAACCAACCGCTCATATGATCTCTTCTTGCGCCCATTT AATGAAGTCAACTTCCTGACAGGTGCAAATGTTTTGGATGTCGCCAAAATTGGAATTGATGGGGCTCTACTCAAC CCCAATATCACACAAATAACCGATGCATATCGTCGCATACATGAAGAGGTTGTAATTCAACCAGCTATCAAAGCC GATGGTATCAGACCTGATGGGTCTTTCGGTCAGCATGGTGGTGTTCTCTACAACGGTAACTACGGCAAGGACTTT ACAAACGACATCGTGGCCTTTGAAATTGAGGCGGCCGAGACACGTTTTGCTGCAAATTCCGAGTCTCGATCCGCT CTTGCTCTTCTCTTCGAAGGTGACCATTGGATGATCTTTCGCAACACCGTCACGGGGGTGCTACACTGGGATTTT TCTGCTCTTGGGCGATTTATCAGCTTTCCTGTCATTGATACTCAGGCCACCGGAAGTATCAAACTAAATCTTACT CAGATTGATGTCCTTGGACGGCTTTGGGATCAACCGGCTCTTGTCCAATTTGCCGAAAGTCTTTCTGAACACGAC ATGAATGCCAATGCAGGTCGGCTCATCGGCAATCGTATGTTCTACAACAACGACTACATGGTTCACCGTGGCCCG AACTACGTTACTACTGTTAAGATGTATTCCACAAGAACAAAGTATGGTGAATGTCTAAACTCTCAGAATATATTG GGTTTCCATCTCTCAGACGGAGCTGTATACACCTATTTGAAGGGCAATGAGTATGAAGATATTGCTGTCGCCTGG GACTGGAACCTTATTCCGGGAATTACTACCGACTATGAAGCGACCAAACTTACTTGTTCCAGCGGCGTCACTGGG ATTGAAGATTTCGTTGGTGGTGTGTCTAATGACGATACTGGTATCGCCGTCATGCGCTACACGAATCCCTTGACT GGCGACTTGAATTGGCAAAAAGCGTGGTTTTTCCTCGAGGGCGACGTCCAACACACCATGATTTCCAATATCACA TCCACATCCGACAGCCCTGTCTTCTCCGTACTTGACCAACGTCTCCATGATGACGCTATTTTCATGGATGACGGT GAACCGTTCAGAGCCAATGATGGACCTACTCCTTTGTCTTCAGCGACGCTTTGGCATGGCGGGGTAGGTTATGAC CTGTCGGGCCTTGGAGATGATGACATCCTCCACGTTGAGGTTGGTCCAAAGTCAGGGAACTGGTCAGCGATCGGT ACTTCAACTGAACCACCATCAACATTTGACCTCTTCGCTGCCTGGATTGAACACGGAACGCCCGCATCATCTATT TCCTATTCCACATTCCCCGGTACCAGTTTCACTGGTTTCATGGGCAAGCAGGCCAAGCTTCGTTTGCAATCTGTT GTGAATGACGGGAGCGTGGGGGCCATATACGACGAGGAACACCAAACAGTAATGGTTGTGTTTTGGGAAGAAGAT GGTGGTAGTGTTACAATCAGACCCCCGAGATTGGCCCCGATCACTATTAGCGCGGACGGCAACATAGCTCTGATT TATCGGTTTTCAGACGCTGCAGTTTATGTGTCAGATCCTTCTCAAACACTTACGGAAGTACAAGTCAATTTGGAA TTGGGTACGGGCTGGAAGCCACCATTCTGGGGTTTCCACAGGTCCAAAGACTTCAGTATATTCCTTCCAACGGGT GGCCTAGCAGGTAGTAGCGTGCGCCAGCCATTGAATTAG
Gene	>AgabiH97|006830 ATGAAGCCTCTCTTCAATCTCCCTCGTACTCTCTTTTTCCACGTCTCTTTGTTCCTTTCCCTCTTGGCGTGCCCC GATCTGGTTTCAAGCGCGTCCACCTCTACATCATCCACCTCTTTATCCTCAACTCATGGCATTGTTGTGCCATCC GGTTCTTCATCATCTCGGGTGCTTTCCTCGCTGACCACCTCACATGTACAATCTGCATCGTCCTCTTTGAGCACT CGTATATCGCAGACATCTGCTACCGCCCCCAATTCATCCAAGATGACGACTATAGCCACTTCAACCTTTGTCAAT CCTTCTTTGAATTCATCTGCCACAGCAACATCCACTGCTACGGTGACTACGCCTTCCGATCCCGCTATACTGGAA GAAATAGAAATGTTGCGAACCCAAGTAATCGACATTATCATTGGCCCACTTGGTCAAGCGAGTGCAATACCTGCT TGGTTTGTGTTGAACATTCTAACCAGGTCTCGAACAGCAATAGCTAATCCACGCTAGGCTGACATCCCTGGGTCC TGATGGCAAATGGCCTGATAGTGAAGTTGACTATACAACTGGCTGCCCTGCCCGCCGGGCCAACTGGCCAGCCCA GACACATTGGCAGCGTATTCGTGAGTTCTTGCTTGGGGCGATCGTGGAAAAGCCTTCTCATGAATTTTAGTCGTG ATGGCAGCTGCATGGCACGGCGGATTACAAGGAGGGGAAAACTTCGTCGGAGATGAAAATCTCCGTAAGAAAACA CTACTGGCTATAGACTATTGGTTCGGGCGTGATCTCACCAATCTTGAATGCATGATCAACGGTGGTACTCCGTCT TGTCCATGTGATAATCCTGATAATACCCTGTGGTATGAAGATGCTGCATTGAAATAAGGGCCACTACTGAAATAT TTTTTCAAGGAACACCAACTGGTTTTCTAATGTTCGTCGCTGTCTTCTGTTTAGCACCCATCACCAATTGATTGT ACTAGGTTATACTGATTCCGAACCTCGTTGGGTCATCATGCATACTGTTGAACGAGACTTTGACCGCGGCACAGT TGGATCACTGTACAAAAATAACCAACCGCTCATATGATCTCTTCTTGCGCCCATTTAATGAAGTCAACTTCCTGA CAGGTGCAAATGTTTTGGATGTCGCCAAAATTGGAATTGATGGGGCTCTACTCAACCCCAATATCACACAAATAA CCGATGCATATCGTCGCATACATGAAGAGGTTGTAATTCAACCAGCTATCAAAGCCGATGGTATCAGACCTGATG GGTCTTTCGGTGCGATATCATCTGGATTCTGATTCCTTGTCTCATTTCTGTATTTCAGGTCAGCATGGTGGTGTT CTCTACAACGGTAACTACGGTAAGTGGGGTTGATTGAGTTCATAATATGTTTCGTGTGCTCAGGTTTTTTGCAGG CAAGGACTTGTGAGTGGAGAATTGAAATTGAGGAACTCTCATATTGATTCTTCGAAGTACAAACGACATCGTGGC CTTTGAAATTGAGGCGGCCGAGACACGTTTTGCTGCAAATTCCGAGTCTCGATCCGCTCTTGCTCTTCTCTTCGA AGGTGACCATTGGATGATCTTTCGCAACACCGTCACGGGGGTGCTACACTGGGATTTTGTGAGTACCTCACTAAT CTCATTTGACTCTTACTCAGTCAGGTCTAGTCTGCTCTTGGGCGATTTATCAGCTTTCCTGTCATTGATACTCAG TACGTTCTACTCTACTTTTTCCTATCAGATAAATGTAAAACCATCTCTTTAGGGCCACCGGAAGTATCAAACTAA ATCTTACTCAGATTGATGTCCTTGGACGGCTTTGGGATCAACCGGCTCTTGTCCAATTTGCCGAAAGTCTTTCTG AACACGACATGAATGCCAATGCAGGTCGGCTCATCGGCAATCGTATGTTCTACAACAACGACTACATGGTTAGCT TCCTCGCCTCCAAGCCTAGATTCTGATTTATAGTTCGTCAGGTTCACCGTGGCCCGAACTACGTTACTACTGTTA AGATGTATTCCACAAGAACAAAGTATGGTGAATGTCTAAACTCTCAGAATGTATGTATTGTTGGACTTCCATTGT TCGGGCTCTCCTTCATCGATACTCCTTTAGATATTGGGTTTCCATCTCTCAGACGGAGCTGTATACACCTATTTG AAGGGCAATGAGTATGAAGATATTGCTGTCGCCTGGGACTGGAACCTTATTCCGGGAATTACTACCGACTATGAA GCGACCAAACTTACTTGTTCCAGCGGCGTCACTGGGATTGAAGATTTCGTTGGTGGTGTGTCTAATGACGATACT GGTATCGCCGTCATGCGCTACACGAATCCCTTGACTGGCGACTTGAATTGGCAAAAAGCGTGGTTTTTCCTCGAG GGCGACGTCCAACACACCATGATTTCCAATATCACATCCACATCCGACAGCCCTGTCTTCTCCGTACTTGACCAA CGTCTCCATGATGACGCTATTTTCATGGATGACGGTGAACCGTTCAGAGCCAATGATGGACCTACTCCTTTGTCT TCAGCGACGCTTTGGCATGGCGGGGTAGGTTATGACCTGTCGGGCCTTGGAGATGATGACATCCTCCACGTTGAG GTTGGTCCAAAGTCAGGGAACTGGTCAGCGATCGGTACTTCAACTGAACCACCATCAACATTTGACCTCTTCGCT GCCTGGATTGAACACGGAACGCCCGCATCATCTATTTCCTATTCCACATTCCCCGGTACCAGTTTCACTGGTTTC ATGGGCAAGCAGGCCAAGCTTCGTTTGCAATCTGTTGTGAATGACGGGAGCGTGGGGGCCATATACGACGAGGAA CACCAAACAGTAATGGTTGTGTTTTGGGAAGAAGATGGTGGTAGTGTTACAATCAGACCCCCGAGATTGGCCCCG ATCACTATTAGCGCGGACGGCAACATAGCTCTGATTTATCGGTTTTCAGACGCTGCAGTTTATGTGTCAGATCCT TCTCAAACACTTACGGAAGTACAAGTCAATTTGGAATTGGGTACGGGCTGGAAGCCACCATTCTGGGGTTTCCAC AGGTCCAAAGACTTCAGTATATTCCTTCCAACGGGTGGCCTAGCAGGTAGTAGCGTGCGCCAGCCATTGAATTAG